Role of external and internal calcium on heterocarrier-mediated transmitter release.


:Release-regulating heterocarriers exist on brain nerve endings. We have investigated in this study the mechanisms involved in the neurotransmitter release evoked by GABA heterocarrier activation. GABA increased the basal release of [3H]acetylcholine and [3H]noradrenaline from rat hippocampal synaptosomes and of [3H]dopamine from striatal synaptosomes. These GABA effects, insensitive to GABA receptor antagonists, were prevented by inhibiting GABA uptake but not by blocking noradrenaline, choline, or dopamine transport. Lack of extracellular Ca2+ or addition of tetrodotoxin selectively abolished the GABA-evoked release of [3H]noradrenaline, leaving unaffected that of [3H]acetylcholine or [3H]dopamine. 1,2-Bis(2-aminophenoxyl)-ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester (BAPTA-AM) or vesamicol attenuated the release of [3H]acetylcholine elicited by GABA. Reserpine, but not BAPTA-AM, prevented the effect of GABA on [3H] dopamine release. Autoreceptor activation inhibited the GABA-evoked release of [3H]noradrenaline but not that of [3H]acetylcholine or [3H]dopamine. It is concluded that (a) the release of [3H]noradrenaline consequent to activation of GABA heterocarriers sited on noradrenergic terminals meets the criteria of a conventional exocytotic process, (b) the extracellular [Ca2+]-independent releases of [3H]acetylcholine and [3H]dopamine appear to occur from vesicles possibly through involvement of intraterminal Ca2+, and (c) autoreceptor activation only affects heterocarrier-mediated vesicular release linked to entry of extracellular Ca2+.


J Neurochem


Fassio A,Bonanno G,Fontana G,Usai C,Marchi M,Raiteri M




Has Abstract


1996-04-01 00:00:00












  • Modulation of NMDA receptor function by cyclic AMP in cerebellar neurones in culture.

    abstract::The signal transduction pathways involved in NMDA receptor modulation by other receptors remain unclear. cAMP could be involved in this modulation. The aim of this work was to analyse the contribution of cAMP to NMDA receptor modulation in cerebellar neurones in culture. Forskolin increases cAMP and results in increas...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Llansola M,Sánchez-Pérez AM,Montoliu C,Felipo V

    更新日期:2004-11-01 00:00:00

  • The relationship between plasma and brain quinolinic acid levels and the severity of hepatic encephalopathy in animal models of fulminant hepatic failure.

    abstract::Quinolinic acid is an excitatory, neurotoxic tryptophan metabolite proposed to play a role in the pathogenesis of hepatic encephalopathy. This involvement was investigated in rat and rabbit models of fulminant hepatic failure at different stages of hepatic encephalopathy. Although plasma and brain tryptophan levels we...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Basile AS,Saito K,Li Y,Heyes MP

    更新日期:1995-06-01 00:00:00

  • alpha-Synuclein forms a complex with transcription factor Elk-1.

    abstract::alpha-Synuclein has been identified as a component of Lewy bodies in Parkinson's disease and diffuse Lewy body disease, and glial cytoplasmic inclusions (GCIs) in multiple system atrophy (MSA). To explore the role of alpha-synuclein in the pathogenesis, we searched for molecules interacting with alpha-synuclein and di...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Iwata A,Miura S,Kanazawa I,Sawada M,Nukina N

    更新日期:2001-04-01 00:00:00

  • Differential interaction of 1-methyl-4-phenylpyridinium ion with the putatively vesicular binding site of [3H]tyramine in dopaminergic and nondopaminergic brain regions.

    abstract::The neurotoxin 1-methyl-4-phenylpyridinium ion (MPP+) in the brain striatum has recently been shown to bind at a putatively vesicular site labeled by [3H]tyramine ([3H]TY). Whereas in the rat and mouse striatum MPP+ antagonized TY binding competitively, in the cerebellum there was a mixed-type antagonism, which sugges...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Vaccari A,Saba PL,Gessa GL,Del Zompo M

    更新日期:1993-02-01 00:00:00

  • Increased AP-1 DNA binding activity in PC12 cells treated with lead.

    abstract::The possibility that the mechanism of lead neurotoxicity may be at the level of transcription was investigated in PC12 cells. In electrophoretic mobility gel shift assays Pb2+ was found to increase activator protein-1 complex (AP-1) DNA binding activity in PC12 cells; the increase was time- and concentration-dependent...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Chakraborti T,Kim KA,Goldstein GG,Bressler JP

    更新日期:1999-07-01 00:00:00

  • Interactions between vasoactive intestinal peptide and dopamine in the rabbit retina: stimulation of a common adenylate cyclase.

    abstract::Although 3,4-dihydroxyphenylethylamine (dopamine, DA) and vasoactive intestinal peptide (VIP) have been reported to stimulate adenylate cyclase activity in the rabbit retina, possible interactions between VIP-sensitive and DA-sensitive adenylate cyclase systems have not been previously investigated. To elucidate the i...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Pachter JA,Lam DM

    更新日期:1986-01-01 00:00:00

  • Insulin-like growth factor binding protein-1 activates integrin-mediated intracellular signaling and migration in oligodendrocytes.

    abstract::In multiple sclerosis (MS), oligodendrocytes in lesions are lost, leaving damaged tissue virtually devoid of these myelin-producing cells. Our group has recently demonstrated enhanced expression of insulin-like growth factor (IGF) binding protein-1 (IGFBP-1) in oligodendrocytes (CNPase(+)) localized adjacent to MS les...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Chesik D,De Keyser J,Bron R,Fuhler GM

    更新日期:2010-06-01 00:00:00

  • Hydrophobic protein that copurifies with human brain acetylcholinesterase: amino acid sequence, genomic organization, and chromosomal localization.

    abstract::The mechanism of attachment of acetylcholinesterase (AChE) to neuronal membranes in interneuronal synapses is poorly understood. We have isolated, sequenced, and cloned a hydrophobic protein that copurifies with AChE from human caudate nucleus and that we propose forms a part of a complex of membrane proteins attached...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Navaratnam DS,Fernando FS,Priddle JD,Giles K,Clegg SM,Pappin DJ,Craig I,Smith AD

    更新日期:2000-05-01 00:00:00

  • Activation of (Na+, K+)-ATPase by nanomolar concentrations of GM1 ganglioside.

    abstract::GM1 ganglioside binding to the crude mitochondrial fraction of rat brain and its effect on (Na+, K+)-ATPase were studied, the following results being obtained: (a) the binding process followed a biphasic kinetics with a break at 50 nM-GM1; GM1 at concentrations below the break was stably associated, while over the bre...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Leon A,Facci L,Toffano G,Sonnino S,Tettamanti G

    更新日期:1981-08-01 00:00:00

  • A motor neuron strategy to save time and energy in neurodegeneration: adaptive protein stoichiometry.

    abstract::Neurofilament proteins (Nf) are a biomarker of disease progression in amyotrophic lateral sclerosis (ALS). This study investigated whether there are major differences in expression from in vivo measurements of neurofilament isoforms, from the light chain, NfL (68 kDa), compared with larger proteins, the medium chain (...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Zucchi E,Lu CH,Cho Y,Chang R,Adiutori R,Zubiri I,Ceroni M,Cereda C,Pansarasa O,Greensmith L,Malaspina A,Petzold A

    更新日期:2018-09-01 00:00:00

  • Selective destruction of cultured dopaminergic neurons from fetal rat mesencephalon by 1-methyl-4-phenylpyridinium: cytochemical and morphological evidence.

    abstract::Dopaminergic neurons in cultures of dissociated cells from fetal rat mesencephalon were exposed to the principal metabolite of the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP), 1-methyl-4-phenyl-pyridinium ion (MPP+), and several of its structural analogues. At concentrations between 0.01 and 0.1 mic...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Sanchez-Ramos JR,Michel P,Weiner WJ,Hefti F

    更新日期:1988-06-01 00:00:00

  • Mammalian forebrain ketimine reductase identified as μ-crystallin; potential regulation by thyroid hormones.

    abstract::Ketimine reductase (E.C. was purified to apparent homogeneity from lamb forebrain by means of a rapid multi-step chromatography protocol. The purified enzyme was identified by MS/MS (mass spectrometry) as μ-crystallin. The identity was confirmed by heterologously expressing human μ-crystallin in Escherichia ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hallen A,Cooper AJ,Jamie JF,Haynes PA,Willows RD

    更新日期:2011-08-01 00:00:00

  • Brain ion and amino acid contents during edema development in hepatic encephalopathy.

    abstract::Brain edema in hepatic encephalopathy has been associated with circulating ammonia that is metabolized to glutamine. We measured alterations in blood chemistry and brain regional specific gravity and ion and amino acid contents in models of simple hyperammonemia and liver failure induced by daily administrations of am...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hilgier W,Olson JE

    更新日期:1994-01-01 00:00:00

  • Rat brain mast cells: contribution to brain histamine levels.

    abstract::Recent studies have shown that mast cells (MCs) are present in rat brain, that they have a predominantly thalamic localization, and that they contain histamine (HA). However, the degree to which these cells contribute to brain HA levels has remained unclear. Our recent studies of the precise distribution of rat brain ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Goldschmidt RC,Hough LB,Glick SD

    更新日期:1985-06-01 00:00:00

  • The P2X7 receptor links mechanical strain to cytokine IL-6 up-regulation and release in neurons and astrocytes.

    abstract::Mechanical strain in neural tissues can lead to the up-regulation and release of multiple cytokines including interleukin 6 (IL-6). In the retina, the mechanosensitive release of ATP can autostimulate P2X7 receptors on both retinal ganglion cell neurons and optic nerve head astrocytes. Here, we asked whether the purin...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lu W,Albalawi F,Beckel JM,Lim JC,Laties AM,Mitchell CH

    更新日期:2017-05-01 00:00:00

  • RCAN1 regulates vesicle recycling and quantal release kinetics via effects on calcineurin activity.

    abstract::We have previously shown that Regulator of Calcineurin 1 (RCAN1) regulates multiple stages of vesicle exocytosis. However, the mechanisms by which RCAN1 affects secretory vesicle exocytosis and quantal release kinetics remain unknown. Here, we use carbon fibre amperometry to detect exocytosis from chromaffin cells and...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Zanin MP,Mackenzie KD,Peiris H,Pritchard MA,Keating DJ

    更新日期:2013-02-01 00:00:00

  • 5-Methoxytryptamine inhibits cyclic AMP accumulation in cultured retinal neurons through activation of a pertussis toxin-sensitive site distinct from the 2-[125I]iodomelatonin binding site.

    abstract::Melatonin and 5-methoxytryptamine inhibited forskolin-stimulated cyclic AMP formation in cultured neural cells prepared from embryonic chick retina. Both methoxyindoles exhibited similar potency and efficacy, with EC50 values of 0.8 nM for melatonin and 7.2 nM for 5-methoxytryptamine. Inhibition of cyclic AMP formatio...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Iuvone PM,Gan J,Alonso-Gómez AL

    更新日期:1995-04-01 00:00:00

  • Cannabinoid 1 receptor-dependent transactivation of fibroblast growth factor receptor 1 emanates from lipid rafts and amplifies extracellular signal-regulated kinase 1/2 activation in embryonic cortical neurons.

    abstract::G-protein coupled receptors may mediate their effects on neuronal growth and differentiation through activation of extracellular signal-regulated kinases 1/2 (ERK1/2), often elicited by transactivation of growth factor receptor tyrosine kinases. This elaborate signaling process includes inducible formation and traffic...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Asimaki O,Leondaritis G,Lois G,Sakellaridis N,Mangoura D

    更新日期:2011-03-01 00:00:00

  • Characterization of two [3H]glutamate binding sites in rat hippocampal membranes.

    abstract::The specific binding of L-[3H]glutamate was investigated in the presence and the absence of sodium ions in freshly prepared membranes from rat hippocampus. Sodium ions were found to have a biphasic effect; low concentrations induced a marked inhibition of the binding (in the range 0.5-5.0 mM), whereas higher concentra...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Baudry M,Lynch G

    更新日期:1981-03-01 00:00:00

  • Degeneration of cultured cortical neurons following prolonged inactivation: molecular mechanisms.

    abstract::Networks of neurons express persistent spontaneous network activity when maintained in dissociated cultures. Prolonged blockade of the spontaneous activity with tetrodotoxin (TTX) causes the eventual death of the neurons. In this study, we investigated some molecular mechanisms that may underlie the activity-suppresse...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Schonfeld-Dado E,Fishbein I,Segal M

    更新日期:2009-08-01 00:00:00

  • Validation of the targeted metabolomic pathway in the hippocampus and comparative analysis with the prefrontal cortex of social defeat model mice.

    abstract::The proportion of major depressive disorder (MDD) patients around the world has increased remarkably. Although many studies of MDD have been conducted based on classic hypotheses, like alteration of the hypothalamic-pituitary-adrenal axis or monoamine neurotransmitters, the mechanisms underlying MDD remain unclear. Ai...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Xu K,He Y,Chen X,Tian Y,Cheng K,Zhang L,Wang Y,Yang D,Wang H,Wu Z,Li Y,Lan T,Dong Z,Xie P

    更新日期:2019-06-01 00:00:00

  • Synthesis and translocation of gangliosides and glycoproteins during urethane anesthesia.

    abstract::In this work, we have studied (a) the contents of gangliosides, glycoproteins, and phospholipids of the vesicle and plasma membrane fractions from brains of anesthetized and control rats and chickens and (b) the labeling of gangliosides and glycoproteins in the retina ganglion cell layer and optic tectum of urethane-a...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Domowicz MS,Kivatinitz SC,Caputto BL,Caputto R

    更新日期:1988-05-01 00:00:00

  • Activation of the extracellular signal-regulated kinase pathway contributes to the behavioral deficit of fragile x-syndrome.

    abstract::Fragile X syndrome (FXS) is a developmental disorder caused by the loss of Fragile X Mental Retardation 1 (FMR1) gene function because of a CGG repeat expansion (> 200 repeats) in the gene. The molecular mechanism(s) linking loss of FMR1 function to the molecular pathology and cognitive/behavioral disability remain un...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Wang X,Snape M,Klann E,Stone JG,Singh A,Petersen RB,Castellani RJ,Casadesus G,Smith MA,Zhu X

    更新日期:2012-05-01 00:00:00

  • Choline acetyltransferase activity and total protein content in selected optic areas of the rat after complete light-deprivation during CNS development.

    abstract::Choline acetyltransferase (acetyl-CoA: choline O-acetyltransferase, I.U.B. activity and total protein content in visual and extra-visual areas were compared in normal Long-Evans rats and in rats subjected to complete light-deprivation for 21 days from birth. The enzyme activity and the protein content in the ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Maletta GJ,Timiras PS

    更新日期:1968-08-01 00:00:00

  • Release of previously incorporated gamma-[3H]aminobutyric acid in rabbit caudate nucleus slices.

    abstract::The release of gamma-aminobutyric acid (GABA) was studied in slices of the head of the rabbit caudate nucleus. The slices were preincubated with [3H]GABA and then superfused. Aminooxyacetic acid was present throughout. Both the tritium in the slices and that in the superfusate consisted practically entirely of [3H]GAB...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Limberger N,Späth L,Starke K

    更新日期:1986-04-01 00:00:00

  • Cerebral vulnerability is associated with selective increase in extracellular glutamate concentration in experimental thiamine deficiency.

    abstract::Microdialysis in the awake, freely moving rat was used to determine the effect of pyrithiamine-induced thiamine deficiency on the levels of amino acids in the brain. Studies were carried out on (a) presymptomatic animals immediately before the development of behavioral changes and (b) acute symptomatic animals within ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Hazell AS,Butterworth RF,Hakim AM

    更新日期:1993-09-01 00:00:00

  • Nuclear sialyl cholesterol causes changes in the structure of chromatin and its transcription level followed by the promotion of neuritogenesis.

    abstract::Exogenously added sialyl cholesterol (SC) induced neuritogenesis and accumulated in the nuclear fraction of a mouse neuroblastoma cell line, Neuro2a, as reported elsewhere. To clarify the significance of nuclear SC, we examined the structure of chromatin and its transcriptional level. The chromatin of SC-treated cells...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Yamashita T,Tsuji S

    更新日期:1992-04-01 00:00:00

  • Protein phosphotyrosine in mouse brain: developmental changes and regulation by epidermal growth factor, type I insulin-like growth factor, and insulin.

    abstract::Using antiphosphotyrosine antibodies, we have investigated protein phosphorylation in mouse brain during development in intact animals and in reaggregated cerebral cultures. Under basal conditions, in vivo and in vitro, the levels of two main phosphoproteins, of Mr 120,000 and 180,000 (pp180), increased with developme...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Girault JA,Chamak B,Bertuzzi G,Tixier H,Wang JK,Pang DT,Greengard P

    更新日期:1992-02-01 00:00:00

  • A presenilin-independent aspartyl protease prefers the gamma-42 site cleavage.

    abstract::beta-Amyloid peptides (Abeta40 and Abeta42) are the major constituents of amyloid plaques, which are one of the hallmarks of Alzheimer's disease (AD). The Abeta is derived from sequential cleavages of amyloid precursor protein (APP) by beta- and gamma-secretases. gamma-Secretase consists of at least four proteins wher...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lai MT,Crouthamel MC,DiMuzio J,Pietrak BL,Donoviel DB,Bernstein A,Gardell SJ,Li YM,Hazuda D

    更新日期:2006-01-01 00:00:00

  • Expression of facilitative glucose transporter isoforms in human brain tumors.

    abstract::The expression of facilitative glucose transporter (GLUT) isoforms in human astrocytic tumors was examined. Reverse transcriptase-polymerase chain reaction of a surgically biopsied glioblastoma was carried out using the degenerative oligonucleotide primers corresponding to the sequences of the human facilitative gluco...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Nagamatsu S,Sawa H,Wakizaka A,Hoshino T

    更新日期:1993-12-01 00:00:00