Abstract:
:A crossed radioimmunoelectrophoretic (CRIE) method for detection of human IgG subclass specificities against individual antigens is described. After crossed immunoelectrophoresis (CIE) of the antigens the immunoplates are incubated with serum, washed and incubated with mouse monoclonal anti-human IgG-subclass antibodies. After washing, the plates are finally incubated with the 125I-labelled detector protein, rabbit anti-mouse IgG, and washed. The plates are then placed on an X-ray film for autoradiography. The specificity of the method was tested by inhibition with antigens in the first layer and by inhibition with myeloma sera containing only one IgG subclass protein in the second layer. The specificity of the third layer was assured by affinity purification of the rabbit anti-mouse IgG antibodies. The method was shown to be sensitive and specific. The test systems were timothy (Phleum pratense) pollen antigens and house dust mite (Dermatophagoides pteronyssinus) antigens.
journal_name
Allergyjournal_title
Allergyauthors
Søndergaard I,Djurup R,Weeke Bdoi
10.1111/j.1398-9995.1984.tb01983.xsubject
Has Abstractpub_date
1984-11-01 00:00:00pages
622-9issue
8eissn
0105-4538issn
1398-9995journal_volume
39pub_type
杂志文章相关文献
ALLERGY文献大全abstract:BACKGROUND:Multiple drug hypersensitivity syndrome (MDH) is used to describe persons with a drug hypersensitivity reaction (DHR) to at least two chemically unrelated drugs, confirmed by skin test or in vitro assay. METHODS:Medical records of 25 patients with MDH, tested and confirmed at our allergy division, were retr...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.14146
更新日期:2020-05-01 00:00:00
abstract:BACKGROUND:In cross-sectional clinical studies, rhinitis has been shown to be strongly associated with co-existing chronic cough. However, to date, this association has been poorly delineated from a prospective and epidemiological standpoint. METHODS:We used data from the 'Pisa Prospective Study', a population-based l...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2005.00717.x
更新日期:2005-03-01 00:00:00
abstract:BACKGROUND:Occupational allergy forms an attractive model to study the development of allergic responses, as in some occupations it has a high incidence and develops quickly. In a cohort of starting laboratory animal workers, we previously found 20% sensitization to animal allergens within 2 years. METHODS:We compared...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2009.02323.x
更新日期:2010-10-01 00:00:00
abstract:BACKGROUND:Subjective symptoms after food challenges are difficult to interpret and no standard is available. We discuss a strategy for how to interpret a diary. Furthermore, the interobserver reliability is evaluated. METHODS:Diaries for 32 patients with subjective symptoms were used. The diaries were re-evaluated wi...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2003.00437.x
更新日期:2004-03-01 00:00:00
abstract::The usefulness of the leukocyte migration inhibition factor (LIF) test to detect cow milk (CM) hypersensitivity was studied in 40 children with suspected allergy to CM. Hypersensitivity was carefully investigated by oral milk challenges, which gave a final confirmation of cow milk hypersensitivity in 12 subjects, and ...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1987.tb00392.x
更新日期:1987-11-01 00:00:00
abstract::An affinity chromatography method for preparation of patient-related antigens from commercially available allergen extracts has been investigated. IgG1,2,4 from a patient previously hyposensitized with dog hair and dandruff allergen was bound to protein A-sepharose. Secondly, commercial allergen extract was applied to...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1988.tb00927.x
更新日期:1988-11-01 00:00:00
abstract::Climate therapy has been used for decades in the treatment of atopic dermatitis (AD), but evidence of its effectiveness has not yet been assessed systematically. A systematic literature search in Medline, Embase, and the Cochrane library was performed to identify all original studies concerning alpine climate treatmen...
journal_title:Allergy
pub_type: 杂志文章,meta分析,评审
doi:10.1111/all.12514
更新日期:2015-01-01 00:00:00
abstract:BACKGROUND:In high-risk populations, allergen-specific prophylaxis could protect from sensitization and subsequent development of allergic disease. However, such treatment might itself induce sensitization and allergies, thus requiring hypoallergenic vaccine formulations. We here characterized the preventive potential ...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.13573
更新日期:2019-02-01 00:00:00
abstract::PVG rats given injections of 1 microgram ovalbumin (OA) together with 10 mg Silica gel failed to provide serosal mast cells or lung tissue with the capacity to release histamine on in vitro challenge with the antigen. However, if such animals were injected i.p. with 100 mg of alum (without any further antigen addition...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1983.tb01618.x
更新日期:1983-05-01 00:00:00
abstract:BACKGROUND:Hippo-Yes-associated protein (YAP) pathway plays an important role in epithelial cell proliferation and development. However, its possible role in chronic rhinosinusitis with nasal polyps (CRSwNP) remains unknown. We aim to investigate it on nasal epithelial proliferation and remodeling in CRSwNP. METHODS:T...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.13647
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Several epidemiologic studies in the United States and Europe have linked Alternaria sensitivity to both persistence and severity of asthma. In this study, we examined T cell responses and HLA class II alleles in children with moderate-severe asthma. METHODS:Ninety-six children with moderate-severe asthma w...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2010.02382.x
更新日期:2010-11-01 00:00:00
abstract:BACKGROUND:Measurement of exposure to the dust mite allergen Der p 1 is important in asthma research and is potentially useful in managing asthma. As no single measure can capture all characteristics of an exposure, it is important to recognize differences in the available methods of measuring exposure to Der p 1. MET...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1034/j.1398-9995.2003.00228.x
更新日期:2003-08-01 00:00:00
abstract::Quantitative determinations of the inflammatory mediators in nasal secretions were performed and correlated with the objective nasal symptoms within 1 h after nasal allergen challenge (NAC). Twenty-six patients with seasonal allergic rhinitis were enrolled outside the pollen season. All measurements were performed bef...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1997.tb00970.x
更新日期:1997-02-01 00:00:00
abstract::The association between food allergy and celiac disease (CD) is still to be clarified. We screened for CD 319 patients with severe food allergy (IgE > 85 kU/l against food proteins and a history of severe allergic reactions) who underwent specific food oral immunotherapy (OIT), together with 128 children with mild all...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.12692
更新日期:2015-10-01 00:00:00
abstract:BACKGROUND:The effectiveness of acaricides in homes is controversial. OBJECTIVE:To determine whether disodium octaborate tetrahydrate (DOT) combined with vacuuming lowers dust mite numbers and their allergens in carpets and sofas. METHODS:A 6-month study was carried out with 93 homes, which were randomized into three...
journal_title:Allergy
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1034/j.1398-9995.2003.00100.x
更新日期:2003-04-01 00:00:00
abstract::The use of recombinant DNA techniques for the study of allergenicity of proteins is a viable, and in many ways a preferred, alternative to the traditional procedures of protein purification, digestion and analysis of peptides for both allergenicity and amino acid sequence. The process of protein purification can be di...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1111/j.1398-9995.1989.tb02230.x
更新日期:1989-02-01 00:00:00
abstract::Skin tests and estimation of specific IgE-antibodies by the RAST are evaluated as diagnostic procedures in bee sting hypersensitivity with the followiny extracts. (2) With bee venom results of skin tests and RAST correspond in 82%, with bee wholebody extracts in 68%. (3) Both with skin tests and the RAST with bee veno...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1979.tb01705.x
更新日期:1979-08-01 00:00:00
abstract:BACKGROUND:Allergy to pollen from gymnosperms is well documented in the West. However, many allergenic species are native to the Himalayan region of India, and Cedrus deodara (Pinaceae) was selected for allergologic investigation. The objective was to define the allergologic and immunochemical aspects of C. deodara pol...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1034/j.1398-9995.2000.00491.x
更新日期:2000-07-01 00:00:00
abstract:BACKGROUND:A previous investigation of all 10 TLR genes for associations with allergic rhinitis (AR) detected a number of significant SNPs in the TLR8 locus. The associations indicated that an accumulation of rare variants could explain the signal. This study therefore searches for rare variants in the TLR8 region and ...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.12805
更新日期:2016-03-01 00:00:00
abstract:BACKGROUND:Exposure to acrylates may give rise to rhinitis and asthma in both industrial and domestic environments. The mechanisms underlying these respiratory conditions caused by acrylates remain largely unknown. METHODS:We studied two assembly operators exposed to cyanoacrylate glue who developed rhinitis and asthm...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1034/j.1398-9995.2001.056005446.x
更新日期:2001-05-01 00:00:00
abstract::Formoterol and salmeterol are 2 newly developed beta 2-adrenoceptor agonists for inhalation with prolonged duration of effect compared with currently available beta 2-agonists. The clinical duration of effect has been suggested to be correlated to a decreased washability in vitro, i.e. decreased effect due to continuo...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1992.tb02076.x
更新日期:1992-08-01 00:00:00
abstract:BACKGROUND:To date, there has been no reliable in vitro test to either diagnose or differentiate nonsteroidal anti-inflammatory drug (NSAID)-exacerbated respiratory disease (N-ERD). The aim of the present study was to develop and validate an artificial neural network (ANN) for the prediction of N-ERD in patients with a...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.14214
更新日期:2020-07-01 00:00:00
abstract::In 77 consecutive patients with grass pollen rhinitis as the only allergic disease, treatment with sodium cromoglycate (SCG) nasal spray, hyposensitization with aluminium hydroxide adsorbed extract of grass pollen as perennial treatment, and a combination of the two was compared. Specific serum-IgE, IgG and IgG4 were ...
journal_title:Allergy
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1398-9995.1987.tb02220.x
更新日期:1987-07-01 00:00:00
abstract::In analogy with findings from animal experiments, people with low glutathione-peroxidase (GSH-Px) activity could be expected to have altered sensitivities to effects of drugs, chemicals and possibly food. We have investigated GSH-Px activity in 12 patients with intrinsic asthma and food and aspirin intolerance. Ten of...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1986.tb00273.x
更新日期:1986-01-01 00:00:00
abstract::Interleukin-33 (IL-33) is a member of the IL-1 cytokine family, which includes IL-1 and IL-18, and is considered to be important for host defense against nematodes by inducing Th2 cytokine production via the IL-33 receptor. IL-33 receptor is a heterodimer of IL-1 receptor-like 1 (IL-1RL1; also called ST2, T1, Der4, an...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1111/all.12004
更新日期:2012-10-01 00:00:00
abstract:BACKGROUND:Food allergy in children can be life-threatening. Component-resolved diagnostics approach to food anaphylaxis is rarely assessed in children. The aim of the study was to identify the food allergen components as the triggers responsible for severe anaphylaxis, with regard to characteristics and associated ris...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.13810
更新日期:2019-10-01 00:00:00
abstract:BACKGROUND:Mast cells have recently been shown to control neutrophil recruitment during T-cell mediated cutaneous DTH reaction in vivo through TNF-alpha and MIP-2, the functional murine analogue of human IL-8. Although the nature of signals transmitted from T cells which activate mast cells has not yet been defined, we...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2005.00886.x
更新日期:2005-10-01 00:00:00
abstract:BACKGROUND:Reasons for asthma hospitalizations are dynamic and complex. Comorbid conditions are important contributors to most chronic diseases today. We aim to characterize and describe risk factors associated with hospitalizations due to asthma in the Languedoc-Roussillon region (France) in 2009. METHODS:Programme d...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.12137
更新日期:2013-01-01 00:00:00
abstract:BACKGROUND:Little is known about the diagnostic approaches for immediate hypersensitivity reactions (IHRs) due to 5-nitroimidazole antibiotics. The aim was to evaluate the usefulness of in vivo tests and basophil activation test (BAT) for the diagnosis of IHRs due to metronidazole and ornidazole and to determine possib...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.14542
更新日期:2020-08-05 00:00:00
abstract::The aim of the present investigation was to study the dose response relationship of allergen, histamine and histamine releasers in skin prick test (SPT) and the precision of the SPT method. In one experiment timothy allergen, histamine HCl, rabbit anti-human-IgE and compound 48/80 were studied in seven patients. In a ...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1987.tb02369.x
更新日期:1987-02-01 00:00:00