Abstract:
:The endocannabinoid system is a major regulator of food intake in many animal species. Studies conducted so far have mostly focused on mammals, and, therefore, in this study, the role of the endocannabinoid system in food intake in the sea bream Sparus aurata was investigated. The effect of different doses of the endocannabinoid anandamide (AEA), administered via water, was evaluated after different exposure times (30, 60 and 120 min) at both physiological and molecular levels. The results obtained indicate that fish exposed to AEA via water present approximately 1000-fold higher levels of AEA in both the brain and liver, which correlated with a significant increase in food intake and with the elevation of cannabinoid receptor 1 (CB(1)) and neuropeptide Y (NPY) mRNA levels in the brain. A peripheral effect of AEA was also observed, since a time-dependent increase in hepatic CB(1) mRNA and protein levels was detected. These effects were attenuated by the administration, again via water, of a selective cannabinoid CB(1) receptor antagonist (AM251). These findings indicate that the endocannabinoid AEA, at doses that stimulate food intake in fish, concomitantly stimulates the expression of the orexigenic peptide NPY as well that of its own receptor, thereby potentially enhancing its effect on food consumption. In agreement with a role of AEA in food intake in S. aurata, we found increased brain levels of both this and the other endocannabinoid, 2-arachidonoylglycerol (2-AG), following food deprivation.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Piccinetti CC,Migliarini B,Petrosino S,Di Marzo V,Carnevali Odoi
10.1016/j.ygcen.2009.09.017subject
Has Abstractpub_date
2010-04-01 00:00:00pages
259-67issue
2eissn
0016-6480issn
1095-6840pii
S0016-6480(09)00351-7journal_volume
166pub_type
杂志文章abstract::In mammals ghrelin has a diverse range of effects including stimulation of gut motility but although present in teleost fish its effects on motility have not been investigated. The present study used bioinformatics to search for fish paralogues of the ghrelin receptor and the closely related motilin receptor, and inve...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2007.05.016
更新日期:2008-01-01 00:00:00
abstract::Serum prolactin (PRL) and luteinizing hormone (LH) levels from mature female turkeys were determined following ovariectomy and daily subcutaneous injections for 4 or 9 days of 0.002-2.0 mg/kg estradiol benzoate (EB), 0.05-2.0 mg/kg progesterone (P), or their combinations. Serum PRL levels were increased (P less than 0...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(83)90159-4
更新日期:1983-10-01 00:00:00
abstract::The four-spine sculpin Cottus kazika is a euryhaline teleost, in which faster growth in seawater (SW) and freshwater (FW) has been reported. In this study, cDNA clones encoding growth hormone (GH) and insulin-like growth factor I (IGF-I) were isolated from this species to examine the involvement of the GH/IGF-I axis i...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/s0016-6480(02)00650-0
更新日期:2003-03-01 00:00:00
abstract::This study characterizes the seasonal reproductive cycle of female round stingrays (Urobatis halleri) in an open coastal site at Seal Beach, CA and a protected estuary at the Seal Beach National Wildlife Refuge (SBNWR). Female round stingrays were sampled from August 2004 to July 2006, and assessed for reproductive pa...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2009.12.009
更新日期:2010-04-01 00:00:00
abstract::Estrogen signalling is critical for ovarian differentiation in reptiles with temperature-dependent sex determination (TSD). To elucidate the involvement of estrogen in this process, adrenal-kidney-gonadal (AKG) expression of estrogen receptor (ERα) was studied at female-producing temperature (FPT) in the developing em...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2014.08.003
更新日期:2015-09-01 00:00:00
abstract::Secretin (Sct), traditionally a gastrointestinal hormone backed by a century long research, is now beginning to be recognized also as a neuroactive peptide. Substantiation by recent evidence on the functional role of Sct in various regions of the brain, especially on its potential neurosecretion from the posterior pit...
journal_title:General and comparative endocrinology
pub_type: 杂志文章,评审
doi:10.1016/j.ygcen.2012.11.017
更新日期:2013-01-15 00:00:00
abstract::TSH-like producing cells have been studied using the peroxidase-antiperoxidase (PAP) immunohistochemical technique and rabbit anti-human thyrotropin (TSH) antiserum in the pars distalis of five species of amphibian anuran tadpoles at different stages of development as well as in 1-year-old postmetamorphic animals. Thi...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(88)90302-4
更新日期:1988-07-01 00:00:00
abstract::Insulin (INS) plays a critical role in the growth, development, and metabolism of vertebrates. In this study, a cDNA encoding a novel insulin receptor (IR) subtype was isolated, cloned, and sequenced from the liver of rainbow trout. A 1525-bp cDNA encoding a partial amino acid sequence of the β-subunit including the t...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2010.09.005
更新日期:2010-12-01 00:00:00
abstract::In order to examine the role of thyroid hormones during salmonid development, techniques were developed for quantitative extraction of thyroxine from eggs, whole embryos, and alevins of chum salmon (Oncorhynchus keta) at various stages of development. Frozen eggs, embryos, alevins, or fry were homogenized in ice-cold ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(87)90068-2
更新日期:1987-10-01 00:00:00
abstract::Changes in the circulating levels of thyroid hormones measured by radioimmunoassay in yearling coho salmon in response to injection of bovine thyroid-stimulating hormone (TSH) were evaluated from January through August. Doses of TSH ranging from 0.2 to 6.4 micrograms/g body wt were ineffective in stimulating T4 in Jan...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(87)90087-6
更新日期:1987-12-01 00:00:00
abstract::In recent years, invertebrate peptides have been identified which share substantial homologies with vertebrate insulin and insulin-like growth factors (IGFs), indicating a high degree of conservation of insulin/IGF systems through animal evolution. In a previous study, we provided evidence for the presence of IGF-I-li...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1996.6824
更新日期:1997-03-01 00:00:00
abstract::Isolated brain-retrocerebral neurohemal complex of the silkworm of Bombyx mori was stimulated electrically and the released bombyxin (an insulin-like neuropeptide) was measured using time-resolved fluoroimmunoassay. The amount of bombyxin release depended on the number of stimulus pulses delivered to the axonal tract ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2004.03.009
更新日期:2004-07-01 00:00:00
abstract::Day- and nighttime contents of pineal melatonin and other indoleamines were measured in the Chinese hamster, Cricetulus griseus. The hamsters were inbred in our colony and kept in LD 14:10. Absolute levels of pineal melatonin and N-acetylserotonin contents were extremely low, but showed a significant circadian rhythm ...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1997.7014
更新日期:1998-01-01 00:00:00
abstract::Salmonids have four subtypes of insulin-like growth factor binding protein (IGFBP)-1, termed -1a1, -1a2, -1b1 and 1b2, owing to teleost- and a lineage-specific whole-genome duplications. We have previously produced recombinant proteins of masu salmon IGFBP-1a1 and -1b2 and conducted functional analysis. To further cha...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2020.113606
更新日期:2020-12-01 00:00:00
abstract::Carcinus maenas is widely recognized as one of the world's most successful marine invasive species; its success as an invader is due largely to its ability to thrive under varied environmental conditions. The physiological/behavioral control systems that allow C. maenas to adapt to new environments are undoubtedly und...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2016.05.013
更新日期:2016-09-01 00:00:00
abstract::The secretory response of the nasal salt gland of the north African agamid lizard Uromastix acanthinurus to injections of 1 M KCl and NaCl is correlated with changes in circulating levels of the corticosteroids aldosterone and corticosterone. KCl loading led to a progressive rise in plasma aldosterone levels, and NaCl...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(84)90186-2
更新日期:1984-05-01 00:00:00
abstract::Using frog ovarian follicles in vitro we assessed the role of the theca/epithelium (THEP) layer and granulosa cells in the production of steroids. The THEP layer and granulosa cell-enclosed oocytes (GCEOs) were separated from ovarian follicles of the frog, Rana nigromaculata, by microdissection. Intact follicles (IFs)...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1994.1077
更新日期:1994-05-01 00:00:00
abstract::Effects of adrenal corticosteroids on reproductive and endocrine functions of the gonads are well known, but reciprocal effects of gonadal hormones on the hypothalamo-pituitary-adrenal (HPA) axis and on adrenocortical steroidogenesis in particular have received much less attention. We investigated effects of gonadecto...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2008.07.018
更新日期:2008-09-01 00:00:00
abstract::The aim of our study was to confirm the role of tidal pattern on the coordination of oocyte maturation and spawning in common snook Centropomus undecimalis. To do so, we studied oocyte maturation during the spawning season in relation to the tidal pattern in both males and females by means of histology and hormonal pr...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2015.08.007
更新日期:2015-12-01 00:00:00
abstract::Reverse-phase HPLC employing five different solvent systems was used to determine the 125I-labeled products formed in rainbow trout by in vitro incubation at 12 degrees of the hepatic microsome fraction with L-thyroxine (T4) labeled with 125I in the outer phenyl ring. Trout were starved for 2 weeks or fed a 2% ration....
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(92)90081-t
更新日期:1992-03-01 00:00:00
abstract::In the present study we sought to investigate interactions between hypothalamic nitric oxide (NO) and ghrelin signaling on food intake and energy substrate utilization as measured by the respiratory exchange ratio (RER). Guide cannulae were unilaterally implanted in either the arcuate (ArcN) or paraventricular (PVN) n...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2017.05.017
更新日期:2017-09-01 00:00:00
abstract::Fluctuations in plasma androgen (testosterone and 5 alpha-dihydrotestosterone), corticosterone (B), and luteinizing hormone (LH) of male bullfrogs in central California were measured during the spring mating season. Androgen and LH levels generally increased in the population prior to the initiation of chorusing and e...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(85)90343-0
更新日期:1985-05-01 00:00:00
abstract::Chickens were used as a model to further analyze the efficacy and specificity of L-692,429, a novel nonpeptidyl mimic of growth hormone (GH)-releasing peptide-6 (GHRP-6), which is a specific GH-releasing secretagogue in mammals. Actions at the level of the pituitary and the hypothalamus were studied. Pituitaries isola...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.1998.7102
更新日期:1998-08-01 00:00:00
abstract::Leach's storm-petrels accumulate large amounts of body mass throughout the nestling period. Approximately 4days before fledging, nestlings weigh 50-100% more than adults. In order to shed this excess mass, nestlings engage in behavioral anorexia, and leave the burrow when they are light enough to fly. During this pref...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2010.07.019
更新日期:2010-10-01 00:00:00
abstract::The aim of this work was to study, in the Cebus apella monkey, the developmental changes in the microanatomy of the utero-ovarian ligament (UOL) and whether their vascular and neural elements might be involved in the transfer of signals between the ovaries and uterus. Sections including uterus, UOL, and ovary obtained...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2005.06.006
更新日期:2005-12-01 00:00:00
abstract::The effects of epinephrine (E), norepinephrine (NE), phenylephrine (PHE), and isoproterenol (ISO) were studied in vivo on the blood pressure and heart rate of the conscious American bullfrog, Rana catesbeiana. Bullfrogs were chronically cannulated with a T cannula in the right sciatic artery. In warm-acclimated (22 de...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/0016-6480(85)90401-0
更新日期:1985-09-01 00:00:00
abstract::The G-protein-coupled receptor 54 (muGPR54) cDNA was cloned from the brain of the grey mullet, and its expression level, as well as those of the gonadotropin-releasing hormones (GnRH1, GnRH2, GnRH3) and dopamine receptor D2 (drd2), in the brain, pituitary and ovary of pubertal fish (early, intermediate, advanced) were...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2006.09.008
更新日期:2007-01-15 00:00:00
abstract::The courtship behavior of the male ring dove (Streptopelia risoria) combines aggressive displays (chasing, bowing) and nest-oriented displays (nest soliciting). Aggressive displays depend on circulating testosterone, whereas nest soliciting is estrogen-dependent and appears to depend on the aromatization of androgen i...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1006/gcen.2001.7608
更新日期:2001-04-01 00:00:00
abstract::Thyroid hormones (TH) are key regulators of metabolism and development, yet our understanding of the variability in serum TH concentrations in free-ranging marine mammals is limited. Thus, we examined the interrelationships between TH and age, sex, reproductive status, geographic location, and ocean temperatures in wi...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2011.08.021
更新日期:2011-11-01 00:00:00
abstract::The neuropeptide gonadotropin-releasing hormone (GnRH) plays an important role in the control of reproductive functions. Vertebrates possess multiple GnRH isoforms that are classified into three main groups, namely GnRH1, GnRH2 and GnRH3. In the present study, we show that the chromosomal organization of the three GnR...
journal_title:General and comparative endocrinology
pub_type: 杂志文章
doi:10.1016/j.ygcen.2010.11.017
更新日期:2011-02-01 00:00:00