Abstract:
:Primary hepatocyte cultures from six human donors were established and their abilities to metabolize the polycyclic aromatic hydrocarbon carcinogen benzo[a]pyrene (B[a]P) were examined. Cells from each donor were plated at similar densities (1 X 10(7) cells/100 mm dish). All cultures metabolized B[a]P to a significant extent (24-35 nmol in 24 h) and h.p.l.c. profiles of the organic solvent-soluble and glucuronidated B[a]P metabolites were obtained for all donors. The predominant extracellular organic solvent-soluble B[a]P metabolites were the 9,10- and 7,8-dihydrodiols, 9-hydroxy-B[a]P, and a mixture of tetrols, but the general ratios of these metabolites varied widely among the cells from different donors. In contrast, profiles were highly reproducible in cells from the same donor treated with B[a]P at either 8 or 24 h after initial plating. There was less variability in the amounts of specific B[a]P metabolites conjugated to glucuronic acid by cells from various donors. This variability could not be correlated with cell viability or overall levels of B[a]P metabolism. In addition, B[a]P metabolism by fresh and cryopreserved hepatocytes from the same donor was compared. While there was only a small reduction in the level of total B[a]P metabolism after cell freezing, there was a 3- to 5-fold increase in production of B[a]P-7,8-dihydrodiol, found both in the extracellular medium and as glucuronic acid conjugates, by the cryopreserved cells tested.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Moore CJ,Gould MNdoi
10.1093/carcin/5.12.1577subject
Has Abstractpub_date
1984-12-01 00:00:00pages
1577-82issue
12eissn
0143-3334issn
1460-2180journal_volume
5pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::Treatment of pregnant mice with 3-methylcholanthrene (MC) causes lung and liver tumors in the offspring, the incidences of which are greatly influenced by the Ah locus regulated induction phenotype for aryl hydrocarbon hydroxylase activity (AHH) in both the mother and fetuses. In order to examine the biochemical and m...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.11.1979
更新日期:1990-11-01 00:00:00
abstract::The objective was to study the relationship between cyclin D1 gene (CCND1) polymorphism and lung cancer in the Chinese population. Blood samples of 182 cases and 185 controls were collected from a hospital based case-control study. PCR-SSCP was used to examine the G/A polymorphism in exon 4 of CCND1. The results showe...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgg035
更新日期:2003-09-01 00:00:00
abstract::Since the results of an earlier study indicating no effect of dietary fat on dimethylhydrazine (DMH)-induced colon cancer in rats differed from those of other investigators, the present study was initiated to determine if the modulating effect of fat intake on colon tumorigenesis was dependent on the route of DMH admi...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.3.349
更新日期:1985-03-01 00:00:00
abstract::Recent studies have proposed that exposure to ultraviolet radiation (UVR) protects against development of some internal cancers including that in prostate. This effect may be mediated by UVR-induced cutaneous synthesis of vitamin D. It is also proposed that ability to pigment in response to UVR will influence suscepti...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgg021
更新日期:2003-04-01 00:00:00
abstract::Genotoxic carcinogens, including 2-acetylaminofluorene (2-AAF), in addition to exerting their genotoxic effects, often cause a variety of non-genotoxic alterations in cells. It is believed that these non-genotoxic effects may be indispensable events in tumorigenesis; however, there is insufficient knowledge to clarify...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgm303
更新日期:2008-03-01 00:00:00
abstract::Genetically modified mice with overexpressed and/or deleted genes have been used extensively to model human cancer. However, it is uncertain as to what extent the mouse models reproduce the corresponding cancers in humans. We have compared the global gene expression patterns in human and mouse hepatocellular carcinoma...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi030
更新日期:2005-06-01 00:00:00
abstract::Induction of glutathione S-transferase (GST) enzymes can increase detoxification of carcinogens and reduce carcinogen-induced mutagenesis and tumorigenesis. To determine if the anticarcinogen ellagic acid induces cellular enzymes which detoxify carcinogens, we examined the effect of ellagic acid on the expression of g...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.3.665
更新日期:1995-03-01 00:00:00
abstract::2-Amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a mammary gland carcinogen present in the human diet. Herein, the expression of estrogen receptor alpha (ERalpha), estrogen receptor beta (ER beta) and progesterone receptor (PR) was examined in mammary gland carcinomas induced by PhIP in female Sprague-Dawley ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi013
更新日期:2005-04-01 00:00:00
abstract::Common polymorphisms in genes encoding phase I and phase II enzymes are considered to modify lung cancer risk due to changes in enzyme activity. Candidates include genetic variants of glutathione S-transferases (GSTM1, GSTT1 and GSTP1) and myeloperoxidase (MPO). We performed a large case-control study of these candida...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi227
更新日期:2006-03-01 00:00:00
abstract::In order to test the controversially discussed hypothesis that oval cells are part of a liver stem cell compartment and can give rise to cholangiocellular as well as hepatocellular carcinomas in the course of liver carcinogenesis, we transfected an oval cell line established in our laboratory with an oncogenically act...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.12.2635
更新日期:1996-12-01 00:00:00
abstract::Resveratrol is a phytochemical that has been under consideration for use as a prostate cancer chemopreventive agent. However, the efficacy, as well as the mechanisms of action of resveratrol on prostate cancer prevention, remains largely unknown. This study seeks to address these questions and examine the cancer preve...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn131
更新日期:2008-10-01 00:00:00
abstract::Purified DNA was modified in vitro by 3H-labelled O-acetyl or O,O'-diacetyl-4-hydroxyaminoquinoline-1-oxide (Ac4HAQO or di Ac-4HAQO). It was then subjected to the action of the single-stranded DNA specific nuclease S1 and the digested fractions were analysed. For both types of modified DNA, the release of non-modified...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/3.4.435
更新日期:1982-01-01 00:00:00
abstract::Heterocyclic aromatic amines (HAAs), formed during the cooking of foods, are known to induce tumours in rodent bioassays and may thus contribute to human cancer risk. We tested six HAAs in a morphological transformation assay and in three in vitro genotoxicity assays. The morphological transforming abilities of HAAs w...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/20.4.545
更新日期:1999-04-01 00:00:00
abstract::Folate and vitamin D have been shown to be influenced by ultraviolet (UV) radiation. UVA radiation can break down plasma folate, whereas vitamin D can be synthesized in UVB-exposed skin. Folate metabolism is involved in DNA synthesis and repair, and vitamin D processes anti-proliferative effects. The functions of both...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgl156
更新日期:2007-02-01 00:00:00
abstract::We have investigated the role of the signaling intermediate, EPS8, in tumor progression using a model system and in vivo. HN4 primary tumor cells express low levels of EPS8, similar to normal keratinocytes, and show minimal invasion in vitro in response to epidermal growth factor, whereas HN12 cells express high level...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn252
更新日期:2009-01-01 00:00:00
abstract::Breast cancer is the most common cancer and the second most frequent cause of cancer death in women. Despite extensive research, the precise mechanisms of breast carcinogenesis remain unclear. One of the reasons for this is due, at least in part, to a lack of a suitable animal model which can closely mimic the breast ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/20.6.1069
更新日期:1999-06-01 00:00:00
abstract::The activity of nuclear transcription factors is often regulated by specific kinase-signaling pathways. We have previously shown that the organochlorine pesticide dichlorodiphenyltrichloroethane (DDT) stimulates activator protein-1 activity through the p38 mitogen-activated protein kinase (MAPK). Here, we show that DD...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn213
更新日期:2009-01-01 00:00:00
abstract::Methylation of the non-carcinogen 15,16-dihydrocyclopenta[a]phenanthren-17-one (CPP-17-one) at the bay region to form 11-CH3-CPP-17-one confers carcinogenic potential. In the present study we have investigated the in vitro metabolism and mutagenicity of the methylated compound by hepatic microsomal preparations from r...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.9.1783
更新日期:1993-09-01 00:00:00
abstract::In order to address the hypothesis that 6-aminochrysene (6-AC) is converted to genotoxic products by cytochrome P450 enzymes via two activation pathways (N-hydroxylation and epoxidation), the activation of 6-AC and trans-1,2-dihydro-1,2-dihydroxy-6-aminochrysene (6-AC-diol) to genotoxic metabolites was examined in rat...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.3.465
更新日期:1994-03-01 00:00:00
abstract::3,3'-Diindolylmethane (DIM), an indole derivative produced on consumption of broccoli and other cruciferous vegetables, has been shown to have multiple anticancer effects in both in vivo and in vitro models. The present study was carried out to clarify the mechanism of DIM's antiangiogenic activity. We found that DIM ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi230
更新日期:2006-03-01 00:00:00
abstract::DNA-demethylating agents activate tumor suppressor genes that are silenced by DNA methylation in cancer and are therefore emerging as a novel approach to cancer therapy. 5-azacytidine (VIDAZA), the first representative of this class of drugs was approved for treatment of myelodysplastic syndromes and is currently bein...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgt284
更新日期:2014-01-01 00:00:00
abstract::We conducted a cross-sectional molecular epidemiological study of coke oven workers exposed to the established carcinogen polycyclic aromatic hydrocarbons (PAHs) to evaluate the relationships between both traditional 'exposure markers' and a series of biomarkers, including urinary 1-hydroxypyrene as a marker of intern...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.11.1963
更新日期:1998-11-01 00:00:00
abstract::The effect of the aromatic amines 2-acetylaminofluorene (AAF), 2-acetylaminophenanthrene (AAP) and trans-4-acetylaminostilbene (AAS) on the rat hepatic aromatic hydrocarbon (Ah) receptor level was studied. 3-Methylcholanthrene (MC), as a known receptor ligand, was used as a control. The complete liver carcinogen AAF a...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.7.1021
更新日期:1987-07-01 00:00:00
abstract::The arylamine carcinogen 3,2'-dimethyl-4-aminobiphenyl (DMABP) has been proposed to be metabolically activated to DNA-binding derivatives through the formation of an N-hydroxy intermediate. In this study, the subsequent activation of N-hydroxy-DMABP through acid catalysis or enzymatic esterification was examined. [Rin...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.2.251
更新日期:1985-02-01 00:00:00
abstract::Nitric oxide (NO) is a short-lived, pleiotropic molecule that affects numerous critical functions in the body. Presently, there are markedly conflicting findings in the literature regarding NO and its role in carcinogenesis and tumor progression. NO has been shown to have dichotomous effects on cellular proliferation,...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgt034
更新日期:2013-03-01 00:00:00
abstract::Whole-body autoradiography of [3H]aflatoxin B1 ([3H]AFB1) in lamb showed a localization of bound labelling, in addition to the liver, in the nasal olfactory and respiratory mucosa, in the mucosa of the nasopharynx, pharynx, oesophagus, larynx, trachea, bronchi and bronchioles and in the palpebral and bulbar conjunctiv...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.5.947
更新日期:1994-05-01 00:00:00
abstract::Lack of functional telomeres can cause chromosomal aberrations. This type of genetic instability may promote tumorigenesis. We have investigated the association between mean telomere length in buccal cells (assessed with quantitative real-time PCR) and bladder cancer risk in a case-control study. Patients with bladder...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi063
更新日期:2005-07-01 00:00:00
abstract::Cancer testis antigens (CTAs) are promising targets for T cell-based immunotherapy and studies have shown that certain CT genes are epigenetically depressed in cancer cells through DNA demethylation. Melanoma-associated antigen A11 (MAGE-A11) is a CTA that is frequently expressed in esophageal cancer and is correlated...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgaa057
更新日期:2020-07-14 00:00:00
abstract::To determine the contribution of susceptibility loci in explaining the genetic basis of acute lymphoblastic leukemia (ALL), we genotyped 29 high-potential candidate genes with 672 tagged single-nucleotide polymorphisms (SNPs) in a sample (163 cases and 251 healthy controls) of Caucasian children. Fifty SNPs in 15 gene...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgr091
更新日期:2011-09-01 00:00:00
abstract::IARC Monographs are globally-recognized assessments of carcinogenicity published by International Agency for Research on Cancer and directed to national authorities as a contribution to public health. Currently, though not constraining the scope of studies examined, Monographs are declared to involve only hazard ident...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgaa129
更新日期:2020-12-01 00:00:00