Isolation and characterization of the sheep erythrocyte receptor for acholeplasmal lipoglycans.

Abstract:

:A receptor specific for lipoglycans from Acholeplasma axanthum and Acholeplasma granularum was isolated from sheep erythrocyte stroma by extraction with n-pentanol and permeation chromatography. The purified receptor appeared as one band on sodium dodecyl sulfate-polyacrylamide gels and stained with Coomassie blue, periodate-Schiff reagent, and Sudan black. It was distinct from the erythrocyte receptor for gram-negative lipopolysaccharides and the glycophorin receptor for certain species of Mycoplasma. Periodate oxidation and trypsin did not affect the receptor activity in intact erythrocytes, but the purified receptor was susceptible to proteolytic digestion. Specific receptors, sensitive to trypsin digestion, could be isolated from rabbit kidney and cultured rabbit epidermal cell membranes. These could be distinguished from the receptor from erythrocytes by their solubility in n-pentanol. The segment of the lipoglycan molecule which binds to these receptors was not lipoidal in nature and was distinct from the specific antigenic determinants of the lipoglycans.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Al-Samarrai TH,Smith PF,Lynn RJ

doi

10.1128/IAI.38.3.1078-1087.1982

subject

Has Abstract

pub_date

1982-12-01 00:00:00

pages

1078-87

issue

3

eissn

0019-9567

issn

1098-5522

journal_volume

38

pub_type

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