Abstract:
:The localization of benzo[a]pyrene-deoxyguanosine adducts was studied by indirect immunofluorescence in cultured BALB/c epidermal cells exposed to (+/-) 7 alpha, 8 alpha-dihydroxy-9 alpha, 10 alpha-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene (the anti-isomer) utilizing an antiserum specific for the major benzo[a]pyrene-deoxyguanosine adduct in DNA. This antiserum does not cross-react with benzo[a]pyrene or DNA alone. When cultured keratinocytes were incubated with the carcinogen for 1 h, the immunofluorescence was localized in the nucleus as intense spots on a background of diffuse fluorescence. Fluorescence was absent from cells not exposed to carcinogen and from carcinogen-exposed cells incubated with normal rabbit serum in place of the antiserum. Fluorescence was abolished when the specific antiserum was absorbed with the immunogen DNA prior to incubation with cells, and substantially diminished when exposed cells were preincubated with deoxyribonuclease before the application of the specific antiserum. Incubation of exposed cells with ribonuclease prior to incubation with the specific antiserum removed the bright fluorescent spots and resulted in fluorescent nuclei containing dark sports in similar frequency. Dose-response studies in which benzo[a]pyrene-deoxyguanosine adducts were quantified by enzyme-linked immunosorbent assay and compared with intensity of immunofluorescence demonstrated that decreasing doses of the carcinogen resulted in fewer numbers of adducts as well as proportionally less fluorescence. When cells were exposed to non-toxic doses of the activated carcinogen for 1 h, nuclear fluorescence was detectable in immediately-fixed cells but faded to non-detectable levels when cells were washed and cultured for an additional 24-48 h before fixation.
journal_name
Carcinogenesisjournal_title
Carcinogenesisauthors
Poirier MC,Stanley JR,Beckwith JB,Weinstein IB,Yuspa SHdoi
10.1093/carcin/3.3.345subject
Has Abstractpub_date
1982-01-01 00:00:00pages
345-8issue
3eissn
0143-3334issn
1460-2180journal_volume
3pub_type
杂志文章相关文献
CARCINOGENESIS文献大全abstract::Epidemiological studies have linked consumption of broccoli to a reduced risk of colon cancer in individuals with the glutathione S-transferase M1 (GSTM1) null genotype. GSTs are involved in excretion and elimination of isothiocyanates (ITCs), which are major constituents of broccoli and other cruciferous vegetables a...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/21.12.2287
更新日期:2000-12-01 00:00:00
abstract::Phosphoinositide-specific phospholipase Cgamma (PLCgamma) is a key regulatory enzyme that binds to the phosphoryl-tyrosine residues in the cytoplasmic domain of certain activated receptors and catalyses the hydrolysis of phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] forming IP3 and diacylglycerol (DAG) in resp...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.1.177
更新日期:1998-01-01 00:00:00
abstract::The expression of CYP1B1 in human mammary fibroblasts (HMFs) was characterized as a potential modulator of their individual function as well as effects on adjacent mammary epithelia. We have used these characteristics to explore the diversity of fibroblast cells isolated from reduction mammoplasty patients and from di...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/19.8.1437
更新日期:1998-08-01 00:00:00
abstract::The rat liver 9000 g supernatant mediated metabolism of the carcinogenic aristolochic acid, which consists of aristolochic acid I (AAI) and aristolochic acid II (AAII), was investigated. Under anaerobic conditions the major metabolites were the corresponding aristolactams for both AAI and AAII. In contrast under aerob...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.1.59
更新日期:1986-01-01 00:00:00
abstract::The role of nitric oxide (NO) in colon cancer remains controversial. Inducible nitric oxide synthase (iNOS) has been reported to be up regulated and down regulated in colorectal cancer in both animal models and patient tissue samples. Cyclooxygenase-2 (COX-2) is important in colorectal carcinogenesis but its relations...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgg014
更新日期:2003-04-01 00:00:00
abstract::Metabolism and nucleic acid binding of the mammary gland carcinogen N-hydroxy-4-acetylaminobiphenyl (N-OH-AABP) was investigated using the human mammary epithelial cell line MCF 10A. Chromatographic analysis of the ethyl acetate extract of the media from cultured MCF 10A after 24 h exposure to N-OH-AABP revealed the f...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/15.4.611
更新日期:1994-04-01 00:00:00
abstract::Basic in vitro systems can be used to model and assess complex diseases, such as cancer. Recent advances in this field include the incorporation of multiple cell types and extracellular matrix proteins into three-dimensional (3D) models to recapitulate the structure, organization and functionality of live tissue in si...
journal_title:Carcinogenesis
pub_type: 杂志文章,评审
doi:10.1093/carcin/bgu108
更新日期:2014-08-01 00:00:00
abstract::Strains of laboratory mice vary markedly in their susceptibility to two-stage skin carcinogenesis using 7,12-dimethylbenz[a]anthracene (DMBA) as the initiating carcinogen and croton oil as promoter. This study has been undertaken in order to clarify the basis of the strain differences. LACA mice were used as the susce...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/3.1.99
更新日期:1982-01-01 00:00:00
abstract::Administration of 2-acetylaminofluorene (2-AAF) to rats increased mdr1b expression in Fischer, Wistar and Sprague-Dawley rat livers; however, the response was much smaller in Sprague-Dawley livers. To investigate the basis of this difference we further examined the regulation of the mdr1b gene in hepatocytes isolated ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.3.451
更新日期:1996-03-01 00:00:00
abstract::Some of the glutathione S-transferases (GSTs) are polymorphic and may play a role in lung cancer susceptibility. Our previous study in a French Caucasian study population suggested GSTM1 null genotype as a moderate risk factor for lung cancer. Here we extended the study to investigate the potential role of GSTT1 and G...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/23.9.1475
更新日期:2002-09-01 00:00:00
abstract::Sodium arsenite and sodium arsenate were observed to induce morphological transformation of Syrian hamster embryo cells in a dose-dependent manner. A linear dose-dependence with a slope of approximately 1 was observed with both compounds when the data were plotted on a log-log graph. The trivalent sodium arsenite was ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/6.10.1421
更新日期:1985-10-01 00:00:00
abstract::Apurinic/apyrimidinic endonuclease/redox effector factor-1 (APE/Ref-1) is a multifunctional protein involved both in DNA base excision repair and redox regulation. Studies have suggested that abnormal Ref-1 levels and/or activities are associated with tumor progression and sensitivities to treatment, but no direct evi...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgm128
更新日期:2007-11-01 00:00:00
abstract::Oesophageal squamous cell carcinoma (OSCC) has a high incidence in southern Africa and a poor prognosis. Limited information is available on the contribution of genetic variants in susceptibility to OSCC in this region. However, recent genome-wide association studies have identified multiple susceptibility loci in Asi...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgz026
更新日期:2019-06-10 00:00:00
abstract::Long-Evans Cinnamon (LEC) rats, a model for human Wilson's disease, develop chronic hepatitis and liver tumors owing to accumulation of copper and induced oxidative stress. Lipid peroxidation (LPO)-induced etheno-DNA adducts in nuclear- and mitochondrial-DNA along with apoptosis was measured in LEC rat liver. Levels o...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi073
更新日期:2005-07-01 00:00:00
abstract::The effect of phenobarbital (PB), p,p'-dichlorodiphenyltrichloroethane (DDT) or carbon tetrachloride (CCl4) on dimethylnitrosamine (DMN)-induced hepatocarcinogenesis in Syrian golden hamsters was examined. Hamsters were given a single injection of DMN (6 mg/kg body wt) followed by either PB or DDT in the diet or repea...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.9.1171
更新日期:1987-09-01 00:00:00
abstract::Xanthorrhizol is an active component isolated from Curcuma xanthorrhiza Roxb. (Zingiberaceae) that is traditionally used in Indonesia for medicinal purposes. In the present study, we found that the topical application of xanthorrhizol before 12-O-tetradecanoylphorbol-13-acetate (TPA) treatment significantly inhibits T...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgm005
更新日期:2007-06-01 00:00:00
abstract::Aloe-emodin (AE), one of the main bioactive anthraquinones of Rheum palmatum, possesses potent antitumor properties. Our previous proteomic study revealed that AE-induced apoptosis was associated with oxidative stress and oxidation of many redox-sensitive proteins. In this study, we aimed to further dissect the cell d...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgm143
更新日期:2007-09-01 00:00:00
abstract::We have studied the influence of genetic polymorphisms in the xenobiotic-metabolizing genes GSTM1, GSTP1, GSTT1, EPHX1, NAT1 and NAT2 and the folate-metabolizing genes MTR and MTHFR on the frequencies of cells with chromosomal aberrations (CAs) in peripheral lymphocytes of Norwegian men. Log-linear Poisson regression ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgq246
更新日期:2011-03-01 00:00:00
abstract::Autoradiography has been used to study the effect of 12-O-tetradecanoylphorbol-13-acetate (TPA), 4-O-methyl TPA, and phorbol on metabolic cooperation between human diploid fibroblasts. When the donors, hypoxanthine-guanine phosphoribosyl transferase+ (HGPRT+) cells, and recipients, HGPRT- cells, were plated together i...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/3.10.1207
更新日期:1982-01-01 00:00:00
abstract::The effect of co-administration of diethylnitrosamine (DEN) and Clophen A 50, a commercial mixture of polychlorinated biphenyls (PCB), on pre-neoplastic enzyme-altered islands in livers of female Sprague-Dawley rats was studied. The islands were identified by the loss of adenosine-5'-triphosphatase (ATPase), emergence...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/7.10.1697
更新日期:1986-10-01 00:00:00
abstract::We have shown previously that apoptosis induction by diallyl trisulfide (DATS), a constituent of processed garlic, in PC-3 and DU145 human prostate cancer cells is associated with c-Jun N-terminal kinase and extracellular signal-regulated kinase-mediated phosphorylation of Bcl-2. However, pharmacological inhibition of...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi228
更新日期:2006-03-01 00:00:00
abstract::Spices and flavoring plants part rich in supposedly health-promoting phytochemicals are currently receiving much attention as a possible source of cancer chemopreventive compounds. Clove, the sun-dried unopened flower bud from the plant Syzygium aromaticum L. is a commonly used spice and food flavor. In the present wo...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi372
更新日期:2006-08-01 00:00:00
abstract::The metabolism of [1,3-14C]benzo[f]quinoline (BfQ) by liver microsomes from control, 3-methylcholanthrene (3-MC)-pretreated and phenobarbital (PB)-pretreated rats has been investigated in order to gain insights into the effect of mixed function oxidase inducers on the types and levels of specific metabolites as formed...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/8.12.1861
更新日期:1987-12-01 00:00:00
abstract::Recent studies have implicated multipotential mesenchymal stem cells (MSCs) as an aid to breast cancer cell proliferation and metastasis, partly as a result of the MSCs secretome. As the tumor gets beyond 2 mm in diameter, the stromal cells could undergo starvation due to the lack of sufficient nutrients in solid tumo...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgr029
更新日期:2011-07-01 00:00:00
abstract::The broccoli constituent sulforaphane (1-isothiocyanate-4-methylsulfinylbutane) has previously been shown to protect rats against 9,10-dimethyl-1,2-benz[a]anthracene tumorigenesis, thought to be due, at least in part, to induction of phase II detoxification. We investigated the ability of sulforaphane to also inhibit ...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/17.2.277
更新日期:1996-02-01 00:00:00
abstract::Metastasis-supporting physiological alterations are regulated by cell signaling molecules, which target signal transduction pathways and gene expression. Osteopontin (OPN) overexpression may represent a key molecular event in cancer metastasis. In this study, using metastatic 4T1 and non-metastatic 4T07 murine mammary...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi352
更新日期:2006-06-01 00:00:00
abstract::Common polymorphisms in genes encoding phase I and phase II enzymes are considered to modify lung cancer risk due to changes in enzyme activity. Candidates include genetic variants of glutathione S-transferases (GSTM1, GSTT1 and GSTP1) and myeloperoxidase (MPO). We performed a large case-control study of these candida...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/bgi227
更新日期:2006-03-01 00:00:00
abstract::The metabolism of 7,12-dimethylbenz[a]anthracene (DMBA), benzo[a]pyrene (BP) and benz[a]anthracene (BA) by epithelial cell aggregates and fibroblasts in culture has been investigated using mammary tissue obtained from female Wistar rats. The results show that: (a) both types of mammary cells metabolise all three hydro...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/3.2.203
更新日期:1982-01-01 00:00:00
abstract::The haloethylnitrosoureas form a cytotoxic DNA cross-link in a series of reactions which involves initial alkylation of the O6 position of guanine and rearrangement to the intermediate, 1,O6-ethanoguanine; 1,O6-ethanoguanine then reacts with a neighboring cytosine base. O6-Alkylguanine-DNA alkyltransferase can interru...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/14.2.195
更新日期:1993-02-01 00:00:00
abstract::Aphidicolin is a specific inhibitor of DNA polymerase alpha. Its influence of DNA repair has been studied in both normal and excision deficient xeroderma pigmentosum cells exposed to u.v. irradiation at 254 nm. Single strand DNA breaks accumulated in u.v. irradiated normal cells when the inhibitor was present. Such br...
journal_title:Carcinogenesis
pub_type: 杂志文章
doi:10.1093/carcin/2.8.795
更新日期:1981-01-01 00:00:00