Abstract:
:Selective and differential media were designed for each species of Pityrosporum; P. pachydermatis, P. ovale, and P. orbiculare in order to make feasible a quantitative cultivation. Medium for P. pachydermatis (medium A) was composed of 1% trypticase peptone (BBL), 0.5% yeast extract (BBL), 0.3% glucose, 0.2% NaCl, 1.2% KH2 PO4 (anhydrous), 1.5% agar, 0.01% ampicillin, and 0.025% cycloheximide with a pH of 5.5. Medium for P. ovale (medium B) was medium A supplemented with 0.05% sodium acetate (anhydrous), 0.2% Tween 80, and 0.025% (selective medium) or 0.075% (differential medium) sodium laurate. Medium for P. orbiculare was medium B (devoid of laurate) supplemented with 2% olive oil, 0.25% glycerol, 0.25% gall powder, 0.05% sodium palmitate, 0.05% sodium stearate, 0.05% sodium oleate and 8% (selective medium) or 10% (differential medium) sodium lactate and an increase in Tween to 1%. For isolation of Pityrosporum, specimens were suspended in 0.1% Tween 80 solution and inoculated onto agar plates of three selective media. The plates were incubated aerobically at 37 C for 8-10 days under conditions of prevention of water loss from the media. The plating efficiency of each selective medium, expressed as a ratio of cultural counts to microscopic counts was generally over 70%. Species of Pityrosporum could also be identified when we inoculated the cell suspension onto differential agar plates and incubated the preparations at 37 C for 7 days.
journal_name
Microbiol Immunoljournal_title
Microbiology and immunologyauthors
Ushijima T,Takahashi M,Ozaki Ydoi
10.1111/j.1348-0421.1981.tb00119.xsubject
Has Abstractpub_date
1981-01-01 00:00:00pages
1109-18issue
11eissn
0385-5600issn
1348-0421journal_volume
25pub_type
杂志文章abstract::The use of continuous free-flow electrophoresis for the purification of extracted lipopolysaccharides ( LPSs ) was investigated. Commercial (nucleic acid contaminated) LPS preparations, isolated by the hot phenol-water method of Westphal from Salmonella typhimurium and Escherichia coli 0111: B4, were analyzed. Continu...
journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1984.tb00668.x
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journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1998-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1985-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章,评审
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pub_type: 杂志文章,评审
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更新日期:1984-01-01 00:00:00
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更新日期:1994-01-01 00:00:00
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pub_type: 杂志文章
doi:10.1111/j.1348-0421.1982.tb00151.x
更新日期:1982-01-01 00:00:00
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journal_title:Microbiology and immunology
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:2000-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
doi:10.1111/j.1348-0421.1987.tb03134.x
更新日期:1987-01-01 00:00:00
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journal_title:Microbiology and immunology
pub_type: 杂志文章
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更新日期:1985-01-01 00:00:00
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pub_type: 杂志文章
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