Abstract:
:A quantitative bioassay for serum erythropoietin in anemic patients was established with erythroid colony-forming technique using methyl cellulose. Fetal mouse liver contains many erythropoietin-dependent erythroid colony-forming cells (CFU-E) with the concentration as well as sensitivity to erythropoietin being the highest at 13-14 days of gestation. Linearity in the erythropoietin response curve for the number of colonies from CFU-E in fetal livers of 13-14 days gestation was obtained for erythropoietin concentrations in the culture medium of 3.9 to 125 mU/ml. When newborn liver cells were used instead, the linearity occurred at 31.2 to 250 mU/ml. Measurement of erythropoietin by the erythroid colony-forming technique for sera from patients with various anemias was compared with that of the assay method using radioiron incorporation into heme in the suspension culture of fetal mouse liver cells. There was a good correlation between the values of both assay methods (r = 0.856, p less than 0.001). Also, levels of erythropoietin measured by this assay method showed the expected inverse relationship to the hemoglobin concentration in the sera from the patients.
journal_name
Am J Hematoljournal_title
American journal of hematologyauthors
Okamoto T,Kanamaru A,Hara H,Nagai Kdoi
10.1002/ajh.2830120210subject
Has Abstractpub_date
1982-04-01 00:00:00pages
179-86issue
2eissn
0361-8609issn
1096-8652journal_volume
12pub_type
杂志文章abstract::Lentiviral modification combined with ex vivo erythroid differentiation was used to stably inhibit RhAG expression, a critical component of the Rh(rhesus) membrane complex defective in the Rh(null) syndrome. The cultured red cells generated recapitulate the major alterations of native Rh(null) cells regarding antigen ...
journal_title:American journal of hematology
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abstract::Some patients suffering from malignancies may benefit of myeloablative chemotherapy followed by hematological reconstitution with autologous peripheral blood reinfusion. A quick evaluation of the number of hematopoietic progenitors present in leukapheresis blood samples is necessary to ensure the collection of a suffi...
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journal_title:American journal of hematology
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journal_title:American journal of hematology
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abstract::Chuvash polycythemia results from a homozygous 598C>T mutation in exon 3 of the von Hippel-Lindau (VHL) gene. This disrupts the normoxia pathway for degrading hypoxia inducible factor (HIF)-1alpha and HIF-2alpha causing altered expression of HIF-1 and HIF-2 inducible genes. As hypoxia induces expression of pro-inflamm...
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journal_title:American journal of hematology
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更新日期:1983-09-01 00:00:00
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journal_title:American journal of hematology
pub_type: 杂志文章
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更新日期:1996-03-01 00:00:00
abstract::Responsiveness of bone marrow erythropoietic stem cells (CFU-E and BFU-E) to recombinant human erythropoietin (rh-Ep) was examined in vitro in 23 patients with aplastic anemia and 14 with myelodysplastic syndrome (MDS) to investigate the clinical use of rh-Ep for these diseases. Bone marrow mononuclear cells were cult...
journal_title:American journal of hematology
pub_type: 杂志文章
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abstract::Karyotype is frequently used to predict response and outcome in leukemia. This post hoc exploratory analysis evaluated the relationship between baseline cytogenetics and outcome in patients with relapsed/refractory acute lymphoblastic leukemia (R/R ALL) treated with inotuzumab ozogamicin (InO), a humanized CD22 antibo...
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更新日期:2012-12-01 00:00:00
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journal_title:American journal of hematology
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更新日期:2005-03-01 00:00:00
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journal_title:American journal of hematology
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更新日期:2018-12-01 00:00:00
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pub_type: 临床试验,杂志文章,多中心研究
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更新日期:2014-10-01 00:00:00
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