当前位置： SCI文献检索 > GENERAL AND COMPARATIVE ENDOCRINOLOGY期刊下所有文献 > Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica.

Production and characterization of monoclonal antibodies against recombinant tethered follicle-stimulating hormone from Japanese eel Anguilla japonica.

Abstract：

:We prepared monoclonal antibodies (mAbs) against a recombinant tethered follicle-stimulating hormone (rec-FSH) from Japanese eel Anguilla japonica that was produced in Escherichia coli. Positive hybridomas (clones eFA-C5, eFA-C10, eFA-C11, eFA-C12, eFA-C13, and eFB-C14) were selected by using the eel FSH antigen in ELISA, and anti-eel FSH mAbs were purified from culture supernatants by performing affinity chromatography. Three of the 6mAbs were characterized and their isotypes were identified as IgG2b (eFA-C5 and eFA-C11) and IgG1 (eFB-C14). In western blotting assays, the mAbs recognized the antigen as a 24.3-kDa band, and further detected bands of 34 and 32kDa in the supernatants of CHO cells transfected with cDNA encoding tethered eel FSHβ/α and LHβ/α, respectively. PNase F-mediated deglycosylation of the recombinant proteins resulted in a drastic reduction in their molecular weight, to 7-9kDa. The mAbs eFA-C5 and eFA-C11 recognized the eel FSHα-subunit that is commonly encoded among glycoprotein hormones, whereas eFB-C14 recognized the eel FSHβ-subunit, and immunohistochemical analysis revealed that the staining by these mAbs was specifically localized in the eel pituitary. We also established an ELISA system for detecting rec-tethered FSHβ/α and LHβ/α produced from CHO cell lines. Measurement of biological activities in vitro revealed that only weak activity of rec-FSHβ/α was detected. The activity of rec-LHβ/α was found to be increased in a dose-dependent manner for eel oocyte maturation.

journal_name

Gen Comp Endocrinol

journal_title

General and comparative endocrinology

authors

Kim DJ,Park CW,Kim DW,Park HK,Byambaragchaa M,Lee NS,Hong SM,Seo MY,Kang MH,Min KS

doi

10.1016/j.ygcen.2016.04.030

subject

Has Abstract

pub_date

2016-07-01 00:00:00

pages

8-15

eissn

0016-6480

issn

1095-6840

pii

S0016-6480(16)30113-7

journal_volume

233

pub_type

杂志文章

相关文献

GENERAL AND COMPARATIVE ENDOCRINOLOGY文献大全
  • Changes in plasma and gonadal steroid hormones in relation to the reproductive cycle and the sex inversion process in the protandrous seabass, Lates calcarifer.

    abstract：:Plasma and gonadal levels of several gonadal steroids (testosterone, 11-ketotestosterone, androstenedione, 11 beta-hydroxyandrostenedione, 17 beta-estradiol, and estrone) were measured by RIA in the protandrous seabass, Lates calcarifer, throughout an annual reproductive cycle. Twenty to 25 fish were killed every mont...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1993.1170

    authors： Guiguen Y,Jalabert B,Thouard E,Fostier A

    更新日期：1993-12-01 00:00:00

  • Growth hormone stimulates hepatic thyroxine 5'-monodeiodinase activity and 3,5,3'-triiodothyronine levels in rainbow trout (Salmo gairdneri).

    abstract：:Intraperitoneal injection of rainbow trout (Salmo gairdneri) with 0.4 microgram/g of human growth hormone (hGH) increased plasma levels of 3,5,3'-triiodo-L-thyronine (T3) and Vmax of the hepatic microsomal 5'-monodeiodinase enzyme (5'D) that converts thyroxine (T4) to T3, with no effect on Km or plasma T4 levels. A do...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(90)90002-4

    authors： MacLatchy DL,Eales JG

    更新日期：1990-05-01 00:00:00

  • Plasma glucagon levels in different species of fish.

    abstract：:Plasma glucagon levels in 11 different teleosts and in dogfish were evaluated using an adapted classical mammalian radioimmunoassay (RIA). The values obtained are considered relative; sensitivity was inferior to the original method, but the validation experiment results are acceptable. The mean value of the coefficien...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(86)90131-0

    authors： Gutierrez J,Fernandez J,Blasco J,Gesse JM,Planas J

    更新日期：1986-09-01 00:00:00

  • Hepatic insulin-like growth-factor binding protein (igfbp) responses to food restriction in Atlantic salmon smolts.

    abstract：:The growth hormone (Gh)/insulin-like growth-factor (Igf) system plays a central role in the regulation of growth in fishes. However, the roles of Igf binding proteins (Igfbps) in coordinating responses to food availability are unresolved, especially in anadromous fishes preparing for seaward migration. We assayed plas...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2016.05.015

    authors： Breves JP,Phipps-Costin SK,Fujimoto CK,Einarsdottir IE,Regish AM,Björnsson BT,McCormick SD

    更新日期：2016-07-01 00:00:00

  • Targeted expression of androgen receptors in tooth-forming tissues of a neotropical salamander (Bolitoglossa schizodactyla) enables region-specific formation of dimorphic types of teeth in the male.

    abstract：:Bicuspid teeth are a morphological characteristic in adult metamorphosed urodeles. However, during the mating season the males of many species of plethodontid salamanders (Urodela, Plethodontidae) from northern America bear large monocuspid teeth on their jaws. Since androgen levels are elevated during the mating seas...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0016-6480(03)00216-8

    authors： Ehmcke J,Wistuba J,Clemen G,Schlatt S

    更新日期：2003-10-15 00:00:00

  • Identification and expression of nuclear receptor genes and ecdysteroid titers during nymphal development in the spider Agelena silvatica.

    abstract：:Ecdysteroids play an essential role in the regulation of the molting processes of arthropods. Nuclear receptors of the spider Agelena silvatica that showed high homology with other arthropods especially in the functional domains were identified, two isoforms of ecdysone receptor (AsEcRA, AsEcRB), retinoid X receptor (...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2017.01.032

    authors： Honda Y,Ishiguro W,Ogihara MH,Kataoka H,Taylor D

    更新日期：2017-06-01 00:00:00

  • Parathyroid hormone-related protein in teleost fish.

    abstract：:A brief description is given of the discovery of PTHrP and the roles of the peptide in mammalian physiology. Next, the occurrence of PTHrP in the earliest vertebrates, sharks, skates and fishes, is reviewed and the calciotropic functions of PTHrP are addressed more specifically in fishes. Parathyroid hormone-related p...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ygcen.2006.11.010

    authors： Abbink W,Flik G

    更新日期：2007-06-01 00:00:00

  • Thyroidectomy prevents the development of photorefractoriness and the associated rise in plasma prolactin in starlings.

    abstract：:Intact and radiothyroidectomized male starlings were transferred from short (8-hr) to long (16-hr) day lengths and measurements made of testicular development and of plasma prolactin concentrations. In intact birds the testes reached full maturity within 3 weeks and regressed after 6 weeks of photostimulation, indicat...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(84)90179-5

    authors： Goldsmith AR,Nicholls TJ

    更新日期：1984-05-01 00:00:00

  • Effects of five natural gonadotropin-releasing hormones on cell suspensions of marine bivalve gonad: stimulation of gonial DNA synthesis.

    abstract：:Gonadotropin-releasing hormones (GnRH) constitute a family of neuropeptides which are important regulators of reproduction in vertebrates. The effect of mammalian GnRH (mGnRH), salmon GnRH, chicken GnRH-I, chicken GnRH-II, and lamprey GnRH-I on [3H]thymidine incorporation into DNA of dissociated gonadal cells of marin...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1998.7186

    authors： Pazos AJ,Mathieu M

    更新日期：1999-01-01 00:00:00

  • Estrogen responsiveness of vitellogenin gene expression in rainbow trout (Oncorhynchus mykiss) kept at different temperatures.

    abstract：:We have examined the effect of ambient water temperature on the ability of juvenile rainbow trout to respond to estradiol (E2) injection with vitellogenin (Vg) synthesis. Vg appeared in serum within 24 hr of E2 injection in fish kept at 15 degrees and rose to 70 mg/ml over a 10-day treatment period during which four i...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1993.1031

    authors： Mackay ME,Lazier CB

    更新日期：1993-02-01 00:00:00

  • Comparison of recombinant barramundi and human insulin-like growth factor (IGF)-I in juvenile barramundi (Lates calcarifer): in vivo metabolic effects, association with circulating IGF-binding proteins, and tissue localisation.

    abstract：:The in vivo actions of human and fish insulin-like growth factor (IGF)-I have been compared to extend the understanding of the metabolism of IGFs in fish and to identify potential differences in their actions. The effects of acute administration of these proteins on the incorporation of glucose into muscle glycogen an...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1999.7417

    authors： Degger B,Upton Z,Soole K,Collet C,Richardson N

    更新日期：2000-03-01 00:00:00

  • Number of mast cells in the Harderian gland of the lizard Podarcis sicula sicula (Raf): the annual cycle and its relation to environmental factors and estradiol administration.

    abstract：:The Harderian gland of the lizard Podarcis sicula sicula (Raf) contains connective tissue type mast cells whose numbers vary during the year showing two peaks, one in spring the other in winter. No sex differences are found throughout the year. Thermal and photoperiodic manipulations indicate that only temperature inf...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1997.6925

    authors： Vitiello II,Baccari GC,Di Matteo L,Rusciani A,Chieffi P,Minucci S

    更新日期：1997-09-01 00:00:00

  • Species specificity in the magnitude and duration of the acute stress response in Mediterranean marine fish in culture.

    abstract：:The aim of the present study was to examine the species-specific stress response for seven Mediterranean fishes in culture. Also, to evaluate the method of measuring free cortisol concentration in the rearing water as a non-invasive and reliable indicator of stress in marine species, of aquaculture importance. Gilthea...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2011.06.004

    authors： Fanouraki E,Mylonas CC,Papandroulakis N,Pavlidis M

    更新日期：2011-09-01 00:00:00

  • How functional genomics and genetics complements insect endocrinology.

    abstract：:Insects are the most abundant animal group on Earth and have been the subject of genetic and physiological studies since the beginning of the 19th century. The public interest in understanding their biology increased as many insects have proven to exert a severe impact on human welfare and the environment. To trigger ...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ygcen.2007.06.021

    authors： Reumer A,Van Loy T,Clynen E,Schoofs L

    更新日期：2008-01-01 00:00:00

  • Characterization of L-thyroxine transport into hepatocytes isolated from juvenile rainbow trout (Oncorhynchus mykiss).

    abstract：:Uptake of L-thyroxine (T4) by isolated trout hepatocytes was characterized after 1-min incubation of cells in a Hanks' balanced salts medium containing glucose and [125I]T4. Centrifugation through silicone oil and glycine buffer (pH 10.5) was used to separate cells from the medium and minimize extracellular binding of...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1993.1057

    authors： Riley WW Jr,Eales JG

    更新日期：1993-04-01 00:00:00

  • The effects of age, sex, season and geographic region on circulating serum cortisol concentrations in threatened and endangered Steller sea lions (Eumetopias jubatus).

    abstract：:The role of stress in the decline of Steller sea lions has become of interest in the identification of factors leading to their drastic decline and subsequent failure to recover. Serum cortisol was validated as a potential indicator of stress in three sub-populations inhabiting the majority of the Steller sea lions ra...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2009.06.006

    authors： Myers MJ,Litz B,Atkinson S

    更新日期：2010-01-01 00:00:00

  • Developmental expression and subcellular localization of wallaby gonadotropin-releasing hormone receptor and its splice variants.

    abstract：:The developmental expression of gonadotropin-releasing hormone receptor (GnRH-R) and its splice variants was examined in the gonads of tammar wallaby pouch young in order to elucidate the functional role of GnRH-R in the developing testis and ovary. Wallaby GnRH-R, like eutherian GnRH-Rs, contains three exons and two ...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0016-6480(03)00146-1

    authors： Cheung TC,Hearn JP

    更新日期：2003-08-01 00:00:00

  • Relationships among hormonal changes, cuticular hydrocarbons, and attractiveness during the first gonadotropic cycle of the female Calliphora vomitoria (Diptera).

    abstract：:Attractiveness in adult females of Calliphora vomitoria is correlated with ovarian development and there is a marked increase during the previtellogenic and vitellogenic periods. The development of attractiveness may result from the combined actions of ecdysteroids and juvenile hormone. A rise in total hydrocarbons pa...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(90)90166-j

    authors： Trabalon M,Campan M,Porcheron P,Clement JL,Baehr JC,Moriniere M,Joulie C

    更新日期：1990-11-01 00:00:00

  • On the role of urocortin 1 in the non-preganglionic Edinger-Westphal nucleus in stress adaptation.

    abstract：:The discovery of novel members of the CRF neuropeptide family, urocortin 1 (Ucn1), urocortin 2 and 3 has provided important insights into stress adaptation pathways, and predicted that stress adaptation involves more systems than the HPA-axis alone. This mini-review aims to summarize our recent data and research by ot...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ygcen.2007.04.005

    authors： Kozicz T

    更新日期：2007-08-01 00:00:00

  • Changes in parathyroid hormone-related peptide receptor binding in the shell gland of laying hens (Gallus domesticus) during the oviposition cycle.

    abstract：:In this study, parathyroid hormone (PTH) receptor binding was examined in the membrane fraction of the endometrium of the shell gland of laying and nonlaying hens by the use of (125)I-PTH-related peptide (PTHrP) binding assays. Specific (125)I-PTHrP binding was found in the endometrium of the shell gland and increased...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1999.7395

    authors： Ieda T,Takahashi T,Saito N,Yasuoka T,Kawashima M,Shimada K

    更新日期：2000-02-01 00:00:00

  • Calcitonin gene-related peptide in the bullfrog, Rana catesbeiana: localization and vascular actions.

    abstract：:Calcitonin gene-related peptide (CGRP) was found using radioimmunoassay in the brain and spinal cord of the bullfrog, Rana catesbeiana. The brain extracts were found to contain 241.7 +/- 68.1 pg/mg tissue, and the spinal cord contained 1753.0 +/- 96.8 pg/mg tissue. An intense immunocytochemical reaction was observed i...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(88)90187-6

    authors： Kline LW,Kaneko T,Chiu KW,Harvey S,Pang PK

    更新日期：1988-10-01 00:00:00

  • Clarification of emu serum for peptide hormone assay using polyethylene glycol precipitation.

    abstract：:Interference in radioimmunoassays (RIAs) was frequently encountered during endocrinological studies of the emu (Dromaius novaehollandiae). Interference was greatest when serum was cloudy or opaque. Such samples appeared seasonally, in spring and summer during the phase of fat deposition, and in the winter when females...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/s0016-6480(03)00088-1

    authors： Van Cleeff JK,Blackberry MA,Blache D,Martin GB

    更新日期：2003-06-15 00:00:00

  • The role of ubiquitin-proteasome system in ageing.

    abstract：:Maintenance of cellular homeostasis influences ageing and it is determined by several factors, including efficient proteolysis of damaged proteins. The ubiquitin-proteasome system is the major protein degradation pathway in the cell. Specifically, the proteasome is responsible for clearance of abnormal, denatured or i...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ygcen.2011.02.005

    authors： Löw P

    更新日期：2011-05-15 00:00:00

  • Effect of human presence and handling on circulating corticosterone levels in breeding blue tits (Parus caeruleus).

    abstract：:Birds may react to the presence of humans with an immediate primary behavioural reaction and with physiological responses, such as the activation of the hypothalamo-pituitary-adrenal axis. This study investigates the suite of behavioural and adrenocortical responses to the presence of humans and to handling in two sub...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2006.02.012

    authors： Müller C,Jenni-Eiermann S,Blondel J,Perret P,Caro SP,Lambrechts M,Jenni L

    更新日期：2006-09-01 00:00:00

  • The role of hormone binding in the cold suppression of hormone stimulation of the pituitary, thyroid, and testis of the turtle.

    abstract：:The ability of hormones to bind to their functional receptors on turtle (Pseudemys scripta) endocrine target tissues in the cold was tested by treating tissues with secretagogues at low temperatures (5-15 degrees) and then following subsequent target stimulation in the absence of secretagogue at a warm temperature (28...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(90)90187-q

    authors： Licht P,Pavgi S,Denver RJ

    更新日期：1990-12-01 00:00:00

  • Baseline and stress-induced glucocorticoid concentrations are not repeatable but covary within individual great tits (Parus major).

    abstract：:In evolutionary endocrinology, there is a growing interest in the extent and basis of individual variation in endocrine traits, especially circulating concentrations of hormones. This is important because if targeted by selection, such individual differences present the opportunity for an evolutionary response to sele...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2014.08.014

    authors： Baugh AT,van Oers K,Dingemanse NJ,Hau M

    更新日期：2014-11-01 00:00:00

  • Validation of an enzyme-immunoassay for the non-invasive monitoring of faecal testosterone metabolites in male cheetahs (Acinonyx jubatus).

    abstract：:In mammals, the sex hormone testosterone is the major endocrine variable to objectify testicular activity and thus reproductive function in males. Testosterone is involved in the development and function of male reproductive physiology and sex-related behaviour. The development of a reliable androgen enzyme-immunoassa...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/j.ygcen.2016.01.015

    authors： Pribbenow S,Wachter B,Ludwig C,Weigold A,Dehnhard M

    更新日期：2016-03-01 00:00:00

  • The regulation of prolactin cells in the rainbow trout (Oncorhynchus mykiss). 1. Possible roles for thyrotropin-releasing hormone (TRH) and oestradiol.

    abstract：:The regulation of rainbow trout prolactin (PRL) cells by hypothalamic and humoral factors has been investigated. PRL cell activity was assessed using acid gel electrophoresis to measure PRL release in vitro and image analysis to measure PRL cell nuclear area, an index of synthetic activity. 17 beta-oestradiol stimulat...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1006/gcen.1994.1043

    authors： Williams AJ,Wigham T

    更新日期：1994-03-01 00:00:00

  • Weekly variations in body weight and plasma testosterone concentrations in the captive male possum, Trichosurus vulpecula.

    abstract：:The possum is a seasonally breeding marsupial which gives birth in Queensland from March through to September. To ascertain whether this seasonality in breeding is manifest in the male reproductive system, body weight and plasma testosterone concentrations were examined in five possums. Plasma testosterone concentrati...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章

    doi：10.1016/0016-6480(86)90087-0

    authors： Gemmell RT,Cepon G,Barnes A

    更新日期：1986-04-01 00:00:00

  • Pheromone biosynthesis activating neuropeptide (PBAN): regulatory role and mode of action.

    abstract：:This review focuses on the endocrine regulation of reproductive behavior in moth species with particular emphasis on Helicoverpa spp. Reproductive behavior in most adult moths is dependent on the release of a unique blend of sex pheromones by the females to attract conspecific males. Mating, on the other hand, results...

    journal_title：General and comparative endocrinology

    pub_type： 杂志文章,评审

    doi：10.1016/j.ygcen.2008.04.004

    authors： Rafaeli A

    更新日期：2009-05-15 00:00:00