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Separation of neuropeptides by HPLC: evaluation of different supports, with analytical and preparative applications to human and porcine neurophysins, beta-lipotropin, adrenocorticotropic hormone, and beta-endorphin.

Abstract：

:The separation of 38 hypophyseal or hypothalamic peptides and proteins by reversed-phase HPLC is described. The efficacy of several supports is discussed: large-pore reversed-phase silica supports turned out to be most effective in the separation of neuropeptides and proteins. The use of high sodium phosphate and phosphoric acid in the eluent resulted in high yields and better separation. On the basis of this procedure, beta-lipotropin, beta-endorphin, and adrenocorticotropic hormone were prepared from porcine pituitary glands, and human neurophysins from human pituitary posterior lobes. Moreover, this system is used for the analysis of the perifusate of pituitary posterior lobes, showing the release of micro-heterogeneous neurophysins.

journal_name

J Neurochem

journal_title

Journal of neurochemistry

authors

Richter WO,Schwandt P

doi

10.1111/j.1471-4159.1985.tb07156.x

subject

Has Abstract

pub_date

1985-06-01 00:00:00

pages

1697-703

issue

6

eissn

0022-3042

issn

1471-4159

journal_volume

44

pub_type

杂志文章

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