Abstract：
OBJECTIVES:To investigate the presence of Poly (ADP-ribose) polymerase (PARP) and evaluate its function in ejaculated spermatozoa. DESIGN:Experimental study. SETTING:Reproductive Research Center. PATIENT(S):Semen specimens from 18 healthy donors and 12 infertile males. INTERVENTION(S):Ejaculated spermatozoa were subjected to sperm fractionation with a double-layer density gradient, protein extraction, detection, immunoblotting, and mass spectrometry. Semen samples were exposed to PARP inducer staurosporine, or hydrogen peroxide with or without PARP inhibitor 3-aminobenzimide. Annexin V assay was examined for apoptosis by flow cytometry. RESULT(S):We detected approximately 75 kDa, approximately 63 kDa, and approximately 60 kDa PARP homologues on the immunoblot of mature and immature sperm fraction isolated from human ejaculate, which were identified as PARP-1 ( approximately 75 kDa), PARP-9 ( approximately 63 kDa), and PARP-2 ( approximately 60 kDa), respectively. Western blot analysis showed a positive correlation between the amount of PARP protein and sperm maturity. PARP proteins (75 kDa, 63 kDa, and 60 kDa) were evaluated after inducing apoptosis by hydrogen peroxide and staurosporine exposure. CONCLUSION(S):PARP-2 may have a role in prevention of oxygen species/oxidative stress and chemical (staurosporine)-induced sperm cell apoptosis. The presence of the PARP homologue, that is, PARP-1 ( approximately 75 kDa), suggests its role in preventing damage of mature sperm. Additional studies are needed to delineate the role of PARP-9 in sperm physiology. The results from our study indicate an active role for PARPs in sperm cell physiology in preventing apoptosis.
journal_name
Fertil Steriljournal_title
Fertility and sterilityauthors
Jha R,Agarwal A,Mahfouz R,Paasch U,Grunewald S,Sabanegh E,Yadav SP,Sharma Rdoi
10.1016/j.fertnstert.2007.12.079subject
Has Abstractpub_date
2009-03-01 00:00:00pages
782-90issue
3eissn
0015-0282issn
1556-5653pii
S0015-0282(08)00006-Xjournal_volume
91pub_type
杂志文章abstract：:The hyperandrogenic-insulin-resistant acanthosis nigricans syndrome affects between 2% and 5% of hirsute women and is characterized by INS resistance, elevated INS levels, acanthosis nigricans, and androgen excess. These patients' response to therapy is unclear, although long-acting GnRH-a suppression has been propose...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1994-03-01 00:00:00
abstract：:Cell-mediated immunity in rhesus monkeys that had been vasectomized for 2, 4, 7, or 11 years was measured by lymphocyte blastogenesis following stimulation with concanavalin A, phytohemagglutinin (PHA), and pokeweed mitogens. Several of the 7- and 11-year vasectomized animals had significantly reduced PHA reactivity w...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)42983-3
更新日期：1977-12-01 00:00:00
abstract：OBJECTIVE:To determine the aneuploidy rate in embryos of women with idiopathic recurrent miscarriages and to evaluate whether preimplantation genetic diagnosis for aneuploidy screening could be a feasible approach to improve the possibility of successful pregnancy in these couples. DESIGN:Prospective cohort study. SE...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.06.071
更新日期：2005-02-01 00:00:00
abstract：OBJECTIVE:To compare the performance of the SureView catheter, a new ultrasonic embryo transfer (ET), with the classic Wallace catheter during ultrasound-guided ET. DESIGN:Prospective, randomized study. SETTING:Private, academically affiliated clinic. PATIENT(S):175 patients undergoing donor egg in vitro fertilizati...
journal_title：Fertility and sterility
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.fertnstert.2009.01.090
更新日期：2010-02-01 00:00:00
abstract：OBJECTIVE:To introduce a technique for hysteroscopic resection of submucous myoma arising from the uterine fundus. DESIGN:Case report. SETTING:University hospital. PATIENT(S):A 43-year-old nulliparous Japanese woman. INTERVENTION(S):An original combination technique of hysteroresectoscopy using circumferential myom...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.03.066
更新日期：2006-11-01 00:00:00
abstract：OBJECTIVE:To determine the efficiency of oocyte retrieval (OR) timing based on the occurrence of spontaneous LH surge during natural cycle IVF (ncIVF) treatment. DESIGN:Retrospective cohort study. The cohort was divided into five subgroups according to the presumed stage of spontaneous LH surge on scheduling day (1A: ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2014.01.016
更新日期：2014-04-01 00:00:00
abstract：OBJECTIVE:To evaluate the influence of hormonal changes during the menstrual cycle on the mammary gland of female cynomolgus monkeys. DESIGN:Paired breast biopsy samples were obtained during the follicular and luteal phases of the cycle. Cycle characteristics were assessed by vaginal bleeding, serum hormones, vaginal ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2004.04.035
更新日期：2004-10-01 00:00:00
abstract：OBJECTIVE:To visualize by microhysteroscopy any possible lesions on the endocervix and endometrium made by the catheters commonly used for embryo transfer (ET). DESIGN:Prospective descriptive study. Tertiary fertility center (IFER). PATIENT(S):Twenty-three infertile patients underwent a mock transfer before a microhy...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(03)00607-1
更新日期：2003-08-01 00:00:00
abstract：:Polycystic ovary disease generally is associated with elevated androgen levels and elevated luteinizing hormone (LH) levels, whereas follicle-stimulating hormone (FSH) levels are (sub)normal. To assess the role of androgens on gonadotropin secretion, we investigated the effect of 6 weeks of testosterone (T) undecanoat...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)54194-6
更新日期：1991-03-01 00:00:00
abstract：OBJECTIVE:To evaluate the influence of somatostatin analogue (octreotide) in the function of hypothalamic-pituitary-adrenal (HPA) axis in women with polycystic ovary syndrome (PCOS). SETTING:Women referred to the Department of Obstetrics and Gynecology, Università Cattolica del Sacro Cuore. PATIENT(S):Twelve PCOS wom...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(97)81852-3
更新日期：1997-01-01 00:00:00
abstract：:The biochemical and histological features of two related patients with the complete form of the androgen insensitivity syndrome (AIS) coexisting with incomplete regression of the Müllerian ducts are described. Both patients presented unilateral Müllerian derivatives (fallopian tube) identified by microscopic examinati...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:Activities of deoxyribonucleic acid (DNA) polymerase alpha, beta, and gamma were measured in extracts of testicular biopsy specimen obtained from 37 cases of male infertility with left varicocele and compared with those of 6 normal controls. It was observed that levels of DNA polymerase alpha, beta, and gamma were sig...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)60318-7
更新日期：1988-11-01 00:00:00
abstract：INTRODUCTION:Polycystic ovary syndrome (PCOS) is a highly prevalent disorder among postmenarcheal premenopausal women, accounting for much of anovulatory infertility, and it is associated with several comorbidities. The natural history of PCOS, as well as hypotheses regarding its etiology, facilitates employment of the...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2005.09.066
更新日期：2006-07-01 00:00:00
abstract：:We present a case of combined intrauterine and cornual (interstitial) pregnancy after IVF-ET, with survival of the infant after the cornual pregnancy. The diagnosis of this rare phenomenon is difficult; a high index of suspicion and frequently ultrasound monitoring may enable early diagnosis in other forms of heteroto...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)53473-6
更新日期：1990-04-01 00:00:00
abstract：OBJECTIVE:Our objective is to report observed changes in thyroid-stimulating hormone (TSH) in two patients undergoing super-ovulation for IVF. DESIGN:Case report. SETTING:Private assisted reproduction practices. PATIENT(S):Two hypothyroid women taking thyroxine replacement therapy undergoing super-ovulation for IVF....
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2009.11.051
更新日期：2010-05-01 00:00:00
abstract：OBJECTIVE:To evaluate the effects of cryopreservation on the survival, cleavage, and morphology of embryos and on the implantation and embryonic loss rates of human zygotes obtained after ICSI compared with frozen-thawed zygotes obtained after traditional IVF. A further objective was to evaluate the same parameters in ...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(98)00021-1
更新日期：1998-04-01 00:00:00
abstract：:This opinion addresses the ethics of providing fertility treatment to women at elevated risk from fertility treatment or pregnancy. Providers ethically may treat women at elevated risk provided that they are carefully assessed; that specialists in their medical condition are consulted as appropriate; and that patients...
journal_title：Fertility and sterility
pub_type： 杂志文章,实务指引
doi：10.1016/j.fertnstert.2016.08.015
更新日期：2016-11-01 00:00:00
abstract：OBJECTIVE:To report a live birth after successful preimplantation genetic diagnosis (PGD) for carriers of complex chromosomal rearrangements (CCRs) with translocation and deletion. DESIGN:Fluorescent in situ hybridization (FISH) was applied to PGD for CCR carriers. SETTING:University-based centers for reproductive me...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2007.08.016
更新日期：2008-11-01 00:00:00
abstract：:To examine pregnancy rate in patients undergoing controlled ovarian hyperstimulation (COH) with use of multidose GnRH antagonists in multiple repeated IVF cycle attempts, we conducted a survey in 785 consecutive IVF cycles in our unit. Although pregnancy rate significantly decreased beyond the second IVF cycle attempt...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2008.07.1725
更新日期：2009-04-01 00:00:00
abstract：:The morphologic characteristics of 85 cryptorchid and 63 contralateral (scrotal) testes showing histologic changes of puberty were studied. Spermatogenesis was not only delayed on the cryptorchid side but also hypocellular in all cases. The scrotal testes appeared hypocellular in 82.5% of the cases. Repetitive biopsie...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)46346-6
更新日期：1982-06-01 00:00:00
abstract：OBJECTIVE:To evaluate whether microfluidic sperm sorters (MFSSs) allow effective recovery of sorted motile sperm without DNA damage compared with the centrifugation and swim-up procedure. DESIGN:Experimental laboratory study. All participants completed questionnaires regarding previous and/or current diseases, surgery...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2015.10.023
更新日期：2016-02-01 00:00:00
abstract：OBJECTIVE:To correlate the diagnosis of endometriosis in lesions excised at laparoscopy with pathologic diagnosis. DESIGN:Prospective study. SETTING:U.S. government research hospital. PATIENT(S):Women with chronic pelvic pain thought to be due to endometriosis. INTERVENTION(S):Excision of lesions suspicious for end...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(02)03337-x
更新日期：2002-10-01 00:00:00
abstract：:Well-designed and -conducted clinical trials are needed to further advance the field for reproductive medicine. However, current reporting of outcomes of trials is ambiguous and disparate. In this review it is offered that the preferred outcome for clinical trials in reproductive medicine should be live birth. Multipl...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：10.1016/j.fertnstert.2014.03.026
更新日期：2014-05-01 00:00:00
abstract：OBJECTIVE:To compare the expression of the prostaglandin (PG) E(2) transporter multidrug resistance-associated protein 4 (MRP4) in eutopic and ectopic endometrial tissue from endometriosis patients with that of control subjects and to examine whether MRP4 is regulated by the antiinflammatory lipid lipoxin A(4) (LXA(4))...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2013.01.146
更新日期：2013-06-01 00:00:00
abstract：:A comparative pilot study was undertaken in three centers to determine the time of return of ovulation after the injection of the steroidal contraceptive norethisterone oenanthate (NET-OEN). The effect of the compound on the endometrium and some properties of cervical mucus were also assessed. Increases in plasma prog...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(16)45137-x
更新日期：1980-11-01 00:00:00
abstract：:A prospective, randomized, double-blind study with crossover, using tamoxifen (TAM) (Nolvadex, ici Pharma, Missisauga, Canada) 20 mg/day and placebo (p) for 6 months each, was conducted in 16 infertile men with idiopathic oligozoospermia. Baseline sperm count was 17.092 +/- 3.09 (10(6)/ml). Hormonal profile was perfor...
journal_title：Fertility and sterility
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/s0015-0282(16)59299-1
更新日期：1987-07-01 00:00:00
abstract：OBJECTIVE:To compare the rate of ectopic pregnancy (EP) with fresh versus frozen blastocyst transfer in our program during the same time period. DESIGN:Retrospective analysis. SETTING:University IVF program. PATIENT(S):Women who achieved a clinical pregnancy after IVF between 1998 and 2005. INTERVENTION(S):In our p...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/j.fertnstert.2006.12.005
更新日期：2007-09-01 00:00:00
abstract：OBJECTIVE:To determine the sensitivity of 11beta-hydroxyandrostenedione (11-OHA4) and delta5-androstenediol (ADIOL) as markers of excessive adrenal androgen production. DESIGN:Prospective study. SETTING:Academic medical centers. PATIENT(S):Thirteen women with untreated 21-hydroxylase-deficient nonclassic adrenal hyp...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：10.1016/s0015-0282(99)00402-1
更新日期：1999-12-01 00:00:00
abstract：OBJECTIVE:To review the effectiveness of rectal probe electroejaculation in terms of pregnancy in treatment of anejaculatory males due to spinal cord injury. The characteristics of electroejaculates also are examined. DATA IDENTIFICATION:All English language studies reporting pregnancies as a result of electroejaculat...
journal_title：Fertility and sterility
pub_type： 杂志文章,评审
doi：
更新日期：1995-07-01 00:00:00
abstract：:One hundred four consecutive men who underwent a semen analysis as part of their infertility evaluation were assayed for sperm-associated immunoglobulin (Ig) and the third component of complement (C3). Sixteen (15%) of the 104 men had increased IgG, and 10 (12%) of 86 men tested had increased IgA on their sperm; howev...
journal_title：Fertility and sterility
pub_type： 杂志文章
doi：
更新日期：1983-04-01 00:00:00
