Abstract:
:Neural expression of constitutive hsc70 mRNA and hyperthermia-inducible hsp70 mRNA is examined using radioactive and non-radioactive in situ hybridization procedures. A strong induction of hsp70 mRNA was noted in cell populations in cerebellar layers and in the brainstem which demonstrated expression of mRNA encoding proteolipid protein, an oligodendrocyte marker. The non-radioactive in situ hybridization procedure using digoxigenin (DIG)-UTP-labeled riboprobes permitted improved signal localization, and stress-inducible hsp70 mRNA was detected at the cytoplasmic cap areas of individual oligodendrocytes. Cell types which express constitutive members of the hsc/hsp70 multigene family were also identified. Neurons in the brainstem and in the deep white matter and molecular layer of the cerebellum showed expression of hsc70 mRNA while signal was not detected in adjacent glial cells. A neuron-specific enolase riboprobe aided in the identification of neuronal cell types. The non-radioactive DIG riboprobe revealed that hsc70 mRNA was highly localized to the cytoplasm of individual neurons. High constitutive levels of hsc70 in certain neurons may dampen hsp70 induction after hyperthermia in these cell populations.
journal_name
J Neurosci Resjournal_title
Journal of neuroscience researchauthors
Foster JA,Rush SJ,Brown IRdoi
10.1002/jnr.490410506subject
Has Abstractpub_date
1995-08-01 00:00:00pages
603-12issue
5eissn
0360-4012issn
1097-4547journal_volume
41pub_type
杂志文章abstract::An experimental model based on kainic acid (KA) injections replicates many phenomenological features of human temporal lobe epilepsy, the most common type of epilepsy in adults. Taurine, 2-aminoethanesulfonic acid, present in high concentrations in many invertebrate and vertebrate systems, is believed to serve several...
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pub_type: 杂志文章
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更新日期:2002-10-01 00:00:00
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pub_type: 杂志文章
doi:10.1002/jnr.21503
更新日期:2008-02-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.23577
更新日期:2015-08-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.10877
更新日期:2004-02-15 00:00:00
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doi:10.1002/jnr.24076
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doi:10.1002/(SICI)1097-4547(19980301)51:5<563::AID-JNR
更新日期:1998-03-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.1097
更新日期:2001-06-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.21597
更新日期:2008-05-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
doi:10.1002/jnr.490430103
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更新日期:1998-08-01 00:00:00
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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journal_title:Journal of neuroscience research
pub_type: 杂志文章
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更新日期:1985-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2006-05-01 00:00:00
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