Abstract:
:The continuous human promyeloid cell line HL60 may be induced to differentiate into neutrophils by the presence of 1.25% dimethylsulphoxide (DMSO) and related compounds [4]. When treated with 12-O-tetradecanoylphorbol-13-acetate (TPA), the cells exhibit may features characteristic of monocytes/macrophages [13]. In both cases, the induced HL60 cells lose their proliferative ability. The availability of this inducible cell line has led to an increasing number of studies aimed at dissecting the process of myeloid differentiation. However, the correct interpretation of such studies may only be reached through the use of appropriate cellular controls; specifically, variant HL60 cell lines which do not differentiate in response to the inducing agents. Here we report the isolation and characterization of two variant cell lines of HL60 which do not differentiate in the presence of 1.25% dimethylsulphoxide but still respond to TPA. Comparison of these HL60 variants with the parental line should facilitate the elucidation of molecular events which regulate myeloid differentiation.
journal_name
Leuk Resjournal_title
Leukemia researchauthors
Toksoz D,Bunce CM,Stone PC,Michell RH,Brown Gdoi
10.1016/0145-2126(82)90006-6subject
Has Abstractpub_date
1982-01-01 00:00:00pages
491-8issue
4eissn
0145-2126issn
1873-5835journal_volume
6pub_type
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journal_title:Leukemia research
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journal_title:Leukemia research
pub_type: 杂志文章,评审
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journal_title:Leukemia research
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journal_title:Leukemia research
pub_type: 杂志文章
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pub_type: 杂志文章,多中心研究
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journal_title:Leukemia research
pub_type: 杂志文章
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更新日期:2007-05-01 00:00:00
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pub_type: 临床试验,杂志文章
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更新日期:2003-04-01 00:00:00
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pub_type: 临床试验,杂志文章
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pub_type: 杂志文章
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journal_title:Leukemia research
pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Leukemia research
pub_type: 杂志文章
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journal_title:Leukemia research
pub_type: 杂志文章
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更新日期:2006-10-01 00:00:00