Abstract:
:A receptor specific for lipoglycans from Acholeplasma axanthum and Acholeplasma granularum was isolated from sheep erythrocyte stroma by extraction with n-pentanol and permeation chromatography. The purified receptor appeared as one band on sodium dodecyl sulfate-polyacrylamide gels and stained with Coomassie blue, periodate-Schiff reagent, and Sudan black. It was distinct from the erythrocyte receptor for gram-negative lipopolysaccharides and the glycophorin receptor for certain species of Mycoplasma. Periodate oxidation and trypsin did not affect the receptor activity in intact erythrocytes, but the purified receptor was susceptible to proteolytic digestion. Specific receptors, sensitive to trypsin digestion, could be isolated from rabbit kidney and cultured rabbit epidermal cell membranes. These could be distinguished from the receptor from erythrocytes by their solubility in n-pentanol. The segment of the lipoglycan molecule which binds to these receptors was not lipoidal in nature and was distinct from the specific antigenic determinants of the lipoglycans.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Al-Samarrai TH,Smith PF,Lynn RJdoi
10.1128/IAI.38.3.1078-1087.1982subject
Has Abstractpub_date
1982-12-01 00:00:00pages
1078-87issue
3eissn
0019-9567issn
1098-5522journal_volume
38pub_type
杂志文章abstract::Alveolar macrophages are the effector cells largely responsible for clearance of Pneumocystis carinii from the lungs. Binding of organisms to beta-glucan and mannose receptors has been shown to stimulate phagocytosis of the organisms. To further define the mechanisms used by alveolar macrophages for clearance of P. ca...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00393-07
更新日期:2007-08-01 00:00:00
abstract::The biological activity of C-ASWS, an alkali-soluble, water-soluble cell wall antigen isolated from mycelial-phase cells of Coccidioides immitis, was compared with that of a commercial coccidioidin (CDN; Cutter Laboratories); CDN-TS, a toluene-induced lysate of mycelial-phase cells; and spherulin, a spherule-derived e...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.3.751-755.1977
更新日期:1977-03-01 00:00:00
abstract::Curli are thin, coiled fibers expressed on the surface of Escherichia coli that bind several matrix and plasma proteins such as fibronectin, laminin, plasminogen, tissue plasminogen activator, and H-kininogen. In this work, we examined the interactions between curli-expressing E. coli and human major histocompatibilit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.3.944-949.1998
更新日期:1998-03-01 00:00:00
abstract::Ers has been qualified as the PrfA-like transcriptional regulator of Enterococcus faecalis. In a previous study we reported that Ers is important for the survival within macrophages of this opportunist pathogenic bacterium. In the present work we have used proteomic and microarray expression profiling of E. faecalis J...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00161-08
更新日期:2008-07-01 00:00:00
abstract::Among a number of laboratory strains of Listeria monocytogenes used in experimental infection, strain LO28 is highly capable of inducing robust beta interferon (IFN-β) production in infected macrophages. In this study, we investigated the molecular mechanism of the IFN-β-inducing ability of LO28 by comparing it with t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06162-11
更新日期:2012-07-01 00:00:00
abstract::Leptospira interrogans is an important mammalian pathogen. Transmission from an environmental source requires adaptations to a range of new environmental conditions in the organs and tissues of the infected host. Since many pathogenic bacteria utilize temperature to discern their environment and regulate the synthesis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.400-404.2001
更新日期:2001-01-01 00:00:00
abstract::Human opsonins directed against specific meningococcal outer membrane structures in sera obtained during meningococcal disease were quantified with a recently developed antigen-specific, opsonin-dependent phagocytosis and oxidative burst assay. Outer membrane vesicles (OMVs) and PorA (class 1) and PorB (class 3) prote...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.5.2552-2560.1999
更新日期:1999-05-01 00:00:00
abstract::We constructed the expression vector pSK-SCP containing the streptococcal exotoxin B gene (spe b) which expressed protease activity. We showed that the recombinant streptococcal pyogenic exotoxin B/streptococcal cysteine protease (rSPE B/SCP) was secreted into the culture supernatant of the transformant and retained i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.7.3944-3947.2002
更新日期:2002-07-01 00:00:00
abstract::Heart and muscle tissues appear to be specific target organs for the in vivo inhibition of protein synthesis by diphtheria toxin. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.5.686-688.1970
更新日期:1970-11-01 00:00:00
abstract::Enterohemorrhagic Escherichia coli (EHEC) is a prominent, food-borne cause of diarrhea, bloody diarrhea, and the hemolytic uremic syndrome in industrialized countries. Most strains of EHEC carry the locus for enterocyte effacement (LEE) pathogenicity island, but a proportion of isolates from patients with severe disea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.11.6307-6319.2003
更新日期:2003-11-01 00:00:00
abstract::A high-molecular-weight, immunogenic form of the lipopolysaccharide O side chain of Pseudomonas aeruginosa Fisher immunotype 4 (type 4, International Antigenic Typing System 1, Lanyi O:6) was isolated and characterized. Analysis by nuclear magnetic resonance spectroscopy confirmed the structural similarity of this hig...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.2.426-431.1989
更新日期:1989-02-01 00:00:00
abstract::The bacterium Listeria monocytogenes causes meningoencephalitis in humans. In rodents, listeriosis is associated with granulomatous lesions in the liver and the spleen, but not with meningoencephalitis. Here, infant rats were infected intracisternally to generate experimental listeric meningoencephalitis. Dose-depende...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.4086-4093.2001
更新日期:2001-06-01 00:00:00
abstract::Staphylococcus aureus is a pathogen that causes significant morbidity and mortality. Nasal carriage is a major source of transmission and of endogenous infection. Persistent carriage is detected in ∼30% of healthy individuals. While Th17 cells have been shown to play a role in S. aureus infection and clearance, the im...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00834-17
更新日期:2018-02-20 00:00:00
abstract::Inactivated Marek's disease virus-infected chicken kidney cells and inactivated MSB-1 lymphoblastoid Marek's disease tumor cells were used to immunize chickens as virus- and tumor-associated antigens, respectively. Immune and nonimmune birds were then challenged by exposure to live virulent Marek's disease virus. Both...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.2.547-553.1979
更新日期:1979-11-01 00:00:00
abstract::The literature on chronic mucocutaneous candidiasis contains multiple reports which suggest that loss of cell-mediated immunity in this disease may be related in part to the presence of an inhibitory factor(s) present in patient plasma. One such inhibitory factor has been suggested to be mannan polysaccharide released...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.3.1041-1046.1984
更新日期:1984-03-01 00:00:00
abstract::Exposure to bacterial superantigens such as staphylococcal enterotoxin B (SEB) leads to the induction of toxic shock syndrome which results in multiorgan failure, including liver damage. In the present study, we investigated the role of CD44 in SEB-induced liver injury. Injection of SEB into d-galactosamine-sensitized...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.50-61.2005
更新日期:2005-01-01 00:00:00
abstract::Differences in acid tolerance among representative oral streptococci were found to be related more closely to the dynamic permeabilities of the bacteria to protons than to differences in the sensitivities of cell membranes to gross damage caused by environmental acidification. For Streptococcus mutans GS-5, Streptococ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.2.331-338.1986
更新日期:1986-08-01 00:00:00
abstract::Host-driven macrophage apoptosis contributes to innate immunity during bacterial infection. Neisseria meningitidis inhibits apoptosis in a variety of cells, but its impact on macrophage apoptosis is unknown. We demonstrate that N. meningitidis prevents macrophage apoptosis via genes encoding nitric oxide detoxificatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.1.729-733.2006
更新日期:2006-01-01 00:00:00
abstract::To evaluate the roles of the infectious agent and the host in the pathogensis of Salmonella-associated arthritis (SAA), 1O(3) to 10(6) heat-killed Salmonella enteritidis were injected directly into involved carpal joints in normal and actively immunized rats and in hosts adoptively immunized by the intravenous transfe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.4.1155-1160.1976
更新日期:1976-04-01 00:00:00
abstract::Antibodies to Escherichia coli O and K antigens were raised in rabbits by repeated immunizations with whole, Formalin-killed and, later, liver bacteria. The serum antibody levels were determined with the ammonium sulfate precipitation technique after radioiodinating the antigens. The K antigens had to be conjugated to...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.2.286-289.1977
更新日期:1977-08-01 00:00:00
abstract::A total of 224 strains were serogrouped by coagglutination (COA) and serotyped by protein I enzyme-linked immunosorbent assay (ELISA). Of these strains, 61 were from patients with disseminated gonococcal infection, 21 were from patients with pelvic inflammatory disease, and 115 were from patients with uncomplicated go...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.1.229-239.1982
更新日期:1982-01-01 00:00:00
abstract::Recurrent pulmonary infections with Staphylococcus aureus and Pseudomonas aeruginosa are a major problem in cystic fibrosis (CF), leading to severe and progressive deterioration of the respiratory tract. Fibronectin (FN) has a binding site(s) for S. aureus and Streptococcus pyogenes. This bacterial bindings site(s) wa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.3.949-954.1991
更新日期:1991-03-01 00:00:00
abstract::Biomaterial-associated infections (BAI), which are predominantly caused by Staphylococcus epidermidis, are a significant problem in modern medicine. Biofilm formation is considered the pivotal element in the pathogenesis, but in previous mouse studies we retrieved S. epidermidis from peri-implant tissue. To assess the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01262-06
更新日期:2007-03-01 00:00:00
abstract::The ability of Streptococcus salivarius strain TOVE-R to inhibit the ecological emergence of virulent representatives of the most prevalent human mutans streptococci on the teeth of specific pathogen-free Osborne-Mendel rats was studied. Rats which were infected by TOVE-R, or either S. mutans 10449S or S. sobrinus 671...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.1.76-83.1985
更新日期:1985-07-01 00:00:00
abstract::To investigate arthritis-inducing properties of Eubacterium species, which are major residents of the human intestinal flora, cell wall fragments (CWF) of several Eubacterium strains were prepared and tested in an animal model. After a single intraperitoneal injection in the rat, CWF of E. aerofaciens, E. contortum, a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.2.523-528.1990
更新日期:1990-02-01 00:00:00
abstract::We have previously shown that a Pasteurella multocida cexA mutant (PBA875) was impaired in capsule export and highly attenuated in virulence for mice (J. D. Boyce and B. Adler, Infect. Immun. 68:3463-3468, 2000). In this study we show that immunization with high, but not low, doses of PBA875 can confer significant pro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1943-1946.2001
更新日期:2001-03-01 00:00:00
abstract::When tested for their activity in suppressing the acquisition of immunity during acute Besnoitia infection of hamsters, antilymphocyte serum (ALS), aminopterin, cyclophosphamide, cortisol, and whole-body irradiation were the most active agents and effectively blocked the development of immunity during 4 to 12 days of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.6.756-761.1971
更新日期:1971-06-01 00:00:00
abstract::The mechanism of activation of intestinal guanylate cyclase by Escherichia coli heat-stable enterotoxin (STa) has been studied by using isolated rat intestinal epithelial cells and purified brush border membrane (BBM) preparations. Inhibitors of prostaglandin biosynthesis, quinacrine and 5,8,11,14-eicosatetraynoic aci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.2.493-501.1984
更新日期:1984-05-01 00:00:00
abstract::Mice receiving a single injection of cyclophosphamide (150 mg/kg) 1 to 6 days before inoculation with viable Candida albicans showed an increased susceptibility to the challenge accompanied by a reduction in peripheral blood polymorphonuclear leukocytes and lymphocytes as well as in spleen cellularity. Several immunol...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.1.46-55.1983
更新日期:1983-04-01 00:00:00
abstract::Previous reports from our laboratory have shown that 7 days after infection of C3HeB/FeJ mice with an attenuated strain of Salmonella typhimurium, there is profound suppression of responses to B- and T-cell mitogens and suppression of the capacity of spleen cells to mount a primary, in vitro plaque-forming-cell (PFC) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.9.3786-3792.1996
更新日期:1996-09-01 00:00:00