Abstract：
OBJECTIVE:Since little is known about the actual incidence and fate of vacuoles at different stages of development this preliminary study was set up to accurately measure vacuoles and track them to day 5. DESIGN:Prospective study. SETTING:Women's General Hospital in Austria. PATIENT(S):A total of 223 consecutive IVF and intracytoplasmic sperm injection (ICSI) cycles (206 patients). INTERVENTION(S):Accurate measurement of vacuoles. Affected gametes and embryos were cultured individually and tracked until day 5. MAIN OUTCOME MEASURE(S):Size and number of vacuoles, fertilization rate, blastocyst formation rate, blastocyst quality. RESULT(S):There was a significant relationship between size of the vacuole (cut-off value 14 microm) and fertilization (P<.05). At zygote stage the incidence of vacuoles was higher (P<.01) in ICSI (11.6%) than in IVF (5.3%). Only 32.2% of affected ICSI-embryos reached blastocyst stage on day 5 compared with 53.0% of the normal ones (P<.001). In terms of blastocyst formation vacuolization on day 4 (P<.001) turned out to be the most severe one. At blastocyst stage inner cell mass was affected less frequently than the trophectoderm (P<.05). CONCLUSION(S):Three types of vacuoles could be identified: (1) those already present at oocyte collection, which develop during maturation (day 0); (2) those artificially created by ICSI (day 1); and (3) those accompanied with developmental arrest (day 4). The later that vacuoles arose, the more detrimental their effect on blastocyst formation.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Ebner T,Moser M,Sommergruber M,Gaiswinkler U,Shebl O,Jesacher K,Tews Gdoi
10.1016/j.fertnstert.2005.02.009keywords:
subject
Has Abstractpub_date
2005-06-01 00:00:00pages
1635-40issue
6eissn
0015-0282issn
1556-5653pii
S0015-0282(05)00400-0journal_volume
83pub_type
杂志文章abstract：OBJECTIVE:To find alternative cryopreservation methods to improve the post-thaw fertilizing capacity of poor quality human sperm. DESIGN:Controlled clinical study. SETTING:Fertility clinic of a teaching hospital. PATIENTS:Men with poor quality semen samples, i.e., asthenozoospermia (< 40% motile sperm) and/or oligoz...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/s0015-0282(16)56227-x
更新日期：1993-10-01 00:00:00
abstract：:Based on American Society for Reproductive Medicine (ASRM) and Society for Assisted Reproductive Technology data available for 2010, ASRM's guidelines for the number of embryos to be transferred in in vitro fertilization cycles have been further refined in continuing efforts to reduce the number of higher-order multip...
journal_title：Fertility and sterility
pub_type： 杂志文章,实务指引
doi：10.1016/j.fertnstert.2012.09.038
更新日期：2013-01-01 00:00:00
abstract：OBJECTIVE:To compare and quantify leukocyte populations at the tubal implantation site, in the tubal mucosa away from the implantation site and in the intrauterine decidua in women who have had an ectopic tubal pregnancy with leukocyte populations in the decidualized endometrium in women who have had a normal intrauter...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(98)00005-3
更新日期：1998-04-01 00:00:00
abstract：OBJECTIVE:To compare the outcomes of intracytoplasmic sperm injection (ICSI) and in vitro fertilization (IVF) with fresh and cryopreserved testicular spermatozoa in patients with obstructive and nonobstructive azoospermia. DESIGN:Retrospective analysis of consecutive ICSI cycles. SETTING:Large urban reproductive medi...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(00)00416-7
更新日期：2000-05-01 00:00:00
abstract：OBJECTIVE:To report the case of a female who presented in childhood with symptoms and signs of hyperandrogenism secondary to an extraovarian steroid cell tumor. DESIGN:Case report. SETTING:Endocrine investigation unit of a university teaching hospital. PATIENT(S):An 11-year-old female presented with symptoms and sig...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2005.06.055
更新日期：2006-01-01 00:00:00
abstract：OBJECTIVE:To study whether preconceptual thyroid-stimulating hormone (TSH) and antithyroid peroxidase (TPO) antibodies are associated with poor reproductive outcomes in infertile women. DESIGN:Secondary analysis of data from two multicenter, randomized, controlled trials conducted by the Reproductive Medicine Network ...
journal_title：Fertility and sterility
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doi：10.1016/j.fertnstert.2017.08.026
更新日期：2017-10-25 00:00:00
abstract：OBJECTIVE:To determine if ultrastructural tissue trauma occurs in the junctional zone in uteri in adenomyosis. DESIGN:A case-control experimental study. SETTING:Endometriosis research center. PATIENT(S):Twelve uteri with adenomyosis, and 9 uteri without adenomyosis, were gained during laparoscopy-assisted vaginal hy...
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pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.09.002
更新日期：2015-12-01 00:00:00
abstract：:Where histology used the presence of glands and/or stroma in the myometrium as pathognomonic for adenomyosis, imaging uses the appearance of the myometrium, the presence of striations, related to the presence of endometrial tissue within the myometrium, the presence of intramyometrial cystic structures and the size an...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
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更新日期：2018-03-01 00:00:00
abstract：:We describe a cotyledonoid dissecting leiomyoma in a 33-year-old nulliparous woman treated by laparoscopy. ...
journal_title：Fertility and sterility
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doi：10.1016/j.fertnstert.2008.08.131
更新日期：2009-04-01 00:00:00
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journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.07.1159
更新日期：2015-11-01 00:00:00
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doi：10.1016/j.fertnstert.2008.12.096
更新日期：2010-03-15 00:00:00
abstract：OBJECTIVE:To investigate the success rate of proximal tubal occlusion with Essure devices in subfertile women with hydrosalpinges, and to observe the results of subsequent treatment with IVF. DESIGN:Prospective, single-arm, clinical study. SETTING:University hospital and teaching hospital. PATIENT(S):Ten women with ...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章
doi：10.1016/j.fertnstert.2008.11.022
更新日期：2010-03-01 00:00:00
abstract：:Patient requests for transfer of embryos with genetic anomalies linked to serious health-affecting disorders detected in preimplantation testing are rare but do exist. This Opinion sets out the possible rationales for a provider's decision to assist or decline to assist in such transfers. The Committee concludes in mo...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2017.02.121
更新日期：2017-05-01 00:00:00
abstract：:Cryopreservation has become a central pillar in assisted reproduction, reflected in the exponential increase of "freeze all" cycles in the past few years. Vitrification makes it possible to cool and warm human eggs and embryos with far less cryo-damage than 'slow-freeze' and allows nearly intact survival of embryos wi...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
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更新日期：2020-02-01 00:00:00
abstract：OBJECTIVES:To investigate the presence of Poly (ADP-ribose) polymerase (PARP) and evaluate its function in ejaculated spermatozoa. DESIGN:Experimental study. SETTING:Reproductive Research Center. PATIENT(S):Semen specimens from 18 healthy donors and 12 infertile males. INTERVENTION(S):Ejaculated spermatozoa were su...
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pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.12.079
更新日期：2009-03-01 00:00:00
abstract：:Semen samples from 35 fertile men and husbands of 32 infertile couples were evaluated with the use of a rapid computerized sperm motion analysis (CSA). The counts and percentages of motile sperm measured by routine semen analysis (RSA) and CSA were comparable in both groups. In CSA, mean values of swimming speed (micr...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1986-09-01 00:00:00
abstract：:Menopausal hormone therapy (HT) has complex biologic effects but continues to have an important clinical role in the management of vasomotor and other menopausal symptoms. The rational use of menopausal HT requires balancing the potential benefits and risks of treatment. Findings from the Women's Health Initiative (WH...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
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更新日期：2014-04-01 00:00:00
abstract：OBJECTIVE:To examine the emotional, sexual, physical, and quality-of-life (QOL) impact on infertile women awaiting oocyte donation. DESIGN:Cross-sectional cohort study. SETTING:Cancer center and reproductive medicine center. PATIENT(S):Fifty infertile women awaiting oocyte donation, enrolled from October 2006 to Feb...
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doi：10.1016/j.fertnstert.2010.10.004
更新日期：2011-02-01 00:00:00
abstract：OBJECTIVE:To compare buoyant density and sedimentation kinetics of human immunodeficiency virus 1 (HIV-1) in two sperm-washing media, Percoll and Pureception. DESIGN:Laboratory study. SETTING:University hospital. PATIENT(S):None. INTERVENTION(S):Buoyant density and sedimentation kinetics of HIV-1 particles (MOLT-4/...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.09.025
更新日期：2008-11-01 00:00:00
abstract：:The distribution of genotype frequencies, allele frequencies, and haplotype frequencies of variable number of tandem repeats (VNTR) in interleukin (IL) 1RN and IL-1beta (-511C > T, exon-5C.>T) polymorphism demonstrated a positive association with the occurrence of polycystic ovary syndrome (PCOS). The carriage of alle...
journal_title：Fertility and sterility
pub_type： 信件
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更新日期：2010-06-01 00:00:00
abstract：OBJECTIVE:To study the association between low percentages of intact sperm acrosomes and fertilization failures in conventional IVF procedures. DESIGN:A retrospective study. SETTING:Clinical and academic research environment. PATIENT(S):Patients undergoing treatment of infertility. INTERVENTION(S):Sperm cells were ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(99)00201-0
更新日期：1999-07-01 00:00:00
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journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)57179-9
更新日期：1994-12-01 00:00:00
abstract：OBJECTIVE:Immunobead testing (IBT), the current standard for antisperm antibody detection, is time consuming and somewhat subjective. To overcome these limitations and maintain accuracy, we studied an immunofluorescent assay using flow cytometry. DESIGN:A validation study comparing flow cytometry to IBT in the detecti...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：OBJECTIVE:To examine the small antral follicle (SAF) cohort in ovaries of adult rhesus monkeys after consumption of a Western-style diet (WSD), with or without chronically elevated androgen levels since before puberty. DESIGN:Cholesterol or T (n = 6 per group) implants were placed SC in female rhesus macaques beginnin...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.11.045
更新日期：2016-04-01 00:00:00
abstract：OBJECTIVE:To determine the levels of postacrosomal WW binding protein (PAWP) in the spermatozoa of men that were used clinically for intracytoplasmic sperm injection (ICSI) and to correlate them with infertility treatment outcomes. DESIGN:Prospective clinical and laboratory study. SETTING:University-based laboratory ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.05.003
更新日期：2014-08-01 00:00:00
abstract：:The effects of maternal preovulatory serum and human fetal cord serum supplement in culture medium in human in vitro fertilization and embryo development were compared in 208 cases over an 8-month period when there were no significant changes in other variables. A significantly higher pregnancy rate was observed in th...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)47537-0
更新日期：1984-01-01 00:00:00
abstract：OBJECTIVE:To investigate the relationship between body mass index and intercourse compliance in the Reproductive Medicine Network's Pregnancy in Polycystic Ovary Syndrome (RMN PPCOS) Trial. DESIGN:Post hoc data analysis of subjects in the RMN PPCOS Trial. SETTING:Academic medical centers. INTERVENTION(S):None. PATI...
journal_title：Fertility and sterility
pub_type： 杂志文章,多中心研究
doi：10.1016/j.fertnstert.2009.05.048
更新日期：2010-09-01 00:00:00
abstract：:During hysteroscopy the uterus may be distended with carbon dioxide (CO2), nitrous oxide (N2O), or Hyskon (a high molecular weight dextran). An initial study in 27 patients (group 1) using arterialized venous blood samples demonstrated rises in carbon dioxide tension (PCO2) when N2O was insufflated by using a laparosc...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)44179-8
更新日期：1979-08-01 00:00:00
abstract：OBJECTIVE:To examine the expression and localization of Ets-1 in the rat ovary during the estrous cycle and pregnancy, and to investigate its effects on ovarian function. DESIGN:Prospective, randomized study. SETTING:Department of Physiology at Harbin Medical University. ANIMAL(S):Pubertal female Wistar rats. INTER...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.02.166
更新日期：2009-05-01 00:00:00
abstract：OBJECTIVE:To develop and validate a specific simple measure of insulin sensitivity using oral glucose tolerance test (OGTT) values for lean polycystic ovary syndrome (PCOS) women. DESIGN:Retrospective study. SETTING:Gynecologic Outpatient Clinic of University Hospital, affiliated with Unit of Gynecologic Endocrinolog...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.04.004
更新日期：2014-07-01 00:00:00
