RU486 inhibits ovulation, fertilization and early embryonic development in rabbits: in vivo and in vitro studies.


OBJECTIVE:To clarify the role of P in ovulation, fertilization, and early embryonic development using RU486, a potent P receptor blocker. DESIGN:Ovulatory efficiency, IVF, and early embryonic development were evaluated after RU486 administration in vivo and in vitro. SETTING:Research laboratory of a university hospital. PARTICIPANTS:Mature male and female New Zealand white rabbits. INTERVENTIONS:Animals were treated with RU486 or vehicle for 3 days before hCG-induced ovulation. Ovaries treated with hCG to induce ovulation were perfused for 6 hours in vitro with RU486 or vehicle. In vitro fertilization was performed in the presence or absence of RU486. MAIN OUTCOME MEASURES:The percentage of mature follicles ovulating (ovulatory efficiency) was determined after in vivo and in vitro treatment. Fertilization, morula, and blastocyst development were evaluated every 24 hours for 120 hours. RESULTS:RU486 significantly inhibited ovulation, fertilization, and early embryonic development. CONCLUSIONS:Progesterone plays a significant role in ovulation, fertilization, and preimplantation embryonic development.


Fertil Steril


Fertility and sterility


Chen SH,Dharmarajan AM,Wallach EE,Mastroyannis C




Has Abstract


1995-09-01 00:00:00














  • Assessing the true incidence of mosaicism in preimplantation embryos.

    abstract::Modern technologies applied to the field of preimplantation genetic diagnosis for aneuploidy screening (PGD-A) have improved the ability to identify the presence of mosaicism. Consequently, new questions can now be addressed regarding the potential impact of embryo mosaicism on diagnosis accuracy and the feasibility o...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Vera-Rodriguez M,Rubio C

    更新日期:2017-05-01 00:00:00

  • Why are multiple pregnancy rates and single embryo transfer rates so different globally, and what do we do about it?

    abstract::In the early years of in vitro fertilization, overall pregnancy rates were low, and it was considered necessary to transfer more than one embryo to increase the chances of pregnancy. It was not until advances in assisted reproductive technologies resulting in increased pregnancy rates that the concept of transferring ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Adamson GD,Norman RJ

    更新日期:2020-10-01 00:00:00

  • Triplet tubal pregnancy treated by outpatient laparoscopic salpingostomy.

    abstract::To our knowledge, this represents the first case of a laparoscopically treated triplet EP and the first time that a double EP in the same tube was treated conservatively (with preservation of the tube). Multiple EPs may be more common than currently thought, and our report offers an alternative explanation for at leas...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Frishman GN,Steinhoff MM,Luciano AA

    更新日期:1990-11-01 00:00:00

  • Comparison of levonorgestrel intrauterine system versus hysterectomy on efficacy and quality of life in patients with adenomyosis.

    abstract:OBJECTIVE:To compare the levonorgestrel intrauterine system (LNG-IUS) with hysterectomy in patients with adenomyosis and to study the effects of both treatments on quality of life (QOL). DESIGN:Prospective randomized clinical trial. SETTING:Women's health teaching and research hospital. PATIENT(S):Eighty-six patient...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,随机对照试验


    authors: Ozdegirmenci O,Kayikcioglu F,Akgul MA,Kaplan M,Karcaaltincaba M,Haberal A,Akyol M

    更新日期:2011-02-01 00:00:00

  • Predicting the histologic dating of an endometrial biopsy specimen with the use of Doppler ultrasonography and hormone measurements in patients undergoing spontaneous ovulatory cycles.

    abstract:OBJECTIVE:To examine the relation between uterine blood flow and endometrial thickness on transvaginal Doppler ultrasonography, serum E2 and progesterone levels, and the histologic dating of an endometrial biopsy specimen obtained in the midluteal phase of a spontaneous cycle. DESIGN:Prospective clinical study. SETTI...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Sterzik K,Abt M,Grab D,Schneider V,Strehler E

    更新日期:2000-01-01 00:00:00

  • Magnetic resonance imaging and serum CA-125 in evaluating patients with endometriomas prior to medical therapy.

    abstract:OBJECTIVE:To determine whether magnetic resonance imaging (MRI) and serum levels of CA-125 can be used to evaluate patients with endometriomas before initiating medical treatment. DESIGN:Comparative study before and after medical treatment. SETTING:Shimane Medical University Hospital, Izumo, Japan. PATIENTS:Eleven c...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章


    authors: Takahashi K,Okada S,Okada M,Kitao M,Kaji Y,Sugimura K

    更新日期:1996-02-01 00:00:00

  • Avoiding ovarian hyperstimulation syndrome with the use of gonadotropin-releasing hormone agonist trigger.

    abstract::Ovarian hyperstimulation syndrome (OHSS) is one of the most serious, and potentially lethal, complications of controlled ovarian stimulation (COS). Induction of final oocyte maturation with a bolus of gonadotropin-releasing hormone (GnRH) agonist (GnRHa), instead of the criterion standard hCG, in patients undergoing o...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Fatemi HM,Garcia-Velasco J

    更新日期:2015-04-01 00:00:00

  • Light microscopy as an aid in predicting ureaplasma infection in human semen.

    abstract::The potential role of Ureaplasma urealyticum in human infertility make it desirable to screen barren couples for this infection. Semen specimens from 96 consecutive patients were evaluated in our clinic. Microbiologic results were correlated with the percentage of coiled and fuzzy tails in seminal cytology. These feat...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章


    authors: Toth A,Swenson CE,O'Leary WM

    更新日期:1978-11-01 00:00:00

  • Immature spermatids are not prevalent in semen from men who are receiving androgen-based contraceptive regimens.

    abstract:OBJECTIVE:To determine whether immature spermatids increase in semen in response to hormonal contraceptive treatments. Such a finding would support the existence of a defect in spermiogenesis, which in turn may explain the reported variability in sperm output. DESIGN:Semen smears were obtained from healthy men undergo...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Zhengwei Y,Wreford NG,Bremner WJ,Matsumoto AM,Anawalt BA,McLachlan RI

    更新日期:1998-01-01 00:00:00

  • A simplified method for freezing and storage of human semen.

    abstract::The use of 10-cm-long plastic tuberculin syringes for freezing and storage of human semen is introduced. This new method reduces the number of prefreezing and postthawing manipulations to a minimum. Postthawing motility, penetration of spermatozoa into cervical mucus, and results of the hamster ovum test were similar ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Kremer J,Dijkhuis JR,Jager S

    更新日期:1987-05-01 00:00:00

  • Increased expression of endothelin-1 and its receptors in varicocele: an immunohistochemical study.

    abstract::We hypothesized that diminished endothelin 1 (ET-1) expression at the spermatic vein wall level might be responsible for the development of varicocele. However, immunohistochemical evaluation of spermatic and control vein samples from 55 patients with varicocele showed overexpression of ET-1 and its receptors ETA and ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Gyftopoulos K,Chondrogianni C,Papadaki H

    更新日期:2011-06-30 00:00:00

  • Comparison of microdissection testicular sperm extraction, conventional testicular sperm extraction, and testicular sperm aspiration for nonobstructive azoospermia: a systematic review and meta-analysis.

    abstract:OBJECTIVE:To investigate the relative differences in outcomes among microdissection testicular sperm extraction (micro-TESE), conventional testicular sperm extraction (cTESE), and testicular sperm aspiration (TESA) in men with nonobstructive azoospermia. DESIGN:Systematic review and meta-analysis. SETTING:Outpatient ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,meta分析,评审


    authors: Bernie AM,Mata DA,Ramasamy R,Schlegel PN

    更新日期:2015-11-01 00:00:00

  • Serum concentrations of interleukin-2R (IL-2R), IL-6, IL-8, and tumor necrosis factor alpha in patients with ectopic pregnancy.

    abstract:OBJECTIVE:To investigate the diagnostic relevance of serum cytokine concentrations in ectopic pregnancy (EP). DESIGN:Cohort study. SETTING:University hospital. PATIENT(S):Seventeen women with EP, 22 women with miscarriage, and 33 women with normal intrauterine pregnancy, at comparable stages of gestation. INTERVENT...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Soriano D,Hugol D,Quang NT,Darai E

    更新日期:2003-04-01 00:00:00

  • Impact of infertility treatments on the health of newborns.

    abstract:OBJECTIVE:To measure the use of infertility treatments in the general population and their association with neonatal health. DESIGN:Cross-sectional, population-based study. SETTING:Canton of Vaud, Switzerland; Etude du Developpement des Nouveau-nés (EDEN), a prospective study of chronic childhood conditions. PATIENT...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Addor V,Santos-Eggimann B,Fawer CL,Paccaud F,Calame A

    更新日期:1998-02-01 00:00:00

  • Hormonal changes induced by short-term administration of gonadotropin-releasing hormone agonist during ovarian hyperstimulation for in vitro fertilization and their consequences for embryo development.

    abstract::Several regimens have been developed to administer gonadotropin-releasing hormone agonists in association with human menopausal gonadotropins (hMG) during follicular growth stimulation for in vitro fertilization. The aim of this study was to characterize hormonal changes induced by short-term administration of agonist...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Loumaye E,Vankrieken L,Depreester S,Psalti I,de Cooman S,Thomas K

    更新日期:1989-01-01 00:00:00

  • Impact of right-left differences in ovarian morphology on the ultrasound diagnosis of polycystic ovary syndrome.

    abstract:OBJECTIVE:To assess right-left differences in ultrasonographic markers of ovarian morphology and determine the impact on the diagnosis of polycystic ovarian morphology (PCOM). DESIGN:A cross-sectional study of data collected from 2006 to 2018. SETTING:Academic clinical research centers. PATIENT(S):Women with polycys...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,多中心研究


    authors: Jarrett BY,Vanden Brink H,Brooks ED,Hoeger KM,Spandorfer SD,Pierson RA,Chizen DR,Lujan ME

    更新日期:2019-11-01 00:00:00

  • Society for assisted reproductive technology position statement on donor suitability of recipients of smallpox vaccine (vaccinia virus).

    abstract::Although there is presently no definitive evidence linking vaccinia virus transmission through reproductive cells, SART/ASRM accordingly recommends that assisted reproductive technology (ART) practitioners consider deferring donors who have recently received smallpox vaccine or contracted symptomatic vaccinia virus in...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Practice Committee of the American Society for Reproductive Medicine.,Society for Assisted Reproductive Technology.

    更新日期:2004-04-01 00:00:00

  • Impact of obesity on women's health.

    abstract:OBJECTIVE:To review the impacts of obesity on women's reproductive health and fertility. DESIGN:Literature review. SETTING:Academic medical center. PATIENT(S):Forty articles are referenced. The number of patients evaluated in each of the studies varies from 33 to 213,208. INTERVENTION(S):Articles were identified fr...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Lash MM,Armstrong A

    更新日期:2009-05-01 00:00:00

  • Protein O-carboxylmethyltransferase in spermatozoa from normal and infertile men.

    abstract::In confirmation of several reports, suspensions of normal washed human spermatozoa exposed to a nonionic detergent exhibited considerable activity of the enzyme protein O-carboxylmethyltransferase (PCM), which catalyzes the methyl esterification of carboxyl groups of dicarboxylic amino acid residues in proteins. Vario...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Hatch R,Harvey SE,Williams-Ashman HG

    更新日期:1985-04-01 00:00:00

  • Comparison of techniques for the selection of bacteria-free sperm preparations.

    abstract::The authors compared the three most commonly used sperm preparation techniques--swim-up, fall-down, and Percoll gradient--for their ability to recover highly motile sperm and minimize bacterial contamination. Eleven human semen samples collected by masturbation were used and run in parallel with the three methods. A s...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Sun LS,Gastaldi C,Peterson EM,de la Maza LM,Stone SC

    更新日期:1987-10-01 00:00:00

  • Sequential analysis of spontaneous abortion. II. Collaborative study data show that gravidity determines a very substantial rise in risk.

    abstract::Evidence from more than 14,000 reproductive histories in the Collaborative Perinatal Project of the National Institute of Neurological and Communicative Disorders and Stroke indicates that the risk of spontaneous abortion increases considerably with gravidity. Maternal age, memory errors, and sampling bias related to ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Naylor AF,Warburton D

    更新日期:1979-03-01 00:00:00

  • Criteria, prevalence, and phenotypes of polycystic ovary syndrome.

    abstract::Polycystic ovary syndrome (PCOS) is a highly prevalent disorder effecting reproductive-aged women worldwide. This article addresses the evolution of the criteria used to diagnosis PCOS; reviews recent advances in the phenotypic approach, specifically in the context of the extended Rotterdam criteria; discusses limitat...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Lizneva D,Suturina L,Walker W,Brakta S,Gavrilova-Jordan L,Azziz R

    更新日期:2016-07-01 00:00:00

  • Isolation of a factor from normal human semen that accelerates dissolution of abnormally liquefying semen.

    abstract::A liquefraction factor from normal human seminal plasma was purified by cation and anion exchange chromatography. It is nondialyzable, heat-labile, and has many properties in common with the seminal protease "chymotrypsin-like enzyme." Its lytic effect is most distinctly observed in abnormal semen that is semisolid or...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Syner FN,Moghissi KS,Yanez J

    更新日期:1975-11-01 00:00:00

  • Expression of integrin messenger ribonucleic acid in human endometrium: a quantitative reverse transcription polymerase chain reaction study.

    abstract:OBJECTIVE:To assess the expression of selected integrin subunit's messenger RNA (mRNA) in human endometrium during the menstrual cycle. DESIGN:A prospective comparative study. SETTING:Academic research environment. PATIENT(S):Premenopausal women with histologically normal endometrium who were undergoing hysterectomy...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Dou Q,Williams RS,Chegini N

    更新日期:1999-02-01 00:00:00

  • Cryostorage of reproductive tissues in the in vitro fertilization laboratory: a committee opinion.

    abstract::This document is designed to assist in vitro fertility clinics in the management of cryopreserved reproductive tissues stored in cryogenic storage (cryostorage) tanks, based upon scientific principles and laboratory experience related to best practice for safe and reliable storage of cryopreserved reproductive tissue....

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Practice Committees of the American Society for Reproductive Medicine, Society for Reproductive Biologists and Technologists, and Society for Assisted Reproductive Technology. Electronic address:

    更新日期:2020-09-01 00:00:00

  • The effect of legislation on outcomes of assisted reproduction technology: lessons from the 2004 Italian law.

    abstract:OBJECTIVE:To evaluate the effect of the 2004 Italian regulations (insemination of

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: La Sala GB,Villani MT,Nicoli A,Valli B,Iannotti F,Blickstein I

    更新日期:2008-04-01 00:00:00

  • Quantification of mitochondrial DNA using real-time polymerase chain reaction in patients with premature ovarian failure.

    abstract:OBJECTIVE:To quantify mitochondrial DNA using real-time PCR in women with premature ovarian failure (POF) and a control group. DESIGN:Prospective study. SETTING:Genome Research Center for Reproductive Medicine and Infertility, Korea Ministry of Health & Welfare. PATIENT(S):Thrity patients with POF and 30 control ind...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章


    authors: Cha KY,Lee SH,Chung HM,Baek KH,Cho SW,Kwack KB

    更新日期:2005-12-01 00:00:00

  • Evaluation of ribonucleic acid amplification protocols for human oocyte transcriptome analysis.

    abstract:OBJECTIVE:To develop a reliable, reproducible, and sensitive method for investigating gene-expression profiles from individual human oocytes. DESIGN:Five commercially available protocols were investigated for their efficiency to amplify messenger RNA (mRNA) from 54 single human oocytes. Protocols resulting in sufficie...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Mantikou E,Bruning O,Mastenbroek S,Repping S,Breit TM,de Jong M

    更新日期:2016-02-01 00:00:00

  • Egg transport in the rabbit oviduct following ampullary resection and microsurgical end-to-end anastomosis.

    abstract::Surgical alteration of rabbit oviducts was employed to investigate the mechanisms of ovum transport. Resection of a 5- to 10-mm segment of midampulla was followed by microsurgical end-to-end anastomosis. One to two months later, direct observations were made, in situ, of ampullary egg transport. Stained cumulus masses...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Halbert SA,Patton DL

    更新日期:1979-12-01 00:00:00

  • Blood glucose and plasma insulin studies during three months' use of a low-estrogen type oral contraceptive.

    abstract::Blood glucose and plasma insulin levels were measured during an oral glucose tolerance test in 18 women before and after 3 months' use of a low-estrogen oral contraceptive containing 0.035 mg of ethinylestradiol and 0.5 mg of norethindrone. There was no significant change in either of these two metabolic parameters. I...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Spellacy WN,Buhi WC,Birk SA,Buggie J

    更新日期:1981-07-01 00:00:00