Abstract:
:To examine the relative growth, endocrine, and gene expression effects of growth hormone (GH) transgenesis vs. GH protein treatment, wild-type non-transgenic and GH transgenic coho salmon were treated with a sustained-release formulation of recombinant bovine GH (bGH; Posilac). Fish size, specific growth rate (SGR), and condition factor (CF) were monitored for 14 weeks, after which endocrine parameters were measured. Transgenic fish had much higher growth, SGR and CF than non-transgenic fish, and bGH injection significantly increased weight and SGR in non-transgenic but not transgenic fish. Plasma salmon GH concentrations decreased with bGH treatment in non-transgenic but not in transgenic fish where levels were similar to controls. Higher GH mRNA levels were detected in transgenic muscle and liver but no differences were observed in GH receptor (GHR) mRNA levels. In non-transgenic pituitary, GH and GHR mRNA levels per mg pituitary decreased with bGH dose to levels seen in transgenic salmon. Plasma IGF-I was elevated with bGH dose only in non-transgenic fish, while transgenic fish maintained an elevated level of IGF-I with or without bGH treatment. A similar trend was seen for liver IGF-I mRNA levels. Thus, bGH treatment increased fish growth and influenced feedback on endocrine parameters in non-transgenic but not in transgenic fish. A lack of further growth stimulation of GH transgenic fish suggests that these fish are experiencing maximal growth stimulation via GH pathways.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Raven PA,Sakhrani D,Beckman B,Neregård L,Sundström LF,Björnsson BT,Devlin RHdoi
10.1016/j.ygcen.2012.03.002subject
Has Abstractpub_date
2012-05-15 00:00:00pages
143-52issue
1eissn
0016-6480issn
1095-6840pii
S0016-6480(12)00084-6journal_volume
177pub_type
杂志文章abstract::Sexual selection acts on traits that increase reproductive success. Variation in reproductive success is often higher among males than females. Consequently, sexual selection has been studied extensively in males while its possible role in females has only recently attracted considerable attention. In some cooperative...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2013.03.026
更新日期:2013-06-15 00:00:00
abstract::The potential effects of growth hormone (GH), prolactin (Prl), and triiodothyronine (T3) on gill Na+,K+-ATPase activity and corticosteroid receptor (CR) concentration (Bmax) and dissociation constant (Kd) were examined in juvenile Atlantic salmon (Salmo salar). Compared to controls, fish injected with GH (ovine, 5.0 m...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1998.7172
更新日期:1998-11-01 00:00:00
abstract::Insulin and IGF-I binding has been studied in brown trout (Salmo trutta) wheat germ agglutinin semipurified receptors from embryos (organogenesis), larvae (yolk sac), juveniles (2.98 +/- 0.21 g bw) and adults (111.6 +/- 6.92 and 522 +/- 53 g bw). Embryos and larvae were sampled at 5 and 12 weeks after fertilization (D...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.2001.7621
更新日期:2001-05-01 00:00:00
abstract::It has long been held that cortisol, a glucocorticoid in many vertebrates, performs glucocorticoid and mineralocorticoid actions in the teleost fish since it lacks aldosterone. However, in addition to the counterparts of tetrapod mineralocorticoid receptors (MRs), 11-deoxycorticosterone (DOC) has been recently identif...
journal_title:General and comparative endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.ygcen.2012.11.016
更新日期:2013-01-15 00:00:00
abstract::Ultrastructural comparison of different types of perisympathetic organs (POs) in three species of Coleoptera (Chrysocarabus auronitens, Oryctes rhinoceros, and Tenebrio molitor) showed that the structure of these organs was not related to their morphological types but to their topography. Two kinds of PO structure may...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(83)90203-4
更新日期:1983-03-01 00:00:00
abstract::We have examined the effect of ambient water temperature on the ability of juvenile rainbow trout to respond to estradiol (E2) injection with vitellogenin (Vg) synthesis. Vg appeared in serum within 24 hr of E2 injection in fish kept at 15 degrees and rose to 70 mg/ml over a 10-day treatment period during which four i...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1993.1031
更新日期:1993-02-01 00:00:00
abstract::In Seriola species, exposure to a long photoperiod regime is known to induce ovarian development. This study examined photoperiodic effects on pituitary gene expression and plasma levels of follicle-stimulating hormone (Fsh) and luteinizing hormone (Lh) in previtellogenic greater amberjack (Seriola dumerili). The fish...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2018.09.007
更新日期:2018-12-01 00:00:00
abstract::Somatolactin (SL) and SL receptor (SLR) belong to the growth hormone and cytokine type I receptor superfamilies, respectively. However, further research is required to define the duplications and functions of SL and its receptors in basal vertebrates including environmental background color adaptation in fish. In the ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2011.12.023
更新日期:2012-03-01 00:00:00
abstract::Ovaprim (OVP) is used as an effective spawning inducer for artificial breeding of fishes and contains a salmon gonadotropin-releasing hormone analogue and a dopamine receptor-2 antagonist, domperidone. Previously, we have shown that vasotocin (VT) stimulates ovarian final oocyte maturation, hydration, and ovulation th...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2013.11.009
更新日期:2014-01-01 00:00:00
abstract::By use of the indirect immunofluorescence method in conjunction with an elution-restaining technique, cells in the caudal neurosecretory system of the big skate (Raja binoculata) and the plainfin midshipman (Porichthys notatus) were shown to contain both urotensin I (UI)/corticotropin-releasing factor (CRF) and uroten...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(86)90167-x
更新日期:1986-08-01 00:00:00
abstract::The importance of late winter pairing activity on the neuroendocrinology of wild waterfowl is unknown. In this study, we examined the sex-related differences in the roles of late winter pairing activity and seasonal influences on neuroendocrine and reproductive physiology in both male and female mallards. Our main goa...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.2000.7469
更新日期:2000-05-01 00:00:00
abstract::This study investigated the pathways to many monoamines and their metabolites in the central nervous system of the frog Rana nigromaculata and the toad Bufo bufo japonicus during embryonic development and metamorphosis. Metabolites were analyzed by three-dimensional HPLC. The two species provided evidence of similar p...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1997.6885
更新日期:1997-06-01 00:00:00
abstract::Methyl farnesoate (MF) stimulation of ecdysteroid secretion by Y-organs of the crab, Cancer magister, is demonstrated. Isolated Y-organs that were incubated with a mandibular organ (MO) secreted significantly higher levels of ecdysteroids into the medium when compared to those incubated in the absence of a MO. MOs sec...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1993.1050
更新日期:1993-03-01 00:00:00
abstract::In fish, luteinizing hormone (LH) stimulates processes leading to final oocyte maturation and ovulation in females, and spermiation in males. The hormone is a heterodimeric glycoprotein composed of two non-covalently associated subunits. In this study, we describe the expression of tilapia LH (tLH) as a biologically a...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2004.10.016
更新日期:2005-02-01 00:00:00
abstract::Normal saline, L-thyroxine (L-T4, 0.5 micrograms/bird/day), and testosterone (100 micrograms/bird/day) were administered separately to intact, thyroidectomized, and castrated spotted munia over a 30-day period and the effects on basal metabolic rate (BMR), gonads, and body weight were recorded. Thyroidectomy and castr...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(84)90062-5
更新日期:1984-10-01 00:00:00
abstract::Plasma glucagon levels in 11 different teleosts and in dogfish were evaluated using an adapted classical mammalian radioimmunoassay (RIA). The values obtained are considered relative; sensitivity was inferior to the original method, but the validation experiment results are acceptable. The mean value of the coefficien...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(86)90131-0
更新日期:1986-09-01 00:00:00
abstract::Previous reports have shown that adenosine in rat inhibits both spontaneous and ACTH-induced release of corticosteroids through activation of adenosine A1 receptors. In the present study, we have investigated the possible effect of adenosine in the secretion of corticosteroids in amphibians using a perfusion technique...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(92)90070-z
更新日期:1992-06-01 00:00:00
abstract::The brain of many teleost fish species, including the goldfish Carassius auratus, expresses exceptionally high levels of cytochrome P450 aromatase (estrogen synthetase). To begin investigating the molecular and cellular targets of estrogen action in goldfish brain, a polymerase chain reaction (PCR) cloning strategy wa...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1998.7217
更新日期:1999-03-01 00:00:00
abstract::Testosterone- and progesterone-like substances were detected by radioimmunoassay (RIA) in chromatographed extracts of hemolymph from larvae of Sarcophaga bullata (S.B.). Gas chromatographic (GC) analysis after heptafluorobutyric acid (HFBA) derivation of hemolymph extracts, purified by paper and silica gel column chro...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(83)90175-2
更新日期:1983-12-01 00:00:00
abstract::Follicle-stimulating hormone (FSH) stimulates aromatase activity of immature rat Sertoli cells cultured in vitro in the presence of an androgen substrate, a phenomenon that can be used to measure FSH bioactivity. This paper analyzes the effects of sera from various animal species on both aromatase activity and morphol...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1994.1106
更新日期:1994-07-01 00:00:00
abstract::Immunochemical investigations, using dot immunobinding assay (DIA) and enzyme-linked immunosorbent assay (ELISA), and immunocytochemical studies reveal the following new information about crustacean vitellogenesis-inhibiting hormone (VIH): (1) The structure of VIH is sufficiently different from that of the other sinus...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(87)90187-0
更新日期:1987-09-01 00:00:00
abstract::Corticosterone, at baseline and moderately elevated levels, is thought to regulate energy mobilization during the predictable life-history cycle. In birds, corticosterone is known to be moderately elevated during migration, and some experiments on captive, but migratory active birds have shown that exogenous corticost...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2013.02.042
更新日期:2013-06-01 00:00:00
abstract::The role of ovarian hormones in the expression of parental behavior and in the regulation of LH secretion was investigated in incubating commercial meat-type hens. After ovariectomy, incubating hens continued to incubate eggs normally and brooded day-old chicks given to replace eggs, in a manner similar to sham-ovarie...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1996.0013
更新日期:1996-01-01 00:00:00
abstract::We used a free-ranging, seasonally breeding adult male songbird, the rufous-winged sparrow, Aimophila carpalis, to investigate the effects of acute stress-induced by capture followed by restraint, on the hypothalamo-pituitary-testicular axis. Intra- and interindividual comparisons revealed that males decreased their p...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2010.07.009
更新日期:2010-10-01 00:00:00
abstract::Serum cortisol levels were measured in a total of 73 tammar wallabies maintained in a captive population at Macquarie University, NSW, Australia. Previous studies of corticosteroids in marsupials have generally involved low sample numbers, a diverse array of analytical techniques, and a variety of sampling conditions....
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/s0016-6480(03)00185-0
更新日期:2003-10-01 00:00:00
abstract::Relaxin-like gonad-stimulating peptide (RGP) of starfish Patiria (= Asterina) pectinifera is the first identified invertebrate gonadotropin to trigger final gamete maturation. Recently, chemical structures of RGP were identified in several species of starfish. Three kinds of RGP molecules are found in the class Astero...
journal_title:General and comparative endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.ygcen.2016.04.016
更新日期:2016-05-01 00:00:00
abstract::Recent studies have revealed nesfatin-1 as a hypothalamic neuropeptide, regulating food intake, energy expenditure and reproduction primarily by acting on the hypothalamic-pituitary-gonadal axis. Nesfatin-1 is also localized in several peripheral tissues including testes. However, functional significance of nesfatin-1...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2018.10.022
更新日期:2019-01-15 00:00:00
abstract::Fecal steroid analysis is an increasingly common non-invasive technique used in both captive and field studies to measure an animal's approximate hormonal levels and corresponding physiological state. Fecal collection in the field necessitates storage and transportation methods that will prevent the degradation of hor...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2006.06.010
更新日期:2007-01-01 00:00:00
abstract::Using frog ovarian follicles in vitro we assessed the role of the theca/epithelium (THEP) layer and granulosa cells in the production of steroids. The THEP layer and granulosa cell-enclosed oocytes (GCEOs) were separated from ovarian follicles of the frog, Rana nigromaculata, by microdissection. Intact follicles (IFs)...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1994.1077
更新日期:1994-05-01 00:00:00
abstract::By mapping the distribution of cholecystokinin (CCK) receptor types onto an established phylogenetic hypothesis of vertebrate relationships, we tested two hypothesis about the evolution of CCK receptors: (1) A single CCK receptor type, CCK-X, is the ancestral receptor, while CCK-A and CCK-B receptors represent derived...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1996.0047
更新日期:1996-04-01 00:00:00