Abstract:
:2-3H-Labelled 4-acetoxyaminoquinoline-1-oxide (Ac-4 HAQO), the ultimate carcinogen model of 4-nitroquinoline-1-oxide, was reacted in vitro with native and denatured DNA. We found that Ac-4 HAQO is 2- to 3-fold more reactive than diAc-4 HAQO, another ultimate carcinogen model of 4 NQO which was previously studied [Galiègue et al. (1980) Biochim. Biophys. Acta, 609, 383-391]. Ac-4 HAQO-modified DNA is thermally destabilized: when 1% of the bases of DNA were modified by Ac-4 HAQO, its melting temperature decreased 1.2 degrees C. Enzymatic degradation of Ac-4 HAQO-modified native and denatured DNA's to nucleosides was performed. The hydrolysates were analyzed, first with a simple chromatographic system, and then by h.p.l.c. The compounds recovered from the modified polymers were characterized by h.p.l.c. and a variation in their respective amounts as a function of the secondary structure of DNA was observed. Especially, the N-(deoxyguanosin-(C8-yl)-4-aminoquinoline-1-oxide, the so called dG III adduct, was recovered from DNA, and its amount was evaluated to be approximately 3.5-fold greater in the case of denatured DNA than in the case of native DNA.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Galiègue-Zouitina S,Bailleul B,Loucheux-Lefebvre MHdoi
10.1093/carcin/4.3.249subject
Has Abstractpub_date
1983-01-01 00:00:00pages
249-54issue
3eissn
0143-3334issn
1460-2180journal_volume
4pub_type
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