Apposition of enkephalinergic axons with cGnRH I-containing perikarya in turkey hen brain.

Abstract:

:Several lines of evidence support a role of endogenous opioids in the brain's regulation of gonadotropin secretion in birds and mammals, although the neuroanatomic basis of such regulation is not clear. We used double-label fluorescence immunohistochemistry employing polyclonal antisera raised in sheep against chicken gonadotropin-release hormone I (cGnRH I) and in rabbits against met-enkephalin to determine whether the potential exists for synaptic contact between neurons containing these neuropeptides in the preoptic/septal region of domestic turkey breeder hens. The cGnRH I antiserum was highly specific for cGnRH I, while the met-enkephalin antiserum showed some cross-reactivity with leu-enkephalin. We found numerous instances in which enkephalinergic neuronal elements appeared to contact cGnRH I perikarya and axons in and around the diagonal band of Broca, the bed nucleus of the pallial commissure and in the lateral septal nucleus. These appositions, confirmed by confocal scanning microscopy, appear to provide a neuroanatomic basis for how enkephalinergic innervation might influence gonadotropin secretion in turkey hens by directly regulating activity of cGnRH I neurons.

journal_name

Gen Comp Endocrinol

authors

Millam JR,Wang R,Craig-Veit CB,Siopes TD

doi

10.1016/s0016-6480(02)00029-1

keywords:

subject

Has Abstract

pub_date

2002-06-15 00:00:00

pages

128-35

issue

2

eissn

0016-6480

issn

1095-6840

pii

S0016648002000291

journal_volume

127

pub_type

杂志文章