Preparation of patient-related allergens for hyposensitization. Qualitative aspects.


:An affinity chromatography method for preparation of patient-related antigens from commercially available allergen extracts has been investigated. IgG1,2,4 from a patient previously hyposensitized with dog hair and dandruff allergen was bound to protein A-sepharose. Secondly, commercial allergen extract was applied to the immunosorbent, and patient-related antigens were selectively absorbed by the specific antibodies from the patient serum. Finally, immune complexes containing antigen and IgG were eluted. Alternatively, the antigens alone were purified by retaining the IgG on the column by means of a covalent reinforcement of the protein A-IgG-binding. Purified, patient-related antigens were investigated in crossed immunoelectrophoresis and identity to IgG- and IgE-binding antigens (as determined by the CRIE-technique) was suggested. The affinity purified IgG was unaltered with regard to protein A-binding, binding of antigen and affinity for monoclonal antibody to human IgG-subclasses. Further, it was demonstrated that the antigen-binding capacity of IgG in the immune complexes was intact as evidenced by strong affinity to antigens even at low pH. The antigens eluted together with IgG were predominantly found in immune complexes with a molecular weight of greater than 300 kdalton equivalent to 2 or more molecules of IgG. The possibility of employing a similar method with IgE instead of IgG for preparation of patient-related allergens instead of antigens, is discussed.






Poulsen LK,Søndergaard I,Weeke B




Has Abstract


1988-11-01 00:00:00














  • Interleukin-33 in allergy.

    abstract::Interleukin-33 (IL-33) is a member of the IL-1 cytokine family, which includes IL-1 and IL-18, and is considered to be important for host defense against nematodes by inducing Th2 cytokine production via the IL-33 receptor. IL-33 receptor is a heterodimer of IL-1 receptor-like 1 (IL-1RL1; also called ST2, T1, Der4, an...


    pub_type: 杂志文章,评审


    authors: Ohno T,Morita H,Arae K,Matsumoto K,Nakae S

    更新日期:2012-10-01 00:00:00

  • Sub-lethal exposure of cockroaches to boric acid pesticide contributes to increased Bla g 2 excretion.

    abstract:BACKGROUND:Several epidemiology studies have found an increase in the major cockroach allergen Bla g 2 with reported pesticide use. AIMS OF THE STUDY:Our aim was to investigate the effect on the excretion of Bla g 1 and Bla g 2 allergens by cockroaches exposed to sub-lethal doses of the pesticides, boric acid and hydr...


    pub_type: 杂志文章


    authors: Zhang YC,Perzanowski MS,Chew GL

    更新日期:2005-07-01 00:00:00

  • Proteome analysis identifies L1CAM/CD171 and DPP4/CD26 as novel markers of human skin mast cells.

    abstract:BACKGROUND:The function of skin mast cells has been well documented in IgE-mediated allergic reactions, whereas other mast cell functions are poorly defined. This study aimed at identifying novel mast cell proteins by proteome analysis of primary human skin mast cells. METHODS:The proteome of skin mast cells was compa...


    pub_type: 杂志文章


    authors: Gschwandtner M,Paulitschke V,Mildner M,Brunner PM,Hacker S,Eisenwort G,Sperr WR,Valent P,Gerner C,Tschachler E

    更新日期:2017-01-01 00:00:00

  • Alpine climate treatment of atopic dermatitis: a systematic review.

    abstract::Climate therapy has been used for decades in the treatment of atopic dermatitis (AD), but evidence of its effectiveness has not yet been assessed systematically. A systematic literature search in Medline, Embase, and the Cochrane library was performed to identify all original studies concerning alpine climate treatmen...


    pub_type: 杂志文章,meta分析,评审


    authors: Fieten KB,Weststrate AC,van Zuuren EJ,Bruijnzeel-Koomen CA,Pasmans SG

    更新日期:2015-01-01 00:00:00

  • Double-blind comparison of the protective effect of sodium cromoglycate and ketotifen on exercise-induced asthma in adults.

    abstract::In a double-blind double-placebo study 10 asthmatics exercised on a treadmill after treatment for 1 week with placebo, or ketotifen 1 mg twice daily, or SCG 10 mg four times daily. The post-exercise percentage fall in FEV1 after SCG but not after ketotifen was significantly smaller than after placebo. A 1-week treatme...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Tan WC,Lim TK

    更新日期:1987-05-01 00:00:00

  • Kiwifruit Act d 11 is the first member of the ripening-related protein family identified as an allergen.

    abstract:BACKGROUND:Kiwifruit is an important cause of food allergy. A high amount of a protein with a molecular mass compatible with that of Bet v 1 was observed in the kiwifruit extract. OBJECTIVE:To identify and characterize kirola, the 17-kDa protein of green kiwifruit (Act d 11). METHODS:Act d 11 was purified from green ...


    pub_type: 杂志文章


    authors: D'Avino R,Bernardi ML,Wallner M,Palazzo P,Camardella L,Tuppo L,Alessandri C,Breiteneder H,Ferreira F,Ciardiello MA,Mari A

    更新日期:2011-07-01 00:00:00

  • Evaluating the efficacy of breastfeeding guidelines on long-term outcomes for allergic disease.

    abstract:BACKGROUND:WHO guidelines advocate breastfeeding for 6 months, and EAACI guideline recommends exclusive breastfeeding for 4-6 months. However, evidence for breastfeeding to prevent asthma and allergic disease is conflicting. We examined whether following recommended breastfeeding guidelines alters the long-term risks o...


    pub_type: 杂志文章


    authors: Bion V,Lockett GA,Soto-Ramírez N,Zhang H,Venter C,Karmaus W,Holloway JW,Arshad SH

    更新日期:2016-05-01 00:00:00

  • Pseudomonas aeruginosa allergy in cystic fibrosis. Involvement of histamine release in the pathogenesis of lung tissue damage.

    abstract::Basophil histamine release by P. aeruginosa standard antigen was examined in cystic fibrosis patients chronically infected with mucoid P. aeruginosa (CF +P) and with pronounced antibody response against these bacteria, and in patients without P. aeruginosa infection (CF +P). All the patients showed eosinophil counts a...


    pub_type: 杂志文章


    authors: Skov PS,Norn S,Schiøtz PO,Permin H,Høiby N

    更新日期:1980-01-01 00:00:00

  • Milk whey-specific immune complexes in allergic and non-allergic subjects.

    abstract::We developed an antigen- and isotype-specific ELISA for the rapid detection of native serum immune complexes (IC). It is a sandwich assay, in which a solid phase antigen-specific capture antibody selectively binds the antigen-specific IC via the antigen. The isotype of the bound IC is then identified using an enzyme-l...


    pub_type: 杂志文章


    authors: Bell SJ,Potter PC

    更新日期:1988-10-01 00:00:00

  • Evaluation of cytokine mRNA in induced sputum from patients with allergic rhinitis: relationship to airway hyperresponsiveness.

    abstract:BACKGROUND:Although airway hyperresponsiveness (AHR) is a characteristic feature of asthma, it is also frequently present in allergic rhinitis (AR). However, the pathogenesis of AHR is unclear and the roles of cytokines in the airway have not been well established in AR. We sought to compare cytokine mRNA levels in the...


    pub_type: 杂志文章


    authors: Sohn SW,Lee HS,Park HW,Chang YS,Kim YK,Cho SH,Kim YY,Min KU

    更新日期:2008-03-01 00:00:00

  • Surfactant protein levels in bronchoalveolar lavage after segmental allergen challenge in patients with asthma.

    abstract:BACKGROUND:Allergic asthma is associated with airway inflammation and dysfunction of pulmonary surfactant. Because surfactant proteins (SP) account for immunomodulatory functions as well as biophysical functions, we hypothesized that the allergic response in asthma might be accompanied by a dysregulation of SPs. METHO...


    pub_type: 杂志文章


    authors: Erpenbeck VJ,Schmidt R,Günther A,Krug N,Hohlfeld JM

    更新日期:2006-05-01 00:00:00

  • Safety and reproducibility of nasal allergen challenge.

    abstract:BACKGROUND:The nasal allergen challenge (NAC) is a useful tool for the diagnosis of allergic rhinitis (AR) and local allergic rhinitis (LAR) and might serve to design and monitor allergen immunotherapy. Nevertheless, data about its safety and reproducibility are scarce. OBJECTIVE:To investigate the safety and reproduc...


    pub_type: 杂志文章


    authors: Eguiluz-Gracia I,Testera-Montes A,González M,Pérez-Sánchez N,Ariza A,Salas M,Moreno-Aguilar C,Campo P,Torres MJ,Rondon C

    更新日期:2019-06-01 00:00:00

  • Advances and novel developments in environmental influences on the development of atopic diseases.

    abstract::Although genetic factors play a role in the etiology of atopic disease, the rapid increases in the prevalence of these diseases over the last few decades suggest that environmental, rather than genetic factors are the driving force behind the increasing prevalence. In modern societies, there is increased time spent in...


    pub_type: 杂志文章,评审


    authors: Alkotob SS,Cannedy C,Harter K,Movassagh H,Paudel B,Prunicki M,Sampath V,Schikowski T,Smith E,Zhao Q,Traidl-Hoffmann C,Nadeau KC

    更新日期:2020-10-09 00:00:00

  • Pharyngeal edema caused by occupational exposure to cellulase enzyme.

    abstract::Enzymes can cause allergic asthma and rhinitis. Although patients sometimes complain of symptoms in their throat, we have not been able to find any published report of pharyngeal edema caused by exposure to enzymes. We present the case of a maintenance foreman who worked in a factory producing enzymes, and who had pha...


    pub_type: 杂志文章


    authors: Hytönen M,Vanhanen M,Keskinen H,Tuomi T,Tupasela O,Nordman H

    更新日期:1994-10-01 00:00:00

  • Immunoglobulin class and subclass distribution of dextran-reactive antibodies in human reactors and non reactors to clinical dextran.

    abstract::The red cell-linked antigen-antiglobulin reaction (RCLAAR) with stearoyldextran-coated erythrocytes was used to characterize the immunoglobulin (Ig) classes and IgG subclasses of dextran reactive antibodies (DRA) in 27 dextran reactors (DR) and 96 on reactors (DNR). High titres of dextran reactive IgG were regularly f...


    pub_type: 杂志文章


    authors: Kraft D,Hedin H,Richter W,Scheiner O,Rumpold H,Devey ME

    更新日期:1982-10-01 00:00:00

  • Nasal inhalation of budesonide from a spacer in children with perennial rhinitis and asthma.

    abstract::The standard treatment of allergic rhinitis and asthma consists of topical corticosteroids administered intranasally and inhaled through the mouth. Although this therapy is highly effective, and side-effects are few and mild, it may be possible further to improve the therapeutic index and patient compliance with the t...


    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Pedersen W,Hjuler I,Bisgaard H,Mygind N

    更新日期:1998-04-01 00:00:00

  • The role of drug, dose, and the tolerance/intolerance of new drugs in multiple drug hypersensitivity syndrome.

    abstract:BACKGROUND:Multiple drug hypersensitivity syndrome (MDH) is used to describe persons with a drug hypersensitivity reaction (DHR) to at least two chemically unrelated drugs, confirmed by skin test or in vitro assay. METHODS:Medical records of 25 patients with MDH, tested and confirmed at our allergy division, were retr...


    pub_type: 杂志文章


    authors: Jörg L,Yerly D,Helbling A,Pichler W

    更新日期:2020-05-01 00:00:00

  • The basophil activation test in wasp venom allergy: sensitivity, specificity and monitoring specific immunotherapy.

    abstract:BACKGROUND:As in vitro diagnosis of wasp venom sensitization by specific serum IgE has a sensitivity of only 60-80%, additional in vitro tests are desirable. Basophil activation is associated with the expression of CD63 and its measurement has been proposed as a novel in vitro test for immediate-type allergy. Furthermo...


    pub_type: 杂志文章


    authors: Erdmann SM,Sachs B,Kwiecien R,Moll-Slodowy S,Sauer I,Merk HF

    更新日期:2004-10-01 00:00:00

  • Fungal propagules in house dust. I. Comparison of analytic methods and their value as estimators of potential exposure.

    abstract::The presence of viable mold propagules in house dust was investigated by 10 different analytic methods, in order to determine to what extent different results are obtained when different analytic methods are used. Moreover, the value of this measurement as an estimator of the potential exposure to fungi in epidemiolog...


    pub_type: 杂志文章


    authors: Verhoeff AP,van Reenen-Hoekstra ES,Samson RA,Brunekreef B,van Wijnen JH

    更新日期:1994-08-01 00:00:00

  • A comparative study of the characteristics of intraepithelial and lamina propria lymphocytes of the human nasal mucosa.

    abstract::The phenotypes and receptors of the intraepithelial lymphocytes which are a major component of the mucosal immune system are of considerable interest. Intraepithelial lymphocytes, especially those of the intestines, are well documented. The nasal intraepithelial lymphocytes probably play a key role in the immune defen...


    pub_type: 杂志文章


    authors: Pawankar R,Okuda M

    更新日期:1993-02-01 00:00:00

  • Role of imbalance of eicosanoid pathways and staphylococcal superantigens in chronic rhinosinusitis.

    abstract::Chronic rhinosinusitis (CRS) is a multifactorial disease of the upper airways with a high prevalence (approximately 11%) in the general population. Different immune and inflammatory mechanisms are involved in its pathogenesis. Alterations in the arachidonic acid pathway (leading to an imbalanced production of eicosano...


    pub_type: 杂志文章,评审


    authors: Pezato R,Świerczyńska-Krępa M,Niżankowska-Mogilnicka E,Derycke L,Bachert C,Pérez-Novo CA

    更新日期:2012-11-01 00:00:00

  • Effect of a bronchial provocation test with house-dust mite on blood eosinophilia, eosinophil cationic protein, soluble interleukin-2 receptor, and interleukin-6 in asthmatic children.

    abstract::Eighteen children with perennial asthma and allergy to house-dust mite (HDM) underwent a bronchial challenge with HDM. Before and 24 h after the test, a venous blood sample was taken to determine levels of eosinophils, eosinophil cationic protein (ECP), soluble interleukin-2 receptor (IL-2R), and interleukin-6 (IL-6)....


    pub_type: 杂志文章


    authors: Van Bever HP,Moens MM,Bridts CH,De Clerck LS,Mertens AV,Bosmans E,Stevens WJ

    更新日期:1993-08-01 00:00:00

  • Development, validity and reliability of the food allergy independent measure (FAIM).

    abstract:BACKGROUND:The Food Allergy Quality of Life Questionnaire-Child Form, -Teenager Form and -Adult Form (FAQLQ-CF, -TF and -AF) have recently been developed. To measure construct validity in the FAQLQs, a suitable independent measure was needed with which FAQLQ scores could be correlated. However, in food allergy, no appr...


    pub_type: 杂志文章


    authors: van der Velde JL,Flokstra-de Blok BM,Vlieg-Boerstra BJ,Oude Elberink JN,DunnGalvin A,Hourihane JO,Duiverman EJ,Dubois AE

    更新日期:2010-05-01 00:00:00

  • An approach to the understanding of the nasal early-phase reaction induced by nasal allergen challenge.

    abstract::Quantitative determinations of the inflammatory mediators in nasal secretions were performed and correlated with the objective nasal symptoms within 1 h after nasal allergen challenge (NAC). Twenty-six patients with seasonal allergic rhinitis were enrolled outside the pollen season. All measurements were performed bef...


    pub_type: 杂志文章


    authors: Wang D,Smitz J,Waterschoot S,Clement P

    更新日期:1997-02-01 00:00:00

  • Hypersensitivity to cefuroxime with good tolerance to other betalactams.

    abstract::A 60-year-old man developed an anaphylactic reaction immediately after administration of intramuscular sodium cefuroxime. By means of oral controlled challenge, the reaction was reproduced with a smaller dose of cefuroxime axetil. Cross-reactivity with other betalactam antibiotics was ruled out by in vivo tests. This ...


    pub_type: 杂志文章


    authors: Marcos Bravo C,Luna Ortiz I,González Vázquez R

    更新日期:1995-04-01 00:00:00

  • New European Academy of Allergy and Clinical Immunology definition on pollen season mirrors symptom load for grass and birch pollen-induced allergic rhinitis.

    abstract:BACKGROUND:The use of allergen immunotherapy (AIT) for allergic rhinitis and its clinical efficacy in clinical trials depends on the effective determination of pollen allergen exposure time periods. We evaluate pollen data from Germany to examine the new definitions on pollen season and peak pollen period start and end...


    pub_type: 杂志文章


    authors: Karatzas K,Katsifarakis N,Riga M,Werchan B,Werchan M,Berger U,Pfaar O,Bergmann KC

    更新日期:2018-09-01 00:00:00

  • The epidemiology of anaphylaxis in Europe: a systematic review.

    abstract:BACKGROUND:Anaphylaxis is an acute, potentially fatal, multi-organ system, allergic reaction caused by the release of chemical mediators from mast cells and basophils. Uncertainty exists around epidemiological measures of incidence and prevalence, risk factors, risk of recurrence, and death due to anaphylaxis. This sys...


    pub_type: 杂志文章,评审


    authors: Panesar SS,Javad S,de Silva D,Nwaru BI,Hickstein L,Muraro A,Roberts G,Worm M,Bilò MB,Cardona V,Dubois AE,Dunn Galvin A,Eigenmann P,Fernandez-Rivas M,Halken S,Lack G,Niggemann B,Santos AF,Vlieg-Boerstra BJ,Zolkipli Z

    更新日期:2013-11-01 00:00:00

  • Allergen-induced release of sulphidopeptide leukotrienes (SRS-A) and LTB4 in allergic rhinitis.

    abstract::Leukotrienes are a recently discovered group of arachidonic acid-derived lipid mediators. Using radioimmunoassay and high pressure liquid chromatography (HPLC), we have identified the SRS-A sulphidopeptide leukotrienes (LTC4, LTD4 and LTE4) in nasal washings from patients with allergic rhinitis who underwent nasal cha...


    pub_type: 杂志文章


    authors: Shaw RJ,Fitzharris P,Cromwell O,Wardlaw AJ,Kay AB

    更新日期:1985-01-01 00:00:00

  • Allergy to jackfruit: a novel example of Bet v 1-related food allergy.

    abstract:BACKGROUND:Jackfruit allergy has been reported just once. It is unknown whether this food allergy is caused by direct sensitization or cross-sensitization to pollen allergens. OBJECTIVE:Establish whether jackfruit allergy is linked to birchpollen allergy. METHODS:Two jackfruit allergic patients and five patients with...


    pub_type: 杂志文章


    authors: Bolhaar ST,Ree R,Bruijnzeel-Koomen CA,Knulst AC,Zuidmeer L

    更新日期:2004-11-01 00:00:00

  • Can food allergy be cured? What are the future prospects?

    abstract::Food allergies have become a significant heath burden as prevalence continues to rise, affecting 6%-13% of the global population. In the absence of drugs approved by regulatory agencies, the current standard of care remains avoidance of allergenic foods and management of acute allergic reactions with antihistamines an...


    pub_type: 杂志文章,评审


    authors: Sampath V,Sindher SB,Alvarez Pinzon AM,Nadeau KC

    更新日期:2020-06-01 00:00:00