Abstract：
:The effects of cytochalasin D on basal and adrenocorticotropin (ACTH)-stimulated steroid production as well as stress fiber organization by bovine adrenocortical (BAC) cells grown in monolayer culture have been investigated. Corticosteroid and pregnenolone release was determined by use of radioimmunoassay. The addition of ACTH (1 nM) produced a 30-fold increase in steroid release (principally corticosterone) in a 2 h period. Cytochalasin D (1-10 microM) had no effect on ACTH-stimulated release of corticosteroids. Basal steroid release was elevated by cytochalasin D with lower concentrations being more effective. In addition, hormonal stimulation of pregnenolone formation, the initial step in cholesterol metabolism leading to corticosteroid production, was also unaffected by cytochalasin D (1-50 microM) addition. Observation of stress fibers by fluorescence microscopy using the probe NDB - phalladin revealed that cytochalasin D (10 and 50 microM) caused an aggregation of actin-containing filaments into stellate foci within the cytoplasm. This was confirmed by electron microscopic examination of the cells. ACTH, however, had no observable effect on stress fiber organization or cell morphology. These results differ from the inhibitory effect of cytochalasin on steroid production observed using Y-1 adrenal tumor cells and rat adrenal cells in primary culture. BAC cells in contrast to Y-1 adrenal tumor cells differ in non-esterified cholesterol content and their morphological response to ACTH treatment. We suggest that these differences may influence the lack of inhibition of cytochalasin D on ACTH-stimulated steroid release.
journal_name
Mol Cell Endocrinoljournal_title
Molecular and cellular endocrinologyauthors
Rainey WE,Shay JW,Mason JIdoi
10.1016/0303-7207(84)90016-9subject
Has Abstractpub_date
1984-05-01 00:00:00pages
189-97issue
2-3eissn
0303-7207issn
1872-8057journal_volume
35pub_type
杂志文章abstract：:Glucocorticoid hormones suppress circulating concentrations of thyrotrophin (TSH), but their effect on synthesis of TSH in the pituitary gland is unclear. We have examined the influence of the glucocorticoid dexamethasone on serum TSH, pituitary TSH content and TSH beta- and alpha-subunit mRNA concentrations in pituit...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(89)90064-6
更新日期：1989-06-01 00:00:00
abstract：:If human papillomavirus (HPV) is necessary for the development of (pre)neoplastic lesions of the uterine cervix, it is not sufficient. Among the cofactors involved in the malignant transformation of cells infected by HPV, sex hormones may facilitate the cervical carcinogenesis by different mechanisms, including the in...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2006.10.014
更新日期：2007-01-29 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2017.07.024
更新日期：2018-01-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(98)00110-5
更新日期：1998-07-25 00:00:00
abstract：:Fibroblasts from three patients with vitamin D-dependency rickets type II were used to study mutations in the 1,25-dihydroxyvitamin D3 receptor responsible for this hereditary disease. Normal human fibroblasts contain 43 +/- 13 fmol receptor/mg protein as determined by immunoradiometric assay and 22 +/- 3 fmol/mg by l...
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pub_type： 杂志文章
doi：10.1016/0303-7207(93)90152-a
更新日期：1993-01-01 00:00:00
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journal_title：Molecular and cellular endocrinology
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doi：10.1016/j.mce.2013.01.014
更新日期：2013-05-22 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2010.12.008
更新日期：2011-02-10 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(91)90224-g
更新日期：1991-02-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(89)90166-4
更新日期：1989-08-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.mce.2012.10.030
更新日期：2013-01-30 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2005.06.004
更新日期：2005-08-30 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2013.12.009
更新日期：2014-03-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2018.01.007
更新日期：2018-09-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2014.11.009
更新日期：2015-01-15 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2016.08.015
更新日期：2017-02-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 临床试验,杂志文章
doi：10.1016/j.mce.2006.10.010
更新日期：2007-01-29 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2020.110888
更新日期：2021-01-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/s0303-7207(00)00427-5
更新日期：2001-01-22 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2013.03.009
更新日期：2014-01-25 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2013.05.012
更新日期：2013-11-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2016.09.024
更新日期：2017-02-05 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2005.11.017
更新日期：2006-02-26 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2012.11.019
更新日期：2013-05-22 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(76)90090-3
更新日期：1976-08-01 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/j.mce.2008.03.006
更新日期：2008-06-11 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(96)03774-4
更新日期：1996-04-19 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(94)90242-9
更新日期：1994-05-01 00:00:00
abstract：:Interleukin-6 (IL-6) is a multifunctional cytokine involved in autoimmune thyroid diseases such as Hashimoto's thyroiditis and Graves' disease. IL-6 is produced by infiltrating immune cells and by thyrocytes. In the latter cell type, secretion of IL-6 is stimulated notably by interleukin-1 (IL-1), thyroid-stimulating ...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/j.mce.2007.12.017
更新日期：2008-03-12 00:00:00
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journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章,评审
doi：10.1016/s0303-7207(99)00051-9
更新日期：1999-05-25 00:00:00
abstract：:The ovarian granulosa cell has previously been shown to be a site of insulin-like growth factor (IGF) I production, reception, and action. It is the objective of this study to characterize in greater detail the soluble IGF binding activity released by this cell type. To this end, use was made of granulosa cells from i...
journal_title：Molecular and cellular endocrinology
pub_type： 杂志文章
doi：10.1016/0303-7207(90)90222-t
更新日期：1990-12-21 00:00:00
