Impairment of protein synthesis in the retinal tissue in diabetic rabbits: secondary reduction of fast axonal transport.

abstract：:Nerve growth factor (NGF) is a trophic and survival factor for cholinergic neurons, and it induces the expression of several genes that are essential for synthesis and storage of acetylcholine (ACh), specifically choline acetyltransferase, vesicular ACh transporter (VAChT), and choline transporter. We have found previ...

journal_title：Journal of neurochemistry

authors： Madziar B,Shah S,Brock M,Burke R,Lopez-Coviella I,Nickel AC,Cakal EB,Blusztajn JK,Berse B

更新日期：2008-12-01 00:00:00

abstract：:Primary astrocyte cultures from neonatal rat brains show uptake of [3H]norepinephrine ([3H]NE). This uptake has a high-affinity component with an apparent Km of approximately 3 X 10(-7) M. At 10(-7) M [3H]NE both the initial rate of uptake and steady-state content of [3H]NE is inhibited by up to 95% by omission of ext...

journal_title：Journal of neurochemistry

authors： Kimelberg HK,Pelton EW 2nd

更新日期：1983-05-01 00:00:00

abstract：:In Aplysia, serotonin mediates behavioral sensitization by increasing the strength of the synapse between sensory and motor neurons, a process known as facilitation. The retention of long-term facilitation is blocked by rapamycin, an inhibitor of a specific translational pathway. One possible rapamycin-sensitive targe...

journal_title：Journal of neurochemistry

authors： Carroll M,Warren O,Fan X,Sossin WS

更新日期：2004-09-01 00:00:00

abstract：:The in vitro and in vivo performance of three different semipermeable microdialysis membranes was compared: a proprietary polycarbonate-ether membrane made by Carnegie Medecin; cuprophan, a regenerated cellulose membrane; and polyacrylonitrile. When microdialysis probes were tested in a stirred in vitro solution, larg...

journal_title：Journal of neurochemistry

authors： Hsiao JK,Ball BA,Morrison PF,Mefford IN,Bungay PM

更新日期：1990-04-01 00:00:00

abstract：:The overflows of 3,4-dihydroxyphenylalanine, dopamine, noradrenaline, and 3,4-dihydroxyphenylglycol in canine portal vein superfused in vitro were studied before, during, and after depolarization of sympathetic nerve endings. The four compounds were separated from superfusate and from tissue on Sep-Pak C-18 cartridges...

journal_title：Journal of neurochemistry

authors： Hunter LW,Rorie DK,Tyce GM

更新日期：1992-09-01 00:00:00

abstract：:Frontotemporal lobar degeneration (FTLD) is the second leading cause of dementia in individuals under age 65. In many patients, the predominant pathology includes neuronal cytoplasmic or intranuclear inclusions of ubiquitinated TAR DNA binding protein 43 (FTLD-TDP). Recently, a genome-wide association study identified...

journal_title：Journal of neurochemistry

authors： Nicholson AM,Finch NA,Wojtas A,Baker MC,Perkerson RB 3rd,Castanedes-Casey M,Rousseau L,Benussi L,Binetti G,Ghidoni R,Hsiung GY,Mackenzie IR,Finger E,Boeve BF,Ertekin-Taner N,Graff-Radford NR,Dickson DW,Rademakers R

更新日期：2013-09-01 00:00:00

abstract：:U-78518F, a 21-aminosteroid from the novel family of lipid peroxidation inhibitors (lazaroids), increased survival of dopamine (DA) neurons in mesencephalic cell cultures incubated with the neurotoxin 1-methyl-4-phenylpyridinium (MPP+). Protection against DA neuron death occurred with increasing concentrations of U-78...

journal_title：Journal of neurochemistry

authors： Sanchez-Ramos JR,Song S,Mash DC,Weiner WJ

更新日期：1992-01-01 00:00:00

abstract：:The cleavage-product of amyloid precursor protein (APP) constitutes the core component of plaques found in the brains of Alzheimer's disease (AD) patients. APP is ubiquitously expressed and its precise physiological functions remain unclear. This protein has been proposed to regulate synaptic function and processes un...

journal_title：Journal of neurochemistry

authors： Senechal Y,Kelly PH,Cryan JF,Natt F,Dev KK

更新日期：2007-09-01 00:00:00

abstract：:We used both the polymerase chain reaction (PCR) and in situ hybridization to search for the presence of proteolipid protein (PLP) gene transcripts in the developing mouse. Total brain RNA extracted from 13-19-day embryos, analyzed by PCR, demonstrated the presence of a single transcript that was unambiguously identif...

journal_title：Journal of neurochemistry

authors： Timsit SG,Bally-Cuif L,Colman DR,Zalc B

更新日期：1992-03-01 00:00:00

abstract：:Microtubule-associated proteins (MAPs) were phosphorylated by a Ca2+- and calmodulin-dependent protein kinase from rat brain cytosol. The maximal amount of phosphate incorporated into MAPs was 25 nmol of phosphate/mg protein. A Ka value of the enzyme for calmodulin was 57.0 nM, with MAPs as substrates. Among MAPs, MAP...

journal_title：Journal of neurochemistry

authors： Yamamoto H,Fukunaga K,Tanaka E,Miyamoto E

更新日期：1983-10-01 00:00:00

abstract：:We investigated [(18)F]fluoroethyl-harmol ([(18)F]FEH) as a reversible and selective ligand for positron emission tomography (PET) studies of monoamine oxidase A (MAO-A). Binding of [(18)F]FEH in rat brain cryostat sections indicated high affinity (KD = 3 nM), and density (Bmax; 600 pmol/g). The plasma free fraction w...

journal_title：Journal of neurochemistry

authors： Maschauer S,Haller A,Riss PJ,Kuwert T,Prante O,Cumming P

更新日期：2015-12-01 00:00:00

abstract：:Spinocerebellar ataxia 1 (SCA1) is a dominantly inherited neurodegenerative disease associated with progressive ataxia resulting from the loss of cerebellar Purkinje cells (PCs) and neurons in the brainstem. In PCs of SCA1 transgenic mice, the disease causing ataxin-1 protein mediates the formation of S100B containing...

journal_title：Journal of neurochemistry

authors： Hearst SM,Lopez ME,Shao Q,Liu Y,Vig PJ

更新日期：2010-08-01 00:00:00

abstract：:This Editorial highlights an ingenious study by Li and Freeman published in this issue of J. Neurochem. Accurately knowing the brain extracellular levels of the two main substrates, that is, glucose and lactate, while a well-controlled and graded-stimulus is applied, might be extremely useful to finely understand how ...

journal_title：Journal of neurochemistry

authors： Leloup C

更新日期：2015-11-01 00:00:00

abstract：:Glycine residues can introduce flexibility in proteins, give rise to turns and breaks in secondary structure and are key components of some nucleotide binding motifs. In the P2X receptor extracellular ATP binding domain, 11 glycine residues are completely conserved and an additional five are conserved in at least five...

journal_title：Journal of neurochemistry

authors： Digby HR,Roberts JA,Sutcliffe MJ,Evans RJ

更新日期：2005-12-01 00:00:00

abstract：:Retinal ganglion cells transmit the visual signal from the retina to the brain. We have previously shown that the activator protein 2 (AP-2)δ (TFAP2D) transcription factor is expressed in one third of ganglion cells in developing retina suggesting a specialized role for these AP-2δ-expressing cells. Here, we address t...

journal_title：Journal of neurochemistry

authors： Li X,Monckton EA,Godbout R

更新日期：2014-04-01 00:00:00

abstract：:Tanycytes are glial cells in the hypothalamus that are functionally part of the blood-brain barrier. They can sense nutrients and metabolites in the circulation such as glucose, then signal to neuronal systems to influence ingestive behaviour and energy storage, and ultimately affect body weight. The complex structure...

journal_title：Journal of neurochemistry

authors： Ebling FJP

更新日期：2020-11-09 00:00:00

abstract：:Following a previous report on detection of muscarinic receptors in myelin with the implied presence of G proteins, we now demonstrate by more direct means the presence of such proteins and their quantification. Using [35S]guanosine 5'-O-(3-thiotriphosphate) ([35S]GTP gamma S) as the binding ligand, purified myelin fr...

journal_title：Journal of neurochemistry

authors： Larocca JN,Golly F,Ledeen RW

更新日期：1991-07-01 00:00:00

abstract：:The levels of hexokinase, as well as those of the cytoplasmic glycolytic enzyme lactate dehydrogenase and the mitochondrial tricarboxylic acid cycle enzymes fumarase and citrate synthase, have been determined in whole rat brain and in neuronal, astrocytic, and oligodendroglial fractions isolated from rat brain. Compar...

journal_title：Journal of neurochemistry

authors： Snyder CD,Wilson JE

更新日期：1983-04-01 00:00:00

abstract：:To assess the postmortem stability of brain 3-methoxy-4-hydroxy-phenylethyleneglycol (MHPG) and 3,4-dihydroxyphenylethyleneglycol (DHPG) levels, groups of rats and mice were killed by cervical dislocation and left at either 21 degrees or 4 degrees C for intervals of up to 24 h until removal and freezing of whole brain...

journal_title：Journal of neurochemistry

authors： Warsh JJ,Godse DD,Li PP,Cheung SW

更新日期：1981-03-01 00:00:00

abstract：:The subcellular distribution of peptide histidine isoleucine amide (PHI)-27-like peptides (PLP) was investigated in rat cerebral cortex and whole rat brain in comparison with the distribution of vasoactive intestinal peptide (VIP). The highest content of PLP was found in the crude mitochondrial fraction (P2) and was a...

journal_title：Journal of neurochemistry

authors： Korchak DM,Gysling K,Beinfeld MC

更新日期：1985-01-01 00:00:00

abstract：:Dopaminergic neurons in cultures of dissociated cells from fetal rat mesencephalon were exposed to the principal metabolite of the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), 1-methyl-4-phenyl-pyridinium ion (MPP+), and several of its structural analogues. At concentrations between 0.01 and 0.1 mic...

journal_title：Journal of neurochemistry

authors： Sanchez-Ramos JR,Michel P,Weiner WJ,Hefti F

更新日期：1988-06-01 00:00:00

abstract：:Fever is thought to be mediated by leukocytic pyrogen (LP), a polypeptide synthesized by phagocytic leukocytes and which is responsible for the upwards resetting of the hypothalamic thermostat. In an attempt to study the effects of LP directly on brain tissue, purified human LP was incubated with rabbit brain slices i...

journal_title：Journal of neurochemistry

authors： Dinarello CA,Bernheim HA

更新日期：1981-09-01 00:00:00

abstract：:The effects of treatment of brain membranes with diethyl pyrocarbonate (DEP), a histidine-modifying reagent, on the binding of 3H-labeled Ro 15-4513 (ethyl-8-azido-5,6-dihydro-5-methyl-6-oxo-4H-imidazo[1,5-a]- [1,4]benzodiazepine-3-carboxylate) and [3H]diazepam were compared. DEP pretreatment produced a dose-dependent...

journal_title：Journal of neurochemistry

authors： Uusi-Oukari M

更新日期：1992-08-01 00:00:00

abstract：:5-Hydroxytryptamine (5-HT) specifically and reversibly inhibits nicotine-induced currents and catecholamine release in bovine adrenal chromaffin cells in culture. Pharmacological analysis indicates that the inhibition is not mediated by known 5-HT receptor subtypes. The inhibition is noncompetitive over a range of nic...

journal_title：Journal of neurochemistry

authors： Vijayaraghavan S,Schmid HA,Mapp KS

更新日期：1993-07-01 00:00:00

abstract：:Previous work in this laboratory has shown that adrenal medullary transplants into the spinal cord subarachnoid space can reduce pain sensitivity. This analgesia most likely results from the release of neuroactive substances, particularly catecholamines and opioid peptides, from the transplanted cells into the CSF of ...

journal_title：Journal of neurochemistry

authors： Sagen J,Kemmler JE,Wang H

更新日期：1991-02-01 00:00:00

abstract：:Muscarinic receptors are the predominant cholinergic receptors in the central and peripheral nervous systems. Recently, activation of muscarinic receptors was found to elicit pigment granule dispersion in retinal pigment epithelium isolated from bluegill fish. Pigment granule movement in retinal pigment epithelium is ...

journal_title：Journal of neurochemistry

authors： Phatarpekar PV,Durdan SF,Copeland CM,Crittenden EL,Neece JD,García DM

更新日期：2005-12-01 00:00:00

abstract：:In the human fetus, obtained postmortem at estimated gestational ages of 8-22 weeks, biochemical activities of cortical choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) were comparable to those of adult brain tissue. In contrast cholinergic receptor binding, including muscarinic M1 and M2 subtypes (mea...

journal_title：Journal of neurochemistry

authors： Perry EK,Smith CJ,Atack JR,Candy JM,Johnson M,Perry RH

更新日期：1986-10-01 00:00:00

abstract：:A great body of evidence points toward a functional interaction between metabotropic glutamate 5 receptors (mGluR5) and NMDA receptors (NMDAR) that enhances synaptic plasticity and cognition. However, the molecular mechanism underlying this interaction remains unclear. Here, we show that co-activation of mGluR5 and NM...

journal_title：Journal of neurochemistry

authors： Sarantis K,Tsiamaki E,Kouvaros S,Papatheodoropoulos C,Angelatou F

更新日期：2015-11-01 00:00:00

abstract：:Prostaglandin E1 (PGE1)-mediated transmembrane signal control systems were investigated in intact murine neuroblastoma cells (clone N1E-115). PGE1 increased intracellular levels of total inositol phosphates (IP), cyclic GMP, cyclic AMP, and calcium ([Ca2+]i). PGE1 transiently increased inositol 1,4,5-trisphosphate for...

journal_title：Journal of neurochemistry

authors： Kanba S,Sasakawa N,Nakaki T,Kanba KS,Yagi G,Kato R,Richelson E

更新日期：1991-12-01 00:00:00

abstract：:Rabbit retinas were maintained in a physiological state in vitro and exposed to 0.5-h pulses of labeled leucine. Protein synthesis was determined from incorporation of the label, and degradation from its subsequent release. The retinas were treated as test-control pairs. The control retina remained in darkness while t...

journal_title：Journal of neurochemistry

authors： Ames A 3rd,Parks JM,Nesbett FB

更新日期：1980-07-01 00:00:00