Abstract:
:The validity and feasibility of using DNA collection cards in the field for preservation and analysis of Cannabis sativa genotypes were investigated using a highly specific hexanucleotide marker. Collection cards were submitted to the National Marijuana Initiative, which selectively trained and managed the collection of specific types of samples from a variety of participating agencies. Samples collected at seizure sites included fresh marijuana leaf samples, dried "dispensary" samples, U.S. border seizures, and hashish. Using a standardized PCR kit with custom-labeled oligonucleotide primers specific to marijuana, collection cards produced eight genotypes and 13 different alleles, extremely low baselines, and no cross-reactivity with control plant species. Results were produced from all sample types with the exception of hashish. Plant DNA collection cards represent an easily implementable method for the genetic identification and relatedness of C. sativa street and grow site-seized samples with applications for databasing and market disruption.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Allgeier L,Hemenway J,Shirley N,LaNier T,Coyle HMdoi
10.1111/j.1556-4029.2011.01818.xsubject
Has Abstractpub_date
2011-09-01 00:00:00pages
1245-9issue
5eissn
0022-1198issn
1556-4029journal_volume
56pub_type
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