Abstract:
:The state of association of bovine Band 3, which is a major intrinsic protein of erythrocyte membranes, was examined in nonaethyleneglycol dodecyl ether (C12E9) solution by ultracentrifugation, gel filtration, gel electrophoresis and cross-linking studies. The molecular weight of bovine Band 3 was 107,000 +/- 5% as determined by gel electrophoresis in the presence of sodium dodecyl sulfate. When Band 3 was purified in C12E9 solution with the aid of 2,3-dimethylmaleic anhydride (DMMA), which is a known dissociating reagent for peripheral proteins from erythrocyte membranes and for Band 3-Band 4.2 complex, the protein was present as a monomer and was not oxidatively cross-linked. On the other hand, DMMA-untreated Band 3 was present as oligomeric forms composed mainly of the dimer and tetramer, and the oligomer in C12E9 was a susceptible to oxidative cross-linking as Band 3 in ghosts. The oligomeric form apparently retained a more ordered conformational state than the monomeric form. These results indicate that the bovine Band 3 oligomer is a stable form in C12E9, but the present result showing the coexistence of dimer and tetramer in C12E9 contrasts with the reported observation that human Band 3 is present as a stable dimer in a nonionic detergent, Triton X-100. It was shown that polyacrylamide gradient gel electrophoresis in the presence of C12E9 gave better resolution of the associated species of Band 3 than ultracentrifugation or gel filtration, and this method made it possible to determine the Stokes radii of Band 3-C12E9 complexes. This result suggests the usefulness of electrophoretic methods in the presence of nonionic detergent for studies of the state of association of other membrane proteins.
journal_name
J Biochemjournal_title
Journal of biochemistryauthors
Nakashima H,Makino Sdoi
10.1093/oxfordjournals.jbchem.a133081subject
Has Abstractpub_date
1980-10-01 00:00:00pages
933-7issue
4eissn
0021-924Xissn
1756-2651journal_volume
88pub_type
杂志文章abstract::Spleen cells from a CBF1 (BALB/c X C57BL/6) mouse immunized with rat tyrosine 3-monooxygenase were fused with NS-1 mouse myeloma cells. From 188 hybrid cells, 2 stable clones secreting anti-tyrosine 3-monooxygenase antibody were obtained. Antibody from one clone was coupled to CNBr-activated Sepharose 4B and the monoc...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135050
更新日期:1985-01-01 00:00:00
abstract::Hepatic delta-aminolevulinate (ALA) synthetase was induced in mice by the administration of allylisopropylacetamide (AIA) and 3,5-dicarbethoxy-1,4-dihydrocollidine (DDC). In both cases, a significant amount of ALA synthetase accumulated in the liver cytosol fraction as well as in the mitochondria. The apparent molecul...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131365
更新日期:1976-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131272
更新日期:1976-08-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1998-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:2007-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2017-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123399
更新日期:1991-03-01 00:00:00
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更新日期:2007-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123023
更新日期:1990-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1983-12-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a122292
更新日期:1988-03-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a133678
更新日期:1982-01-01 00:00:00
abstract::The sugar chain of bovine pancreatic ribonuclease B was released from the polypeptide moiety by hydrazinolysis and by endo-beta-N-acetylglucosaminidase H digestion, and reduced with NaB[3H]4 after N-acetylation. The radioactive oligosaccharide mixtures thus obtained were fractionated by Bio-Gel P-4 column chromatograp...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:
更新日期:1980-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a131660
更新日期:1977-07-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a003247
更新日期:2002-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134806
更新日期:1984-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:1989-11-01 00:00:00
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更新日期:1982-12-01 00:00:00
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更新日期:2000-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1983-08-01 00:00:00
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journal_title:Journal of biochemistry
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更新日期:2015-06-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a022494
更新日期:1999-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a134877
更新日期:1984-09-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
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更新日期:1993-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a123417
更新日期:1991-04-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/jb/mvj013
更新日期:2006-01-01 00:00:00
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journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a135556
更新日期:1986-03-01 00:00:00
abstract::A 2-hydroxychromene-2-carboxylate isomerase was purified from a cell-free extract of naphthalenesulfonate-assimilating Pseudomonas sp. TA-2 to an electrophoretically homogeneous state by successive column chromatography on DEAE-cellulose, DEAE-Toyopearl 650M, Sephadex G-75, Hydroxyapatite, and Mono Q. The enzyme had a...
journal_title:Journal of biochemistry
pub_type: 杂志文章
doi:10.1093/oxfordjournals.jbchem.a022152
更新日期:1998-09-01 00:00:00