A Ca2+-activated channel from Xenopus laevis oocyte membranes reconstituted into planar bilayers.

abstract：:Deficiency in PTEN (phosphatase and tensin homolog deleted on chromosome 10) is the underlying cause of PTEN hamartoma tumor syndrome and a wide variety of human cancers. In skin epidermis, we have previously identified an autocrine FGF signaling induced by loss of Pten in keratinocytes. In this study, we demonstrate ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mathew G,Hannan A,Hertzler-Schaefer K,Wang F,Feng GS,Zhong J,Zhao JJ,Downward J,Zhang X

更新日期：2016-11-15 00:00:00

abstract：:A predictive coarse-grained protein force field [associative memory, water-mediated, structure, and energy model for molecular dynamics (AWSEM)-MD] is used to study the energy landscapes and relative stabilities of amyloid-β protein (1-40) in the monomer and all of its oligomeric forms up to an octamer. We find that a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zheng W,Tsai MY,Chen M,Wolynes PG

更新日期：2016-10-18 00:00:00

abstract：:The conjugal transmission of plasmid ColE1 and its derivatives can lead to recA-independent recombination. The recombination is not observed during vegetative growth and takes place specifically between the sequences that are thought to represent the transfer origin of ColE1. This provides genetic evidence for breakag...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Warren GJ,Clark AJ

更新日期：1980-11-01 00:00:00

abstract：:B-DNA becomes unstable under superhelical stress and is able to adopt a wide range of alternative conformations including strand-separated DNA and Z-DNA. Localized sequence-dependent structural transitions are important for the regulation of biological processes such as DNA replication and transcription. To directly p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oberstrass FC,Fernandes LE,Bryant Z

更新日期：2012-04-17 00:00:00

abstract：:In the developing cerebellum, switching of the subunit composition of NMDA receptors occurs in granule cells from NR2B-containing receptors to NR2C-containing ones. We investigated the mechanisms underlying switching of NR2B and NR2C subunit composition in primary cultures of mouse granule cells at the physiological K...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Iijima K,Abe H,Okazawa M,Moriyoshi K,Nakanishi S

更新日期：2008-08-19 00:00:00

abstract：:Members of the platelet-derived growth factor (PDGF) ligand family are known to play important roles in wound healing and fibrotic disease. We show that both transient and stable expression of PDGF-C results in the development of liver fibrosis consisting of the deposition of collagen in a pericellular and perivenular...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Campbell JS,Hughes SD,Gilbertson DG,Palmer TE,Holdren MS,Haran AC,Odell MM,Bauer RL,Ren HP,Haugen HS,Yeh MM,Fausto N

更新日期：2005-03-01 00:00:00

abstract：:The principal axes of inertia are eigenvectors that can be calculated for any rigid body. We report studies of the position of the principal axes in crystallographically solved protein molecules. We find with high frequency that at least one principal axis penetrates the surface of the respective protein in a region u...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Foote J,Raman A

更新日期：2000-02-01 00:00:00

abstract：:A model is presented for age-specific selection on the genotypes in a population. Each genotype is assigned a life table that specifies the viability and fecundity of its age classes. Breeding and reproduction occur at regular intervals, and generations overlap. Examples were generated on a digital computer. Gene freq...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Anderson WW,King CE

更新日期：1970-07-01 00:00:00

abstract：:Clonal expansion of premalignant lesions is an important step in the progression to cancer. This process is commonly considered to be a consequence of sustaining a proliferative mutation. Here, we investigate whether the growth trajectory of clones can be better described by a model in which clone growth does not depe...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chao DL,Eck JT,Brash DE,Maley CC,Luebeck EG

更新日期：2008-09-30 00:00:00

abstract：:Compressional waves that sample the lowermost mantle west of Central America show a rapid change in travel times of up to 4 s over a sampling distance of 300 km and a change in waveforms. The differential travel times of the PKP waves (which traverse Earth's core) correlate remarkably well with predictions for S-wave ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sun X,Song X,Zheng S,Helmberger DV

更新日期：2007-01-02 00:00:00

abstract：:We have cloned and completely sequenced a gene encoding the heavy chain of Dictyostelium myosin I. Like the myosin I molecules from Acanthamoeba, the Dictyostelium myosin I heavy chain is composed of a globular head domain fused to a 45-kDa glycine-, proline-, and alanine-rich carboxyl-terminal domain, rather than the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jung G,Saxe CL 3rd,Kimmel AR,Hammer JA 3rd

更新日期：1989-08-01 00:00:00

abstract：:Limited trypsinization of the fatty acid synthetase multienzyme complex from rat mammary gland results in the release of a protein, molecular weight 32,000, with thioesterase activity. The other components of the multienzyme complex--the acyl carrier protein, acetyl and malonyl transferases, condensing enzyme, keto re...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Smith S,Agradi E,Libertini L,Dileepan KN

更新日期：1976-04-01 00:00:00

abstract：:Expression of the Arabidopsis TRANSPARENT TESTA 2 (TT2) MYB family transcription factor leads to massive accumulation of proanthocyanidins (PAs) in hairy roots of Medicago truncatula. Microarray analysis showed that TT2 induces genes for flavonoid/PA biosynthesis, transcription factors, and a large number of genes of ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pang Y,Peel GJ,Sharma SB,Tang Y,Dixon RA

更新日期：2008-09-16 00:00:00

abstract：:Stable structures of icosahedral symmetry can serve numerous functional roles, including chemical microencapsulation and delivery of drugs and biomolecules, epitope presentation to allow for an efficient immunization process, synthesis of nanoparticles of uniform size, observation of encapsulated reactive intermediate...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Olson AJ,Hu YH,Keinan E

更新日期：2007-12-26 00:00:00

abstract：:Molecular genetic analyses of the young pseudogenes of the immunoglobulin C epsilon genes were carried out to obtain qualitative evidence for the phylogenetic branching pattern of hominoid primates. We found that Old World monkeys had two C epsilon genes, one of which was processed. Among the hominoids examined only t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ueda S,Takenaka O,Honjo T

更新日期：1985-06-01 00:00:00

abstract：:After prolonged visual adaptation of Drosophila, dramatic long-term changes of in vitro phosphorylation of a 50-kDa brain protein that is identical to the Ca2+/calmodulin-dependent protein kinase (EC 2.7.1.37) can be measured in isolated heads. By selective receptor cell desensitization in blue light, subcellular dist...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Willmund R,Mitschulat H,Schneider K

更新日期：1986-12-01 00:00:00

abstract：:Establishing a timeline for the evolution of novelties is a common, unifying goal at the intersection of evolutionary and developmental biology. Analyses of gene regulatory networks (GRNs) provide the ability to understand the underlying genetic and developmental mechanisms responsible for the origin of morphological ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thompson JR,Erkenbrack EM,Hinman VF,McCauley BS,Petsios E,Bottjer DJ

更新日期：2017-06-06 00:00:00

abstract：:In the plant Nicotiana plumbaginifolia, manganese superoxide dismutase (MnSOD) is synthesized in the cytoplasm as a preprotein and is subsequently translocated to the mitochondrial matrix with corresponding cleavage of an NH2-terminal leader sequence. To determine whether the plant enzyme could replace the endogenous ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bowler C,Alliotte T,Van den Bulcke M,Bauw G,Vandekerckhove J,Van Montagu M,Inzé D

更新日期：1989-05-01 00:00:00

abstract：:Peanut or groundnut (Arachis hypogaea L.), a legume of South American origin, has high seed oil content (45-56%) and is a staple crop in semiarid tropical and subtropical regions, partially because of drought tolerance conferred by its geocarpic reproductive strategy. We present a draft genome of the peanut A-genome p...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen X,Li H,Pandey MK,Yang Q,Wang X,Garg V,Li H,Chi X,Doddamani D,Hong Y,Upadhyaya H,Guo H,Khan AW,Zhu F,Zhang X,Pan L,Pierce GJ,Zhou G,Krishnamohan KA,Chen M,Zhong N,Agarwal G,Li S,Chitikineni A,Zhang GQ

更新日期：2016-06-14 00:00:00

abstract：:A recently described experimental system for analyzing the mode of action of a morphogen gradient involves the in situ hybridization of sectioned tissue constructs. In these constructs, a source of activin signaling induces the transcription of several mesodermal genes in blastula animal caps, according to the positio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gurdon JB,Mitchell A,Ryan K

更新日期：1996-09-03 00:00:00

abstract：:We describe a fractionation and purification scheme for the Drosophila RNA polymerase II general transcription factors. Drosophila TFIIE, TFIIF, TFIIH, and RNA polymerase II have been purified to greater than 50% homogeneity from Drosophila embryo nuclear extracts. TFIID has been purified 80-fold and is not significan...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Austin RJ,Biggin MD

更新日期：1996-06-11 00:00:00

abstract：:We present a mechanism by which organisms with only a single photoreceptor, which have a monochromatic view of the world, can achieve color discrimination. An off-axis pupil and the principle of chromatic aberration (where different wavelengths come to focus at different distances behind a lens) can combine to provide...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Stubbs AL,Stubbs CW

更新日期：2016-07-19 00:00:00

abstract：:Most, if not all, peptide- and protein-based hydrogels formed by self-assembly can be characterized as kinetically trapped 3D networks of fibrils. The propensity of disease-associated amyloid-forming peptides and proteins to assemble into polymorphic fibrils suggests that cross-β fibrils comprising hydrogels may also ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nagy-Smith K,Moore E,Schneider J,Tycko R

更新日期：2015-08-11 00:00:00

abstract：:The crystal structure of the murine Fab S-20-4 from a protective anti-cholera Ab specific for the lipopolysaccharide Ag of the Ogawa serotype has been determined in its unliganded form and in complex with synthetic fragments of the Ogawa O-specific polysaccharide (O-SP). The upstream terminal O-SP monosaccharide is sh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Villeneuve S,Souchon H,Riottot MM,Mazie JC,Lei P,Glaudemans CP,Kovác P,Fournier JM,Alzari PM

更新日期：2000-07-18 00:00:00

abstract：:Meiosis-specific homologs of RecA protein have been identified in Saccharomyces cerevisiae and higher eukaryotes including mammals, but their enzymatic activities have not been described. We have purified the human protein HsDmc1 produced in Escherichia coli from a cloned copy of the cDNA. The recombinant enzyme had D...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li Z,Golub EI,Gupta R,Radding CM

更新日期：1997-10-14 00:00:00

abstract：:In a search for Polo-like kinase 1 (Plk1)-interacting proteins using a yeast two-hybrid system, we have identified histone acetyltransferase binding to the origin recognition complex 1 (Hbo1) as a potential Plk1 target. Here, we show that the interaction between Plk1 and Hbo1 is mitosis-specific and that Plk1 phosphor...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wu ZQ,Liu X

更新日期：2008-02-12 00:00:00

abstract：:Murine monoclonal antibodies that bind selectively to adducts formed on DNA by the antitumor drug cis-diamminedichloroplatinum(II), cis-DDP, or to the chemotherapeutically inactive trans isomer trans-DDP were elicited by immunization with calf thymus DNA modified with either cis- or trans-DDP at ratios of bound platin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Sundquist WI,Lippard SJ,Stollar BD

更新日期：1987-12-01 00:00:00

abstract：:The human Bernard-Soulier syndrome is an autosomal recessive disorder of platelet dysfunction presenting with mild thrombocytopenia, circulating "giant" platelets and a bleeding phenotype. The bleeding in patients with the Bernard-Soulier syndrome is disproportionately more severe than suggested by the reduced platele...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Ware J,Russell S,Ruggeri ZM

更新日期：2000-03-14 00:00:00

abstract：:The blind Mexican cave tetra, Astyanax mexicanus, is a unique model system for the study of parallelism and the evolution of cave-adapted traits. Understanding the genetic basis for these traits has recently become feasible thanks to production of a genome-wide linkage map and quantitative trait association analyses. ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gross JB,Protas M,Conrad M,Scheid PE,Vidal O,Jeffery WR,Borowsky R,Tabin CJ

更新日期：2008-12-23 00:00:00

abstract：:The inability to perceive and coordinate both internal and external signals that function to regulate cellular growth and proliferation is a hallmark of oncogenic transformation. To examine the effects of the v-mos oncogene on distinct signal transduction pathways, the 6m2 cell line was used, in which expression of th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Hamilton BJ,DeFranco D

更新日期：1989-01-01 00:00:00