Abstract:
:The activities of monoamine oxidase (MAO), catechol-O-methyltransferase (COMT) and gamma-aminobutyric acid transaminase (GABA-T) were measured in primary cultures from newborn rat cultivated from 6 different brain regions. These primary cultures contained mostly astroglial cells, evaluated by the presence of the glial fibrillary acidic protein (GFAp, alpha-albumin) and the S-100 protein. The enzyme activities in the corresponding brain area from adult rat were also quantified. MAO activities were on the same level in 14-day old cultures and in adult rat brain homogenates, with significantly lower values in brain stem as compared to the other brain regions examined. COMT activities were on a higher level in the cultures than in adult rat brain homogenates. Astroglial cells from hippocampus were found to have the highest and those from brain stem the lowest COMT-activities. GABA-T activities were lower in the cultures than in adult rat homogenates. No significant differences were seen in the various astroglial cultures. Accumulation of [3H]dopamine and [3H]gamma-aminobutyric acid (GABA) visualized by autoradiography showed only a slight uptake of dopamine in comparison with the uptake of GABA. It is concluded that astroglial cells in culture have enzymatic properties similar to those of astroglial cells in different brain regions of adult rat brain. Studies are in progress to evaluate if the regional heterogeneity observed among cultivated astroglial cells is affected by in vivo differentiation until cultivation and/or time in culture.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Hansson Edoi
10.1007/BF00967658subject
Has Abstractpub_date
1984-01-01 00:00:00pages
45-57issue
1eissn
0364-3190issn
1573-6903journal_volume
9pub_type
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更新日期:2013-03-01 00:00:00
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更新日期:2016-09-01 00:00:00
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journal_title:Neurochemical research
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journal_title:Neurochemical research
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更新日期:1984-02-01 00:00:00
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更新日期:2003-09-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章,评审
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更新日期:2004-05-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:1993-02-01 00:00:00
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更新日期:2018-01-01 00:00:00
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更新日期:2014-11-01 00:00:00
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更新日期:2012-12-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章,评审
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更新日期:1996-11-01 00:00:00
abstract::Proteolipid protein (PLP) is the major intrinsic membrane protein of CNS myelin and is expressed in oligodendrocytes as part of a coordinate program of myelin-specific gene activation. In order to identify the DNA sequences and proteins involved in the regulation of PLP transcription, we have analyzed the 5' flanking ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00968716
更新日期:1994-08-01 00:00:00
abstract::We observed a spot on two-dimensional (2-D) gel in the epileptic mutant strain E1 mice with a similar molecular weight but with a different isoelectric point of approximately 0.2, compared with its mother strain ddY mice. The collected protein from the E1 mice was identified as cytosolic NADP+-dependent isocitrate deh...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020981532281
更新日期:1999-03-01 00:00:00
abstract:UNLABELLED:Depressive disorder is a multifactorial diseases, that one of the typical feature are cognitive impairments. The aim of this study was to determine the total antioxidant status (TAS) in patients with recurrent depressive disorder (rDD) and to define relationship between plasma levels of TAS and the cognitive...
journal_title:Neurochemical research
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doi:10.1007/s11064-012-0788-z
更新日期:2012-08-01 00:00:00
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doi:10.1007/s11064-018-2544-5
更新日期:2018-06-01 00:00:00
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journal_title:Neurochemical research
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更新日期:1998-10-01 00:00:00
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journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969081
更新日期:1993-03-01 00:00:00
abstract::The wobbler mouse suffers an autosomal recessive mutation producing severe neurodegeneration and astrogliosis in spinal cord. It has been considered a model for amyotrophic lateral sclerosis. We have studied in these animals the expression of two proteins, the growth-associated protein (GAP-43) and the NADPH-diaphoras...
journal_title:Neurochemical research
pub_type: 杂志文章
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更新日期:1999-01-01 00:00:00