Origin of replication from Xenopus laevis mitochondrial DNA promotes high-frequency transformation of yeast.

abstract：:The ability to dynamically organize functional nanoparticles (NPs) via the use of environmental triggers (temperature, pH, light, or solvent polarity) opens up important perspectives for rapid and convenient construction of a rich variety of complex assemblies and materials with new structures and functionalities. Her...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen Y,Wang Z,He Y,Yoon YJ,Jung J,Zhang G,Lin Z

更新日期：2018-02-13 00:00:00

abstract：:Antibodies can be developed to directly inhibit almost any protein, but their inability to enter the cytosol limits inhibitory antibodies to membrane-associated or extracellular targets. Developing a cytosolic antibody delivery system would offer unique opportunities to directly inhibit and study intracellular protein...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang HH,Tsourkas A

更新日期：2019-10-29 00:00:00

abstract：:We previously have shown that proliferating lymphoid cells contain an extractable cytoplasmic protein that is capable of inducing DNA synthesis in isolated quiescent nuclei (activator of DNA replication, ADR). ADR is present in continuously proliferating (transformed) lymphoblastoid cell lines and in mitogen- or inter...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gutowski JK,West A,Cohen S

更新日期：1985-08-01 00:00:00

abstract：:The majority of biological turnover of lignocellulosic biomass in nature is conducted by fungi, which commonly use Family 1 carbohydrate-binding modules (CBMs) for targeting enzymes to cellulose. Family 1 CBMs are glycosylated, but the effects of glycosylation on CBM function remain unknown. Here, the effects of O-man...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen L,Drake MR,Resch MG,Greene ER,Himmel ME,Chaffey PK,Beckham GT,Tan Z

更新日期：2014-05-27 00:00:00

abstract：:This introduction to the Special Feature presents the context for science during the Deepwater Horizon oil spill response, summarizes how scientific knowledge was integrated across disciplines and statutory responsibilities, identifies areas where scientific information was accurate and where it was not, and considers...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lubchenco J,McNutt MK,Dreyfus G,Murawski SA,Kennedy DM,Anastas PT,Chu S,Hunter T

更新日期：2012-12-11 00:00:00

abstract：:Social behavior is often shaped by the rich storehouse of biographical information that we hold for other people. In our daily life, we rapidly and flexibly retrieve a host of biographical details about individuals in our social network, which often guide our decisions as we navigate complex social interactions. Even ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang Y,Collins JA,Koski J,Nugiel T,Metoki A,Olson IR

更新日期：2017-04-18 00:00:00

abstract：:EcoRI endonuclease-generated DNA fragments carrying replication regions of the F'lac and R6-5 plasmids have been cloned and isolated, using as a selection vehicle a nonreplicating ampicillin-resistance DNA fragment derived from a Staphylococcus aureus plasmid. Heteroduplex analysis of the constructed plasmid chimeras ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Timmis K,Cabello F,Cohen SN

更新日期：1975-06-01 00:00:00

abstract：:We have developed a gene-fusion system based on the Escherichia coli beta-glucuronidase gene (uidA). The uidA gene has been cloned from E. coli K-12 and its entire nucleotide sequence has been determined. beta-Glucuronidase has been purified to homogeneity and characterized. The enzyme has a subunit molecular weight o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jefferson RA,Burgess SM,Hirsh D

更新日期：1986-11-01 00:00:00

abstract：:We describe here a third region of variability in human fibronectin (FN) due to alternative RNA splicing. Two other positions of alternative splicing have been reported previously (ED and IIICS). The third region involves a 273-nucleotide exon encoding exactly one 91-amino acid repeat of type III homology, located bet...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gutman A,Kornblihtt AR

更新日期：1987-10-01 00:00:00

abstract：:Ferroelectricity has long been speculated to have important biological functions, although its very existence in biology has never been firmly established. Here, we present compelling evidence that elastin, the key ECM protein found in connective tissues, is ferroelectric, and we elucidate the molecular mechanism of i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Liu Y,Cai HL,Zelisko M,Wang Y,Sun J,Yan F,Ma F,Wang P,Chen QN,Zheng H,Meng X,Sharma P,Zhang Y,Li J

更新日期：2014-07-08 00:00:00

abstract：:Suppressor T cells have been shown to be much more radiosensitive than other lymphoïd cells, and we have tried to reduce tumor growth by low-dose irradiation. Syngeneic DBA/2 mice received whole-body irradiation (150 rads; 1 rad = 0.01 J/kg) 6 days after P815 tumor inoculation. Tumor growth is significantly reduced in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tilkin AF,Schaaf-Lafontaine N,Van Acker A,Boccadoro M,Urbain J

更新日期：1981-03-01 00:00:00

abstract：:In this paper, we describe the structure of chitinase B from Serratia marcescens, which consists of a catalytic domain with a TIM-barrel fold and a 49-residue C-terminal chitin-binding domain. This chitinase is the first structure of a bacterial exochitinase, and it represents one of only a few examples of a glycosyl ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van Aalten DM,Synstad B,Brurberg MB,Hough E,Riise BW,Eijsink VG,Wierenga RK

更新日期：2000-05-23 00:00:00

abstract：:We report on the design and characterization of a class of biomolecular interfaces based on derivatized poly(l-lysine)-grafted poly(ethylene glycol) copolymers adsorbed on negatively charged surfaces. As a model system, we synthesized biotin-derivatized poly(l-lysine)-grafted poly(ethylene glycol) copolymers, PLL-g-[(...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ruiz-Taylor LA,Martin TL,Zaugg FG,Witte K,Indermuhle P,Nock S,Wagner P

更新日期：2001-01-30 00:00:00

abstract：:The murine-virus-infected erythroleukemia cell system provides an opportunity to examine regulatory mechanisms controlling cytodifferentiation. A cloned cell line (DR10c3) resistant to the erythropoiesis-inducing effect of dimethylsulfoxide (Me2SO) was isolated from the Me2SO-sensitive line DS19. DR10c3 is characteriz...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ohta Y,Tanaka M,Terada M,Miller OJ,Bank A,Marks P,Rifkind RA

更新日期：1976-04-01 00:00:00

abstract：:Early members of the dinosaur-pterosaur clade Ornithodira are very rare in the fossil record, obscuring our understanding of the origins of this important group. Here, we describe an early ornithodiran (Kongonaphon kely gen. et sp. nov.) from the Mid-to-Upper Triassic of Madagascar that represents one of the smallest ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kammerer CF,Nesbitt SJ,Flynn JJ,Ranivoharimanana L,Wyss AR

更新日期：2020-07-28 00:00:00

abstract：:We present a model describing how Mg(2+) binds and stabilizes specific RNA structures. In this model, RNA stabilization arises from two energetically distinct modes of Mg(2+) binding: diffuse- and site-binding. Diffusely bound Mg(2+) are electrostatically attracted to the strong anionic field around the RNA and are ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Misra VK,Draper DE

更新日期：2001-10-23 00:00:00

abstract：:In RBL-2H3 rat tumor mast cells, cross-linking the high-affinity IgE receptor Fc epsilon RI causes tyrosine phosphorylation of multiple proteins. These phosphoproteins include phospholipase C gamma 1, the beta and gamma subunits of the Fc epsilon RI, the Src family protein-tyrosine kinase Lyn, and a 72-kDa protein tha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hutchcroft JE,Geahlen RL,Deanin GG,Oliver JM

更新日期：1992-10-01 00:00:00

abstract：:Anaerobic glycolysis in Saccharomyces cerevisiae has been studied by 13C NMR at 90.5 MHz. [1-13c]Glucose and [6-13C]glucose were fed to suspensions of yeast cells. Time courses for concentration changes of the starting material, of courses for concentration changes of the starting material, of the intermediate fructos...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： den Hollander JA,Brown TR,Ugurbil K,Shulman RG

更新日期：1979-12-01 00:00:00

abstract：:The tumor microenvironment restrains conventional T cell (Tconv) activation while facilitating the expansion of Tregs. Here we showed that Tregs' advantage in the tumor milieu relies on supplemental energetic routes involving lipid metabolism. In murine models, tumor-infiltrating Tregs displayed intracellular lipid ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pacella I,Procaccini C,Focaccetti C,Miacci S,Timperi E,Faicchia D,Severa M,Rizzo F,Coccia EM,Bonacina F,Mitro N,Norata GD,Rossetti G,Ranzani V,Pagani M,Giorda E,Wei Y,Matarese G,Barnaba V,Piconese S

更新日期：2018-07-10 00:00:00

abstract：:Junctional Adhesion Molecule-A (JAM-A) is a transmembrane adhesive protein expressed at endothelial junctions and in leukocytes. Here we report that JAM-A is required for the correct infiltration of polymorphonuclear leukocytes (PMN) into an inflamed peritoneum or in the heart upon ischemia-reperfusion injury. The def...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Corada M,Chimenti S,Cera MR,Vinci M,Salio M,Fiordaliso F,De Angelis N,Villa A,Bossi M,Staszewsky LI,Vecchi A,Parazzoli D,Motoike T,Latini R,Dejana E

更新日期：2005-07-26 00:00:00

abstract：:Comparisons were made of the T1 ribonuclease digests of 32P-labeled nucleolar 45S RNA of intact Novikoff hepatoma cells and the RNA synthesized in vitro by isolated nucleoli. Approximately 200 oligonucleotide spots were found in the two-dimensional chromatogram of 45S nucleolar RNA labeled in vivo, which includes frag...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ballal NR,Choi YC,Mouche R,Busche H

更新日期：1977-06-01 00:00:00

abstract：:By using a simplified model of small open liquid-like clusters with surface effects, in the gas phase, it is shown how the statistical thermodynamics of small systems can be extended to include metastable supersaturated gaseous states not too far from the gas-liquid equilibrium transition point. To accomplish this, on...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hill TL,Chamberlin RV

更新日期：1998-10-27 00:00:00

abstract：:The primary structure of the Escherichia coli hemolysin polypeptide (HlyA) is used to predict intramolecular structures involved in the secretion and cytolytic activity of the molecule. The C-terminal region of HlyA contains a repeated, 8-amino acid chain represented by the consensus sequence Leu-Xaa-Gly-Gly-Xaa-Gly-A...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Felmlee T,Welch RA

更新日期：1988-07-01 00:00:00

abstract：:The corticotropin-releasing factor (CRF) ligand family has diverse effects on the CNS, including the modulation of the stress response. The ligands' effects are mediated by binding to CRF G protein-coupled receptors. We have determined the 3D NMR structure of the N-terminal extracellular domain (ECD1) of the mouse CRF...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Grace CR,Perrin MH,DiGruccio MR,Miller CL,Rivier JE,Vale WW,Riek R

更新日期：2004-08-31 00:00:00

abstract：:Fanconi anemia (FA) is a recessive disorder characterized by congenital abnormalities, progressive bone-marrow failure, and cancer susceptibility. Cells from FA patients are hypersensitive to agents that produce DNA crosslinks and, after treatment with these agents, have pronounced chromosome breakage and other cytoge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakanishi K,Yang YG,Pierce AJ,Taniguchi T,Digweed M,D'Andrea AD,Wang ZQ,Jasin M

更新日期：2005-01-25 00:00:00

abstract：:Matching ATP:NADPH provision and consumption in the chloroplast is a prerequisite for efficient photosynthesis. In terms of ATP:NADPH ratio, the amount of ATP generated from the linear electron flow does not meet the demand of the Calvin-Benson-Bassham (CBB) cycle. Several different mechanisms to increase ATP availabi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Voon CP,Guan X,Sun Y,Sahu A,Chan MN,Gardeström P,Wagner S,Fuchs P,Nietzel T,Versaw WK,Schwarzländer M,Lim BL

更新日期：2018-11-06 00:00:00

abstract：:Myocytes in the atria contain a prohormone that gives rise to atrial natriuretic peptides (ANP), which have intrinsic hemodynamic regulatory activity. Little is known about mechanisms regulating ANP release. In rats with indwelling catheters, acute blood volume expansion with 5% (wt/vol) dextrose increases the amount ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zamir N,Haass M,Dave JR,Zukowska-Grojec Z

更新日期：1987-01-01 00:00:00

abstract：:Recognition of phosphatidylserine (PS) lipids exposed on the extracellular leaflet of plasma membranes is implicated in both apoptotic cell removal and immune regulation. The PS receptor T cell immunoglobulin and mucin-domain-containing molecule 4 (Tim4) regulates T-cell immunity via phagocytosis of both apoptotic (hi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Tietjen GT,Gong Z,Chen CH,Vargas E,Crooks JE,Cao KD,Heffern CT,Henderson JM,Meron M,Lin B,Roux B,Schlossman ML,Steck TL,Lee KY,Adams EJ

更新日期：2014-04-15 00:00:00

abstract：:Chronic morphine administration is associated with the development of tolerance, both clinically and in animal models. Many assume that tolerance is a continually progressive response to chronic opioid dosing. However, clinicians have long appreciated the ability to manage cancer pain in patients for months on stable ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Xu J,Faskowitz AJ,Rossi GC,Xu M,Lu Z,Pan YX,Pasternak GW

更新日期：2015-01-06 00:00:00

abstract：:In layered materials, a common mode of deformation involves buckling of the layers under tensile deformation in the direction perpendicular to the layers. The instability mechanism, which operates in elastic materials from geological to nanometer scales, involves the elastic contrast between different layers. In a reg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Makke A,Perez M,Lame O,Barrat JL

更新日期：2012-01-17 00:00:00