Abstract:
:When chromosomal proteins in chromatin or in mononucleosomes were extensively cross-linked with an imido ester, the H1-containing nonameric histone complex was revealed. In this complex, histone H1 is connected with the octamer of core histones. The cross-linking of H1 of the octamer is realized preferentially through H2a and H3 histones. Some HMG (high mortality group) proteins located presumably in the linker regions of a nucleosome fiber also take part in the formation of dimers, possibly with the histones of a nucleosomal core. The results suggest mutant interactions between some linker-associated proteins and intranucleosomal histones. Experiments involving extensive cross-linking of proteins in the purified mononucleosome subfractions demonstrated differences in the organization of core histones between 'complete' nucleosomes and nucleosomes lacking H1.
journal_name
Mol Biol Repjournal_title
Molecular biology reportsauthors
Bakayev VV,Bakayeva TG,Domansky NNdoi
10.1007/BF00805754subject
Has Abstractpub_date
1981-08-14 00:00:00pages
209-16issue
4eissn
0301-4851issn
1573-4978journal_volume
7pub_type
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journal_title:Molecular biology reports
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