Abstract：
:Retinal ganglion cells transmit the visual signal from the retina to the brain. We have previously shown that the activator protein 2 (AP-2)δ (TFAP2D) transcription factor is expressed in one third of ganglion cells in developing retina suggesting a specialized role for these AP-2δ-expressing cells. Here, we address the role of AP-2δ in retina by in ovo electroporation of RCAS/AP-2δ retroviral constructs into the eyes of chick embryos at day 2 of gestation. Ectopic expression of AP-2δ does not affect lineage differentiation in the developing retina. However, immunostaining of retinal tissue with markers associated with axonal growth such as growth-associated protein 43 and polysialic acid-neural cell adhesion molecule (PSA-NCAM) demonstrates axonal misrouting and abnormal axonal bundling. Treatment of AP-2δ-misexpressing retinal cell cultures with endoneuraminidase, an enzyme that removes PSA from NCAM, decreases AP-2δ-induced axonal bundling. Our data suggest a role for AP-2δ in polysialylation of NCAM, with ectopic expression of AP-2δ resulting in premature bundling of emerging axons and misrouting of axons. We propose that expression of AP-2δ in a subset of ganglion cells contributes to the fine-tuning of axonal growth in the developing retina.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Li X,Monckton EA,Godbout Rdoi
10.1111/jnc.12521subject
Has Abstractpub_date
2014-04-01 00:00:00pages
72-84issue
1eissn
0022-3042issn
1471-4159journal_volume
129pub_type
杂志文章abstract：:Proteoglycans (PGs) are major constituents of the extracellular matrix and have recently been proposed to contribute to synaptic plasticity. Hippocampal PGs have not yet been studied or linked to memory. The aim of the study, therefore, was to isolate and characterize rat hippocampal PGs and determine their possible r...
journal_title：Journal of neurochemistry
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journal_title：Journal of neurochemistry
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：1984-10-01 00:00:00
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pub_type： 杂志文章
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1111/j.1471-4159.1989.tb11772.x
更新日期：1989-09-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1983.tb08041.x
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pub_type： 杂志文章
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更新日期：2006-04-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章,评审
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更新日期：2012-01-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1046/j.1471-4159.1996.66041468.x
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更新日期：1998-01-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：1999-10-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章,评审
doi：10.1111/jnc.15035
更新日期：2020-05-07 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1987.tb05686.x
更新日期：1987-05-01 00:00:00
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journal_title：Journal of neurochemistry
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pub_type： 杂志文章
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更新日期：1994-07-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：1990-04-01 00:00:00
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journal_title：Journal of neurochemistry
pub_type： 杂志文章
doi：10.1111/j.1471-4159.1993.tb05852.x
更新日期：1993-01-01 00:00:00
abstract：:P25alpha is a protein normally expressed in oligodendrocytes and subcellular relocalization of p25alpha occurs in multiple system atrophy, Parkinson's disease and Lewy body dementia along with ectopic expression in neurons. Moreover, it accumulates in Lewy body inclusions with aggregated alpha-synuclein and is a poten...
journal_title：Journal of neurochemistry
pub_type： 杂志文章
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更新日期：2008-07-01 00:00:00
