Interrelationships between Ca2+ and adenylate and guanylate cyclases in the control of platelet secretion and aggregation.

abstract：:Expression of the bovine papillomavirus E2 regulatory protein in human cervical carcinoma cell lines repressed expression of the resident human papillomavirus E6 and E7 oncogenes and within a few days caused essentially all of the cells to synchronously display numerous phenotypic markers characteristic of cells under...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goodwin EC,Yang E,Lee CJ,Lee HW,DiMaio D,Hwang ES

更新日期：2000-09-26 00:00:00

abstract：:Cells respond to mechanical forces whether applied externally or generated internally via the cytoskeleton. To study the cellular response to forces separately, we applied external forces to cells via microfabricated magnetic posts containing cobalt nanowires interspersed among an array of elastomeric posts, which act...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sniadecki NJ,Anguelouch A,Yang MT,Lamb CM,Liu Z,Kirschner SB,Liu Y,Reich DH,Chen CS

更新日期：2007-09-11 00:00:00

abstract：:Erythropoietin regulates the proliferation and differentiation of erythroid precursor cells. Its effect is mediated by the erythropoietin receptor (EPOR), a member of a large family of cytokine receptors. The EPOR gene has recently been cloned, sequenced, and characterized. As shown experimentally, its intracellular C...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de la Chapelle A,Träskelin AL,Juvonen E

更新日期：1993-05-15 00:00:00

abstract：:Little is known about how chromatin folds in its native state. Using optimized in situ hybridization and live imaging techniques have determined compaction ratios and fiber flexibility for interphase chromatin in budding yeast. Unlike previous studies, ours examines nonrepetitive chromatin at intervals short enough to...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bystricky K,Heun P,Gehlen L,Langowski J,Gasser SM

更新日期：2004-11-23 00:00:00

abstract：:Midbrain dopamine (DA) neurons respond to sensory stimuli associated with future rewards. When reward is delivered probabilistically, DA neurons reflect this uncertainty by increasing their firing rates in a period between the sensory cue and reward delivery time. Probability of reward, however, has been externally co...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： de Lafuente V,Romo R

更新日期：2011-12-06 00:00:00

abstract：:Chromosome translocations between Ig (Ig) and non-Ig genes are frequently associated with B-cell lymphomas in humans and mice. The best characterized of these is c-myc/IgH translocation, which is associated with Burkitt's lymphoma. These translocations are caused by activation-induced cytidine deaminase (AID), which p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jankovic M,Robbiani DF,Dorsett Y,Eisenreich T,Xu Y,Tarakhovsky A,Nussenzweig A,Nussenzweig MC

更新日期：2010-01-05 00:00:00

abstract：:In all but the poorest countries of South Asia and Africa, the supply and quality of food will rise to meet the demand. Biotechnology, accelerated by genomics, will create wealth for both producers and consumers by reducing the cost and increasing the quality of food. Famine and malnutrition in the poorest countries m...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Briggs SP

更新日期：1998-03-03 00:00:00

abstract：:A five-helix secondary structural model is proposed for eukaryotic cytoplasmic 5S rRNA. All available sequence data are consistent with this model including those from Chlorella 5S rRNA whose sequence is revised by data included here. Various architectural features of eukaryotic 5S rRNA are summarized in terms of this...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Luehrsen KR,Fox GE

更新日期：1981-04-01 00:00:00

abstract：:The goldfish (Carassius auratus) visual pathway displays continuous growth and plasticity throughout life. Since homeobox genes are important transcriptional regulators in development, we searched for homeobox genes in the adult goldfish retina and brain. Using the PCR, we discovered a repertoire of homeobox sequences...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Levine EM,Schechter N

更新日期：1993-04-01 00:00:00

abstract：:Low-temperature flash photolysis with IR and visible spectroscopy was used to probe the influence of the distal histidine His-64(E7) of sperm-whale myoglobin (Mb) on the orientation of bound carbon monoxide (CO) and on the kinetics of CO rebinding. The synthesis and high-level expression of a sperm-whale myoglobin gen...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Braunstein D,Ansari A,Berendzen J,Cowen BR,Egeberg KD,Frauenfelder H,Hong MK,Ormos P,Sauke TB,Scholl R

更新日期：1988-11-01 00:00:00

abstract：:BDF1 [(C57BL/6 X DBA/2)F1] mice were primed with alum-absorbed ovalbumin, and their spleen cells were cultured with ovalbumin to activate antigen-primed T cells. The activated T cells were then propagated in interleukin 2-containing medium in the presence or absence of affinity-purified glycosylation-inhibiting factor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Iwata M,Ishizaka K

更新日期：1987-04-01 00:00:00

abstract：:Type IV pili (Tfp), which are key virulence factors in many bacterial pathogens, define a large group of multipurpose filamentous nanomachines widespread in Bacteria and Archaea. Tfp biogenesis is a complex multistep process, which relies on macromolecular assemblies composed of 15 conserved proteins in model gram-neg...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Goosens VJ,Busch A,Georgiadou M,Castagnini M,Forest KT,Waksman G,Pelicic V

更新日期：2017-06-20 00:00:00

abstract：:Acute myeloid leukemia subtype M4 with eosinophilia is associated with a chromosome 16 inversion that creates a fusion gene CBFB-MYH11. We have previously shown that CBFB-MYH11 is necessary but not sufficient for leukemogenesis. Here, we report the identification of genes that specifically cooperate with CBFB-MYH11 in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castilla LH,Perrat P,Martinez NJ,Landrette SF,Keys R,Oikemus S,Flanegan J,Heilman S,Garrett L,Dutra A,Anderson S,Pihan GA,Wolff L,Liu PP

更新日期：2004-04-06 00:00:00

abstract：:Volcanic eruptions caused major weather and climatic changes on timescales ranging from hours to centuries in the past. Volcanic particles are injected in the atmosphere both as primary particles rapidly deposited due to their large sizes on time scales of minutes to a few weeks in the troposphere, and secondary parti...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boulon J,Sellegri K,Hervo M,Laj P

更新日期：2011-07-26 00:00:00

abstract：:To investigate protein folding dynamics in terms of compactness, we developed a continuous-flow mixing device to make small-angle x-ray scattering measurements with the time resolution of 160 micros and characterized the radius of gyration (R(g)) of two folding intermediates of cytochrome c (cyt c). The early intermed...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Akiyama S,Takahashi S,Kimura T,Ishimori K,Morishima I,Nishikawa Y,Fujisawa T

更新日期：2002-02-05 00:00:00

abstract：:Mitochondria isolated from maize plants with S-type male-sterile cytoplasms are capable of synthesizing four species of RNA at concentrations of actinomycin D that eliminate all DNA-directed RNA synthesis. No RNA synthesis occurs under the same conditions with mitochondria from plants possessing normal (N) cytoplasm o...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Finnegan PM,Brown GG

更新日期：1986-07-01 00:00:00

abstract：:The anti-inflammatory activity of intravenous Ig (IVIG) results from a minor population of the pooled IgG molecules that contains terminal alpha2,6-sialic acid linkages on their Fc-linked glycans. These anti-inflammatory properties can be recapitulated with a fully recombinant preparation of appropriately sialylated I...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anthony RM,Wermeling F,Karlsson MC,Ravetch JV

更新日期：2008-12-16 00:00:00

abstract：:The aryl hydrocarbon receptor (AhR) knockout mice raised in the laboratory of Fujii-Kuriyama have been under investigation for several years because of the presence in their urinary bladder of large, yellowish stones. The stones are composed of uric acid and become apparent in the bladders as tiny stones when mice are...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Butler R,Inzunza J,Suzuki H,Fujii-Kuriyama Y,Warner M,Gustafsson JÅ

更新日期：2012-01-24 00:00:00

abstract：:The covalent attachment of heme to apocytochrome c, and therefore the import of cytochrome c into mitochondria, is dependent on both NADH plus a cytosolic cofactor that has been identified to be FMN or FAD. NADH in concert with flavin nucleotides mediates the reduction of heme. Heme in the reduced state is a prerequis...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nicholson DW,Neupert W

更新日期：1989-06-01 00:00:00

abstract：:Observations on the structure and function of the ear in amphisbaenians have been extended to two new species: to Blanus cinereus of the family Amphisbaenidae and Diplometopon zarudnyi of the family Trogonophidae. Blanus, considered one of the most primitive of this group of reptiles, shows a distinctive form of sound...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gans C,Wever EG

更新日期：1975-04-01 00:00:00

abstract：:Multiple protein subcomplexes of the kinetochore cooperate as a cohesive molecular unit that forms load-bearing microtubule attachments that drive mitotic chromosome movements. There is intriguing evidence suggesting that central kinetochore components influence kinetochore-microtubule attachment, but the mechanism re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kudalkar EM,Scarborough EA,Umbreit NT,Zelter A,Gestaut DR,Riffle M,Johnson RS,MacCoss MJ,Asbury CL,Davis TN

更新日期：2015-10-13 00:00:00

abstract：:Oligosaccharide moieties of cell-surface glycoconjugates are thought to be involved in recognition events associated with tumor metastasis and invasion. Using swainsonine (SW), an inhibitor of Golgi alpha-mannosidase II that results in the formation of hybrid-type oligosaccharides on N-linked glycoproteins, we have te...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Humphries MJ,Matsumoto K,White SL,Olden K

更新日期：1986-03-01 00:00:00

abstract：:Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reco...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Armache JP,Jarasch A,Anger AM,Villa E,Becker T,Bhushan S,Jossinet F,Habeck M,Dindar G,Franckenberg S,Marquez V,Mielke T,Thomm M,Berninghausen O,Beatrix B,Söding J,Westhof E,Wilson DN,Beckmann R

更新日期：2010-11-16 00:00:00

abstract：:Pure cultures of human breast epithelial cells and of fibroblastic cells in early passage provided the opportunity to ask whether either cell type had the capability for metabolizing chemical carcinogens and, if so, was the fate of the metabolic products compatible with chemical carcinogens being a factor in the initi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stampfer MR,Bartholomew JC,Smith HS,Bartley JC

更新日期：1981-10-01 00:00:00

abstract：:Cells are nonequilibrium systems that exchange matter and energy with the environment to sustain their metabolic needs. The nonequilibrium nature of this system presents considerable challenges to developing a general theory describing its behavior; however, when studied at appropriate spatiotemporal scales, the behav...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang AY,Marshall WF

更新日期：2019-10-22 00:00:00

abstract：:The Xlim-1 gene is activated in the late blastula stage of Xenopus embryogenesis in the mesoderm, and its RNA product becomes concentrated in the Spemann organizer at early gastrula stage. A major regulator of early expression of Xlim-1 is activin or an activin-like signal. We report experiments aiming to identify the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rebbert ML,Dawid IB

更新日期：1997-09-02 00:00:00

abstract：:The sensitive and accurate quantification of protein biomarkers plays important roles in clinical diagnostics and biomedical research. Sandwich ELISA and its variants accomplish the capture and detection of a target protein via two antibodies that tightly bind at least two distinct epitopes of the same antigen and hav...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chatterjee T,Knappik A,Sandford E,Tewari M,Choi SW,Strong WB,Thrush EP,Oh KJ,Liu N,Walter NG,Johnson-Buck A

更新日期：2020-09-15 00:00:00

abstract：:Influenza virus infections cause a wide variety of outcomes, from mild disease to 3 to 5 million cases of severe illness and ∼290,000 to 645,000 deaths annually worldwide. The molecular mechanisms underlying these disparate outcomes are currently unknown. Glycosylation within the human host plays a critical role in in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Heindel DW,Koppolu S,Zhang Y,Kasper B,Meche L,Vaiana CA,Bissel SJ,Carter CE,Kelvin AA,Elaish M,Lopez-Orozco J,Zhang B,Zhou B,Chou TW,Lashua L,Hobman TC,Ross TM,Ghedin E,Mahal LK

更新日期：2020-10-27 00:00:00

abstract：:Although gamma frequency oscillations are common in the brain, their functional contributions to neural computation are not understood. Here we report in vitro electrophysiological recordings to evaluate how noisy gamma frequency oscillatory input interacts with the overall activation level of a neuron to determine th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Markowitz DA,Collman F,Brody CD,Hopfield JJ,Tank DW

更新日期：2008-06-17 00:00:00

abstract：:Both laboratory and field data suggest that people punish noncooperators even in one-shot interactions. Although such "altruistic punishment" may explain the high levels of cooperation in human societies, it creates an evolutionary puzzle: existing models suggest that altruistic cooperation among nonrelatives is evolu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Boyd R,Gintis H,Bowles S,Richerson PJ

更新日期：2003-03-18 00:00:00