Abstract：
:Slimming cactus (Hoodia), found only in southwestern Africa, is a well-known herbal product for losing weight. Consequently, Hoodia extracts are sought-after worldwide despite a CITES Appendix II status. The failure to eradicate illegal trade is due to problems with detecting and identifying Hoodia using morphological and chemical characters. Our aim was to evaluate the potential of molecular identification of Hoodia based on DNA barcoding. Screening of nrITS1 and psbA-trnH DNA sequences from 26 accessions of Ceropegieae resulted in successful identification, while conventional chemical profiling using DLI-MS led to inaccurate detection and identification of Hoodia. The presence of Hoodia in herbal products was also successfully established using DNA sequences. A validation procedure of our DNA barcoding protocol demonstrated its robustness to changes in PCR conditions. We conclude that DNA barcoding is an effective tool for Hoodia detection and identification which can contribute to preventing illegal trade.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Gathier G,van der Niet T,Peelen T,van Vugt RR,Eurlings MC,Gravendeel Bdoi
10.1111/1556-4029.12184subject
Has Abstractpub_date
2013-11-01 00:00:00pages
1467-71issue
6eissn
0022-1198issn
1556-4029journal_volume
58pub_type
杂志文章abstract：:It is thought that, in a consignment of discrete units, a certain proportion of the units contain illegal material. A sample of the consignment is to be inspected. Various methods for the determination of the sample size are compared. The consignment will be considered as a random sample from some super-population of ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1999-07-01 00:00:00
abstract：:Phencyclidine (PCP) can be detected in human hair wih commercially available radioimmunoassay regents. hair samples of all subjects admitting PCP use were positive, while thin-layer chromatographic urine analyses were positive in only one of seven cases. Presumably the drug is incorporated into the hair during period...
journal_title：Journal of forensic sciences
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doi：
更新日期：1981-07-01 00:00:00
abstract：:An unusual fatality involving a 26-year-old male who intentionally ingested approximately 10 g of metoprolol is presented. Autopsy findings revealed foreign material in the gastric content with an acute thrombosis of the left anterior descending coronary artery. Microscopic studies showed evidence of an extremely earl...
journal_title：Journal of forensic sciences
pub_type： 杂志文章,评审
doi：
更新日期：1987-09-01 00:00:00
abstract：:The United States Supreme Court, in the recent case of Riggins v. Nevada, extended its examination of the issue of involuntary treatment with anti-psychotic medication to the mentally disabled facing criminal trial. Although this was an extreme case where the defendant faced a possible death sentence, the involuntary ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
abstract：:A forensic procedure for the quantitative confirmation of lysergic acid diethylamide (LSD) and the qualitative confirmation of its metabolite, N-demethyl-LSD, in blood, serum, plasma, and urine samples is presented. The Zymark RapidTrace was used to perform fully automated solid-phase extractions of all specimen types...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-05-01 00:00:00
abstract：:The concentrations of antimony, copper, tin, arsenic, silver, bismuth, and cadmium in lead alloys produced by two smelters and one ammunition manufacturer were determined using inductively coupled plasma-atomic emission spectrometry. These element concentrations were used to measure the variations in composition of le...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2002-09-01 00:00:00
abstract：:Sex determination of the human skeleton is best assessed from the os coxa. The present study explored the possibility of using three-dimensional landmark coordinate data collected from various landmarks located over the entire bone to determine whether there were significant sex differences local to the landmarks. Thi...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01374.x
更新日期：2010-07-01 00:00:00
abstract：:A sample of 31 criminal defendants who were charged with murder but without major mental disorder was studied. The sample was derived from a large urban multi-ethnic, multi-cultural community pool. Subject characteristics and information about the homicide and decedents are described. Suggestions for further study are...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-09-01 00:00:00
abstract：:Many studies have been published describing numerous techniques to determine the aging of individuals from dental tissues. However, few case reports demonstrate how such techniques can be employed by the forensic odontologist undertaking casework. Indeed, many of the techniques are highly complex and utilize equipment...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：:A closed case file review of a nonrandom national sample of 975 homicides disclosed 11 cases (1.13%) of undoing, wherein offenders engaged in crime scene behavior that has been considered an attempt to symbolically reverse the murder. The frequency of the various methods of undoing involved the use of blankets to cove...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13556
更新日期：2018-03-01 00:00:00
abstract：:Respiratory pathogens have been detected in forensic investigations using multiple techniques; however, no study has examined the use of automated, nested, multiplex polymerase chain reaction (ANM-PCR), commonly used in living patients, in the forensic setting. This retrospective study assessed the utility of ANM-PCR ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13415
更新日期：2017-09-01 00:00:00
abstract：:Pharmacokinetic studies of psilocybin in humans have shown the rapid dephosphorylation of psilocybin to psilocin with further conversion to 4-hydroxy-tryptophole (4HT) and 4-hydroxyindole-3-acetic acid (4HIAA) in plasma. Our study shows that psilocin also undergoes conjugation and can be found in the urine as the psil...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2001-05-01 00:00:00
abstract：:The near inevitable transfer of explosive particulate matter through fingerprints makes it possible to detect concealed explosives through surface sampling. Repeatable and well-characterized fingerprint simulation facilitates quantitative comparison between particulate sampling methods for subsequent detection of trac...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2000-07-01 00:00:00
abstract：:Field studies in forensic entomology seem to assume that a cadaver is a resource equally accessible to flies, irrespective of the species' size, behavior, and sensorial capacity. We aimed to challenge this assumption by experimentally manipulating baited traps in order to test whether the accessibility to decomposing ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14328
更新日期：2020-09-01 00:00:00
abstract：:Internal standards are commonly used for the quantitative determination of drugs of abuse and their metabolites (drug/metabolite) in biological fluids and tissues by the selective ion monitoring (SIM) gas chromatography/mass spectrometry (GC/MS) procedure. Analogs of drugs/metabolites that are labeled with three or mo...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-11-01 00:00:00
abstract：:Deoxyribonucleic acid (DNA) typing of ribosomal ribonucleic acid (rRNA) genes was performed with a polymerase chain reaction (PCR) assay for species identification. A variable region of the 28S ribosomal RNA gene was amplified with primers complementary to flanking sequences phylogenetically well conserved. The produc...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1992-03-01 00:00:00
abstract：:The purpose of this study was to investigate the retention of blood stains on twelve different types of fabrics after washing at various drying times. The findings of this study, supported by chi-square analysis, indicate that the retention of bloodstains on washed fabrics depends upon the particular fiber composition...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1990-11-01 00:00:00
abstract：:The sale of fraudulent oriental ceramics constitutes a large proportion of the illegal artifact and antique trade and threatens to undermine the legitimate international market. The sophistication and skill of forgers has reached a level where, using traditional appraisal by eye and hand, even the most experienced spe...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2006.00364.x
更新日期：2007-03-01 00:00:00
abstract：:Most false aneurysms of the heart represent contained ventricular free wall ruptures after myocardial infarction. Post-traumatic aneurysms also may follow penetrating or non-penetrating trauma to the chest. Regardless of the origin of the false aneurysm there is a propensity for aneurysm rupture. We report a patient w...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2001-03-01 00:00:00
abstract：:The current clandestine methodology for the manufacture of illicit cocaine hydrochloride utilizes microwave heating in order to dry the finished product. This study addresses the effects this step has on the occluded solvents present in newly prepared cocaine hydrochloride. Nine 1-kilogram-sized batches of cocaine hyd...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2000-09-01 00:00:00
abstract：:Over the past decade, there has been a growing openness about the importance of human factors in forensic work. However, most of it focused on cognitive bias, and neglected issues of workplace wellness and stress. Forensic scientists work in a dynamic environment that includes common workplace pressures such as worklo...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13533
更新日期：2018-01-01 00:00:00
abstract：:Attenuated total reflection (ATR) is a convenient mode for single fiber analysis by infrared microspectroscopy, particularly when transmission spectra are difficult to obtain or when surface preferenced sampling is desirable. Textile finishes such as spin finishes, anti-static finishes, and permanent press finishes ar...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:The purpose of the present investigation was to test the accuracy of a new scoring system in recording tooth wear for age estimation. The material consisted of dental stone casts of 383 volunteers who had sound premolars and molars with normal occlusion. The degree of occlusal wear for all premolar and molar teeth was...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:Little is known about the racial patterns of crimes committed by sexual homicide offenders (SHOs). This study examined race and age influences on victim-offender relationship for juvenile and adult SHOs. A large sample (N = 3868) from the Supplemental Homicide Reports (1976-2005) was used. Analyses of victim-offender ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01448.x
更新日期：2010-09-01 00:00:00
abstract：:The ability to correctly estimate the sex of skeletal remains is vital in forensic sciences. This article investigates the sexual dimorphism of the human sacro-iliac joint, using geometric morphometric techniques that assess morphological characters better than the traditional approaches for recording outline shapes, ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2012.02282.x
更新日期：2013-01-01 00:00:00
abstract：:Although the change in adenosine phosphate levels in muscles may contribute to the development of rigor mortis, the relationship between their levels and the onset and development of rigor mortis has not been well elucidated. In the current study, levels of the adenosine phosphates including adenosine triphosphate (AT...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01450.x
更新日期：2010-09-01 00:00:00
abstract：:Physical matching methods are a family of well-known methods that are utilized in order to determine whether two pieces of a tearable/breakable material used to be one object. When performing physical match of polymers, it is sometimes difficult to reach an unambiguous conclusion. This is due to various reasons. One i...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14561
更新日期：2021-01-01 00:00:00
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journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:A procedure for the multisystem analysis of bloodstains using the simultaneous separation of the enzymes glyoxalase I, esterase D, and phosphoglucomutase has been developed. The amount of bloodstain required has therefore been reduced threefold without any loss in resolution and sensitivity. Bloodstains at least seven...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1986-10-01 00:00:00
abstract：:The contribution of a physical anthropologist to a forensic investigation is generally associated with analysis of skeletal remains in a laboratory. This case, which deals with the identification of two U.S. journalists who disappeared in Guatemala in March 1985, shows that the observations of a physical anthropologis...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1993-11-01 00:00:00
