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Buoyant density and sedimentation dynamics of HIV-1 in two density-gradient media for semen processing.

Abstract：

OBJECTIVE:To compare buoyant density and sedimentation kinetics of human immunodeficiency virus 1 (HIV-1) in two sperm-washing media, Percoll and Pureception. DESIGN:Laboratory study. SETTING:University hospital. PATIENT(S):None. INTERVENTION(S):Buoyant density and sedimentation kinetics of HIV-1 particles (MOLT-4/LAI strain) were measured in Percoll and Pureception using isopyknic ultracentrifugation and continuous-density-gradient centrifugation. MAIN OUTCOME MEASURE(S):The HIV-1 particles were detected and semiquantified using a reverse transcription polymerase chain reaction (RT-PCR) for HIV-1 RNA. RESULT(S):Calculated buoyant density of HIV-1 was approximately 1.042 in both media in isopyknic centrifugation. However, most HIV-1 particles were found in fractions with specific gravity less than 1.04 in both media, even after 40 minutes of density-gradient centrifugation at 1,600 g. Small viral accumulations were observed at the bottom of the tube in Pureception density gradients. CONCLUSION(S):Although we found very high efficiency of HIV-1 removal using density-gradient centrifugation, a minute quantity of virus was found at the bottom of the gradient tube when Pureception was used as the medium.

journal_name

Fertil Steril

journal_title

Fertility and sterility

authors

Kuji N,Yoshii T,Hamatani T,Hanabusa H,Yoshimura Y,Kato S

doi

10.1016/j.fertnstert.2007.09.025

subject

Has Abstract

pub_date

2008-11-01 00:00:00

pages

1983-7

issue

5

eissn

0015-0282

issn

1556-5653

pii

S0015-0282(07)03647-3

journal_volume

90

pub_type

杂志文章

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