Preparation of patient-related allergens for hyposensitization. Qualitative aspects.


:An affinity chromatography method for preparation of patient-related antigens from commercially available allergen extracts has been investigated. IgG1,2,4 from a patient previously hyposensitized with dog hair and dandruff allergen was bound to protein A-sepharose. Secondly, commercial allergen extract was applied to the immunosorbent, and patient-related antigens were selectively absorbed by the specific antibodies from the patient serum. Finally, immune complexes containing antigen and IgG were eluted. Alternatively, the antigens alone were purified by retaining the IgG on the column by means of a covalent reinforcement of the protein A-IgG-binding. Purified, patient-related antigens were investigated in crossed immunoelectrophoresis and identity to IgG- and IgE-binding antigens (as determined by the CRIE-technique) was suggested. The affinity purified IgG was unaltered with regard to protein A-binding, binding of antigen and affinity for monoclonal antibody to human IgG-subclasses. Further, it was demonstrated that the antigen-binding capacity of IgG in the immune complexes was intact as evidenced by strong affinity to antigens even at low pH. The antigens eluted together with IgG were predominantly found in immune complexes with a molecular weight of greater than 300 kdalton equivalent to 2 or more molecules of IgG. The possibility of employing a similar method with IgE instead of IgG for preparation of patient-related allergens instead of antigens, is discussed.






Poulsen LK,Søndergaard I,Weeke B




Has Abstract


1988-11-01 00:00:00














  • Lifestyle and parental allergen sensitization are reflected in the intrauterine environment at gene expression level.

    abstract:BACKGROUND:Environmental factors, including the intrauterine environment, can influence the risk of allergy development. In the present study, we investigated whether lifestyle and parental allergen sensitization status are reflected at gene expression level in the intrauterine environment. METHODS:  mRNA expression o...


    pub_type: 杂志文章


    authors: Joerink M,Oortveld MA,Stenius F,Rindsjö E,Alm J,Scheynius A

    更新日期:2010-10-01 00:00:00

  • Design, engineering and in vitro evaluation of MHC class-II targeting allergy vaccines.

    abstract:BACKGROUND:The worldwide increasing incidence of allergic diseases requires the development of new, efficient vaccination strategies, the only curative treatment with a long-lasting effect. Current allergen-specific immunotherapy protocols suffer from limited efficacy and a long treatment time. METHODS:We engineered m...


    pub_type: 杂志文章


    authors: Crameri R,Flückiger S,Daigle I,Kündig T,Rhyner C

    更新日期:2007-02-01 00:00:00

  • Airway inflammation in asthma and perennial allergic rhinitis. Relationship with nonspecific bronchial responsiveness and maximal airway narrowing.

    abstract:BACKGROUND:Eosinophilic airway inflammation is the hallmark of asthma, but it has also been reported in other conditions such as allergic rhinitis. We have tested whether the analysis of cells and chemicals in sputum can distinguish between patients with mild allergic asthma, those with allergic rhinitis, and healthy c...


    pub_type: 杂志文章


    authors: Alvarez MJ,Olaguibel JM,García BE,Rodríquez A,Tabar AI,Urbiola E

    更新日期:2000-04-01 00:00:00

  • Undernotification of anaphylaxis deaths in Brazil due to difficult coding under the ICD-10.

    abstract:BACKGROUND:Undernotification is well recognized as a key challenge to the study of anaphylaxis mortality, but it is seldom mentioned that one of its reasons is the difficult coding of the condition under the tenth revision of the international classification of diseases (ICD-10), given that there are no anaphylaxis-spe...


    pub_type: 杂志文章


    authors: Tanno LK,Ganem F,Demoly P,Toscano CM,Bierrenbach AL

    更新日期:2012-06-01 00:00:00

  • Phthalates suppress type I interferon in human plasmacytoid dendritic cells via epigenetic regulation.

    abstract:BACKGROUND:Exposure to environmental endocrine-disrupting chemicals (EDCs) is associated with allergy, chronic inflammation, and immunodeficiency. Phthalates, the common EDCs used in plastic industry, may act as adjuvants to disrupt immune system and enhance allergy. Plasmacytoid DCs (pDCs) are predominant cells secret...


    pub_type: 杂志文章


    authors: Kuo CH,Hsieh CC,Kuo HF,Huang MY,Yang SN,Chen LC,Huang SK,Hung CH

    更新日期:2013-07-01 00:00:00

  • Prevalence of celiac disease in patients with severe food allergy.

    abstract::The association between food allergy and celiac disease (CD) is still to be clarified. We screened for CD 319 patients with severe food allergy (IgE > 85 kU/l against food proteins and a history of severe allergic reactions) who underwent specific food oral immunotherapy (OIT), together with 128 children with mild all...


    pub_type: 杂志文章


    authors: Pillon R,Ziberna F,Badina L,Ventura A,Longo G,Quaglia S,De Leo L,Vatta S,Martelossi S,Patano G,Not T,Berti I

    更新日期:2015-10-01 00:00:00

  • Biologic activity of Dermatophagoides siboney and Blomia tropicalis allergens in exposed and unexposed mite-allergic individuals. Effect of patient selection on the biologic standardization of mite extracts.

    abstract:BACKGROUND:This study aimed to investigate the influence of patient selection criteria, i.e., mite-allergic individuals exposed and not exposed to Dermatophagoides siboney and Blomia tropicalis, on the biologic activity of mite extracts. Determination of the potency of mite extracts in vivo requires selection of patien...


    pub_type: 杂志文章


    authors: Casas R,Ferrándiz R,Wihl JA,Fernández B,Dreborg S

    更新日期:1999-04-01 00:00:00

  • Hypersensitivity to cefuroxime with good tolerance to other betalactams.

    abstract::A 60-year-old man developed an anaphylactic reaction immediately after administration of intramuscular sodium cefuroxime. By means of oral controlled challenge, the reaction was reproduced with a smaller dose of cefuroxime axetil. Cross-reactivity with other betalactam antibiotics was ruled out by in vivo tests. This ...


    pub_type: 杂志文章


    authors: Marcos Bravo C,Luna Ortiz I,González Vázquez R

    更新日期:1995-04-01 00:00:00

  • Volumetric aerobiological survey of conidial fungi in the North-East Netherlands. II. Comparison of aerobiological data and skin tests with mould extracts in an asthmatic population.

    abstract::A study was undertaken to see whether the principal airborne fungi in the North-East Netherlands were also found to be the most reactive in skin testing. Atmospheric samples were taken weekly with the Andersen sampler, from April 1981, up to and including, June 1983. At the same time skin tests of 833 patients referre...


    pub_type: 杂志文章


    authors: Beaumont F,Kauffman HF,de Monchy JG,Sluiter HJ,de Vries K

    更新日期:1985-04-01 00:00:00

  • Cost-effectiveness of asthma control: an economic appraisal of the GOAL study.

    abstract:BACKGROUND:The Gaining Optimal Asthma ControL (GOAL) study has shown the superiority of a combination of salmeterol/fluticasone propionate (SFC) compared with fluticasone propionate alone (FP) in terms of improving guideline defined asthma control. METHODS:Clinical and economic data were taken from the GOAL study, sup...


    pub_type: 杂志文章,多中心研究,随机对照试验


    authors: Briggs AH,Bousquet J,Wallace MV,Busse WW,Clark TJ,Pedersen SE,Bateman ED,GOAL Investigators Group.

    更新日期:2006-05-01 00:00:00

  • The role of ORMDL proteins, guardians of cellular sphingolipids, in asthma.

    abstract::A family of widely expressed ORM-like (ORMDL) proteins has been recently linked to asthma in genomewide association studies in humans and extensively explored in in vivo studies in mice. ORMDL proteins are key regulators of serine palmitoyltransferase, an enzyme catalyzing the initial step of sphingolipid biosynthesis...


    pub_type: 杂志文章,评审


    authors: Paulenda T,Draber P

    更新日期:2016-07-01 00:00:00

  • Prevention of allergy by virus-like nanoparticles (VNP) delivering shielded versions of major allergens in a humanized murine allergy model.

    abstract:BACKGROUND:In high-risk populations, allergen-specific prophylaxis could protect from sensitization and subsequent development of allergic disease. However, such treatment might itself induce sensitization and allergies, thus requiring hypoallergenic vaccine formulations. We here characterized the preventive potential ...


    pub_type: 杂志文章


    authors: Kratzer B,Köhler C,Hofer S,Smole U,Trapin D,Iturri J,Pum D,Kienzl P,Elbe-Bürger A,Gattinger P,Mittermann I,Linhart B,Gadermaier G,Jahn-Schmid B,Neunkirchner A,Valenta R,Pickl WF

    更新日期:2019-02-01 00:00:00

  • A meta-analysis on wood dust exposure and risk of asthma.

    abstract::Work-related asthma is the most common occupational respiratory disorder in the industrialized countries. It has been postulated that wood dust exposure may increase the risk of work-related asthma. The objective of this study was to assess, through a meta-analysis, the risk of developing work-related asthma associate...


    pub_type: 杂志文章,meta分析


    authors: Pérez-Ríos M,Ruano-Ravina A,Etminan M,Takkouche B

    更新日期:2010-04-01 00:00:00

  • Impact of (bio)chemical and physical procedures on food allergen stability.

    abstract::A variety of methods have been used to attempt to decrease the allergenicity of food products, with highly variable success. Limited knowledge of allergen and epitope structures and the factors governing their stability may explain this, and the ensuing need for an empirical approach. A combined effort from a processi...


    pub_type: 杂志文章,评审


    authors: Soler-Rivas C,Wichers HJ

    更新日期:2001-01-01 00:00:00

  • Storage mites dominate the fauna in Swedish barn dust.

    abstract::The mite fauna in hay dust of 16 farms located on the island of Gotland was analysed to establish whether there is a clear relationship between environmental exposure to storage mites and development of allergic symptoms. Eight of these farms belonged to farmers with respiratory symptoms related to barn dust (RS) and ...


    pub_type: 杂志文章


    authors: van Hage-Hamsten M,Johansson E,Wirén A,Johansson SG

    更新日期:1991-02-01 00:00:00

  • Food-induced immediate response of the esophagus-A newly identified syndrome in patients with eosinophilic esophagitis.

    abstract:BACKGROUND:Dysphagia is the main symptom of adult eosinophilic esophagitis (EoE). We describe a novel syndrome, referred to as "food-induced immediate response of the esophagus" (FIRE), observed in EoE patients. METHODS:Food-induced immediate response of the esophagus is an unpleasant/painful sensation, unrelated to d...


    pub_type: 杂志文章


    authors: Biedermann L,Holbreich M,Atkins D,Chehade M,Dellon ES,Furuta GT,Hirano I,Gonsalves N,Greuter T,Gupta S,Katzka DA,De Rooij W,Safroneeva E,Schoepfer A,Schreiner P,Simon D,Simon HU,Warners M,Bredenoord AJ,Straumann A

    更新日期:2021-01-01 00:00:00

  • Allergen-induced mediator release tests.

    abstract::The diagnosis of allergic reactions in clinical practice is based on both clinical history and the determination of specific immunoglobulin E (IgE), either in the serum or on skin mast cells. However, for various reasons, identification of the causative factors is not possible in all the cases. Moreover, not all aller...


    pub_type: 杂志文章,评审


    authors: Demoly P,Lebel B,Arnoux B

    更新日期:2003-07-01 00:00:00

  • Immunostimulatory DNA-based therapeutics for experimental and clinical allergy.

    abstract::Although allergen immunotherapy (IT) has been shown to modulate allergic hypersensitivities, its efficacy is limited. Recently, in various models of experimental allergy, a number of reagents which we have termed immunostimulatory DNA-based therapeutics have proven highly effective in both the prevention and reversal ...


    pub_type: 杂志文章,评审


    authors: Horner AA,Takabaysahi K,Zubeldia JM,Raz E

    更新日期:2002-01-01 00:00:00

  • IgE autoantibodies in atopic dermatitis--occurrence of different antibodies against the CH3 and the CH4 epitopes of IgE.

    abstract::Levels of "free" anti-IgE autoantibodies and IgE/anti-IgE immune complexes were measured in the sera of patients with atopic dermatitis before and after treatment, psoriasis patients, and nonatopic controls. In this measurement, we used two monoclonal antibodies with distinct in vitro functions (LE 27, BSW 17), direct...


    pub_type: 杂志文章


    authors: Czech W,Stadler BM,Schôpf E,Kapp A

    更新日期:1995-03-01 00:00:00

  • Activation of KIT modulates the function of tumor necrosis factor-related apoptosis-inducing ligand receptor (TRAIL-R) in mast cells.

    abstract:BACKGROUND:Mastocytosis is characterized by the accumulation of mast cells (MCs) associated with activating mutations of KIT. Tumor necrosis factor-related apoptosis-inducing ligand receptors (TRAIL-Rs) are preferentially expressed on neoplastic cells and induce the extrinsic apoptotic pathway. Recent studies reported ...


    pub_type: 杂志文章


    authors: Förster A,Grotha SP,Seeger JM,Rabenhorst A,Gehring M,Raap U,Létard S,Dubreuil P,Kashkar H,Walczak H,Roers A,Hartmann K

    更新日期:2015-07-01 00:00:00

  • The utility of monitoring trimellitic anhydride (TMA)-specific IgG to predict IgE-mediated sensitization in an immunosurveillance program.

    abstract:BACKGROUND:Workplace exposure to trimellitic anhydride (TMA) can elicit TMA-specific IgE (sIgE), which may lead to occupational asthma (OA). An occupational immunosurveillance program (OISP) has been implemented to monitor TMA exposure and immunologic outcomes. The purpose of this study was to determine whether TMA-spe...


    pub_type: 杂志文章


    authors: Ghosh D,Clay C,Bernstein JA

    更新日期:2018-05-01 00:00:00

  • Effect of grass pollen immunotherapy on clinical and local immune response to nasal allergen challenge.

    abstract:RATIONALE:Nasal allergen provocations may be useful in investigating the pathophysiology of allergic rhinitis and effects of treatments. OBJECTIVE:To use grass pollen nasal allergen challenge (NAC) to investigate the effects of allergen immunotherapy in a cross-sectional study. METHODS:We studied nasal and cutaneous ...


    pub_type: 杂志文章


    authors: Scadding GW,Eifan AO,Lao-Araya M,Penagos M,Poon SY,Steveling E,Yan R,Switzer A,Phippard D,Togias A,Shamji MH,Durham SR

    更新日期:2015-06-01 00:00:00

  • Allergen sensitization and exposure to irritants in infancy.

    abstract::We investigated the relationship between residence in the neighbourhood of an aluminium smelter and the prevalence of atopy in schoolchildren (7-13 years of age). Atopy was assessed in 556 of the 620 participants by a skin prick test with eight common aeroallergens. The median exposures to sulphur dioxide and fluoride...


    pub_type: 杂志文章


    authors: Søyseth V,Kongerud J,Boe J

    更新日期:1996-10-01 00:00:00

  • New approach shows no association between maternal milk fatty acid composition and childhood wheeze or asthma.

    abstract:BACKGROUND:Previous observational studies have implied breastmilk fatty acid composition may play a role in the development of atopic eczema or atopic sensitization in breastfed infants and toddlers. However, studies investigating associations with wheeze and asthma in later childhood are scarce and did not account for...


    pub_type: 杂志文章


    authors: Logan CA,Brandt S,Wabitsch M,Brenner H,Wiens F,Stahl B,Marosvölgyi T,Decsi T,Rothenbacher D,Genuneit J

    更新日期:2017-09-01 00:00:00

  • Primary care: the cornerstone of diagnosis of allergic rhinitis.

    abstract::Allergic rhinitis (AR) is a very common disease with over 600 million people (200 million of them with concomitant asthma) worldwide suffering from it. The majority of patients who seek medical advice are seen in primary care. Although there is a selection of guidelines focused on the management of AR, there is a pauc...


    pub_type: 杂志文章,评审


    authors: Ryan D,van Weel C,Bousquet J,Toskala E,Ahlstedt S,Palkonen S,van den Nieuwenhof L,Zuberbier T,Wickman M,Fokkens W

    更新日期:2008-08-01 00:00:00

  • Long-term reduction in local inflammation by a lipid raft molecule in atopic dermatitis.

    abstract:BACKGROUND:The complex pathogenesis of atopic dermatitis (AD) is guided by cell surface receptor-mediated signal transduction regulated in lipid rafts. Miltefosine is a raft-modulating molecule targeting cell membranes. With this controlled clinical study, the clinical and immunomodulatory efficacy of miltefosine was i...


    pub_type: 杂志文章,随机对照试验


    authors: Dölle S,Hoser D,Rasche C,Loddenkemper C,Maurer M,Zuberbier T,Worm M

    更新日期:2010-09-01 00:00:00

  • IgE, IgG1, and IgG4 antibody responses to Blomia tropicalis in atopic patients.

    abstract:BACKGROUND:Allergens from house dust mites (HDMs), Dermatophagoides pteronyssinus and Blomia tropicalis are clinically relevant in atopic respiratory diseases in tropical countries. AIMS OF THE STUDY:To evaluate immunoglobulin (Ig)E, IgG1, and IgG4 antibody responses to B. tropicalis in Brazilian atopic patients. MET...


    pub_type: 杂志文章


    authors: Pereira EA,Silva DA,Cunha-Júnior JP,Almeida KC,Alves R,Sung SJ,Taketomi EA

    更新日期:2005-03-01 00:00:00

  • Omega 3 and 6 oils for primary prevention of allergic disease: systematic review and meta-analysis.

    abstract:BACKGROUND:There is conflicting evidence on the use of omega 3 and omega 6 supplementation for the prevention of allergic diseases. We conducted a systematic review evaluating the effectiveness of omega 3 and 6 oils for the primary prevention of sensitization and development of allergic disorders. METHODS:We searched ...


    pub_type: 杂志文章,meta分析,评审


    authors: Anandan C,Nurmatov U,Sheikh A

    更新日期:2009-06-01 00:00:00

  • Anaphylactic shock after skin test with phytohaemoagglutinin.

    abstract::A young woman (26 years old), with gastrointestinal candidosis of 8 months duration, was hospitalized in our department to study her cell immunity. Five minutes after an id injection of PHA (bactophytohaemoagglutinin M, Difco, 1 mg), mix monilieae, mix tricophyton and PPD Berna, she showed an extensive wheal and flare...


    pub_type: 杂志文章


    authors: Schiavino D,Nucera E,Murzilli F,Schinco G,Papa G,Fais G,Patriarca G

    更新日期:1992-04-01 00:00:00

  • Allergen cross-reactivity between Pityrosporum orbiculare and Candida albicans.

    abstract::Pityrosporum orbiculare and Candida albicans extracts were separated by SDS-PAGE, and IgE binding was detected by immunoblotting with 21 patient sera that were RAST positive to both yeasts. Cross-wise inhibition was performed of IgE binding of a serum pool containing IgE antibodies to both yeasts. The pool was mixed w...


    pub_type: 杂志文章


    authors: Huang X,Johansson SG,Zargari A,Nordvall SL

    更新日期:1995-08-01 00:00:00