Evolution of protamine P1 genes in mammals.

Abstract:

:Protamine P1 genes have been sequenced following PCR amplification from 11 mammals representing five major mammalian orders: Rodentia (rat and guinea pig), Carnivora (cat and bear), Proboscidea (elephant), Perissodactyla (horse), and Artiodactyla (camel, deer, elk, moose, and gazelle). The predicted amino acid sequence for these genes together with previously reported sequences results in a data set of 25 different P1 genes and 30 different P1 amino acid sequences. The alignment of all these sequences reveals that protamines are amongst the most rapidly diverging proteins studied. In spite of the large number of differences there are conserved motifs that are also common to birds such as the N-terminal ARYR followed by the triple alternating SRSRSR phosphorylation site. The central region contains 3 arginine clusters consisting of 5-6 arginines each. The C-terminus appears to be the most variable region of the protamines. Overall the molecular evolution of P1 genes is in agreement with the expected species evolution supporting that these genes have evolved vertically.

journal_name

J Mol Evol

authors

Queralt R,Adroer R,Oliva R,Winkfein RJ,Retief JD,Dixon GH

doi

10.1007/BF00160507

subject

Has Abstract

pub_date

1995-06-01 00:00:00

pages

601-7

issue

6

eissn

0022-2844

issn

1432-1432

journal_volume

40

pub_type

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