Sperm content of postacrosomal WW binding protein is related to fertilization outcomes in patients undergoing assisted reproductive technology.


OBJECTIVE:To determine the levels of postacrosomal WW binding protein (PAWP) in the spermatozoa of men that were used clinically for intracytoplasmic sperm injection (ICSI) and to correlate them with infertility treatment outcomes. DESIGN:Prospective clinical and laboratory study. SETTING:University-based laboratory and infertility clinic. PATIENT(S):Men undergoing ICSI for the treatment of couples' infertility (n=110). INTERVENTION(S):Quantitative analysis of sperm PAWP levels by flow cytometry and developmental analysis of PAWP expression by immunoblotting, immunofluorescence, and immunohistochemistry. MAIN OUTCOME MEASURE(S):PAWP flow-cytometric levels and immunolocalization in spermatozoa. RESULT(S):A strong positive correlation was found between PAWP expression levels and fertilization rates after ICSI, with high levels of PAWP being associated with higher fertilization rates; the positive correlation was independent of age, DNA fragmentation index, and other sperm parameters. PAWP expression levels were correlated with embryonic development, with high levels of PAWP being associated with a lower number of arrested embryos within 3-5 days post-ICSI. PAWP expression was detected during the late stages of human spermiogenesis in elongating spermatids, confirming previous findings in various animal models. CONCLUSION(S):Our clinical data from infertile couples demonstrate significant correlations between sperm PAWP levels and both fertilization rates and normal embryonic development after ICSI. Considering its proposed role in the initiation of oocyte activation, we suggest that PAWP could have potential applications in the diagnosis and treatment of infertility.


Fertil Steril


Fertility and sterility


Aarabi M,Balakier H,Bashar S,Moskovtsev SI,Sutovsky P,Librach CL,Oko R




Has Abstract


2014-08-01 00:00:00














  • Treatment of hirsutism: comparisons between different antiandrogens with central and peripheral effects.

    abstract:OBJECTIVE:To compare the clinical and endocrinologic effects of cyproterone acetate (CPA), an antiandrogen with progestational activity; flutamide, a nonsteroidal antiandrogen, and finasteride, an inhibitor of 5alpha-reductase. DESIGN:Randomized, open, controlled clinical study. SETTING:Department of Obstetrics and G...

    journal_title:Fertility and sterility

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Fruzzetti F,Bersi C,Parrini D,Ricci C,Genazzani AR

    更新日期:1999-03-01 00:00:00

  • Male immunologic infertility: sperm performance on in vitro fertilization.

    abstract:OBJECTIVE:To analyze sperm performance in a group of patients with male immunologic infertility treated with IVF-ET. DESIGN:Retrospective clinical study. SETTING:Patients attending a private IVF clinic. PATIENT(S):The study group comprised seven men with significant levels of surface-bound antisperm antibodies treat...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Vazquez-Levin MH,Notrica JA,Polak de Fried E

    更新日期:1997-10-01 00:00:00

  • Comparison of levonorgestrel intrauterine system versus hysterectomy on efficacy and quality of life in patients with adenomyosis.

    abstract:OBJECTIVE:To compare the levonorgestrel intrauterine system (LNG-IUS) with hysterectomy in patients with adenomyosis and to study the effects of both treatments on quality of life (QOL). DESIGN:Prospective randomized clinical trial. SETTING:Women's health teaching and research hospital. PATIENT(S):Eighty-six patient...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,随机对照试验


    authors: Ozdegirmenci O,Kayikcioglu F,Akgul MA,Kaplan M,Karcaaltincaba M,Haberal A,Akyol M

    更新日期:2011-02-01 00:00:00

  • Recruiting egg donors online: an analysis of in vitro fertilization clinic and agency websites' adherence to American Society for Reproductive Medicine guidelines.

    abstract:OBJECTIVE:To examine compliance with ethical guidelines of the American Society for Reproductive Medicine (ASRM) regarding trait-based payment variation, presentation of risks, and minimum recruitment age. DESIGN:In June 2010, we systematically examined 207 websites, of which 102 were egg donor agency or IVF clinic we...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Keehn J,Holwell E,Abdul-Karim R,Chin LJ,Leu CS,Sauer MV,Klitzman R

    更新日期:2012-10-01 00:00:00

  • The accuracy of serum chlamydial antibodies in the diagnosis of tubal pathology: a meta-analysis.

    abstract:OBJECTIVE:To assess the discriminative capacity of Chlamydia antibody titers in the diagnosis of tubed pathology in subfertile patients. DESIGN:Meta-analysis of studies comparing Chlamydia antibody titers and laparoscopy for tubal patency and peritubal adhesions. PATIENTS:A total of 2,729 patients with subfertility i...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,meta分析


    authors: Mol BW,Dijkman B,Wertheim P,Lijmer J,van der Veen F,Bossuyt PM

    更新日期:1997-06-01 00:00:00

  • Klinefelter's syndrome with unilateral absence of vas deferens.

    abstract:OBJECTIVE:To report a case of Klinefelter's syndrome with unilateral absence of vas deferens. DESIGN:Case report. SETTING:Tertiary-care infertility clinic. PATIENT(S):A 28-year-old man with the complaint of infertility. INTERVENTION(S):None. MAIN OUTCOME MEASURE(S):Physical examination, genetic and hormonal evalua...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Baydilli N,Gökçe A,Karabulut SY,Ekmekcioglu O

    更新日期:2010-09-01 00:00:00

  • Artificial insemination using homologous semen: a review of 158 cases.

    abstract::One hundred fifty-eight women underwent artificial insemination with homologous semen (AIH) in an attempt to achieve conception. Only 15 (9.5%) were successful. Women with anatomical abnormalities were not excluded from the study and they were less successful than the normal women, but results were disappointing in bo...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Dixon RE,Buttram VC Jr,Schum CW

    更新日期:1976-06-01 00:00:00

  • Increases in progesterone after human chorionic gonadotropin administration may predict cycle outcome in patients undergoing in vitro fertilization-embryo transfer.

    abstract::In this study, both IVF-ET cycle and pregnancy outcome were compared with increases seen in P concentration during the 24-hour period from 12 hours before to 12 hours after hCG administration. A significantly higher PR (P < 0.001) was seen in women who had at least a threefold increase in P during this time period. Fu...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Prien SD,Canez MS,Messer RH

    更新日期:1994-11-01 00:00:00

  • A meta-analysis of ultrasound-guided versus clinical touch embryo transfer.

    abstract:OBJECTIVE:To determine the relative efficacy of ultrasound-guided embryo transfer and embryo transfer by clinical touch alone. DESIGN:Systematic review and meta-analysis of randomized, controlled trials comparing ultrasound-guided embryo transfer with embryo transfer by clinical touch alone. SETTING:Infertility cente...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,meta分析,评审


    authors: Buckett WM

    更新日期:2003-10-01 00:00:00

  • Raw and test-thaw semen parameters after cryopreservation among men with newly diagnosed cancer.

    abstract:OBJECTIVE:To characterize sperm parameters from thawed semen samples of men with different cancers who cryopreserved semen before oncologic therapy. DESIGN:Retrospective cohort study. SETTING:Tertiary academic medical center. PATIENT(S):1,010 semen samples collected between 1994 to 2010. INTERVENTION(S):None. MAIN...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Hotaling JM,Lopushnyan NA,Davenport M,Christensen H,Pagel ER,Muller CH,Walsh TJ

    更新日期:2013-02-01 00:00:00

  • Effect of endometriosis on in vitro fertilization.

    abstract:OBJECTIVE:To investigate the IVF outcome for patients with endometriosis. DESIGN:Meta-analysis. SETTING:Academic research center. PATIENT(S):A MEDLINE search and review of the literature were performed. Patients were classified by level of endometriosis, and controls were classified according to the indication for I...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,meta分析


    authors: Barnhart K,Dunsmoor-Su R,Coutifaris C

    更新日期:2002-06-01 00:00:00

  • Salpingitis isthmica nodosa: a review of the literature, discussion of clinical significance, and consideration of patient management.

    abstract:OBJECTIVES:To examine and discuss the pathology, diagnosis, incidence, and patient profile of salpingitis isthmica nodosa and to question its natural history, propose management strategies, and identify areas of promising research. DESIGN:Over 50 studies were reviewed, evaluated, and compared to offer the clinician a ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Jenkins CS,Williams SR,Schmidt GE

    更新日期:1993-10-01 00:00:00

  • Ovulation induction in women with premature ovarian failure: a prospective, crossover study.

    abstract::A prospective crossover study comparing ovulation induction techniques in eight women with premature ovarian failure is presented. These patients were treated with FSH rebound techniques using the GnRH-a, LA, alone in one treatment cycle and the same plus menopausal gonadotropins in the other treatment cycle. Two wome...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Rosen GF,Stone SC,Yee B

    更新日期:1992-02-01 00:00:00

  • Effect of supraphysiologic doses of testosterone on fasting plasma total homocysteine concentrations in men with Klinefelter's syndrome.

    abstract:OBJECTIVE:To examine the effect of supraphysiologic doses of testosterone (T) on plasma total homocysteine (tHcy) concentrations in patients with Klinefelter's syndrome (KS). DESIGN:Prospective clinical study. SETTING:University hospital. PATIENT(S):Thirty-two newly diagnosed male patients with KS, and 20 healthy, v...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Yesilova Z,Ozata M,Oktenli C,Sanisoglu SY,Erbil MK,Dagalp K

    更新日期:2004-05-01 00:00:00

  • The effect of three hormone replacement regimens on bone density in the aged ovariectomized rat.

    abstract:OBJECTIVE:To investigate the effects of estrogen (E) alone or with cyclic, continuous, or interrupted P regimens on bone density in an aged rat model. DESIGN:Randomized controlled trial. ANIMALS:Twenty 12-month-old female Sprague-Dawley rats were sham operated (intact control) and 100 were ovariectomized (OVX). INTE...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Vanin CM,MacLusky NJ,Grynpas MD,Casper RF

    更新日期:1995-03-01 00:00:00

  • Prevalence, consequence, and significance of reverse cleavage by human embryos viewed with the use of the Embryoscope time-lapse video system.

    abstract:OBJECTIVE:To investigate the prevalence and potential causes of reverse cleavage (RC) by human early-cleavage embryos and its associations with embryonic development and implantation after transfer. DESIGN:Clinical retrospective cohort study. SETTING:Private fertility treatment center. PATIENT(S):A total of 126 cons...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Liu Y,Chapple V,Roberts P,Matson P

    更新日期:2014-11-01 00:00:00

  • Enrichment of spermatogonial stem cells from long-term cultured human testicular cells.

    abstract:OBJECTIVE:To evaluate the degree of enrichment of spermatogonial stem cells (SSCs) from human testicular cell cultures by ITGA6+, HLA-/ITGA6+, GPR125+, and HLA-/GPR125+ magnetic-assisted cell sorting (MACS). DESIGN:Experimental basic science study. SETTING:Reproductive biology laboratory. PATIENT(S):Multiple samples...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Nickkholgh B,Mizrak SC,Korver CM,van Daalen SK,Meissner A,Repping S,van Pelt AM

    更新日期:2014-08-01 00:00:00

  • Recombinant human leukemia inhibitory factor does not enhance in vitro human blastocyst formation.

    abstract:OBJECTIVE:To assess the effect of human recombinant leukemia inhibitory factor in different doses on human blastocyst formation. SETTING:A university-based tertiary referral center (The Toronto Hospital). INTERVENTIONS:Nontransferable human embryos (n = 473) at the two- to six-cell stage were obtained from patients u...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Jurisicova A,Ben-Chetrit A,Varmuza SL,Casper RF

    更新日期:1995-11-01 00:00:00

  • Leukemia inhibitory factor is dysregulated in the endometrium and uterine flushing fluid of patients with adenomyosis during implantation window.

    abstract:OBJECTIVE:To determine whether the expression of leukemia inhibitory factor during the implantation window in endometrial tissue and uterine flushing fluid of patients with adenomyosis differs from that of healthy fertile women. DESIGN:Experimental study using endometrial tissue and uterine flushing fluid. SETTING:Pe...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Xiao Y,Sun X,Yang X,Zhang J,Xue Q,Cai B,Zhou Y

    更新日期:2010-06-01 00:00:00

  • Relationship between genital tract infections, sperm antibodies in seminal fluid, and infertility.

    abstract::To explore possible relationships between genital tract infections, sperm antibodies, and infertility, seminal fluid from 100 men was analyzed. Sperm antibodies, measured by an enzyme-linked immunosorbent assay, were detected in 14 of 29 men (48%) with culture-positive asymptomatic infections, 8 of 17 men (47%) with a...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Witkin SS,Toth A

    更新日期:1983-12-01 00:00:00

  • Interruption of pregnancy by prostaglandin 15-methyl F2alpha.

    abstract::The current study was formulated to investigate the abortifacient activity of prostaglandin 15-methyl F2alpha (15-methyl PGF2alpha) administered intramuscularly to 80 healthy women with gestations between 8 and 22 weeks. Goals were the establishment of an effective dosage schedule and assessment of the incidence and s...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Bolognese RJ,Corson SL

    更新日期:1975-07-01 00:00:00

  • Transmyometrial embryo transfer after difficult immediate mock transcervical transfer.

    abstract:OBJECTIVE:To evaluate the place of ultrasound-directed transvaginal transmyometrial ET in a protocol using both the transcervical and transmyometrial routes in a step-wise fashion. DESIGN:A prospective descriptive clinical study. SETTING:A university-based assisted conception unit. PATIENTS:Thirteen patients who had...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Sharif K,Afnan M,Lenton W,Bilalis D,Hunjan M,Khalaf Y

    更新日期:1996-05-01 00:00:00

  • Immunolocalization of angiopoietin 1 in human peritoneal endometriotic lesions.

    abstract:OBJECTIVE:To analyze the expression of angiopoietin 1 (ANGPT1) in pigmented and nonpigmented endometriosis implants and specify its role in the interaction of angiogenic factors. DESIGN:Experimental retrospective study. SETTING:University hospital. PATIENT(S):Eighty-two sections from 201 laparoscopic tissue samples ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Gescher DM,Berndorff U,Meyhoefer-Malik A,Moubayed P,Malik E

    更新日期:2004-03-01 00:00:00

  • Ovarian torsion in a premenopausal woman treated with tamoxifen for breast cancer.

    abstract::Tamoxifen therapy in premenopausal women with breast cancer may be associated with the development of ovarian cysts. In this report, a case of ovarian torsion associated with TAM therapy is described. ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Barbieri RL,Ferracci AL,Droesch JN,Rochelson BL

    更新日期:1993-02-01 00:00:00

  • Importance of embryo transfer technique in maximizing assisted reproductive outcomes.

    abstract::Embryo transfer is arguably the most critical process in the sequential events that encompass an IVF cycle. Several variables play a role in the success of a transfer, including catheter type, atraumatic technique, and the use of ultrasound guidance. The inclusion of hyaluronan in the ET media also has a benefit for i...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Schoolcraft WB

    更新日期:2016-04-01 00:00:00

  • Characterization of aneuploidy rates, protamine levels, ultrastructure, and functional ability of round-headed sperm from two siblings and implications for intracytoplasmic sperm injection.

    abstract:OBJECTIVE:To characterize and contrast protamine levels, chromatin decondensation, chromosome aneuploidy rates, and functional ability of round-headed sperm from two brothers with round-headed sperm syndrome. DESIGN:Analysis of semen samples from siblings with round-headed sperm syndrome and comparison with semen of f...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Carrell DT,Emery BR,Liu L

    更新日期:1999-03-01 00:00:00

  • Effects of left varicocele ligation in subfertile males with absent or atrophic right testes.

    abstract::Fifteen subfertile males with absent or atrophic right testes underwent ligation of left varicoceles. Sperm concentration rose significantly with correction of scrotal thermographic abnormality and nine wives (60%) became pregnant. ...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Hendry WF

    更新日期:1992-06-01 00:00:00

  • Endometrial microbiota-new player in town.

    abstract::Detection of bacteria with molecular techniques has enabled the study of low biomass microbiomes in tissues and organs previously considered sterile, such as the endometrium. Subsequently, an abnormal endometrial microbiota has been associated with implantation failure, pregnancy loss, and other gynecological and obst...

    journal_title:Fertility and sterility

    pub_type: 杂志文章,评审


    authors: Moreno I,Franasiak JM

    更新日期:2017-07-01 00:00:00

  • Identification and treatment of men with phospholipase Cζ-defective spermatozoa.

    abstract:OBJECTIVE:To identify and treat the gamete responsible for complete fertilization failure with intracytoplasmic sperm injection (ICSI) using a newly proposed assisted gamete treatment (AGT). DESIGN:Prospective cohort study. SETTING:Center for reproductive medicine. PATIENT(S):One-hundred and fourteen couples with an...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Cheung S,Xie P,Parrella A,Keating D,Rosenwaks Z,Palermo GD

    更新日期:2020-09-01 00:00:00

  • A sonographic classification of adenomyosis: interobserver reproducibility in the evaluation of type and degree of the myometrial involvement.

    abstract:OBJECTIVE:To study the interobserver reproducibility of our new ultrasonographic mapping system to define the type and extension of uterine adenomyosis. DESIGN:Interobserver study involving two observers with different medical backgrounds and gynecological ultrasound experience. SETTING:University hospital. PATIENTS...

    journal_title:Fertility and sterility

    pub_type: 杂志文章


    authors: Lazzeri L,Morosetti G,Centini G,Monti G,Zupi E,Piccione E,Exacoustos C

    更新日期:2018-11-01 00:00:00