Effects of humic acid on the viability and coagulant properties of human umbilical vein endothelial cells.


:We have previously shown that humic acid (well-water humic acid, HA, and synthetic humic acid, SHA) enhances cell surface expression of tissue factor (TF). Here we report that incubation of human umbilical vein endothelial cells (HUVEC) for 2 hr with HA or SHA cause a rapid rise in TF mRNA levels, as shown by Northern blot analysis. To understand the cytotoxic and fibrinolytic effects of HA and SHA on cultured HUVEC, the cells treated with varying concentrations of HA and SHA for various periods of time. Both HA and SHA (10-200 micrograms/ml) inhibited the viability of subconfluent HUVEC, cultured in the presence or absence of 20% FBS (Fetal Bovine serum) in the culture medium, in a dose-dependent manner. Both HA and SHA induced surface changes in the HUVEC as revealed by scanning electron micrography (SEM). However, protocatechuic acid, the monomer of SHA, did not significantly inhibit cell growth, and showed a cytotoxic effect only at 200 micrograms/ml. Furthermore both HA and SHA stimulated HUVEC to produce plasminogen activator inhibitor (PAI-1) and tissue plasminogen activator (t-PA) in a dose and time dependent fashion; the amount of PAI-1 produced was found to exceed that of t-PA. The monomer of SHA did not have this stimulatory effect. These results distinctly suggest that in addition to the inhibition of viability HA is involved in TF induction and PAI-1 synthesis in HUVEC and these may be some of the plausible mechanisms underlying the thrombotic disorders in Blackfoot disease.


Am J Hematol


Yang HL,Chiu HC,Lu FJ




Has Abstract


1996-03-01 00:00:00














  • Monocyte transferrin-iron uptake in hereditary hemochromatosis.

    abstract::Transferrin-iron uptake by peripheral blood monocytes was studied in vitro to test the hypothesis that the relative paucity of mononuclear phagocyte iron loading in hereditary hemochromatosis results from a defect in uptake of iron from transferrin. Monocytes from nine control subjects and 17 patients with hemochromat...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Sizemore DJ,Bassett ML

    更新日期:1984-05-01 00:00:00

  • Detection of monosomy 7 in interphase cells of patients with myeloid disorders.

    abstract::Six patients, five with acute myeloid leukemia (AML) and one with a myelodysplastic syndrome (MDS), were found to have monosomy 7 by conventional cytogenetics at diagnosis. Repetitive DNA sequences from the heterochromatic region of human chromosomes 1 and 7 were used as probes for in situ hybridization experiments on...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Kolluri RV,Manuelidis L,Cremer T,Sait S,Gezer S,Raza A

    更新日期:1990-02-01 00:00:00

  • Elevated soluble IL-2Rα, IL-8, and MIP-1β levels are associated with inferior outcome and are independent of MIPI score in patients with mantle cell lymphoma.

    abstract::Mantle cell lymphoma (MCL) is a unique type of lymphoma with a prognosis intermediate between indolent and aggressive types. The purpose of this study was to study blood cytokine levels in newly diagnosed and relapsed MCL patients with respect to patterns of abnormalities and relationship to the MCL International Prog...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Sonbol MB,Maurer MJ,Stenson MJ,Allmer C,LaPlant BR,Weiner GJ,Macon WR,Cerhan JR,Witzig TE,Gupta M

    更新日期:2014-12-01 00:00:00

  • UGT2B7 promoter variant -840G>A contributes to the variability in hepatic clearance of morphine in patients with sickle cell disease.

    abstract::The purpose of the study was to determine if UDP-glucuronosyltransferase (UGT) 2B7 allelic variants encoding for UGT2B7, primary enzyme responsible for morphine glucuronidation contribute to the variability in the hepatic clearance of morphine in sickle cell disease (SCD). Twenty-four hour PK study of morphine and UGT...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Darbari DS,van Schaik RH,Capparelli EV,Rana S,McCarter R,van den Anker J

    更新日期:2008-03-01 00:00:00

  • Incidence, management, and outcome of high-grade transformation of nodular lymphocyte predominant Hodgkin lymphoma: long-term outcomes from a 30-year experience.

    abstract::Nodular lymphocyte predominant Hodgkin lymphoma (NLPHL) is a rare form of Hodgkin lymphoma that typically presents as early stage, indolent disease in young adult males. The relationship between NLPHL and DLBCL is incompletely understood, and there remains a paucity of data with regard the incidence and management of ...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Eyre TA,Gatter K,Collins GP,Hall GW,Watson C,Hatton CS

    更新日期:2015-06-01 00:00:00

  • Peripheral blood remission of hairy cell leukemia after transfusion hepatitis.

    abstract::Hairy cell leukemia is a chronic lymphoproliferative disorder characterized clinically by splenomegaly and cytopenias. Spontaneous remissions are rare and splenectomy is often performed when the blood counts worsen and cause symptoms. Three of our patients with hairy cell leukemia developed recurrent pancytopenia and ...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Keefer MJ,Weber MJ,Bottomley SS,Solanki DL,Hosty TA

    更新日期:1987-07-01 00:00:00

  • First-line treatment of 102 chronic myeloid leukemia patients with imatinib: a long-term single institution analysis.

    abstract::Imatinib mesylate radically changed the natural history of chronic myeloid leukemia (CML). The recent availability of alternative tyrosine kinase inhibitors (TKIs) renders the clinical management of CML more complex. In this article, we summarize our long-term single institution experience. From 2003 to 2012, 102 pati...

    journal_title:American journal of hematology

    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Viganò I,Di Giacomo N,Bozzani S,Antolini L,Piazza R,Gambacorti Passerini C

    更新日期:2014-10-01 00:00:00

  • A prospective newborn screening and treatment program for sickle cell anemia in Luanda, Angola.

    abstract::Over 300,000 infants are born annually with sickle cell anemia (SCA) in sub-Saharan Africa, and >50% die young from infection or anemia, usually without diagnosis of SCA. Early identification by newborn screening (NBS), followed by simple interventions dramatically reduced the mortality of SCA in the United States, bu...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: McGann PT,Ferris MG,Ramamurthy U,Santos B,de Oliveira V,Bernardino L,Ware RE

    更新日期:2013-12-01 00:00:00

  • Natural history of acquired immunodeficiency syndrome in hemophilic patients.

    abstract::During the 5-year period from 1981 to 1985, we have observed 8 cases of acquired immunodeficiency syndrome (AIDS) among our 85 patients with hemophilia A. Thus, the prevalence of AIDS with hemophilia A is 9.4% in our patient population. By utilizing stored serum or plasma samples dating back to 1978, antibody against ...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Kim HC,Nahum K,Raska K Jr,Gocke DJ,Kosmin M,Karp GI,Saidi P

    更新日期:1987-02-01 00:00:00

  • High NPM1 mutant allele burden at diagnosis correlates with minimal residual disease at first remission in de novo acute myeloid leukemia.

    abstract::Acute myeloid leukemia (AML) with mutated NPM1 is a newly recognized separate entity in the revised 2016 WHO classification, and is associated with a favorable prognosis. While previous studies have evaluated NPM1 in a binary fashion, we recently demonstrated a significant independent negative prognostic effect of hig...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Patel SS,Pinkus GS,Ritterhouse LL,Segal JP,Dal Cin P,Restrepo T,Harris MH,Stone RM,Hasserjian RP,Weinberg OK

    更新日期:2019-08-01 00:00:00

  • Epstein-Barr virus infection in Richter's transformation.

    abstract::Chronic lymphocytic leukemia (CLL) may convert to a diffuse large cell lymphoma (Richter's syndrome) over time. In occasional cases of Richter's transformation, Epstein-Barr virus (EBV) has been identified in the lymphoma cells. To evaluate the association of EBV infection with Richter's syndrome, the biopsy specimens...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Ansell SM,Li CY,Lloyd RV,Phyliky RL

    更新日期:1999-02-01 00:00:00

  • Test of the month: The chromogenic antifactor Xa assay.

    abstract::As the number of anticoagulant drugs increases and new ones are brought to market, the utility of the routine screening coagulation tests of today--namely the prothrombin time and activated partial thromboplastin time--will be significantly reduced in many clinical situations. Although the new anticoagulants are desig...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Gehrie E,Laposata M

    更新日期:2012-02-01 00:00:00

  • Vasoactive factors in sickle cell disease: in vitro evidence for endothelin-1-mediated vasoconstriction.

    abstract::While systemic plasma endothelin-1 (ET-1) levels are increased during acute crisis in sickle cell disease, the relative levels of potent vasoactive factors that contribute to the regulation of vascular function, such as ET-1, NO, and cell-free hemoglobin, during the course of periodic vaso-occlusive episodes remain un...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Ergul S,Brunson CY,Hutchinson J,Tawfik A,Kutlar A,Webb RC,Ergul A

    更新日期:2004-07-01 00:00:00

  • Many unbalanced translocations show duplication of a translocation participant. Clinical and cytogenetic implications in myeloid hematologic malignancies.

    abstract::If a translocation is followed by loss of one of the two derivative chromosomes, the result is an unbalanced translocation, showing monosomy for the segments making up the lost derivative. We have found that in most unbalanced translocations, a third event takes place: a morphologically normal copy of one of the two t...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Pedersen B,Nørgaard JM,Pedersen BB,Clausen N,Rasmussen IH,Thorling K

    更新日期:2000-07-01 00:00:00

  • Bone marrow failure and myelofibrosis in a case of PVP storage disease.

    abstract::"PVP storage disease" is a disorder occurring in patients who have received high molecular weight polyvinylpyrrolidone (PVP), which cannot be excreted from the body. These large polymers deposit in the histiocytes and cause proliferation and infiltration of histiocytes in the reticuloendothelial system. There was usua...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Dunn P,Kuo T,Shih LY,Wang PN,Sun CF,Chang MJ

    更新日期:1998-01-01 00:00:00

  • Diagnostic workup of acute leukemias of ambiguous lineage.

    abstract::Acute leukemias of ambiguous lineage (ALAL) comprise acute undifferentiated leukemias (AUL) and mixed-phenotype acute leukemias (MPAL). In the revised fourth edition of the World Health Organization (WHO) classification provided further refinements to the diagnostic criteria for ALAL. Molecular characterization of MPA...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Patel SS,Weinberg OK

    更新日期:2020-06-01 00:00:00

  • von Willebrand disease R1374C: type 2A or 2M? A challenge to the revised classification. High frequency in the northwest of Spain (Galicia).

    abstract::Patients initially diagnosed with type 1 von Willebrand disease (VWD) have been reclassified as type 2 after a more exhaustive analysis in several studies. Our study's objectives were (1) to reanalyze patients that were previously diagnosed as type 1 to achieve a more accurate diagnosis and (2) to compare the von Will...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Penas N,Pérez-Rodríguez A,Torea JH,Lourés E,Noya MS,López-Fernández MF,Batlle J

    更新日期:2005-11-01 00:00:00

  • Factor V inhibitor in thrombosis.

    abstract::A 68-year-old previously well woman developed sudden onset of limb gangrene in association with liver dysfunction. An immediately acting inhibitor to factor V with some of the features of lupus anticoagulant was demonstrated. The patient required limb amputation within 2 weeks and activity of the anticoagulant seemed ...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Kapur A,Kelsey PR,Isaacs PE

    更新日期:1993-04-01 00:00:00

  • Red cell ferritin and iron overload in heterozygous beta-thalassemia.

    abstract::Red cell ferritin was evaluated in 101 individuals with heterozygous beta-thalassemia to determine its clinical utility as an index for iron deficiency or overload in these subjects. The mean red cell ferritin for the total population was elevated threefold and showed a significant correlation with transferrin saturat...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Van der Weyden MB,Fong H,Hallam LJ,Harrison C

    更新日期:1989-04-01 00:00:00

  • Developmental and inducible patterns of human theta 1-globin gene expression in embryonic/fetal and adult erythroid cells.

    abstract::Human theta (theta 1)-globin gene represents a member of the alpha-like globin gene family residing on chromosome 16. theta 1-Specific transcripts have been detected so far only in erythroid tissues and in erythroleukemia K562 cells. To investigate systematically its inducible expression and developmental specificity,...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Mamalaki A,Anagnou NP,Moschonas NK

    更新日期:1990-12-01 00:00:00

  • Phase I study of oral clofarabine consolidation in adults aged 60 and older with acute myeloid leukemia.

    abstract::Clofarabine has shown activity and tolerability in older patients with acute myeloid leukemia (AML). We investigated the safety and tolerability of an oral formulation of clofarabine for consolidation therapy of patients aged 60 and older with AML. In this phase I study, twenty-two patients older than 60 years with AM...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Jacoby MA,Martin MG,Uy GL,Westervelt P,Dipersio JF,Cashen A,Stockerl-Goldstein K,Vij R,Luo J,Reineck T,Bernabe N,Abboud CN

    更新日期:2014-05-01 00:00:00

  • CAR T-cells merge into the fast lane of cancer care.

    abstract::Chimeric antigen receptors (CARs) can be introduced into T-cells redirecting them to target specific tumor antigens. CAR-modified T cells targeting CD19 have shown remarkable activity against CD19+ malignancies including B cell acute lymphocytic leukemia (ALL), chronic lymphocytic leukemia (CLL), and non-Hodgkin lymph...

    journal_title:American journal of hematology

    pub_type: 杂志文章,评审


    authors: Frey NV,Porter DL

    更新日期:2016-01-01 00:00:00

  • Laparoscopic splenectomies for idiopathic thrombocytopenic purpura: experience of sixty cases.

    abstract::We performed a laparoscopic splenectomy (LS) in 60 patients (age 9-83, 45 females) with idiopathic thrombocytopenic purpura (ITP) who did not achieve sustained remission on steroid therapy. Using a modified procedure, the mean duration of LS was 78 min (range 25-240 min) and surgery was associated with only 5% major a...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Szold A,Schwartz J,Abu-Abeid S,Bulvik S,Eldor A

    更新日期:2000-01-01 00:00:00

  • Acute myeloid leukemia: 2014 update on risk-stratification and management.

    abstract:OVERVIEW:Evidence suggests that even patients aged 70 or above benefit from specific AML therapy. The fundamental decision in AML then becomes whether to recommend standard or investigational treatment. This decision must rest on the likely outcome of standard treatment. Hence we review factors that predict treatment r...

    journal_title:American journal of hematology

    pub_type: 杂志文章,评审


    authors: Estey EH

    更新日期:2014-11-01 00:00:00

  • Postoperative thrombotic thrombocytopenic purpura following cardiovascular surgeries.

    abstract::Among 47 patients with thrombotic thrombocytopenic purpura (TTP), 8 patients were diagnosed to have postoperative-TTP. Two patients underwent vascular surgery, 5 patients coronary artery bypass grafts, and 1 patient resection of myocardial sarcoma. Prior to surgery, all patients except one had normal hemograms and pla...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Chang JC,Shipstone A,Llenado-Lee MA

    更新日期:1996-09-01 00:00:00

  • Abnormal gamma IFN and alpha TNF secretion in purified CD2+ cells from autoimmune thrombocytopenic purpura (ATP) patients: their implication in the clinical course of the disease.

    abstract::Gamma inferferon (gamma IFN), alpha tumor necrosis factor (alpha TNF), and interleukin 6 (IL-60) are cytokines produced by a wide variety of cells, including T lymphocytes and NK cells. These cytokines affect B-cell proliferation and differentiation into immunoglobulin secreting cells. In addition, gamma IFN and alpha...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Garcia-Suarez J,Prieto A,Reyes E,Manzano L,Arribalzaga K,Alvarez-Mon M

    更新日期:1995-08-01 00:00:00

  • Prevalence of factor V G1691A (factor V-Leiden) and prothrombin G20210A gene mutations in a recurrent miscarriage population.

    abstract::Factor V G1691A (FV-Leiden) and prothrombin G20210A mutations are major inherited risk factors for venous thrombosis. Recently, it was suggested that both mutations, through stimulation of venous and placental thrombosis events, were strongly associated with recurrent idiopathic miscarriages, although other studies di...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Finan RR,Tamim H,Ameen G,Sharida HE,Rashid M,Almawi WY

    更新日期:2002-12-01 00:00:00

  • Febrile transfusion reaction following initial transfusion in a man with immunoblastic lymphadenopathy and granulocyte autoantibodies.

    abstract::A 69-year-old man with immunoblastic lymphadenopathy and autoimmune hemolytic anemia who had no previous exposure to blood products developed a severe febrile nonhemolytic transfusion reaction following the initial infusion of packed red blood cells. The reaction recurred with transfusion of packed red blood cells, bu...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Barnes HM,Poon MC,Huang ST,Conrad ME,Lin J,McGowan EI

    更新日期:1983-02-01 00:00:00

  • Application of a monoclonal antibody specific for the delta chain of hemoglobin A2 in the diagnosis of beta thalassemia.

    abstract::We have developed a murine monoclonal antibody (mAb) specific for the delta chain of hemoglobin (Hb) A2 that does not cross-react with alpha, beta, or gamma chains. The mAb reacted with Hb P-Nilotic (beta delta hybrid), but not with Hb Lepore-Boston (delta beta hybrid), indicating an epitope consisting of positions 11...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Shyamala M,Kiefer CR,Moscoso H,Garver FA

    更新日期:1991-11-01 00:00:00

  • Chronic lymphoproliferative disorder with regulatory T-cell phenotype.

    abstract::We report a case of T-cell chronic lymphoproliferative disorder (CLPD) that shows neither features of T-cell prolymphocytic leukemia nor disease progression for more than 34 months. Flow cytometric analyses of the lymphocytes revealed high expression of CD4 and CD25. Up-regulation of Foxp3, a master regulatory gene fo...

    journal_title:American journal of hematology

    pub_type: 杂志文章


    authors: Kikuchi T,Katayama Y,Kubonishi S,Watanabe T,Watanabe Y,Matsuoka K,Maeda Y,Namba N,Masunari T,Nasu R,Ikeda K,Tanimoto M

    更新日期:2006-09-01 00:00:00