Abstract：
:DNA is often difficult to extract from old bones and teeth due to low levels of DNA and high levels of degradation. This study established a simple yet efficient method for extracting DNA from 20 aged bones and teeth (approximately 60 years old). Based on the concentration and STR typing results, the new method of DNA extraction (OM) developed in this study was compared with the PrepFiler™ BTA Forensic DNA Extraction Kit (BM). The total amount of DNA extracted using the OM method was not significantly different from that extracted using the commercial kit (p > 0.05). However, the number of STR loci detected was significantly higher in the samples processed using the OM method than using the BM method (p < 0.05). This study aimed to establish a DNA extraction method for aged bones and teeth to improve the detection rate of STR typing and reduce costs compared to the BM technique.
journal_name
J Forensic Scijournal_title
Journal of forensic sciencesauthors
Liu Q,Liu L,Zhang M,Zhang Q,Wang Q,Ding X,Shao L,Zhou Z,Wang Sdoi
10.1111/1556-4029.13603subject
Has Abstractpub_date
2018-05-01 00:00:00pages
824-828issue
3eissn
0022-1198issn
1556-4029journal_volume
63pub_type
杂志文章abstract：:In late June 1990, the Mono County Sheriffs Department in Bridgeport, CA contacted the Physical Anthropology Human Identification Laboratory (PAHIL) at California State University, Chico to seek assistance in the identification of a recently discovered skull. To assist with possible identification, the cranium receive...
journal_title：Journal of forensic sciences
pub_type： 历史文章,杂志文章
doi：
更新日期：2000-03-01 00:00:00
abstract：:A sensitive analysis of sulfide in blood was established, using an extractive alkylation technique. Pentafluorobenzyl bromide was used as the alkylating agent, tetradecyldimethylbenzylammonium chloride as the phase-transfer catalyst, and potassium dihydrogenphosphate as the buffer to suppress the formation of sulfide....
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1988-01-01 00:00:00
abstract：:The acetabulum/pubis index was tested on American Eskimo and Indian samples of innominate bones to which sex had been assigned by visual inspection. The index alone sexed 91 and 89% of the Eskimo and Indian samples, respectively. Subsequent sorting of misclassified specimens with femur head diameter resulted in 96 and...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1988-05-01 00:00:00
abstract：:Separation of male and female soft tissue depths into discrete groups for craniofacial identification implies that males and females differ enough from each other, with respect to this application, for this distinction to be useful. In this study, previously published soft tissue depth data were analyzed for sex separ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2005-05-01 00:00:00
abstract：:We present in this work a study about biochemical changes of the synovial liquid in 100 corpses with regard to the cause of death. The results obtained in the different groups of causes of death show that the biochemical parameters were modified postmortem although we think that this modification is related more direc...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1985-04-01 00:00:00
abstract：:Periventricular leukomalacia (PVL) is a form of cerebral infarction occurring in neonates, particularly in low-weight and premature infants. PVL is well-known to neonatologists, but generally considered nonfatal. Many infants with PVL die in the hospital with multiple medical problems. Those infants with PVL who survi...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1985-10-01 00:00:00
abstract：:A state law has placed an obligation on the criminal investigation laboratories to screen and quantitate all preparations, both pharmaceutical and illicit, that fall under the provisions of "Excepted Substances." Without complete screens on these preparations, there is room for erroneous indictments as well as possibl...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1976-04-01 00:00:00
abstract：:We explored the quality distribution of ninhydrin-developed prints on A4 bond paper in two groups of individuals, in Israel and in India. While the quality distributions of the developed marks in both countries had some dissimilarities, both groups showed the expected bell-shape distribution, with the majority of the ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2010.01587.x
更新日期：2011-01-01 00:00:00
abstract：:Reducing amplicon sizes has become a major strategy for analyzing degraded DNA typical of forensic samples. However, amplicon sizes in current mini-short tandem repeat-polymerase chain reaction (PCR) and mini-sequencing assays are still not suitable for analysis of severely degraded DNA. In this study, we present a mu...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2009.01166.x
更新日期：2009-11-01 00:00:00
abstract：:The smokeless powders in 22 kinds of ammunitions seized from one of the Japanese gang groups were analyzed by scanning electron microscopy/energy dispersive X-ray microanalysis (SEM/EDX). Copper(Cu), sulfur(S), potassium(K), silicon(Si), aluminum(Al), calcium(Ca), iron(Fe), chlorine(Cl), and barium(Ba) were detected. ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-01-01 00:00:00
abstract：:Domestic homicide is the most extreme form of domestic violence and one of the most common types of homicide. The objective was to examine differences between spontaneous domestic homicide and nondomestic homicide offenders regarding demographics, psychiatric history, crime characteristics, and neuropsychological stat...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12908
更新日期：2016-01-01 00:00:00
abstract：:This study analyses depressed fractures (by blunt force trauma) and circular full-thickness injuries (drill injuries and gunshot wounds) in charred bones. Fifty bovine ribs (total 104 lesions) were divided into three groups. The first group consisted in 20 depressed hammer-produced fractures; in the second one, 60 rou...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12794
更新日期：2015-09-01 00:00:00
abstract：:Siding and sequencing (that is, putting in anatomical order) human ribs are essential to the proper examination and documentation of injuries to the chest. The paucity of information regarding sequencing nonfleshed human ribs makes it particularly difficult for physical anthropologists and medicolegal authorities to d...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1993-01-01 00:00:00
abstract：:ABO types obtained from evidentiary samples have been used effectively to obtain the initial information leading to the apprehension of culprits in Japanese criminal investigations. A simple ABO genotyping method using multiplex sequence-specific PCR and capillary electrophoresis was developed as a supplement to serol...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:Mitragyna speciosa (MS), a plant commonly known as kratom, is a widely used "legal high" opiate alternative for pain relief. DNA extracted from MS and 26 additional plant species was amplified by PCR using primers targeting the strictosidine beta-D-glucosidase (SGD) and secologanin synthase 2 (SLS2) genes and detected...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.14167
更新日期：2020-01-01 00:00:00
abstract：:An improved gas chromatographic/mass spectrometric (GC/MS) assay is described for the quantitation of codeine and morphine as trimethylsyl (TMS) derivatives. The TMS derivatization of ketone-containing opiates results in the formation of multiple derivatives. Some of these products have retention times close to those ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1998-11-01 00:00:00
abstract：:A series of cases is reported in which pink teeth were observed during the postmortem period. Most cases were associated with decomposition in a moist environment. Experimental procedures led to the extraction of pink material from dentin and demonstration that hemoglobin and serum proteins were present. The pink-toot...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1977-01-01 00:00:00
abstract：:This paper constitutes a feasibility report on the use of the human leukocyte antigen (HLA) system for the typing of dried bloodstains. Antigens tested include the HLA-A2, A3, A10, B7, B8, and B14 alleles. An aging study conducted on A3 positive bloodstains showed that HLA-A3 could be reliably detected on bloodstains ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:Many current methods of age determination available to forensic anthropologists are limiting in that the age ranges provided are often broad, particularly for individuals in their late teens to early 20's. This study introduces an improved method for aging teenagers and young adults. The pattern and stages of union of...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:The case of a 48-year-old woman accused of killing at least 12 elderly women and attempting to kill another one during the last 3 years is presented. Extensive neuropsychological, electrophysiological, and neuropsychiatric testing showed no evidence of a DSM-IV-TR Axis I diagnosis, but a decrease in executive function...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2008.00803.x
更新日期：2008-09-01 00:00:00
abstract：:To obtain a reliable estimation on the yield of illicit indoor cannabis cultivation in The Netherlands, cannabis plants confiscated by the police were used to determine the yield of dried female flower buds. The developmental stage of flower buds of the seized plants was described on a scale from 1 to 10 where the val...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2006.00228.x
更新日期：2006-09-01 00:00:00
abstract：:Recent publications from the National Academy of Sciences have called for additional foundational research in the field of firearm and toolmark analysis. We examined test fires from 10 pistol slides with consecutively manufactured breech faces. A total of nine test fires from each pistol slide, for a total of 90 test ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2012.02072.x
更新日期：2012-07-01 00:00:00
abstract：:We report the identification of acetone (0.45 mg/mL) and isopropanol (0.17 mg/mL) but without the presence of ethanol in a blood sample from a man suspected of driving under the influence of alcohol. A preliminary breath screening test with an electrochemical instrument (Alcolmeter S-L2) was positive and an evidential...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:A few sexual fatalities show ambiguous or conflicting evidence of manner of death or, in cases involving partners, of the partner's intent. In such equivocal cases, postmortem behavioral analysis and reconstruction aid in understanding what happened and provide an explainable basis for expert judgment and opinion, eve...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1982-10-01 00:00:00
abstract：:The estimation of postmortem interval (PMI) based on successional patterns of adult insects is largely limited, due to the lack of potential PMI markers. Sex and size of adult insects could be easily used for such estimation. In this study, sex- and size-related patterns of carrion attendance by adult insects were ana...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13376
更新日期：2017-09-01 00:00:00
abstract：:Weight is often one of the few recoverable data when analyzing human cremains but references are still rare, especially for European populations. Mean weights for skeletal remains were thus documented for Portuguese modern cremations of both recently deceased individuals and dry skeletons, and the effect of age, sex, ...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12167
更新日期：2013-09-01 00:00:00
abstract：:Bismuth oxychloride (BiOCl) was the first viable synthetic pearl pigment developed 50 years ago. It was only used for a limited time period in automotive paint (model years 1998-2000), serving to produce luster for a single Chrysler black metallic color. Identification of this pigment in an unknown automotive paint ca...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.12414
更新日期：2014-09-01 00:00:00
abstract：:The detection of DNA is inhibited in cases of sexual assault involving condom use. Trace evidence, including condom lubricant residues, provides crucial associative evidence in such cases. The existing Fourier transform infrared spectroscopy (FTIR) methods for lubricant analysis and detection are limited with regard t...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/j.1556-4029.2007.00411.x
更新日期：2007-05-01 00:00:00
abstract：:The reliability of a D1S80 typing procedure has been evaluated using simulated forensic specimens. D1S80 alleles were detectable in DNA recovered from bloodstains exposed to sunlight for up to 20 weeks. However, D1S80 alleles were undetectable in semen stains after six weeks sunlight exposure. Analysis of blood and se...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：
更新日期：1995-07-01 00:00:00
abstract：:The pubic symphysis is among the most commonly used bilateral age indicators. Because of potential differences between right and left sides, it is necessary to investigate within-individual asymmetry, which can inflate age estimation error. This study uses 3D laser scans of paired pubic symphyses for 88 documented Whi...
journal_title：Journal of forensic sciences
pub_type： 杂志文章
doi：10.1111/1556-4029.13871
更新日期：2019-03-01 00:00:00
