Regulation of gap-junctional intercellular communication in transformation-sensitive and transformation-resistant BALB/c 3T3 cell variants.


:BALB/c 3T3 A31-1-13 cells are highly susceptible to chemical-induced cell transformation and their gap-junctional intercellular communication (GJIC) is decreased when these cells become confluent. On the other hand, A31-1-1 cells do not show such a change in GJIC and are more resistant to chemical induction of transformation. In order to see which phenotypes are dominant, cells of these two types were hybridized and the hybrids were analyzed for their phenotypes for GJIC and induction of cell transformation. These two cell lines were tagged with neor gene or hygromycin-resistance gene respectively, and their hybrids were selected in medium containing G418 and hygromycin. Six independently isolated clones were characterized and all showed a loss of intercellular communication at confluence, suggesting that the phenotype of transformation-sensitive cell line A31-1-13 was dominant. However, when these hybrid cells were exposed to 3-methylcholanthrene, no transformed foci were produced, suggesting that the transformation-resistant phenotype is dominant. These results suggest that the regulation of GJIC and the susceptibility to chemical induction of transformation are genetically separate traits, and indirectly suggest that the loss of GJIC alone cannot explain the high susceptibility of A31-1-13 cells to induction of transformation.






Katoh F,Yamasaki H





Has Abstract


1991-10-01 00:00:00












  • Heterocyclic aromatic amines induce DNA strand breaks and cell transformation.

    abstract::Heterocyclic aromatic amines (HAAs), formed during the cooking of foods, are known to induce tumours in rodent bioassays and may thus contribute to human cancer risk. We tested six HAAs in a morphological transformation assay and in three in vitro genotoxicity assays. The morphological transforming abilities of HAAs w...


    pub_type: 杂志文章


    authors: Pfau W,Martin FL,Cole KJ,Venitt S,Phillips DH,Grover PL,Marquardt H

    更新日期:1999-04-01 00:00:00

  • Diacylglycerol is an effector of the clonal expansion of cells containing activated Ha-ras genes.

    abstract::Diacylglycerols (DAG) are lipid second messengers which are generated during phospholipase-catalyzed hydrolysis of phospholipids. The model DAG, sn-1,2-didecanoylglycerol (DIC10), is an effective topical tumor promoter in 7,12-dimethylbenz[a]anthracene (DMBA)-initiated mouse skin. We now report that 11/12 of DMBA-init...


    pub_type: 杂志文章


    authors: Mills KJ,Reynolds SH,Smart RC

    更新日期:1993-12-01 00:00:00

  • A CCL2/ROS autoregulation loop is critical for cancer-associated fibroblasts-enhanced tumor growth of oral squamous cell carcinoma.

    abstract::Cancer-associated fibroblasts (CAFs) have been described to play critical roles in initiation, progression and metastasis of various cancers. However, the involvement of CAFs in oral cancer (OC) has not been well addressed. In this study, we demonstrate that CAFs, when cocultured with OC cells (OCCs), produce high lev...


    pub_type: 杂志文章


    authors: Li X,Xu Q,Wu Y,Li J,Tang D,Han L,Fan Q

    更新日期:2014-06-01 00:00:00

  • Prevention of 1,2-dimethylhydrazine-induced colon tumorigenesis by HMG-CoA reductase inhibitors, pravastatin and simvastatin, in ICR mice.

    abstract::3-Hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase inhibitors, pravastatin (Pr) and simvastatin (Si), suppressed 1,2-dimethylhydrazine (DMH)-induced colon cancer development in female ICR mice. All mice received an i.p. injection of 10 mg DMH/kg body wt once weekly for 15 weeks. Pr was administered at 0.01, 0.0...


    pub_type: 杂志文章


    authors: Narisawa T,Fukaura Y,Terada K,Umezawa A,Tanida N,Yazawa K,Ishikawa C

    更新日期:1994-09-01 00:00:00

  • Tissue protein turnover during liver carcinogenesis.

    abstract::Overall rates of tissue protein degradation in vivo during chemical hepatocarcinogenesis were estimated by a double-isotope method as well as from the accumulation of peptide intermediates in protein degradation induced by bestatin. Several parameters estimating rates of cell proliferation and cell loss have been meas...


    pub_type: 杂志文章


    authors: Canuto RA,Tessitore L,Muzio G,Autelli R,Baccino FM

    更新日期:1993-12-01 00:00:00

  • G to A transitions and G to T transversions in codon 12 of the Ki-ras oncogene isolated from mouse lung tumors induced by 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and related DNA methylating and pyridyloxobutylating agents.

    abstract::Lung tumors were induced in A/J mice by the tobacco-specific nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and the related compounds acetoxymethylmethylnitrosamine (AMMN) and 4-acetoxymethylnitrosamino)-1-(3-pyridyl)-1-butanone (NNKOAc). NNK both methylates and pyridyloxobutylates DNA while AMMN and...


    pub_type: 杂志文章


    authors: Ronai ZA,Gradia S,Peterson LA,Hecht SS

    更新日期:1993-11-01 00:00:00

  • Enhanced repair of pyrimidine dimers in coding and non-coding genomic sequences in CHO cells expressing a prokaryotic DNA repair gene.

    abstract::We have previously demonstrated that the active dihydrofolate reductase (DHFR) gene is efficiently repaired in Chinese hamster ovary (CHO) cells which remove only a small fraction of u.v.-induced pyrimidine dimers from the overall genome. Preferential DNA repair of essential genes may explain why the u.v. resistance o...


    pub_type: 杂志文章


    authors: Bohr VA,Hanawalt PC

    更新日期:1987-09-01 00:00:00

  • A three-dimensional molecular template for substrates of human cytochrome P450 involved in debrisoquine 4-hydroxylation.

    abstract::A three-dimensional molecular template has been generated for substrates of human debrisoquine 4-hydroxylase cytochrome P450 (CYP2D6). This template defines the stereochemical requirements for CYP2D6 substrates in terms of the volume occupied and positions of key atoms. The modelling was based on the X-ray crystallogr...


    pub_type: 杂志文章


    authors: Islam SA,Wolf CR,Lennard MS,Sternberg MJ

    更新日期:1991-12-01 00:00:00

  • Metabolic activation of benzo[a]pyrene by a human hepatoma cell line.

    abstract::The liver-derived human cell line, Hep G2, has high benzo[a]pyrene-metabolizing activity and converts benzo[a]pyrene to intermediates that are mutagenic and that bind to DNA. This cell line will be useful for studying metabolic activation of polycyclic aromatic hydrocarbons and other xenobiotics by human tissue and as...


    pub_type: 杂志文章


    authors: Diamond L,Kruszewski F,Aden DP,Knowles BB,Baird WM

    更新日期:1980-01-01 00:00:00

  • Sodium arsenite administration via drinking water increases genome-wide and Ha-ras DNA hypomethylation in methyl-deficient C57BL/6J mice.

    abstract::Arsenic is an established human carcinogen. Deficiencies in available animal models have inhibited a detailed analysis of the mechanism of arsenic induced cancer. This study sought to determine the role of a methyl-deficient diet in combination with sodium arsenite on the genomic methylation status and Ha-ras methylat...


    pub_type: 杂志文章


    authors: Okoji RS,Yu RC,Maronpot RR,Froines JR

    更新日期:2002-05-01 00:00:00

  • The coordinate regulation of DNA synthesis and suppression of apoptosis is differentially regulated by the liver growth agents, phenobarbital and methylclofenapate.

    abstract::The coordinate regulation of DNA synthesis and suppression of apoptosis was investigated in a rat hepatocyte cell culture system which supports high level induction of DNA synthesis by the peroxisome proliferator, methylclofenapate (MCP) (Plant, N.J. et al., 1998, Carcinogenesis, 19, 925-931). The peroxisome prolifera...


    pub_type: 杂志文章


    authors: Plant NJ,Horley NJ,Dickins M,Hasmall S,Elcombe CR,Bell DR

    更新日期:1998-09-01 00:00:00

  • Molecular analysis of mutations induced by 2-chloroacetaldehyde, the ultimate carcinogenic form of vinyl chloride, in human cells using shuttle vectors.

    abstract::Vinyl chloride (VC) is a carcinogen associated with human and animal cancers. The ultimate carcinogenic form of VC, 2-chloroacetaldehyde (CAA), has been suspected to be mutagenic and we confirmed the mutagenicity of CAA using a modified shuttle vector plasmid. Base sequence analyses of 109 mutant plasmids with mutatio...


    pub_type: 杂志文章


    authors: Matsuda T,Yagi T,Kawanishi M,Matsui S,Takebe H

    更新日期:1995-10-01 00:00:00

  • Polyphenol-rich sweet potato greens extract inhibits proliferation and induces apoptosis in prostate cancer cells in vitro and in vivo.

    abstract::Sweet potato (Ipomoea batatas) leaves or greens, extensively consumed as a vegetable in Africa and Asia, are an excellent source of dietary polyphenols such as anthocyanins and phenolic acids. Here, we show that sweet potato greens extract (SPGE) has the maximum polyphenol content compared with several commercial vege...


    pub_type: 杂志文章


    authors: Karna P,Gundala SR,Gupta MV,Shamsi SA,Pace RD,Yates C,Narayan S,Aneja R

    更新日期:2011-12-01 00:00:00

  • 3-Methylcholanthrene-inhibition of hepatocarcinogenesis in the rat due to 3'-methyl-4-dimethylaminoazobenzene or 2-acetylamino-fluorene: a comparative study.

    abstract::Male Leeds strain rats were given a diet containing either 0.06% 3'-methyl-4-dimethylaminoazobenzene (3'-MeDAB) or 0.05% 2-acetylaminofluorene (2-AAF), alone or together with 0.0067% 3-methylcholanthrene (MeCh). MeCh both reduced the incidence of hepatic neoplasms induced by 3'-MeDAB and delayed their development. Wit...


    pub_type: 杂志文章


    authors: Flaks A,Flaks B

    更新日期:1982-01-01 00:00:00

  • Dehydroepiandrosterone and 16 alpha-bromo-epiandrosterone: inhibitors of Epstein-Barr virus-induced transformation of human lymphocytes.

    abstract::Dehydroepiandrosterone (DHEA), a major adrenal secretory product in men and women, is a potent inhibitor of mammalian glucose-6-phosphate dehydrogenase (G6PDH). Long-term treatment with this steroid has previously been found to suppress spontaneous breast cancer development in C3H mice. DHEA is now shown to inhibit Ep...


    pub_type: 杂志文章


    authors: Henderson E,Schwartz A,Pashko L,Abou-Gharbia M,Swern D

    更新日期:1981-01-01 00:00:00

  • N-acetyltransferase-dependent activation of 2-hydroxyamino-1-methyl-6-phenylimidazo[4,5-b]pyridine: formation of 2-amino-1-methyl-6-(5-hydroxy)phenylimidazo [4,5-b]pyridine, a possible biomarker for the reactive dose of 2-amino-1-methyl-6-phenylimidazo[4,

    abstract::2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) is a mutagenic and carcinogenic heterocyclic amine formed during ordinary cooking. PhIP is metabolically activated to the ultimate mutagenic metabolite by CYP P450-mediated N-hydroxylation followed by phase II esterification. Incubation of N-hydroxy-PhIP (N-OH-PhI...


    pub_type: 杂志文章


    authors: Frandsen H,Alexander J

    更新日期:2000-06-01 00:00:00

  • Membrane-type matrix metalloproteinases mediate curcumin-induced cell migration in non-tumorigenic colon epithelial cells differing in Apc genotype.

    abstract::Colonic epithelial cell migration is required for normal differentiated cell function. This migratory phenotype is dependent upon wild-type adenomatous polyposis coli (Apc) expression. Non-tumorigenic murine colon epithelial cell lines with distinct Apc genotypes, i.e. young adult mouse colon (YAMC; Apc(+/+)) and immo...


    pub_type: 杂志文章


    authors: Fenton JI,Wolff MS,Orth MW,Hord NG

    更新日期:2002-06-01 00:00:00

  • Associations of dietary methyl donor intake with MLH1 promoter hypermethylation and related molecular phenotypes in sporadic colorectal cancer.

    abstract::Intake of dietary factors that serve as methyl group donors may influence promoter hypermethylation in colorectal carcinogenesis. We investigated whether dietary folate, vitamin B2 and vitamin B6, methionine and alcohol were associated with mutL homologue 1 (MLH1) hypermethylation and the related molecular phenotypes ...


    pub_type: 杂志文章


    authors: de Vogel S,Bongaerts BW,Wouters KA,Kester AD,Schouten LJ,de Goeij AF,de Bruïne AP,Goldbohm RA,van den Brandt PA,van Engeland M,Weijenberg MP

    更新日期:2008-09-01 00:00:00

  • Comparative metabolism of N-nitrosopiperidine and N-nitrosopyrrolidine by rat liver and esophageal microsomes and cytochrome P450 2A3.

    abstract::N-nitrosopiperidine (NPIP) is a potent esophageal carcinogen in rats whereas structurally similar N-nitrosopyrrolidine (NPYR) induces liver, but not esophageal tumors. NPIP is a possible causative agent for human esophageal cancer. Our goal is to explain mechanistically these differing carcinogenic activities in the e...


    pub_type: 杂志文章


    authors: Wong HL,Murphy SE,Wang M,Hecht SS

    更新日期:2003-02-01 00:00:00

  • Monoclonal antibodies to 2-amino-1-methyl-6-phenylimidazo[4,5-b] pyridine (PhIP) and their use in the analysis of well-done fried beef.

    abstract::Four monoclonal antibodies, PhIP-1, -2, -3 and -4, have been produced that bind to PhIP (2-amino-1-methyl-6-phenyl-imidazo[4,5-b]pyridine), a mutagenic aromatic amine produced in meat by cooking. The immunogen was an analog of PhIP conjugated to a carrier protein through the 2-amino group of PhIP, leaving the pyridine...


    pub_type: 杂志文章


    authors: Vanderlaan M,Watkins BE,Hwang M,Knize MG,Felton JS

    更新日期:1989-12-01 00:00:00

  • Role of p14ARF in TWIST-mediated senescence in prostate epithelial cells.

    abstract::Recently, TWIST, a basic helix-loop-helix transcription factor, is suggested to be an oncogene because of its over-expression in many types of human cancer and its positive role in promoting cell survival. The aim of this study was to investigate the role of TWIST on the growth of human epithelial cells. Using two imm...


    pub_type: 杂志文章


    authors: Kwok WK,Ling MT,Yuen HF,Wong YC,Wang X

    更新日期:2007-12-01 00:00:00

  • The oncoprotein ErbB3 is endocytosed in the absence of added ligand in a clathrin-dependent manner.

    abstract::The oncoprotein ErbB3 is overexpressed in several human cancers, for example in pancreatic adenocarcinoma and in ovarian cancers, and ErbB3-containing heterodimers have been demonstrated to be potent signaling units in carcinogenesis. This especially applies to ErbB2-ErbB3 and epidermal growth factor receptor (EGFR)-E...


    pub_type: 杂志文章


    authors: Sak MM,Breen K,Rønning SB,Pedersen NM,Bertelsen V,Stang E,Madshus IH

    更新日期:2012-05-01 00:00:00

  • CYP1A1 and GSTM1 genotypes affect benzo[a]pyrene DNA adducts in smokers' lung: comparison with aromatic/hydrophobic adduct formation.

    abstract::Benzo[a]pyrene diol epoxide (BPDE)-DNA adducts are involved in the induction of p53 mutations and probably in the causation of human lung cancer associated with cigarette smoking. The ratio between CYP1A1 and GST enzyme activities is a critical determinant of the target dose of carcinogenic BPDE and other DNA-reactive...


    pub_type: 杂志文章,评审


    authors: Alexandrov K,Cascorbi I,Rojas M,Bouvier G,Kriek E,Bartsch H

    更新日期:2002-12-01 00:00:00

  • A novel experimental model for human mixed acinar-ductal pancreatic cancer.

    abstract::Pancreatic cancer has remained refractory to treatment. In large part, this results from the lack of an animal model that mimics pancreatic cancer in man. We describe a novel experimental model of pancreatic cancer that shares the genetic background, histologic features and natural history of human mixed acinar-ductal...


    pub_type: 杂志文章


    authors: Doiron B,DeFronzo RA

    更新日期:2018-02-09 00:00:00

  • Bioactivation of N-nitrosopiperidine to mutagens: role of hepatic cytochrome P-450 proteins and contribution of cytosolic fraction.

    abstract::In the present study the role of two families of cytochrome P-450 proteins and the contribution of the cytosolic fraction in the activation of N-nitrosopiperidine to mutagens in the Ames test were investigated. The bioactivation of this nitrosamine was preferentially catalysed by the phenobarbitone-induced cytochromes...


    pub_type: 杂志文章


    authors: Ayrton AD,Smith JN,Ioannides C

    更新日期:1987-11-01 00:00:00

  • Dicer regulates non-homologous end joining and is associated with chemosensitivity in colon cancer patients.

    abstract::DNA double-strand break (DSB) repair is an important mechanism underlying chemotherapy resistance in human cancers. Dicer participates in DSB repair by facilitating homologous recombination. However, whether Dicer is involved in non-homologous end joining (NHEJ) remains unknown. Here, we addressed whether Dicer regula...


    pub_type: 杂志文章


    authors: Chen X,Li WF,Wu X,Zhang HC,Chen L,Zhang PY,Liu LY,Ma D,Chen T,Zhou L,Xu Y,Zhou MT,Tang KF

    更新日期:2017-09-01 00:00:00

  • Methylation of the BRCA1 promoter is associated with decreased BRCA1 mRNA levels in clinical breast cancer specimens.

    abstract::Functional inactivation of BRCA1 is an important mechanism involved in breast cancer pathogenesis. Mutation is often responsible for BRCA1 inactivation in familial breast cancer, but is not responsible for the decreased levels of BRCA1 seen in a subset of sporadic breast cancer patients. To determine if aberrant cytos...


    pub_type: 杂志文章


    authors: Rice JC,Ozcelik H,Maxeiner P,Andrulis I,Futscher BW

    更新日期:2000-09-01 00:00:00

  • Unraveling UCA1 lncRNA prognostic utility in urothelial bladder cancer.

    abstract::In the era of precision oncology, bladder cancer (BlCa) is characterized by generic patient management and lack of personalized prognosis and surveillance. Herein, we have studied the clinical significance of urothelial cancer associated 1 (UCA1) lncRNA in improving patients' risk stratification and prognosis. A scree...


    pub_type: 杂志文章


    authors: Avgeris M,Tsilimantou A,Levis PK,Rampias T,Papadimitriou MA,Panoutsopoulou K,Stravodimos K,Scorilas A

    更新日期:2019-08-22 00:00:00

  • Potent suppression of both spontaneous and carcinogen-induced colitis-associated colorectal cancer in mice by dietary celastrol supplementation.

    abstract::Celastrol is an anti-inflammatory natural triterpenoid, isolated from the herb Tripterygium wilfordii or thunder god vine. Here, we define mechanisms mediating anti-inflammatory activity of celastrol and demonstrate efficacy of a dietary celastrol supplement for chemoprevention of inflammation-driven carcinogenesis in...


    pub_type: 杂志文章


    authors: Barker EC,Kim BG,Yoon JH,Tochtrop GP,Letterio JJ,Choi SH

    更新日期:2018-01-12 00:00:00

  • O6-ethylguanine and O6-benzylguanine incorporated site-specifically in codon 12 of the rat H-ras gene induce semi-targeted as well as targeted mutations in Rat4 cells.

    abstract::To examine the miscoding properties of modified guanine residues bearing increasingly bulky O6-substituents, Rat4 cells, grown in the presence of O6-benzylguanine to deplete the DNA repair protein O6-alkylguanine-DNA alkyltransferase, were transfected with plasmids carrying H-ras genes in which O6-methyl, O6-ethyl- an...


    pub_type: 杂志文章


    authors: Bishop RE,Pauly GT,Moschel RC

    更新日期:1996-04-01 00:00:00