Abstract：
:A radioimmunoassay (RIA) was developed to measure cortisone in Atlantic salmon plasma. Diatomaceous earth microcolumn chromatography was used to separate the cortisone from other steroids. The RIA method following chromatography was validated by analyzing each of six samples of blood by double isotope derivative assay (DIDA) and RIA. The RIA gave significantly lower plasma cortisone concentrations (P less than 0.05) than the DIDA: the correlation coefficient was 0.84. Accuracy of the RIA was also demonstrated by addition of radioinert cortisone and measurement of increasing volumes of plasma. Specificity of the antisera was very good. Sensitivity of the RIA was 11 pg/RIA tube or 0.025 micrograms cortisone/dl plasma corrected for recovery and fraction assayed. Plasma cortisone concentrations in adult Atlantic salmon ranged from 2 to 26 micrograms/dl. The mean plasma cortisone concentration of salmon in fresh water was not significantly different from that in sea water. The mean cortisone/cortisol ratio in both adult and juvenile Atlantic salmon was between 1.40 and 1.92 indicating that cortisone and not cortisol may be the principal corticosteroid in Atlantic salmon.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Weisbart M,McGowan LKdoi
10.1016/0016-6480(84)90014-5subject
Has Abstractpub_date
1984-09-01 00:00:00pages
429-36issue
3eissn
0016-6480issn
1095-6840journal_volume
55pub_type
杂志文章abstract：:The asymmetrical endostyle of Branchiostoma larvae contains two different zones of mucus-producing cells which metamorphose to the paired zones 2 and 4 respectively in the endostyle of the adult. In both the larva and the adult these zones are parts of the food-trapping mechanism. An endostyle zone, which has a positi...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(84)90028-5
更新日期：1984-11-01 00:00:00
abstract：:Stress is a known inhibitor of reproductive function. The mechanisms by which stress acts to influence the reproductive axis have been intensely studied and appear to be extremely varied. Gonadotropin-releasing hormone (GnRH) is a critical component of the vertebrate reproductive axis and directly causes pituitary gon...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.05.010
更新日期：2008-07-01 00:00:00
abstract：:Several lines of evidence support a role of endogenous opioids in the brain's regulation of gonadotropin secretion in birds and mammals, although the neuroanatomic basis of such regulation is not clear. We used double-label fluorescence immunohistochemistry employing polyclonal antisera raised in sheep against chicken...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(02)00029-1
更新日期：2002-06-15 00:00:00
abstract：:The role of the pituitary in regulating interrenal activity in coho salmon (Oncorhynchus kisutch) was examined using short- (1 week) or long- (10 week) term hypophysectomized (Hx) animals. In experiments in which Hx animals were injected with ovine growth hormone (oGH), triiodothyronine, or vehicle, plasma cortisol wa...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1993.1179
更新日期：1993-12-01 00:00:00
abstract：:Proper functioning of the endocrine stress axis requires communication between the stress axis and other regulatory mechanisms. We here describe an intimate interplay between the stress axis and recombinant human leptin (rhLeptin) in a teleostean fish, the common carp Cyprinus carpio. Restraint stress (by netting up t...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.04.004
更新日期：2012-08-01 00:00:00
abstract：:The binding characteristics of sex steroid binding protein (SBP) were investigated in vitellogenic and nonreproductive female rainbow trout (Oncorhynchus mykiss), black bream (Acanthopagrus butcheri), and greenback flounder (Rhombosolea tapirina). The binding capacity of rainbow trout and black bream SBP was significa...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2000.7554
更新日期：2000-12-01 00:00:00
abstract：:The regulated secretory pathway is a hallmark of endocrine and neuroendocrine cells. This process comprises different sequential steps, including ER-associated protein synthesis, ER-to-Golgi protein transport, Golgi-associated posttranslational modification, sorting and packing of secretory proteins into carrier granu...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2011.08.017
更新日期：2012-01-01 00:00:00
abstract：:To examine the production of steroids with potential oocyte maturation-inducing or pheromonal activity in the goldfish (Carassius auratus) we have incubated mature ovaries of this species with 17-[3H]hydroxyprogesterone. The metabolites in the unconjugated, glucuronide, and sulfate fractions were identified by chromat...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(92)90044-k
更新日期：1992-09-01 00:00:00
abstract：:Three distinct thyroid hormone receptor (TR) cDNAs (gTRalphaA, gTRalphaB and gTRbeta) were cloned and sequenced in orange-spotted grouper (Epinephelus coioides). The cDNA of gTRalphaA consisted of 1555-bp and encoded a putative protein of 416 amino acids, while the cDNA of gTRalphaB consisted of 1459-bp and encoded 39...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.08.015
更新日期：2008-11-01 00:00:00
abstract：:The neuroactive steroids which are synthesized in the brain and nervous system are known as "Neurosteroids". These steroids have crucial functions such as contributing to the myelination and organization of the brain connectivity. Under the stressful circumstances, the concentrations of neurosteroid products such as a...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2015.09.030
更新日期：2016-01-01 00:00:00
abstract：:Sexual selection acts on traits that increase reproductive success. Variation in reproductive success is often higher among males than females. Consequently, sexual selection has been studied extensively in males while its possible role in females has only recently attracted considerable attention. In some cooperative...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2013.03.026
更新日期：2013-06-15 00:00:00
abstract：:The possibility that the tonic secretion of luteinizing hormone (LH) and chicken luteinizing hormone-releasing hormone I (LHRH-I) is regulated by an inhibitory action of endogenous opioid peptides was investigated in cockerels using the opiate receptor antagonist, naloxone. Baseline concentrations of plasma LH in the ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(90)90308-9
更新日期：1990-02-01 00:00:00
abstract：:In bony fishes, both corticotropin-releasing factor (CRF) and urotensin-I play a role in the regulation of interrenal glucocorticoid release. The rainbow trout, Oncorhynchus mykiss, is a useful model for understanding the mechanisms of stress and the hypothalamo-pituitary-interrenal axis because of its phylogenetic po...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1999.7290
更新日期：1999-08-01 00:00:00
abstract：:Growth hormone (GH) and insulin-like growth factor-I (IGF-I) play major roles in the endocrine regulation of fish growth, but their interdependency and mode of action has not been well elucidated. The GH-IGF-I system is essential for normal vertebral growth in mouse, but this has not been studied in fish. To study the...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2004.12.005
更新日期：2005-05-15 00:00:00
abstract：:Full-length complementary deoxyribonucleic acid as well as genomic sequences encoding for two gastrointestinal appetite-related peptides, ghrelin and for gastrin-releasing peptide (GRP) were cloned from Atlantic cod (Gadus morhua) stomach using reverse transcription and rapid amplification of complementary deoxyribonu...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.12.004
更新日期：2009-02-01 00:00:00
abstract：:The adenohypophysial cell types in three species of sciaenid teleosts were identified using a combination of classical histochemical techniques and immunocytochemistry with antisera raised against piscine and human pituitary hormones. In general greater specificity for the different cell types was observed with antise...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90086-l
更新日期：1991-12-01 00:00:00
abstract：:In many fishes, dopamine (DA) strongly inhibits luteinizing hormone (LH) release by direct action at the pituitary level. In this study, the effect of DA on LH synthesis was examined by detecting its β-subunit mRNA level in immature grass carp pituitary cells. Results showed that DA inhibited LHβ mRNA expression and i...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.04.024
更新日期：2011-08-01 00:00:00
abstract：:Components of the renin angiotensin system have been identified in many nonmammalian vertebrates. However, in many of these animals, including reptiles, the physiological functions and importance of the system remain unclear. To aid in the study of the system in a reptile we modified a commercially available radioimmu...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90120-7
更新日期：1988-06-01 00:00:00
abstract：:Thyroid hormones (THs) are crucial for brain development and maturation in all vertebrates. Especially during pre- and perinatal development, disruption of TH signaling leads to a multitude of neurological deficits. Many animal models provided insight in the role of THs in brain development, but specific data on how t...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2017.04.001
更新日期：2017-06-01 00:00:00
abstract：:The in vivo actions of human and fish insulin-like growth factor (IGF)-I have been compared to extend the understanding of the metabolism of IGFs in fish and to identify potential differences in their actions. The effects of acute administration of these proteins on the incorporation of glucose into muscle glycogen an...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1999.7417
更新日期：2000-03-01 00:00:00
abstract：:We prepared monoclonal antibodies (mAbs) against a recombinant tethered follicle-stimulating hormone (rec-FSH) from Japanese eel Anguilla japonica that was produced in Escherichia coli. Positive hybridomas (clones eFA-C5, eFA-C10, eFA-C11, eFA-C12, eFA-C13, and eFB-C14) were selected by using the eel FSH antigen in EL...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2016.04.030
更新日期：2016-07-01 00:00:00
abstract：:Mammals generally respond to the important metabolic requirements imposed by thermoregulation and pregnancy by increasing plasma concentrations of glucocorticoid that promote the mobilization of body reserves and enhance energy use by tissues. This study examined the impact of distinct ambient temperatures and reprodu...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.02.007
更新日期：2011-05-01 00:00:00
abstract：:To examine the relative growth, endocrine, and gene expression effects of growth hormone (GH) transgenesis vs. GH protein treatment, wild-type non-transgenic and GH transgenic coho salmon were treated with a sustained-release formulation of recombinant bovine GH (bGH; Posilac). Fish size, specific growth rate (SGR), a...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.03.002
更新日期：2012-05-15 00:00:00
abstract：:PACAP (pituitary adenylate cyclase activating polypeptide) is widely distributed neuropeptide acting via three subtypes of receptors, PAC(1), VPAC(1) and VPAC(2). Here we examined the localisation and nature of PACAP-immunoreactive nerves in the rat thyroid and parathyroid glands and defined the distribution of PAC(1)...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.11.026
更新日期：2011-03-01 00:00:00
abstract：:The androgen concentration in birds' eggs varies with laying order, breeding conditions, and mate attractiveness. In passerine birds, mate attractiveness depends upon song quality. The aim of our study was to evaluate the effect of one criterion used by females to assess male song quality that is to say the presence o...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2004.05.010
更新日期：2004-09-01 00:00:00
abstract：:The pattern and kinetics of degradation of native salmon gonadotropin-releasing hormone (sGnRH) and mammalian leuteinizing hormone-releasing hormone (LHRH) by pituitary bound enzymes were studied in the gilthead seabream, Sparus aurata. sGnRH and LHRH were incubated for different periods of time with membrane or cytos...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(90)90115-3
更新日期：1990-08-01 00:00:00
abstract：:Birds use both the corticosterone stress response and immune system to meet physiological challenges during exposure to adverse climatic conditions. To assess the stress level and immune response of the Asian Great Bustard during conditions of severe winter weather, we measured fecal corticosterone (CORT) and Immunogl...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2018.02.014
更新日期：2018-05-15 00:00:00
abstract：:The possible involvement of calcium in the regulation of steroidogenesis in the goldfish was investigated using preovulatory ovarian follicles incubated in vitro. Incubation of follicles in media deficient in calcium impaired testosterone production in response to human chorionic gonadotropin (hCG) in both the presenc...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90011-t
更新日期：1991-02-01 00:00:00
abstract：:Sexual dimorphism in prenatal development is widespread among vertebrates, including birds. Its mechanism remains unclear, although it has been attributed to the effect of maternal steroid hormones. The aim of this study was to investigate how increased levels of steroid hormones in the eggs influence early embryonic ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2017.09.004
更新日期：2017-12-01 00:00:00
abstract：:Asp1,Val5-angiotensin II (AII) caused dose-dependent increases in aortic blood pressure (BP) when injected into anesthetized Pekin ducks. Norepinephrine (NE) and epinephrine (E) concentrations in arterial plasma were found to be elevated 1 min after injection of AII, when BP was peaking, while plasma levels of unconju...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(83)90065-5
更新日期：1983-09-01 00:00:00
