Abstract:
:Recent studies have shown opposing effects of n-6 and n-3 fatty acids on the development of cancer and suggest a role for the ratio of n-6 to n-3 fatty acids in the control of cancer. However, whether an alteration in the n-6/n-3 fatty acid ratio of cancer cells affects their invasive potential has not been well investigated. We recently developed a genetic approach to modify the n-6/n-3 ratio by expression of the Caenorhabditis elegans fat-1 gene encoding an n-3 desaturase that converts n-6 to n-3 fatty acids in mammalian cells. The objective of this study was to examine the effect of alteration in the n-6/n-3 fatty acid ratio on the invasive potential of human lung cancer A549 cells. Adenovirus-mediated gene transfer of the n-3 desaturase resulted in a marked reduction of the n-6/n-3 fatty acid ratio, particularly the ratio of arachidonic acid to eicosapentaenic acid. Cell adhesion assay showed that the cells expressing fat-1 gene had a delayed adhesion and retarded colonization. Matrigel assay for invasion potential indicated a 2-fold reduction of cell migration in the fat-1 transgenic cells when compared with the control cells. An increased apoptosis was also observed in the fat-1 transgenic cells. Microarray and quantitative polymerase chain reaction revealed a downregulation of several adhesion/invasion-related genes (MMP-1, integrin-alpha2 and nm23-H4) in the fat-1 transgenic cells. These results demonstrate that a decreased n-6/n-3 fatty acid ratio reduces the invasion potential of human lung cancer cells by probably downregulating the cell adhesion/invasion-related molecules, suggesting a role for the ratio of n-6 to n-3 fatty acids in the prevention and treatment of cancer.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Xia SH,Wang J,Kang JXdoi
10.1093/carcin/bgi019keywords:
subject
Has Abstractpub_date
2005-04-01 00:00:00pages
779-84issue
4eissn
0143-3334issn
1460-2180pii
bgi019journal_volume
26pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::Cancer health disparities remain stubbornly entrenched in the US health care system. The Affordable Care Act was legislation to target these disparities in health outcomes. Expanded access to health care, reduction in tobacco use, uptake of other preventive measures and cancer screening, and improved cancer therapies ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgaa121
更新日期:2020-11-13 00:00:00
abstract::Previously, we reported the identification of a novel immunoglobulin-like cell adhesion molecule hepaCAM that is frequently downregulated and inhibits cell growth in hepatocellular carcinoma. In this study, we show that the expression of hepaCAM is suppressed in diverse human cancers. Aiming to evaluate the biological...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgn226
更新日期:2008-12-01 00:00:00
abstract::Catechol (1,2-dihydroxybenzene) is a major phenolic compound present in the co-carcinogenic fraction of cigarette tar. It has been shown to be a potent co-carcinogen with benzo[a]pyrene (BaP) in mouse skin. In this study we have examined the co-carcinogenic and co-initiating activities of catechol with racemic and ena...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.10.1897
更新日期:1989-10-01 00:00:00
abstract::The transcription factor Sp1 has been implicated in cell-type-specific activation of transforming growth factor-beta (TGFbeta) target genes in normal epithelial cells as well as in aberrant gene activation by TGFbeta in epithelial tumor cells. Here, we have examined the interaction of Sp1 with components of the Smad s...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgl078
更新日期:2006-12-01 00:00:00
abstract::Understanding the molecular pathways that contribute to the development of tamoxifen resistance is a critical research priority as acquired tamoxifen resistance is the principal cause of poor prognosis and death of patients with originally good prognosis hormone-responsive breast tumors. In this report, we provide evi...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs105
更新日期:2012-04-01 00:00:00
abstract::Food-derived aminoimidazoazarenes have been shown to be mutagenic and carcinogenic and to form covalent DNA adducts. 32P-Post-labelling analysis of DNA modified with these heterocyclic amines (HA), including 2-amino-3-methyl-imidazo[4,5-f]quinoline (IQ), 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), 2-amino-...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.5.877
更新日期:1994-05-01 00:00:00
abstract::Liver DNA, obtained at various time intervals from rats chronically fed a choline-devoid diet, was analysed for the presence of aromatic or alkyl adducts by the 32P-postlabeling assay. Alkyl adducts were not detected. Aromatic DNA adduct lesions were revealed, but only at levels (1 adduct per 0.5-3 X 10(9) nucleotides...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.1.187
更新日期:1987-01-01 00:00:00
abstract::In vitro exposures of isolated DNA to one of the two carcinogenic and mutagenic chemicals, diethylsulfate or dimethylsulfate, induces several kinds of physicochemical and morphological alterations. These changes are detectable by a variety of independent techniques. A fraction of DNA treated briefly with either of the...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/2.10.981
更新日期:1981-01-01 00:00:00
abstract::This study was undertaken to investigate the hypothesis linking peroxisome proliferation with the production of reactive oxygen species and subsequent DNA damage. Hepatic peroxisomal proliferation was induced in male Wistar-derived rats by the administration of clofibrate, methyl clofenapate, di(2-ethylhexyl)phthalate...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.9.1213
更新日期:1987-09-01 00:00:00
abstract::The 14-3-3 proteins are associated with proto-oncogene and oncogene products. Here, we generated NIH 3T3 cells overexpressing the beta isoform of the 14-3-3 proteins (14-3-3 beta) to examine the function of this isoform in cellular proliferation and oncogenic transformation. Overexpression of 14-3-3 beta in NIH 3T3 ce...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.11.2073
更新日期:2000-11-01 00:00:00
abstract::Administration of methylglyoxal at doses of 300-600 mg/kg body weight by gastric tube to male F344 rats induced 100-fold increase in ornithine decarboxylase activity (formation of 682 pmol CO2/30 min/mg protein) within 7 h, 26-fold increase in DNA synthesis (incorporation of 17 800 d.p.m. of [3H]thymidine/microgram DN...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.1.91
更新日期:1985-01-01 00:00:00
abstract::There is increasing concern that compounds formed during the chemical or biological transformation of pollutants in the environment may be more detrimental to human and environmental health than the original pollutant. In this study, two bacterial transformation products of polycyclic aromatic hydrocarbons (PAHs), pyr...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh174
更新日期:2004-09-01 00:00:00
abstract::We have shown previously that apoptosis induction by diallyl trisulfide (DATS), a constituent of processed garlic, in PC-3 and DU145 human prostate cancer cells is associated with c-Jun N-terminal kinase and extracellular signal-regulated kinase-mediated phosphorylation of Bcl-2. However, pharmacological inhibition of...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi228
更新日期:2006-03-01 00:00:00
abstract::Hypoxia-inducible factors (HIFs), in particular HIF-1alpha, have been implicated in tumor biology. However, HIF target genes in the esophageal tumor microenvironment remain elusive. Gene expression profiling was performed upon hypoxia-exposed non-transformed immortalized human esophageal epithelial cells, EPC2-hTERT, ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgp326
更新日期:2010-03-01 00:00:00
abstract::In the previous 500 2-year chemical bioassays within the National Toxicology Program, large intestinal tumors (cecal carcinomas) related to chemical exposure have not been observed in B6C3F1 mice. The recently completed o-nitrotoluene study provided the first cecal tumor response and an opportunity to evaluate the mor...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh044
更新日期:2004-04-01 00:00:00
abstract::Sodium arsenite and sodium arsenate were observed to induce morphological transformation of Syrian hamster embryo cells in a dose-dependent manner. A linear dose-dependence with a slope of approximately 1 was observed with both compounds when the data were plotted on a log-log graph. The trivalent sodium arsenite was ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.10.1421
更新日期:1985-10-01 00:00:00
abstract::We investigated, in a cloned hamster tracheal epithelial cell line HTE-B, the effects of inhibitors of DNA topoisomerase, novobiocin and nalidixic acid; of DNA polymerase, 1-beta-arabinofuranosylcytosine (ara-C) and 2',3'-dideoxythymidine; of ribonucleotide reductase, hydroxyurea; and of poly(ADP-ribose)synthetase, 3-...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/5.2.187
更新日期:1984-02-01 00:00:00
abstract::Flow cytometric analysis of fibroblasts, normal breast epithelial cells and breast or other cancer cell lines identified variation in the abilities of cell lines to undergo cell cycle arrest as a response to hypoxia. Human mammary epithelial cells (HMEC), normal fibroblasts (Hs68 and WI38), HeLa cervical carcinoma and...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgh274
更新日期:2004-12-01 00:00:00
abstract::The LEC rat spontaneously develops liver cancer after suffering chronic liver injury caused by abnormal copper accumulation in the liver, but the role of copper accumulation in the induction of liver cancer remains obscure. We histochemically and biochemically examined the content of copper and metallothionein (MT), a...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.9.1833
更新日期:1994-09-01 00:00:00
abstract::The mutagenicity of N-nitroso-N-benzyl-methylamine (NBzMA), N-benzyl-N-nitrosourea (BzNU) and N-methyl-N-nitrosourea (MNU) in Salmonella typhimurium strains was investigated. BzNU selectively mutated TA100 strain as compared to TA1535, whereas MNU showed an inverse strain response, an effect probably related to the fa...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.9.1653
更新日期:1990-09-01 00:00:00
abstract::Normal human fibroblasts do not synthesize platelet-derived growth factor (PDGF), but they respond to its mitogenic action. In contrast, cells from several human fibrosarcomas have been shown to synthesize PDGF at significant levels. To investigate the possible role of PDGF expression in the development of tumors of m...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.10.2167
更新日期:1994-10-01 00:00:00
abstract::Male Ah-responsive C57BL/10ScSn mice received a single dose of iron-dextran (600 mg Fe/kg) and were fed a diet containing 0.01% of the PCBs mixture Aroclor 1254 for up to 12 months. Iron caused a marked synergistic increase in liver size from 2 months with greatly elevated mitotic rates, numbers of basophilic foci and...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/11.3.437
更新日期:1990-03-01 00:00:00
abstract::Expression of the human placental form of glutathione S-transferase (GST-pi), an enzyme proposed as a marker for human and experimental neoplasia, was immunohistochemically evaluated in 51 samples of 'normal' and diseased adult human uterine cervix. Five fetal uteri were also studied. GST-pi positivity was detected in...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/10.12.2317
更新日期:1989-12-01 00:00:00
abstract::Cancer-associated fibroblasts (CAFs) have been considered to play an important role for tumor progression of cancer. Solid tumors contain heterogeneous distribution of oxygen in their microenvironments. This study investigated the growth signaling of gastric cancer (GC) cells in focus on the interaction with CAFs and ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgv134
更新日期:2015-12-01 00:00:00
abstract::Inflammation is highly associated with colon carcinogenesis. Epigenetic mechanisms could play an important role in the initiation and progression of colon cancer. Curcumin, a dietary phytochemical, shows promising effects in suppressing colitis-associated colon cancer in azoxymethane-dextran sulfate sodium (AOM-DSS) m...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgy043
更新日期:2018-05-03 00:00:00
abstract::Diethylstilbestrol (DES), a synthetic stilbene estrogen, is a potent development toxin and carcinogen in humans and rodents. A number of 32P-postlabeling studies suggest that genotoxic effects of DES substantially contribute to these biological effects. The mechanisms involved in DES-mediated genotoxicity are not comp...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/16.11.2643
更新日期:1995-11-01 00:00:00
abstract::Cytochrome P4501A1 (CYP1A1) has been implicated in the conversion of numerous polycyclic aromatic hydrocarbons into electrophilic species capable of binding covalently to DNA and has therefore been postulated to be involved in the initiation of carcinogenesis. The expression of CYP1A1 protein appears not to be constit...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.8.1361
更新日期:1998-08-01 00:00:00
abstract::Maternal nutrition during pregnancy influences the development and metabolism of the fetus. Recent studies suggest that the cancer risk of offspring later in life is associated with maternal diet, but little is known about the effect of a maternal diet high in methyl nutrients on breast cancer risk. Lipotropes are met...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgs125
更新日期:2012-05-01 00:00:00
abstract::Application of 5 nmol 12-O-tetradecanoylphorbol-13-acetate (TPA) to the skin of female CD-1 mice led to a rapid increase in the concentration of epidermal ornithine decarboxylase (ODC) mRNA from an undetectable level in control mice to a high maximum level at 4-5 h after TPA administration. The concentration of epider...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.2.293
更新日期:1993-02-01 00:00:00
abstract::Previous studies have shown that platinum-DNA adduct level in leukocyte DNA (measured by antibody methodology) is directly related to disease response in ovarian cancer and testicular cancer. To determine if this principle could be more broadly applied, platinum-DNA damage was studied in a blinded fashion in leukocyte...
journal_title:Carcinogenesis
pub_type: 临床试验,杂志文章
doi:10.1093/carcin/12.7.1253
更新日期:1991-07-01 00:00:00