Subcellular localization of 5'-nucleotidase in rat brain.

abstract：:gamma-Aminobutyric acid (GABA) and glutamic acid decarboxylase (GAD) activities were measured in the ovary and the Fallopian tube of rats and compared with brain values. GABA levels in the Fallopian tube were about twice as high as in the brain, while in the ovary they represented only about 5% of the amino acid conte...

journal_title：Journal of neurochemistry

authors： Apud JA,Tappaz ML,Celotti F,Negri-Cesi P,Masotto C,Racagni G

更新日期：1984-07-01 00:00:00

abstract：:DNA sequences encoding two variants of a novel gamma-aminobutyric acidA (GABAA) receptor beta subunit were isolated from an embryonic chicken whole-brain cDNA library and a chicken genomic library. The coding regions of these variants only differ from each other by the absence or presence of 12 bp in the region that e...

journal_title：Journal of neurochemistry

authors： Bateson AN,Lasham A,Darlison MG

更新日期：1991-04-01 00:00:00

abstract：:Recent studies have shown that mast cells (MCs) are present in rat brain, that they have a predominantly thalamic localization, and that they contain histamine (HA). However, the degree to which these cells contribute to brain HA levels has remained unclear. Our recent studies of the precise distribution of rat brain ...

journal_title：Journal of neurochemistry

authors： Goldschmidt RC,Hough LB,Glick SD

更新日期：1985-06-01 00:00:00

abstract：:Enzymes considered to be markers for neurons (angiotensin converting enzyme, thermolysin-like metalloendopeptidase, alanine aminopeptidase, and glutamate-oxaloacetate transaminase), glia (glutamine synthetase, pyruvate carboxylase, and beta-glucuronidase), and endothelial cells (alkaline phosphatase and plasminogen ac...

journal_title：Journal of neurochemistry

authors： Butterworth J

更新日期：1986-08-01 00:00:00

abstract：:Activation of metabotropic glutamate receptors (mGluRs) in glia results in significant physiological effects for both the glia and the neighboring neurons; but in many cases, the mGluR subtypes and signal transduction mechanisms mediating these effects have not been determined. In this study, we report that mGluR acti...

journal_title：Journal of neurochemistry

authors： Peavy RD,Conn PJ

更新日期：1998-08-01 00:00:00

abstract：:The RGS proteins are a recently discovered family of G protein regulators that have been shown to act as GTPase-activating proteins (GAPs) on the G(alpha i) and G(alpha q) subfamilies of the heterotrimeric G proteins. Here, we demonstrate that RGS7 is a potent GAP in vitro on G(alpha i1), and G(alpha o) heterotrimeric...

journal_title：Journal of neurochemistry

authors： Shuey DJ,Betty M,Jones PG,Khawaja XZ,Cockett MI

更新日期：1998-05-01 00:00:00

abstract：:Extracellular dopamine (DA) concentrations estimated by transcerebral dialysis and D1-dependent c-fos expression, as demonstrated by Fos immunohistochemistry, were studied after blockade of DA reuptake by GBR-12909. Rats implanted with dialysis probes in the dorsal caudate-putamen did not show any Fos-positive neurona...

journal_title：Journal of neurochemistry

authors： Morelli M,Carboni E,Cozzolino A,Tanda GL,Pinna A,Di Chiara G

更新日期：1992-09-01 00:00:00

abstract：:The activities of the three major catecholamine-synthesizing enzymes were determined in brain tissue pellets dissected from 500-micrometers thick horizontal sections of rat lower brainstem. The rostrocaudal distributions of the three enzymatic activities were generally not parallel, suggesting differences in the respe...

journal_title：Journal of neurochemistry

authors： Chamba G,Denoroy L,Renaud B

更新日期：1982-08-01 00:00:00

abstract：:Liver fatty acid-binding protein (FABP1, L-FABP) has high affinity for and enhances uptake of arachidonic acid (ARA, C20:4, n-6) which, when esterified to phospholipids, is the requisite precursor for synthesis of endocannabinoids (EC) such as arachidonoylethanolamide (AEA) and 2-arachidonoylglycerol (2-AG). The brain...

journal_title：Journal of neurochemistry

authors： Martin GG,Chung S,Landrock D,Landrock KK,Huang H,Dangott LJ,Peng X,Kaczocha M,Seeger DR,Murphy EJ,Golovko MY,Kier AB,Schroeder F

更新日期：2016-08-01 00:00:00

abstract：:The presence of two heterologous alpha subunits and a single benzodiazepine binding site in the GABA(A) receptor implicates the existence of pharmacologically active and inactive alpha subunits. This fact raises the question of whether a particular alpha subtype could predominate performing the benzodiazepine binding ...

journal_title：Journal of neurochemistry

authors： del Río JC,Araujo F,Ramos B,Ruano D,Vitorica J

更新日期：2001-10-01 00:00:00

abstract：:A new technology called in vivo spectrophotometry was applied to the quantitative determination of the variations in local acetylcholinesterase (AChE) activities. Repeated measurements of the enzyme activities in the same live animal allowed the study of the in vivo inhibition of AChE by amitriptyline. Interactions be...

journal_title：Journal of neurochemistry

authors： Chappey O,Testylier G,Gourmelon P,Galonnier M,Bourre JM,Fatome M,Scherrmann JM,Viret J

更新日期：1990-01-01 00:00:00

abstract：:The mediatophore is a presynaptic membrane protein that has been shown to translocate acetylcholine (ACh) under calcium stimulation when reconstituted into artificial membranes. The mediatophore subunit, a 15-kDa proteolipid, presents a very high sequence homology with the N,N'-dicyclohexylcarbodiimide (DCCD)-binding ...

journal_title：Journal of neurochemistry

authors： Sbia M,Diebler MF,Morel N,Israël M

更新日期：1992-10-01 00:00:00

abstract：:In this article, we demonstrate that an increase in intracellular Ca2+ concentration may represent a specific common step(s) in the mechanism(s) of action of glutamate (Glu) and depolarizing agents on formation of inositol phosphates (IPs) in 8-day-old rat forebrain synaptoneurosomes. In fact, A23187, a Ca2+ ionophore...

journal_title：Journal of neurochemistry

authors： Guiramand J,Vignes M,Récasens M

更新日期：1991-11-01 00:00:00

abstract：:The herpes simplex virus type 2 (HSV-2) protein ICP10PK has anti-apoptotic activity in virus-infected hippocampal cultures through activation of the Ras/Raf-1/MEK/ERK pathway. To exclude the possible contribution of other viral proteins to cell fate determination, we examined the survival of primary hippocampal cultur...

journal_title：Journal of neurochemistry

authors： Wales SQ,Li B,Laing JM,Aurelian L

更新日期：2007-10-01 00:00:00

abstract：:When cultured astroglia are treated with agents that elevate intracellular cyclic AMP, they become process-bearing stellate cells and resemble differentiated astrocytes in vivo. Thrombin rapidly reversed the stellation induced by dibutyryl cyclic AMP, forskolin, or isoproterenol in cultured rat astrocytes; half-maxima...

journal_title：Journal of neurochemistry

authors： Cavanaugh KP,Gurwitz D,Cunningham DD,Bradshaw RA

更新日期：1990-05-01 00:00:00

abstract：:Lactoferrin (Lf) is an iron-binding protein involved in host defense against infection and severe inflammation, which accumulates in the brain during neurodegenerative disorders. Prior to determining Lf function in pathological brain tissues, we investigated its transport through the blood-brain barrier (BBB) in infla...

journal_title：Journal of neurochemistry

authors： Fillebeen C,Dehouck B,Benaïssa M,Dhennin-Duthille I,Cecchelli R,Pierce A

更新日期：1999-12-01 00:00:00

abstract：:Dibutyryl cyclic AMP (dBcAMP) is known to induce maturation and differentiation in astrocytes. As myo-inositol is an important osmoregulator in astrocytes, we examined the effects of maturation and biochemical differentiation on the kinetic properties of myo-inositol transport. Treatment of astrocytes with dBcAMP sign...

journal_title：Journal of neurochemistry

authors： Isaacks RE,Bender AS,Reuben JS,Kim CY,Shi YF,Norenberg MD

更新日期：1999-07-01 00:00:00

abstract：:The neurotransmitter glutamate, once released into the synaptic cleft, is largely recycled by the glutamate-glutamine cycle, which involves uptake into astrocytes, conversion into glutamine and subsequent release of glutamine from astrocytes as a precursor for neuroneal glutamate synthesis. We analysed glutamine efflu...

journal_title：Journal of neurochemistry

authors： Deitmer JW,Bröer A,Bröer S

更新日期：2003-10-01 00:00:00

abstract：:We have examined the catecholamine-sensitive adenylate cyclase in the retina of the white perch (Roccus americanus). Both dopamine and the beta-adrenergic agonist isoproterenol stimulate cyclic AMP accumulation in this retina, but serotonin, an indoleamine, and phenylephrine, an alpha-adrenergic agonist, had no effect...

journal_title：Journal of neurochemistry

authors： O'Connor P,Kropf RB,Dowling JE

更新日期：1989-09-01 00:00:00

abstract：:Gliomas are the most common primary malignant brain tumor in adults, but current treatment for glioblastoma multiforme (GBM) is insufficient. Even though glucose is the primary energetic substrate of glioma cells, they are capable of using fatty acids to generate energy. Fatty acid oxidation (FAO) in mitochondria requ...

journal_title：Journal of neurochemistry

authors： Juraszek B,Czarnecka-Herok J,Nałęcz KA

更新日期：2020-07-12 00:00:00

abstract：:Substantial amounts of phospholipase A2 activity were detected in bovine brain cytosol. The major phospholipase A2 activity was present in the precipitate at 40% saturation with solid ammonium sulfate. After the desaltate of the precipitate was loaded onto an Ultrogel AcA 54 gel filtration column, almost all the activ...

journal_title：Journal of neurochemistry

authors： Hirashima Y,Farooqui AA,Mills JS,Horrocks LA

更新日期：1992-08-01 00:00:00

abstract：:Regulation of muscarinic acetylcholine receptor concentration by receptor activity in neuron-like NG108-15 hybrid cells is a highly specific process. Receptor levels, monitored by binding of [3H]quinuclidinyl benzilate ([3H]QNB), decreased 50--75% following 24-h incubation of cells with muscarinic agonists, but none o...

journal_title：Journal of neurochemistry

authors： Siman RG,Klein WL

更新日期：1981-11-01 00:00:00

abstract：:D-serine is a co-agonist of NMDA receptor (NMDAR) and plays important roles in synaptic plasticity mechanisms. Serine racemase (SR) is a brain-enriched enzyme that converts L-serine to D-serine. SR interacts with the protein interacting with C-kinase 1 (PICK1), which is known to direct protein kinase C (PKC) to its ta...

journal_title：Journal of neurochemistry

authors： Vargas-Lopes C,Madeira C,Kahn SA,Albino do Couto I,Bado P,Houzel JC,De Miranda J,de Freitas MS,Ferreira ST,Panizzutti R

更新日期：2011-01-01 00:00:00

abstract：:Precise cellular targeting of macromolecular cargos has important biotechnological and medical implications. Using a recently established 'protein stapling' method, we linked the proteolytic domain of botulinum neurotoxin type A (BoNT/A) to a selection of ligands to target neuroendocrine tumor cells. The botulinum pro...

journal_title：Journal of neurochemistry

authors： Arsenault J,Ferrari E,Niranjan D,Cuijpers SA,Gu C,Vallis Y,O'Brien J,Davletov B

更新日期：2013-07-01 00:00:00

abstract：:The cleavage-product of amyloid precursor protein (APP) constitutes the core component of plaques found in the brains of Alzheimer's disease (AD) patients. APP is ubiquitously expressed and its precise physiological functions remain unclear. This protein has been proposed to regulate synaptic function and processes un...

journal_title：Journal of neurochemistry

authors： Senechal Y,Kelly PH,Cryan JF,Natt F,Dev KK

更新日期：2007-09-01 00:00:00

abstract：:We have previously shown that acrolein, a lipid peroxidation byproduct, is significantly increased following spinal cord injury in vivo, and that exposure to neuronal cells results in oxidative stress, mitochondrial dysfunction, increased membrane permeability, impaired axonal conductivity, and eventually cell death. ...

journal_title：Journal of neurochemistry

authors： Hamann K,Nehrt G,Ouyang H,Duerstock B,Shi R

更新日期：2008-02-01 00:00:00

abstract：:Stimulated dopamine overflow has been measured with in vivo voltammetry in the caudate-putamen and nucleus accumbens. Overflow was induced by electrical stimulation of the medial forebrain bundle with 120 1-ms, 300-microA, biphasic pulses at frequencies between 10 and 60 Hz. Overflow was measured with a Nafion-coated,...

journal_title：Journal of neurochemistry

authors： May LJ,Wightman RM

更新日期：1989-09-01 00:00:00

abstract：:Cationic amino acids are transported from blood into brain by a saturable carrier at the blood-brain barrier (BBB). The transport properties of this carrier were examined in the rat using an in situ brain perfusion technique. Influx into brain via this system was found to be sodium independent and followed Michaelis-M...

journal_title：Journal of neurochemistry

authors： Stoll J,Wadhwani KC,Smith QR

更新日期：1993-05-01 00:00:00

abstract：:NMDA (N-methyl-D-aspartate) subtype of glutamate receptors are core components of dendritic spine postsynaptic densities (PSDs), in which they are anchored via their carboxy-terminal tails to cytoskeletal proteins. In this study, we examined the role of the neuronal intermediate filament protein, neurofilament-light (...

journal_title：Journal of neurochemistry

authors： Ratnam J,Teichberg VI

更新日期：2005-02-01 00:00:00

abstract：:The ability of cholinergic vesicles to incorporate acetylcholine (ACh) was studied using highly purified synaptic vesicles from Torpedo electric organ. Depleted vesicles were capable of rapidly taking up exogenous ACh. Evidence that this represented true incorporation was that labelled ACh comigrated with vesicular AT...

journal_title：Journal of neurochemistry

authors： Diebler MF,Morot-Gaudry Y

更新日期：1981-08-01 00:00:00