Abstract:
:An affinity chromatography method for preparation of patient-related antigens from commercially available allergen extracts has been investigated. IgG1,2,4 from a patient previously hyposensitized with dog hair and dandruff allergen was bound to protein A-sepharose. Secondly, commercial allergen extract was applied to the immunosorbent, and patient-related antigens were selectively absorbed by the specific antibodies from the patient serum. Finally, immune complexes containing antigen and IgG were eluted. Alternatively, the antigens alone were purified by retaining the IgG on the column by means of a covalent reinforcement of the protein A-IgG-binding. Purified, patient-related antigens were investigated in crossed immunoelectrophoresis and identity to IgG- and IgE-binding antigens (as determined by the CRIE-technique) was suggested. The affinity purified IgG was unaltered with regard to protein A-binding, binding of antigen and affinity for monoclonal antibody to human IgG-subclasses. Further, it was demonstrated that the antigen-binding capacity of IgG in the immune complexes was intact as evidenced by strong affinity to antigens even at low pH. The antigens eluted together with IgG were predominantly found in immune complexes with a molecular weight of greater than 300 kdalton equivalent to 2 or more molecules of IgG. The possibility of employing a similar method with IgE instead of IgG for preparation of patient-related allergens instead of antigens, is discussed.
journal_name
Allergyjournal_title
Allergyauthors
Poulsen LK,Søndergaard I,Weeke Bdoi
10.1111/j.1398-9995.1988.tb00927.xsubject
Has Abstractpub_date
1988-11-01 00:00:00pages
552-64issue
8eissn
0105-4538issn
1398-9995journal_volume
43pub_type
杂志文章相关文献
ALLERGY文献大全abstract:BACKGROUND:The contribution of B-cell subsets and T-B cell interaction to the pathogenesis of allergic rhinitis (AR) and mechanisms of allergen immunotherapy (AIT) remain poorly understood. This study aimed to outline circulating B-cell signature, the underlying mechanism, and its association with clinical response to ...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.14288
更新日期:2020-10-01 00:00:00
abstract::The connective tissue disorders (also called collagen vascular diseases) represent an heterogeneous group of immunologically mediated inflammatory disorders with a large variety of affected organs besides the lungs. The respiratory system may be involved in all its components: airways, vessels, parenchyma, pleura, res...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1111/j.1398-9995.2005.00761.x
更新日期:2005-06-01 00:00:00
abstract::This study evaluated the risk factors for developing allergic reactions to alternative drugs such as acetaminophen and nimesulide in 367 patients intolerant of nonsteroidal anti-inflammatory drugs (NSAID) compared to 243 healthy controls. All subjects were given test doses (TD) of acetaminophen and nimesulide, and age...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1998.tb03995.x
更新日期:1998-09-01 00:00:00
abstract:BACKGROUND:The capacity of profilin to induce allergic symptoms in patients with respiratory allergy has been questioned. In this sense, the aim of this study was to investigate the correlation between profilin exposure and induction of symptoms in a prospective case-control study. METHODS:The concentration of profili...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.14140
更新日期:2020-04-01 00:00:00
abstract:BACKGROUND:The purpose of this study was to assess the occurrence of immunoglobulin E sensitization to common environmental allergens (atopy) and new allergic diseases among schoolchildren after starting school in a water-damaged school building. The staff and pupils of a Finnish elementary school with visible water da...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1034/j.1398-9995.2001.056002175.x
更新日期:2001-02-01 00:00:00
abstract::A group of nine patients with respiratory symptoms after nonspecific irritating stimuli, but without any IgE-mediated allergy or demonstrable bronchial obstruction, were referred to the asthma/allergy outpatient department for evaluation of suspected asthma. In order to find a provocation model and objectively assess ...
journal_title:Allergy
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1398-9995.1996.tb04644.x
更新日期:1996-06-01 00:00:00
abstract::A total of 105 "high-risk" infants born in 1988 were studied prospectively from birth to 18 months of age. The infants were recommended breastfeeding and/or hypoallergenic formula (Nutramigen or Profylac) combined with avoidance of solid foods during the first 6 months of life. All mothers had unrestricted diet. Avoid...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1992.tb00680.x
更新日期:1992-10-01 00:00:00
abstract::The eosinophilic granulocyte is commonly associated with allergic inflammation. Although blood eosinophilia frequently accompanies atopic dermatitis, accumulation of tissue eosinophils is not prominent. Recent studies have elucidated the structure, content, and activities of the eosinophil. In addition, immunofluoresc...
journal_title:Allergy
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract:BACKGROUND:The mechanisms driving glucocorticoid (GC) insensitivity in patients with severe asthma are still unknown. Recent evidence suggests the existence of GC-insensitive pathways in airway smooth muscle (ASM) caused by a defect in GC receptor (GRα) function. We examined whether other mechanisms could potentially e...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.13003
更新日期:2017-01-01 00:00:00
abstract:BACKGROUND:Subjective symptoms after food challenges are difficult to interpret and no standard is available. We discuss a strategy for how to interpret a diary. Furthermore, the interobserver reliability is evaluated. METHODS:Diaries for 32 patients with subjective symptoms were used. The diaries were re-evaluated wi...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2003.00437.x
更新日期:2004-03-01 00:00:00
abstract::There are studies demonstrating that skin-test sensitivity to penicillins can decrease over time and that allergic patients may lose sensitivity if the responsible compounds are avoided. With regard to subjects with IgE-mediated hypersensitivity to cephalosporins, however, such studies are lacking. We evaluated prospe...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.12390
更新日期:2014-06-01 00:00:00
abstract::The ability of common environmental allergens to stimulate IgE responses and thus to produce allergic diseases has tended to overshadow the fact that helminthic parasites are possibly the most potent inducers of this immunoglobulin that exists in nature. Although it has been well established that during these infectio...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1111/j.1398-9995.2006.00983.x
更新日期:2006-04-01 00:00:00
abstract:BACKGROUND:Staphylococcus aureus (S. aureus) is frequently detected in the skin of patients with atopic dermatitis (AD), and involved in the flare of AD. There are some evidence-specific strains of S. aureus affect the severity of AD. However, the mechanism of predominant colonization and the aggravation of dermatitis ...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.13622
更新日期:2019-03-01 00:00:00
abstract::Topical corticosteroids have proved to be effective in the treatment of allergic rhinitis. The symptomatology of allergic rhinitis is considered to be the result of the accumulation and activation of inflammatory cells and cytokine release and hence the efficacy of corticosteroids is associated with their anti-inflamm...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1111/j.1398-9995.1997.tb04819.x
更新日期:1997-01-01 00:00:00
abstract:BACKGROUND:As in vitro diagnosis of wasp venom sensitization by specific serum IgE has a sensitivity of only 60-80%, additional in vitro tests are desirable. Basophil activation is associated with the expression of CD63 and its measurement has been proposed as a novel in vitro test for immediate-type allergy. Furthermo...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2004.00624.x
更新日期:2004-10-01 00:00:00
abstract:BACKGROUND:In sublingual immunotherapy, optimal doses are a key factor for therapeutic outcomes. The aim of this study with tablets containing carbamylated monomeric house dust mite allergoids was to determine the most effective and safe dose. METHODS:In this double-blind, placebo-controlled dose-finding study, 131 pa...
journal_title:Allergy
pub_type: 杂志文章,多中心研究,随机对照试验
doi:10.1111/all.12913
更新日期:2017-01-01 00:00:00
abstract::We studied the effect of tea polyphenols on histamine release from rat basophilic leukemia (RBL-2H3) cells. Among tea polyphenols, (-)- epigallocatechin gallate (EGCG) most strongly and dose-dependently inhibited histamine release from cells stimulated with a calcium ionophore, A23187. (-)-Epigallocatechin (EGC) and (...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1997.tb02546.x
更新日期:1997-01-01 00:00:00
abstract::We describe a case of contact allergic sensitization to budesonide in a nasal spray used in the treatment of allergic rhinitis. No cross-sensitivity to other topical corticosteroids was found. ...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1992.tb02392.x
更新日期:1992-12-01 00:00:00
abstract::The phenotypes and receptors of the intraepithelial lymphocytes which are a major component of the mucosal immune system are of considerable interest. Intraepithelial lymphocytes, especially those of the intestines, are well documented. The nasal intraepithelial lymphocytes probably play a key role in the immune defen...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.1993.tb00693.x
更新日期:1993-02-01 00:00:00
abstract::Although genetic factors play a role in the etiology of atopic disease, the rapid increases in the prevalence of these diseases over the last few decades suggest that environmental, rather than genetic factors are the driving force behind the increasing prevalence. In modern societies, there is increased time spent in...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1111/all.14624
更新日期:2020-10-09 00:00:00
abstract:BACKGROUND:Dupilumab, a fully human monoclonal antibody that binds IL-4Rα and inhibits signaling of both IL-4 and IL-13, has shown efficacy across multiple diseases with underlying type 2 signatures and is approved for treatment of asthma, atopic dermatitis, and chronic sinusitis with nasal polyposis. We sought to prov...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.14151
更新日期:2020-05-01 00:00:00
abstract::Antigen-presenting cells (APCs) of the human gut are heterogeneous, including both macrophages, a variety of dendritic cells and B cells. They are found both in gut-associated lymphoid tissue and in the mucosal lamina propria, especially beneath the surface epithelium. APCs have diverse phenotypes and therefore probab...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1034/j.1398-9995.2001.00903.x
更新日期:2001-01-01 00:00:00
abstract:BACKGROUND:IgE is the central antibody isotype in TH2-biased immunity and allergic diseases. The structure of intact IgE and the impact of IgE-targeting molecules on IgE however remain elusive. In order to obtain insights into IgE biology and the clinical impact, we aimed for structure determination of IgE and the comp...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.14222
更新日期:2020-08-01 00:00:00
abstract:BACKGROUND:Hippo-Yes-associated protein (YAP) pathway plays an important role in epithelial cell proliferation and development. However, its possible role in chronic rhinosinusitis with nasal polyps (CRSwNP) remains unknown. We aim to investigate it on nasal epithelial proliferation and remodeling in CRSwNP. METHODS:T...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.13647
更新日期:2019-04-01 00:00:00
abstract:BACKGROUND:Chronic urticaria (CU) is a frequent skin disease characterized by relapsing appearance of pruritic hives. While clinical symptoms are due to the release of histamine by cutaneous mast cells, the underlying pathophysiology is still unknown. However, previous studies indicate that basophils might be of releva...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/all.13215
更新日期:2017-12-01 00:00:00
abstract::Environmental exposures in pregnancy and the early postnatal period affect early immune development. Early immune function and future allergy susceptibility appear to result from a combination of maternal phenotype, infant genotype, and environmental exposures in utero that affect early gene expression. Furthermore, e...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1111/j.1398-9995.2011.02620.x
更新日期:2011-07-01 00:00:00
abstract::The prick test is one of the most common medical methods for diagnosing allergies, and it has been carried out in a similar and laborious manner over many decades. In an attempt to standardize the reading of the test, many researchers have tried to automate the process of measuring the allergic reactions found by deve...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1111/all.12921
更新日期:2016-08-01 00:00:00
abstract:BACKGROUND:A favorable outcome of anaphylaxis depends on the rapidity of adequate initial management and epinephrine injection. However, few data on the management of anaphylactic reactions are available. The aim of this study was to investigate the management and awareness of anaphylaxis to foods by mean of an Interne...
journal_title:Allergy
pub_type: 杂志文章
doi:10.1034/j.1398-9995.2001.056006540.x
更新日期:2001-06-01 00:00:00
abstract::A shared approach to decision-making framework has been suggested for chronic disease management especially where multiple treatment options exist. Shared decision-making (SDM) requires that both physician and patients are actively engaged in the decision-making process, including information exchange; expressing trea...
journal_title:Allergy
pub_type: 杂志文章,评审
doi:10.1111/all.13090
更新日期:2017-05-01 00:00:00
abstract::Ten patients with bronchial asthma reacting with bronchoconstriction after exercise were studied to compare the effect of oral sodium cromoglycate against sodium cromoglycate inhalation and placebo. Only sodium cromoglycate inhalations protected against exercise-induced asthma. The effect of sodium cromoglycate seems ...
journal_title:Allergy
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1111/j.1398-9995.1980.tb01779.x
更新日期:1980-06-01 00:00:00