Abstract：
:The brain of many teleost fish species, including the goldfish Carassius auratus, expresses exceptionally high levels of cytochrome P450 aromatase (estrogen synthetase). To begin investigating the molecular and cellular targets of estrogen action in goldfish brain, a polymerase chain reaction (PCR) cloning strategy was used to isolate an estrogen receptor (ER) complementary DNA (cDNA). The 2283-bp cDNA isolated from goldfish liver encoded a protein of 568 amino acids (aa) with an estimated molecular weight of 63,539. The goldfish ER had high overall sequence identity when compared to other vertebrate ER sequences: eel (64%), human beta subtype (59%), human alpha subtype (46%), medaka (46%), and rainbow trout (47%). The highest degree of conservation was seen in the DNA-binding (94-100%) and ligand-binding (67-79%) domains. Phylogenetic analysis of the ER gene family indicated that the goldfish and eel ER are most closely related to mammalian ERbeta subtypes, whereas previously identified fish, amphibian, and avian ER forms cluster separately with mammalian ERalpha subtypes. Using the goldfish ER cDNA (here designated gfERbeta), multiple mRNA species (3.1- 8.6 kb) were detected by Northern blot analysis in goldfish liver and ovary but expression was below detection in brain. Using reverse transcription-PCR analysis, gfERbeta mRNA was detected in forebrain, mid/hindbrain, pituitary, retina, liver, ovary, and testis. Further studies are required to determine whether an additional ERalpha subtype is present in the goldfish and whether ERalpha or ERbeta forms have evolutionary precedence in vertebrates.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Tchoudakova A,Pathak S,Callard GVdoi
10.1006/gcen.1998.7217keywords:
subject
Has Abstractpub_date
1999-03-01 00:00:00pages
388-400issue
3eissn
0016-6480issn
1095-6840pii
S0016-6480(98)97217-3journal_volume
113pub_type
杂志文章abstract：:In this study, the effect of acute and chronic insulin treatments on major factors involved in the control of muscle protein turnover were investigated in rainbow trout (Oncorhynchus mykiss). We found that acute but not chronic insulin administration leads to the induction of the phosphorylation of several key factors...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.03.026
更新日期：2011-07-01 00:00:00
abstract：:Twelve male edible dormice, captured in the autumn of 1979, were studied for 2 years under natural lighting in an animal enclosure with permanently open windows, at Montpellier, in Southern France (latitude 43 degrees 50' N). Ambient temperature fluctuated annually between +4 and 27 degrees, and the photoperiod betwee...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(83)90266-6
更新日期：1983-06-01 00:00:00
abstract：:Undifferentiated spermatogonia (Aund) or spermatogonial stem cells (SSCs) are committed to the establishment and maintenance of spermatogenesis and fertility throughout a male's life and are located in a highly specialized microenvironment called niche that regulates their fate. Although several studies have been deve...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2018.06.019
更新日期：2019-03-01 00:00:00
abstract：:Circannual and circadian variations in plasma levels of steroids were estimated by radioimmunoassay in the female and male catfish, Heteropneustes fossilis, over two consecutive annual reproductive cycles. In the female catfish, testosterone (T), estradiol-17 beta (E2), and estrone (E1) were detectable in the plasma o...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(83)90221-6
更新日期：1983-05-01 00:00:00
abstract：:Our previous study demonstrated that recombinant goldfish activin B stimulated goldfish FSHbeta but inhibited LHbeta expression. Similar to activin B, activin A also exhibited the inverse effects on the expression of the two gonadotropins. The novel dual effects of activins on FSH and LH in the goldfish raise an inter...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2003.08.012
更新日期：2004-01-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2003.11.004
更新日期：2004-02-01 00:00:00
abstract：:Several of the song control nuclei of songbirds, including HVc (higher vocal center) and Area X, contain melatonin receptor (MelR). In laboratory-housed male starlings, the densities of MelR in Area X change markedly according to reproductive state. MelR are down-regulated when starlings are photostimulated (in full b...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(03)00261-2
更新日期：2003-11-01 00:00:00
abstract：:Maintenance of internal ionic and acid-base homeostasis is critical for survival in all biological systems. Similar to mammals, aquatic fishes have developed sophisticated homeostatic mechanisms to mitigate metabolic or environmental disruptions in ionic and acid-base status of systemic body fluids via hormone-control...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2019.03.007
更新日期：2019-06-01 00:00:00
abstract：:Birds use both the corticosterone stress response and immune system to meet physiological challenges during exposure to adverse climatic conditions. To assess the stress level and immune response of the Asian Great Bustard during conditions of severe winter weather, we measured fecal corticosterone (CORT) and Immunogl...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2018.02.014
更新日期：2018-05-15 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90099-8
更新日期：1988-04-01 00:00:00
abstract：:In the hypothalamus, endocannabinoids affect neuroendocrine activity by means of Gonadotropin-Releasing-Hormone-I (GnRH-I) inhibition. Since most vertebrates, human included, possess at least two GnRH molecular forms, the aim of this work was to investigate the effect of endocannabinoids on GnRH molecular forms other ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.07.001
更新日期：2011-09-15 00:00:00
abstract：:The majority of birds use environmental cues to time their reproduction. Photoperiod is the most dominant cue, but other environmental factors may play a critical role in successful reproduction. Some previous studies show the effect of temperature on the timing of nest building and reproduction. Here we tested if the...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2020.113654
更新日期：2021-01-15 00:00:00
abstract：:The response of plasma prolactin (PRL) to manipulations of plasma calcium were investigated in coho salmon. Injection of 3.16 mumol EGTA/10 g body weight (to reduce the plasma calcium activity) resulted in significantly higher plasma PRL levels. Lower doses of EGTA (1.06 and 2.13 mumol/10 g body wt) had no effect on p...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(89)90197-4
更新日期：1989-03-01 00:00:00
abstract：:Migratory birds undergo metabolic remodeling in tissues, including increased lipid storage in white adipose and fatty acid uptake and oxidation in skeletal muscle, to optimize energy substrate availability and utilization in preparation for long-distance flight. Different tissues undergo gene expression changes in kee...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2018.08.009
更新日期：2018-11-01 00:00:00
abstract：:This study compares follicular function and ovulatory efficiency in 20 sows with obesity/leptin resistance genotype (Iberian pig) and 20 females of lean commercial crosses (Large White × Landrace; LW×L). Estrous cycle was synchronized with progestagens; ovulation was induced with eCG and hCG, in half of the females of...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.10.006
更新日期：2011-01-01 00:00:00
abstract：:The endocannabinoid system is a major regulator of food intake in many animal species. Studies conducted so far have mostly focused on mammals, and, therefore, in this study, the role of the endocannabinoid system in food intake in the sea bream Sparus aurata was investigated. The effect of different doses of the endo...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2009.09.017
更新日期：2010-04-01 00:00:00
abstract：:A detailed study was made of sex differentiation in three anteroposteriorly distributed body regions of the long gonadal fold in young Pacific hagfish (Eptatretus stouti) from 12.4 to 45 cm in length. In general, the posterior region, adjacent to the cloaca, remained undifferentiated, and only in specimens more than 2...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(90)90315-d
更新日期：1990-02-01 00:00:00
abstract：:To understand the effect of testosterone on sex differentiation, the quantities of testosterone (T) and estradiol-17beta (E2) in developing eggs of medaka (Oryzias latipes) were measured by radioimmunoassay, and the influence on sex differentiation of treating embryos with exogenous androgens was also examined. Endoge...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2005.07.003
更新日期：2006-01-01 00:00:00
abstract：:The crab-eating frog Fejervarya cancrivora inhabits mangrove swamps and marshes in Southeast Asia. In the present study, circulating angiotensin II (Ang II), aldosterone (Aldo), arginine vasotocin (AVT), and corticosterone (Cort) concentrations as well as various blood parameters were studied under osmotically stressf...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2013.10.013
更新日期：2014-01-01 00:00:00
abstract：:To study the effects of glucocorticoids and IGF-I on the modulation of growth in the tilapia Oreochromis mossambicus, we employed an epiceratobranchial cartilage radioisotope incorporation assay, wherein radiolabeled sulfate and thymidine uptakes are measured in vitro to indicate proteoglycan synthesis and cell prolif...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2000.7599
更新日期：2001-03-01 00:00:00
abstract：:As an alternative to radioimmunoassays, a simple, highly sensitive and quick enzymeimmunoassay (EIA) for determination of testosterone in blood plasma of yaks on microtitreplates using second antibody coating technique and testosterone-horseradish peroxidase as a label has been developed for the first time. The wells ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.07.007
更新日期：2008-09-15 00:00:00
abstract：:In this study, we cloned a full-length cDNA encoding vitellogenin (Vg) in the Fujian oyster Crassostrea angulata. The complete Vg cDNA consists of 5160 nucleotides with a long open reading frame encoding 1641 amino acid residues. The deduced amino acid sequence shared high similarity with the Vgs of other mollusc, fis...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2014.03.034
更新日期：2014-06-01 00:00:00
abstract：:Our previous research on range-expanding house sparrows in Kenya revealed that (i) range-edge birds released more corticosterone (CORT) in response to a stressor than range-core birds, ii) that range-edge birds were more exploratory than range-core birds, and that (iii) all birds exhibited extensive variation in genom...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2018.09.014
更新日期：2018-12-01 00:00:00
abstract：:Endocrine cells were detected at premetamorphosis, prometamorphosis, climax, and juvenile stages using an amine-inducing fluorescence technique with or without previous L-3,4-dihydroxyphenylalanine (L-DOPA) treatment. At premetamorphosis, serotonin cells exhibited yellow fluorescence in the gut primary epithelium of t...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90029-9
更新日期：1988-03-01 00:00:00
abstract：:Acid extracts of neurointermediate lobe (NIL) and pars distalis (PD) of the pituitary gland of the rainbow trout were radioimmunoassayed for alpha-melanocyte-stimulating hormone (alpha-MSH), beta-melanocyte-stimulating hormone (beta-MSH), adrenocorticotrophin (ACTH), and endorphin. The majority of the alpha-MSH, beta-...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(83)90062-x
更新日期：1983-09-01 00:00:00
abstract：:Thyroid-dependent stomach development occurs between approximately 35 and 50 days post-hatch (dph) in laboratory-reared summer flounder larvae. The process can be blocked by thiourea (TU), and TU effects are reversed by exogenous thyroxine (T4). To establish whether a window of sensitivity exists for T4-dependent gast...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/s0016-6480(03)00262-4
更新日期：2003-12-01 00:00:00
abstract：:Classical biotin deficiency in young chickens is characterized by a reduction in the activity of liver biotin-dependent enzymes which thus impairs gluconeogenesis during periods of food withdrawal. Because the normal bird maintains an elevated fasting blood glucose level, the ability of the classically biotin-deficien...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90232-2
更新日期：1987-01-01 00:00:00
abstract：:Asp1,Val5-angiotensin II (AII) caused dose-dependent increases in aortic blood pressure (BP) when injected into anesthetized Pekin ducks. Norepinephrine (NE) and epinephrine (E) concentrations in arterial plasma were found to be elevated 1 min after injection of AII, when BP was peaking, while plasma levels of unconju...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(83)90065-5
更新日期：1983-09-01 00:00:00
abstract：:In vivo studies were carried out in adult chickens in an attempt to evaluate the effectiveness of somatostatin (SRIF) in regulating hormone secretion from the splenic pancreatic lobe after 99% of the pancreatic mass was surgically ablated. Sixteen days after either sham operation or 99% pancreatectomy, birds were infu...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(86)90166-8
更新日期：1986-08-01 00:00:00
abstract：:Chronic confinement for 1 month caused a significant elevation of plasma cortisol but suppressed the levels of plasma testosterone and 11-ketotestosterone in sexually mature male brown trout. An acute handling stress for 1 hr elevated blood cortisol and ACTH levels and also suppressed circulating androgens. This andro...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/0016-6480(87)90036-0
更新日期：1987-11-01 00:00:00
