Abstract：
:Interference in radioimmunoassays (RIAs) was frequently encountered during endocrinological studies of the emu (Dromaius novaehollandiae). Interference was greatest when serum was cloudy or opaque. Such samples appeared seasonally, in spring and summer during the phase of fat deposition, and in the winter when females were laying. These poor quality samples did not allow accurate measurement by RIAs of several peptide hormones for a full year. To prepare them for assay, these sera were clarified using a polyethylene glycol (PEG) solution at a final concentration of 7.5%. This treatment was effective in most cases. After treatment with the PEG, recoveries of LH, glucagon, and prolactin were greater than 75% and that for insulin was 40%. Regardless of the level of recovery, there was a high correlation of assay results between non-opaque native and PEG-treated sera. Serum samples containing large amounts of interfering high molecular weight components, such as lipoproteins, can be clarified with PEG, enabling their accurate measurement by RIA.
journal_name
Gen Comp Endocrinoljournal_title
General and comparative endocrinologyauthors
Van Cleeff JK,Blackberry MA,Blache D,Martin GBdoi
10.1016/s0016-6480(03)00088-1keywords:
subject
Has Abstractpub_date
2003-06-15 00:00:00pages
315-20issue
2eissn
0016-6480issn
1095-6840pii
S0016648003000881journal_volume
132pub_type
杂志文章abstract：:In goldfish, plasma gonadotropin levels increase during spawning in both males and females (GTH surge). A female-typical GTH surge induces ovulation (ovulatory surge), and a male-typical surge triggers milt production in response to sex pheromones released from ovulatory females. This study examined whether the male-t...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1997.6993
更新日期：1997-12-01 00:00:00
abstract：:Anthropogenic emissions of carbon dioxide in the atmosphere have generated rapid variations in atmospheric composition which drives major climate changes. Climate change related effects include changes in physico-chemical proprieties of sea and freshwater, such as variations in water temperature, salinity, pH/pCO2 and...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2020.113439
更新日期：2020-05-15 00:00:00
abstract：:Thyroid hormones (THs) are crucial for brain development and maturation in all vertebrates. Especially during pre- and perinatal development, disruption of TH signaling leads to a multitude of neurological deficits. Many animal models provided insight in the role of THs in brain development, but specific data on how t...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2017.04.001
更新日期：2017-06-01 00:00:00
abstract：:Proper functioning of the endocrine stress axis requires communication between the stress axis and other regulatory mechanisms. We here describe an intimate interplay between the stress axis and recombinant human leptin (rhLeptin) in a teleostean fish, the common carp Cyprinus carpio. Restraint stress (by netting up t...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2012.04.004
更新日期：2012-08-01 00:00:00
abstract：:We describe the reproductive cycle of Northern Pacific rattlesnakes (Crotalus oreganus) by quantifying steroid hormone concentrations and observing reproductive behaviors in free-ranging individuals. Additionally, we examined reproductive tissues from museum specimens. Plasma steroid hormone concentrations were quanti...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.01.026
更新日期：2010-05-01 00:00:00
abstract：:The Hexapoda consists of two classes, the Entognatha and the Insecta, with the former group considered basal to the latter. The Protura is a basal order within the Entognatha, the members of which are minute soil dwellers first identified in the early 20th century. Recently, a transcriptome shotgun assembly (TSA) was ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2015.05.015
更新日期：2015-12-01 00:00:00
abstract：:In mammals ghrelin has a diverse range of effects including stimulation of gut motility but although present in teleost fish its effects on motility have not been investigated. The present study used bioinformatics to search for fish paralogues of the ghrelin receptor and the closely related motilin receptor, and inve...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2007.05.016
更新日期：2008-01-01 00:00:00
abstract：:The aim of this work was to study, in the Cebus apella monkey, the developmental changes in the microanatomy of the utero-ovarian ligament (UOL) and whether their vascular and neural elements might be involved in the transfer of signals between the ovaries and uterus. Sections including uterus, UOL, and ovary obtained...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2005.06.006
更新日期：2005-12-01 00:00:00
abstract：:Our previous study demonstrated that recombinant goldfish activin B stimulated goldfish FSHbeta but inhibited LHbeta expression. Similar to activin B, activin A also exhibited the inverse effects on the expression of the two gonadotropins. The novel dual effects of activins on FSH and LH in the goldfish raise an inter...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2003.08.012
更新日期：2004-01-01 00:00:00
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journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.2000.7561
更新日期：2001-01-01 00:00:00
abstract：:The effect of salmon calcitonin (0.25 MRC mU/g body wt) was investigated on the serum calcium and inorganic phosphate levels of the frog. Rana tigrina. The hormone evokes hypocalcemia (on Day 1 and Day 3) which is followed by a significant hypercalcemia on Day 10. Thereafter, the level of calcium decreases again on Da...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(89)90109-3
更新日期：1989-04-01 00:00:00
abstract：:The circulating serum concentrations of various steroid hormones in mature sockeye salmon were measured at four different developmental stages in their upstream migration to spawn at Adams River in British Columbia, Canada. In females, a high level of estradiol-17 beta was found in fish at the first location, and it p...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(86)90098-5
更新日期：1986-04-01 00:00:00
abstract：:This review focuses on the endocrine regulation of reproductive behavior in moth species with particular emphasis on Helicoverpa spp. Reproductive behavior in most adult moths is dependent on the release of a unique blend of sex pheromones by the females to attract conspecific males. Mating, on the other hand, results...
journal_title：General and comparative endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.ygcen.2008.04.004
更新日期：2009-05-15 00:00:00
abstract：:Two experiments were conducted to further examine the interaction of social stimuli and photoperiod on cloacal and gonadal responses in male Japanese quail. In experiment 1, adult males kept on a maximal (15L:9D) stimulatory photoperiod were surgically deafened and/or visually isolated from roommates. After 6 weeks, t...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(88)90037-8
更新日期：1988-03-01 00:00:00
abstract：:Vitellogenesis in tuatara (Sphenodon punctatus) on Stephens Island, New Zealand, at the southern (coolest) end of the geographical range of this species involves a prolonged period of about 3 years of the average 4-year reproductive cycle. These studies used a semiquantitative electrophoretic assay for vitellogenin (V...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1994.1117
更新日期：1994-08-01 00:00:00
abstract：:Seasonal and daily variation in basal plasma concentrations of corticosterone and the effect of short-term confinement on plasma corticosterone concentration were examined in wild adult male and female tuatara (Sphenodon punctatus) on Stephens Island, New Zealand. Males and females sampled immediately upon capture at ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1997.7051
更新日期：1998-05-01 00:00:00
abstract：:Effects of adrenal corticosteroids on reproductive and endocrine functions of the gonads are well known, but reciprocal effects of gonadal hormones on the hypothalamo-pituitary-adrenal (HPA) axis and on adrenocortical steroidogenesis in particular have received much less attention. We investigated effects of gonadecto...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2008.07.018
更新日期：2008-09-01 00:00:00
abstract：:Male bluegill (Lepomis macrochirus) display a complex reproductive behavior involving two alternative life history pathways: delay of sexual maturation to become "parentals" or precocious maturation as "cuckolders." The purpose of our study was to investigate the association of two androgens, 11-ketotestosterone (11KT...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(89)90180-9
更新日期：1989-09-01 00:00:00
abstract：:Growth hormone-releasing hormone (GHRH) plays a pivotal role in the regulation of growth. The study of goldfish GHRH and its receptor is of particular interest as it is so far the only animal model in which two forms of GHRH-like (catfish-like and salmon-like) peptides coexist, and these peptides share only 30-40% of ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2004.09.010
更新日期：2005-01-01 00:00:00
abstract：:The role of ovarian hormones in the expression of parental behavior and in the regulation of LH secretion was investigated in incubating commercial meat-type hens. After ovariectomy, incubating hens continued to incubate eggs normally and brooded day-old chicks given to replace eggs, in a manner similar to sham-ovarie...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1996.0013
更新日期：1996-01-01 00:00:00
abstract：:Leptin, a 16-kDa hormone produced by mature adipocytes, has been shown to regulate the hibernation of mammals. In this study, the leptin gene sequences of both hibernating (Rhinolophus ferrumequinum) and non-hibernating (Rousettus leschenaultii) bats were determined, and the leptin proteins from these two different sp...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.04.001
更新日期：2010-08-01 00:00:00
abstract：:The brain of many teleost fish species, including the goldfish Carassius auratus, expresses exceptionally high levels of cytochrome P450 aromatase (estrogen synthetase). To begin investigating the molecular and cellular targets of estrogen action in goldfish brain, a polymerase chain reaction (PCR) cloning strategy wa...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1006/gcen.1998.7217
更新日期：1999-03-01 00:00:00
abstract：:Age-related changes in the concentration of factors like TGF-1β, DHEA-S and IGF-1 may increase the risk of disease and illnesses in advanced life. A better understanding of these changes would aid in the development of more appropriate treatments and/or preventative care for many conditions associated with age. Due to...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2013.10.004
更新日期：2014-01-01 00:00:00
abstract：:Effects of fasting on the growth hormone (GH)--growth hormone receptor (GHR)-insulin-like growth factor-I (IGF-I) axis were characterized in seawater-acclimated tilapia (Oreochromis mossambicus). Fasting for 4 weeks resulted in significant reductions in body weight and specific growth rate. Plasma GH and pituitary GH ...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2006.04.007
更新日期：2006-09-15 00:00:00
abstract：:The Lottia gigantea genome was prospected for the presence of genes coding neuropeptides and neurohormones. Four genes code insulin-related peptides: two genes code molluscan insulin-like growth hormones, one gene an insulin very similar to vertebrate insulin, and the fourth a peptide related to drosophila insulin-lik...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2010.02.010
更新日期：2010-05-15 00:00:00
abstract：:The insulin/PI3K/Akt signaling pathway is strongly involved in the differentiation of C2C12 cells, as has been demonstrated by the addition of IGFs and insulin to culture media. In this study, we have characterized the role of insulin in chick myoblast proliferation and differentiation in vitro and in vivo, and have r...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2011.11.043
更新日期：2012-02-01 00:00:00
abstract：:The isolation of a maturational gonadotropin from pituitaries of Atlantic croaker (Micropogonias undulatus), a marine teleost belonging to the order perciformes, is described. A radioimmunoassay has been developed for the measurement of this hormone in blood samples and validation of the assay is presented. The hormon...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(89)90200-1
更新日期：1989-03-01 00:00:00
abstract：:We have studied the effects of bovine parathyroid hormone (PTH) and salmon calcitonin (CT) on plasma calcium (Ca) and phosphate levels and on renal function in intact and parathyroidectomized (PTX) chickens, using a simple technique that separates urine from feces. In immature males and egg-laying females, the intrave...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(87)90248-6
更新日期：1987-06-01 00:00:00
abstract：:Low birth weight is correlated with low adiposity at birth, a phenotype that influences neonatal survival and later adiposity. A better understanding of events affecting the fetal adipose tissue development and its functionality around birth is thus needed. This study was undertaken to examine the impact of spontaneou...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/j.ygcen.2013.09.018
更新日期：2013-12-01 00:00:00
abstract：:The purpose of this study was to examine the effect of the daily infusion of corticosterone on reproductive function in the laying hen and to determine the relationship between the cyclic pattern of plasma concentrations of corticosterone on the open-period for the preovulatory release of LH. An exogenous rhythm of pl...
journal_title：General and comparative endocrinology
pub_type： 杂志文章
doi：10.1016/0016-6480(91)90145-v
更新日期：1991-09-01 00:00:00
