Abstract:
:Diisopropyl phosphorofluoridate (DFP) produces type I organophosphorus ester-induced delayed neurotoxicity in humans and sensitive animal species. This is accompanied by enhanced Ca2+/CaM-dependent protein kinase II (CaM-kinase II) activity, and [125I]calmodulin binding to CaM-kinase II in DFP-treated hen brain supernatant without increase in the enzyme quantity. We have purified CaM-kinase II from control and DFP-treated hen whole brains and compared various physical and biochemical properties. The two enzymes exhibited similar properties in many respects. However, there was a decrease in calcium-independent protein kinase II activity after autophosphorylation, and an increase in K0.5 for free calcium and calmodulin of enzyme purified from DFP-treated hen brains. This change in kinetic parameters may result in greater percentage of total CaM-kinase II present in unphosphorylated form, which is consistent with the increased autophosphorylation of CaM-kinase II and [125I]calmodulin binding in the brain supernatant of DFP-treated hens.
journal_name
Neurochem Resjournal_title
Neurochemical researchauthors
Gupta RP,Abou-Donia MBdoi
10.1007/BF00969081subject
Has Abstractpub_date
1993-03-01 00:00:00pages
259-69issue
3eissn
0364-3190issn
1573-6903journal_volume
18pub_type
杂志文章abstract::1-Methyl-4-phenylpyridinium (MPP(+)) or 6-hydroxydopamine (6-OHDA) caused a nuclear damage, the mitochondrial membrane permeability changes, leading to the cytochrome c release and caspase-3 activation, the formation of reactive oxygen species and the depletion of GSH in PC12 cells. Nicardipine (a calcium channel bloc...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-006-9088-9
更新日期:2006-07-01 00:00:00
abstract::The purpose of this study was to investigate whether the kappa-opioid receptor (KOR) agonist, BRL52537, has a neuroprotective effect against cerebral ischemia/reperfusion (I/R) injury in rats and further explore the underlying mechanisms. Adult male Sprague-Dawley rats were randomly assigned into sham (group A), I/R (...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1139-4
更新日期:2013-11-01 00:00:00
abstract::Evidence has suggested that cerebrospinal fluid-contacting nucleus (CSF-contacting nucleus) is correlated with the development and recurrence of pain. A recent research showed that the CSF-contacting nucleus acts as a component of the descending 5-hydroxytryptamine (5-HT) system and plays a role in descending pain inh...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-1880-6
更新日期:2016-07-01 00:00:00
abstract::Müller cells, the predominant glial cells in the retina, are thought to play important roles in the survival of retinal neurons. Previous studies have demonstrated that Müller cells express brain-derived neurotrophic factor (BDNF), which has a pronounced neurotrophic effect on retinal ganglion cells. In this study, we...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-005-7936-7
更新日期:2005-09-01 00:00:00
abstract::We analyzed the impact of axonopathy-inducing agents 1,2-diacetylbenzene (1,2-DAB) and 2,5-hexanedione (2,5-HD) on membrane-bound protein disulfide isomerase (mPDI) versus soluble PDI (sPDI), or PDI-family member thioredoxin (THX), and asked whether changes in PDI/THX were associated with production of oxidative/nitro...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-009-9977-9
更新日期:2009-11-01 00:00:00
abstract::The real-time iontophoretic method has measured volume fraction and tortuosity of the interstitial component of extracellular space in many regions and under different conditions. To interpret these data computer models of the interstitial space (ISS) of the brain are constructed by representing cells as Basic Cellula...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-019-02793-6
更新日期:2020-01-01 00:00:00
abstract::The role of protein phosphorylation in catecholamine secretion from bovine adrenomedullary chromaffin cells was studied using different protein kinase inhibitors. Naphthalenesulfonamide derivatives as ML9 and ML7, more specific for the myosin light chain kinase, and the calmodulin antagonist W7 inhibited catecholamine...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00969089
更新日期:1993-03-01 00:00:00
abstract::Many studies have demonstrated that apoptosis play an important role in cerebral ischemic pathogenesis and may represent a target for treatment. Neuroprotective effect of quercetin has been shown in a variety of brain injury models including ischemia/reperfusion. It is not clear whether BDNF-TrkB-PI3K/Akt signaling pa...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-012-0871-5
更新日期:2012-12-01 00:00:00
abstract::Deposition of amyloid beta peptide in human brain in the form of senile plaques is a neuropathological hallmark of Alzheimer's disease (AD). Levels of a phospholipid breakdown product, glycerophosphocholine (GPC), also increase in AD brain. The effect of GPC on amyloid beta(1-40) peptide (Abeta) aggregation in PBS buf...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-004-7036-0
更新日期:2004-12-01 00:00:00
abstract::Brief limb ischemia was reported to protect neurons against injury induced by subsequent cerebral ischemia-reperfusion, and this phenomenon is known as limb ischemic preconditioning (LIP). To explore the role of nitric oxide (NO) in neuroprotection of LIP in rats, we observed changes in the content of nitric oxide (NO...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9381-2
更新日期:2007-11-01 00:00:00
abstract::Selective blockade of a subset of GABA(A) receptors may be involved in the antipsychotic effects of Clozapine and several other antipsychotic drugs. Seven antipsychotic drugs, and 11 drugs classified as antidepressants that only partially reverse the inhibitory effect of 1 microM GABA on [35S]TBPS binding, do not yiel...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1027359422433
更新日期:1997-02-01 00:00:00
abstract::Serotonin uptake and metabolism was studied in ependymal primary cultures. Serotonin uptake was facilitated by two different systems, one of which was the neuronal serotonin transporter SERT, exhibiting a Vmax value of 3.8+/-0.1 pmol x min(-1) x (mg protein)(-1) and an apparent Michaelis-Menten constant of 0.41+/-0.03...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000035810.08543.97
更新日期:2004-09-01 00:00:00
abstract::Neuronal excitation leads to an increase of the extracellular K(+) concentration ([K(+)]o) in brain. This increase has at least two energy-consuming consequences: (1) a depolarization-mediated change in intracellular pH (pHi) in astrocytes due to depolarization-mediated increased activity of the acid-extruding Na(+)/b...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-013-1149-2
更新日期:2013-11-01 00:00:00
abstract::The effects of phenylalanine (PHE) and its deaminated metabolites phenylpyruvate (PHP), phenyllactate (PHL) and phenylacetate (PHA) on sodium and potassium activated adenosinetriphosphatase (Na+,K+-ATPase) in synaptosomes from rat brain were investigated. At very low concentrations (5-10 microM). PHE, PHL and PHA inhi...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00965524
更新日期:1982-06-01 00:00:00
abstract::The volume-regulated anion channel, VRAC, plays an important role in cell volume regulation. This channel is permeable for a wide variety of anions, amino acids, and organic osmolytes, including taurine. However, nothing is known about possible water permeability of this channel. Water permeability of endothelial cell...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/b:nere.0000010430.23137.be
更新日期:2004-01-01 00:00:00
abstract::A cortical cup model with continuous perfusion of artificial cerebrospinal fluid (containing 134 mM NaCl) was used to investigate the effects of anion channel blockers on the hyposmotically-induced release of amino acids from the in vivo rat cerebral cortex. The hyposmotic stimulus (25 mM NaCl) evoked a release of tau...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020902104056
更新日期:1999-03-01 00:00:00
abstract::Experimentalists would like to perform large-scale expression analysis on samples drawn from small cell populations and even single cells. Amplification of the source material is required to assay such small samples under all existing array technologies. In this report we consider the very different theoretical proper...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1023/a:1020917223962
更新日期:2002-10-01 00:00:00
abstract::One pathway by which tryptophan is metabolized in the brain as well as in the periphery is through cleavage of the indole ring to formylkynurenine and then kynurenine. Indoleamine-2,3-dioxygenase, the enzyme that catalyzes this cleavage, and kynurenine are distributed all across the different anatomic regions of brain...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/BF00964611
更新日期:1980-03-01 00:00:00
abstract::The long-acting opiate antagonist naltrexone hydrochloride was administered by intraperitoneal injection, in a dose response protocol, to adult rats. The drug was used to observe effects of opiate receptor blockade on cells of the pituitary gland and adjacent hypothalamus. At higher drug doses (5 mg/kg or 10 mg/kg), n...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964168
更新日期:1984-02-01 00:00:00
abstract::The neurotoxic effects of aluminum are generally associated with reduced antioxidant capacity, increased oxidative stress and apoptosis, which lead to the induction of neurodegenerative processes. Curcumin has a lipophilic polyphenol character and effects of antioxidant and anti-apoptotic. The present study was undert...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-019-02839-9
更新日期:2019-08-01 00:00:00
abstract::The effects of acute lorazepam challenges on plasma (p) HVA, MHPG, and 5-HIAA, and their relationship to drug-induced cognitive and motor deficits and the apolipoprotein (APOE)-epsilon4 allele were examined. Eighteen healthy elderly (8 epsilon4 carriers) received placebo or acute oral lorazepam doses (0.5 mg or 1 mg) ...
journal_title:Neurochemical research
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1023/b:nere.0000026402.09466.54
更新日期:2004-07-01 00:00:00
abstract::Propofol and ketamine may provide certain degree of neuroprotection, but the underlying mechanism remains unclear to date. The cAMP response element-binding protein (CREB) was proposed that its phosphorylation at Ser133 (P-CREB) constituted a convergence point involved in neuroprotection. The purpose of this study was...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-011-0582-3
更新日期:2012-01-01 00:00:00
abstract::Kv1.3 is a voltage gated potassium channel that has been implicated in pathophysiology of multiple sclerosis (MS). In the present study we investigated temporal and cellular expression pattern of this channel in the lumbar part of spinal cords of animals with experimental autoimmune encephalomyelitis (EAE), animal mod...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-018-2509-8
更新日期:2018-05-01 00:00:00
abstract::Parkinson's disease (PD) is a neurodegenerative disorder of dopaminergic, noradrenergic, and serotonergic systems, in which dopamine, noradrenaline, and serotonin levels are depleted and lead to the development of motor and non-motor symptoms such as tremor, bradykinesia, weight changes, fatigue, depression, and visua...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-020-03130-y
更新日期:2020-11-01 00:00:00
abstract::We report here the isolation of fractions enriched in components of the myelin-like membranes surrounding the giant axons of the earthworm. Lumbricus terrestris L. The composition and purity of the fractions have been assessed using SDS-protein electrophoresis, Western immunoblots, and electron microscopy. Preliminary...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00970759
更新日期:1988-09-01 00:00:00
abstract::Due to the biological importance of sodium and its relative scarcity within many natural environments, 'salt appetite' has evolved whereby dietary salt is highly sought after and palatable when tasted. In addition to peripheral responses, salt depletion is detected within the brain via circumventricular organs and 11β...
journal_title:Neurochemical research
pub_type: 杂志文章,评审
doi:10.1007/s11064-017-2336-3
更新日期:2018-01-01 00:00:00
abstract::The human formyl peptide receptor like 1 (FPRL-1) is a variant of the Gi-coupled formyl-peptide receptor. Functional FPRL-1 is endogenously expressed in the U87 astrocytoma cell line and there is accumulating evidence to suggest that FPRL-1 may be involved in neuroinflammation associated with the pathogenesis of Alzhe...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-007-9425-7
更新日期:2008-01-01 00:00:00
abstract::Despite the consensus that activation of TWIK-related spinal cord K+ (TRESK) might contribute to the pathogenesis of chronic pain, the specific mechanisms underlying the transfer and development of pain signals still remain obscure. In the present study, we validated that TRESK was expressed in neurons instead of glia...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/s11064-016-2170-z
更新日期:2017-05-01 00:00:00
abstract::The folate cycle, which has a close correlation with nucleic acid synthesis as well as with sulfur amino acid metabolism, may have some bearing on the pathogenesis of amyotrophic lateral sclerosis. The folate cycle hypothesis offers a unified explanation of the reduction of RNA and the elevation of taurine in nervous ...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00963985
更新日期:1984-03-01 00:00:00
abstract::Detergents Triton X-100, sodium deoxycholate, and octyl-beta-D-glucopyranoside, and proteinase papain proved to be excellent agents solubilizing the gamma-glutamyltransferase (gamma-GT) from human brain cortex microvessels. Ficin also solubilized gamma-GT but to a lesser extent than papain. The relative molecular mass...
journal_title:Neurochemical research
pub_type: 杂志文章
doi:10.1007/BF00964524
更新日期:1984-07-01 00:00:00