Abstract:
:Aberrant cyclin expression has been implicated in oncogenesis in a number of human cancers. Since altered function of regulators of cyclin-dependent kinase (CDK) activity other than cyclins, in particular CDK inhibitors, might play a similar role in oncogenesis, we examined the expression and regulation of the CDK inhibitors p16INK4, p15INK4B and p21WAF1/CIP1 in human breast cancer cell lines. Both the INK4 and INK4B genes were homozygously deleted in 3 cell lines, while INK4 alone was deleted in 2 cell lines. A further 2 cell lines displayed loss of an allele at this locus, and in 1 of these the remaining allele contained a mis-sense mutation within the coding region of the p16INK4 protein. The majority of cell lines examined, including 2 normal mammary epithelial cell strains, expressed low levels of INK4 mRNA and low or undetectable levels of INK4B mRNA. However, INK4 mRNA was expressed at high levels in 5 cell lines, and this was associated with deletion or inactivation of the retinoblastoma susceptibility gene product pRB but not with mutation of TP53. No deletions of the WAF1/CIP1 gene were observed, but WAF1/CIP1 mRNA levels were reduced in cell lines with TP53 mutation. Transfection of a p16INK4 expression vector into MDA-MB-231 cells lacking the INK4 gene failed to produce any p16INK4-expressing cell lines, suggesting that such cells were selected against in continuous culture. Despite the frequent deletion of INK4 in breast cancer cell lines, no evidence was obtained for INK4 deletions in DNA from 45 primary breast carcinomas. Thus, homozygous deletion of the INK4 gene appears to be a rare event in primary breast cancer.
journal_name
Int J Cancerjournal_title
International journal of cancerauthors
Musgrove EA,Lilischkis R,Cornish AL,Lee CS,Setlur V,Seshadri R,Sutherland RLdoi
10.1002/ijc.2910630420subject
Has Abstractpub_date
1995-11-15 00:00:00pages
584-91issue
4eissn
0020-7136issn
1097-0215journal_volume
63pub_type
杂志文章abstract::Our aim was to analyze the expression of the serine protease HtrA1 in human bladder tissue and urine in order to point out its possible association with the presence of urothelial bladder cancer. Bladder tissue and urine specimens from cancer patients with different tumor grades and stages (n = 68) and from individual...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.28280
更新日期:2013-12-01 00:00:00
abstract::Two genes encoding the latent membrane protein 1 (LMP1) of the Epstein-Barr virus (EBV) were isolated from a single case of Hodgkin's disease (HD) and were tested for their biological activities. The LMP1 gene from the Reed-Sternberg cells contained point mutations relative to the prototype LMP1 gene, leading to amino...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980413)76:2<194::aid-ijc
更新日期:1998-04-13 00:00:00
abstract::The roles of fibroblast growth factor-2 (FGF-2) in the hepatocellular carcinoma (HCC) development are still controversial. In this study, we investigated the expression of FGF-2 in chronic hepatitis (CH) type C patients with or without HCC and the immunoregulation of FGF-2 in NK sensitivity of HCC cells. The FGF-2 exp...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.26003
更新日期:2012-01-15 00:00:00
abstract::By rosetting with SRBC coupled to rabbit-anti-human IgM, the surface IgM-negative cells of human fetal bone marrow were enriched, and subsequently infected and transformed by Epstein-Barr virus (EBV). Single clones of the transformed cells were obtained. Ninety percent of the resulting cell clones were surface-immunog...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910390212
更新日期:1987-02-15 00:00:00
abstract::Epigenetic alterations play an important role in carcinogenesis. Recent studies suggested that global histone modifications are predictors of cancer recurrence in various tumor entities. Our study was performed to evaluate histone H3 lysine 4 mono-methyl (H3K4me1), -di-methyl (H3K4me2) and -trimethyl (H3K4me3) pattern...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25250
更新日期:2010-11-15 00:00:00
abstract::The molecular mechanisms underlying the genesis and progression of oligodendroglial tumors are poorly understood, since only restricted information on loss of heterozygosity from isolated cases is available. The commonest alterations appear to involve deletion of 1p and 19q, while loss of heterozygosity for 9p, chromo...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910640311
更新日期:1995-06-22 00:00:00
abstract::Novel classes of drug that interfere with the signalling of the small G-protein Ras, the so-called Ras antagonists, are showing much promise as novel anti-cancer agents. In this study, we demonstrate that the novel Ras antagonist farnesylthiosalicylic acid (FTS) inhibits the growth of Colo 853 melanoma cells through a...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10213
更新日期:2002-04-01 00:00:00
abstract::We show that siRNA-mediated suppression of protein tyrosine phosphatase alpha (PTP alpha) reduces Src activity 2 to 4-fold in breast, colon and other human cancer cell lines. Src and PTP alpha RNAi induced apoptosis in estrogen receptor (ER)-negative breast cancer and colon cancer cells, but not in immortalized noncan...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.23321
更新日期:2008-05-01 00:00:00
abstract::Alcohol consumption is associated with a modest increased risk of colon cancer, but its relationship with colon cancer survival has not been elucidated. Using data from a phase III randomized adjuvant trial, we assessed the association of alcohol consumption with colon cancer outcomes. Patients completed a risk factor...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.30135
更新日期:2016-09-01 00:00:00
abstract::In our previous study on hepatocellular carcinoma (HCC) susceptibility genes in chronic hepatitis patients, we identified the MHC class I polypeptide-related sequence A (MICA). Natural killer cells eliminate various cancer cells, including HCC, by suppressing MICA shedding. Therefore, we investigated MICA sheddases an...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31615
更新日期:2018-11-15 00:00:00
abstract::HNPCC is an autosomal dominantly inherited cancer-susceptibility syndrome that confers an increased risk for colorectal cancer and endometrial cancer at a young age. It also entails an increased risk of a variety of other tumors, such as ovarian, gastric, uroepithelial and biliary tract cancers. The underlying pathoge...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.10240
更新日期:2002-04-10 00:00:00
abstract::In light of recent, strong inverse findings between lifetime red wine consumption and prostate cancer among younger men, we revisited our previous cohort analysis to more thoroughly investigate red wine consumption and prostate cancer in the Health Professionals Follow-up Study (HPFS). In 1986, HPFS participants repor...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22498
更新日期:2007-04-01 00:00:00
abstract::Transcriptional profiling was performed on 452 RNA preparations isolated from various types of pancreatic tissue from tumour patients and healthy donors, with a particular focus on peritumoral samples. Pancreatic ductal adenocarcinomas (PDAC) and cystic tumours were most different in these non-tumorous tissues surroun...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31087
更新日期:2018-03-01 00:00:00
abstract::Pancreatic tumors overexpress FGF-2 and t-PA, but the implication of the growth factor in t-PA synthesis and t-PA-dependent tumor invasion remains unknown. FGF-2 is present in different isoforms: The 18 kDa FGF-2 is secreted, while the 22.5 kDa one is nuclearized and exerts intracrine regulations bypassing cell-surfac...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(20000215)85:4<555::aid-ijc
更新日期:2000-02-15 00:00:00
abstract::The relationship between renal-cell cancer (RCC) and occupation was investigated in an international multicenter population-based case-control study. Study centers in Australia, Denmark, Germany, Sweden and the United States interviewed 1732 incident RCC cases and 2309 controls. Significant associations were found wit...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.2910610503
更新日期:1995-05-29 00:00:00
abstract::Active oxygen (AO) is ubiquitous in nature and its many forms can act as natural carcinogens. Their effect on the transformation of a mouse myeloid progenitor cell line was studied using anchorage-independent colony formation in methylcellulose as the primary assay. Both cytotoxic and non-toxic concentrations of t-but...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910490519
更新日期:1991-11-11 00:00:00
abstract::As a key enzyme in folate metabolism, the thymidylate synthase (TS) is important for the synthesis of nucleotides. Its polymorphisms may affect the TS gene expression and the susceptibility for Fluoropyrimidine (FU)-based chemotherapies. In this study, we assessed the relationship between the TS genotypes and clinical...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25096
更新日期:2010-08-15 00:00:00
abstract::During the course of a mechanism-based screening program aimed at identifying new antimitotic agents, a novel microtubule depolymerizing piperazine derivative, 1-(5-chloro-2-methoxybenzoyl)-4-(3-chlorophenyl) piperazine, was identified. The compound, designated CB694, caused inhibition of proliferation of a wide range...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.21424
更新日期:2006-02-15 00:00:00
abstract::The coincubation of liposomes with mouse leukemia cells or fibroblasts can cause a wide of effects on their viability. These effects are not particular specific in terms of destroying the leukemic cells. However, it was observed that the combination of phosphorylcholine-cholesterol (PC-CHOL)1-liposomes, which are not ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910270616
更新日期:1981-06-15 00:00:00
abstract::Turkey poults were inoculated with lymphoproliferative disease virus (LPDV) of turkey, and the organotropism of the inoculated virus was determined from the kinetics of virus expression and tumor formation. Molecular hybridization experiments, conducted to determine the level of viral RNA expression in the various org...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910290518
更新日期:1982-05-15 00:00:00
abstract::Although early detection and systemic therapies have improved the diagnosis and clinical cure rate of breast cancer, breast cancer remains the most frequently occurring malignant cancer in women due to a lack of sufficiently effective treatments. Thus, to develop potential targeted therapies and thus benefit more pati...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.32922
更新日期:2020-07-15 00:00:00
abstract::BRMS1 is regarded as a metastasis suppressor gene for its ability to reduce metastatic potential of human and murine breast cancer cells as well as human melanoma cells. However, BRMS1 association to human tumor progression is not clearly understood. In the present study we analyzed BRMS1 mRNA expression in tumor prog...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.22379
更新日期:2007-03-15 00:00:00
abstract::Telomerase appears to be an important factor for the control of cellular proliferation capacity and for tumorigenesis. Enzyme activity is highly increased in almost all human tumors and distinguishes them from benign lesions. Besides its diagnostic value, telomerase activity appears to be associated with tumor progres...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19980220)79:1<8::aid-ijc2>
更新日期:1998-02-20 00:00:00
abstract::Glioblastoma multiforme (GBM) is the most lethal brain malignancy which involves multi-gene abnormality. Unfortunately, effective therapy against GBM remains lacking. Previously, we found that NRP-1 and its downstream NRP-1/GIPC1 pathway played an important role in GBM. In our study, we further investigated the upstre...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.31329
更新日期:2018-08-01 00:00:00
abstract::With the improvement of treatments, a growing number of survivors with childhood or adolescent central nervous system (CNS) tumor are parenting their own children. We aimed to explore the risk of somatic diseases among children of these survivors compared to population controls. Children of survivors with CNS tumor be...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.33394
更新日期:2020-11-13 00:00:00
abstract::The objectives of this prospective multicentre international cohort study are to describe the characteristics of a cohort of HIV-1 positive women and determine the best management system by comparing cervical pathology according to results of cytology, colposcopy and human papillomavirus (HPV) testing at baseline and ...
journal_title:International journal of cancer
pub_type: 杂志文章,多中心研究
doi:10.1002/ijc.22947
更新日期:2007-12-01 00:00:00
abstract::Interleukin-11(rhIL-11) is a cytokine that has been shown to enhance the recovery of bone marrow and intestinal crypt cells after cytotoxic insult with radiation or anticancer drugs. The current study examined the effects of rhIL-11 on the response of CEM human lymphoblastic leukemia cells and on the EMT-6 murine mamm...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(SICI)1097-0215(19960917)67:6<864::AID-IJC
更新日期:1996-09-17 00:00:00
abstract::The stroma of ductal infiltrating carcinoma of the human breast shows characteristic and localized areas of collagen rarefaction and fragmentation. This finding has been correlated with a peculiar type of fibrillar damage, observed in a small percentage of collagen fibrils isolated in the native state from the tumour ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.2910390510
更新日期:1987-05-15 00:00:00
abstract::Ciclopirox olamine (CPX) is a synthetic antifungal agent clinically used to treat mycoses of the skin and nails. Here, we show that CPX inhibited tumor growth in human breast cancer MDA-MB-231 xenografts. To unveil the underlying mechanism, we further studied the antitumor activity of CPX in cell culture. The results ...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/ijc.25255
更新日期:2010-11-15 00:00:00
abstract::The effects of androgen manipulation on epidermal growth factor (EGF) receptor, p185erbB-2 and transforming growth factor-alpha (TGF-alpha) levels were examined in prostatic adenocarcinoma. Male nude mice were inoculated with the CWR22 androgen-dependent human prostatic tumor or an androgen-independent (CWR22R) deriva...
journal_title:International journal of cancer
pub_type: 杂志文章
doi:10.1002/(sici)1097-0215(19990730)82:3<424::aid-ijc
更新日期:1999-07-30 00:00:00