Hydralazine inhibits compression and acrolein-mediated injuries in ex vivo spinal cord.


:We have previously shown that acrolein, a lipid peroxidation byproduct, is significantly increased following spinal cord injury in vivo, and that exposure to neuronal cells results in oxidative stress, mitochondrial dysfunction, increased membrane permeability, impaired axonal conductivity, and eventually cell death. Acrolein thus may be a key player in the pathogenesis of spinal cord injury, where lipid peroxidation is known to be involved. The current study demonstrates that the acrolein scavenger hydralazine protects against not only acrolein-mediated injury, but also compression in guinea pig spinal cord ex vivo. Specifically, hydralazine (500 mumol/L to 1 mmol/L) can significantly alleviate acrolein (100-500 mumol/L)-induced superoxide production, glutathione depletion, mitochondrial dysfunction, loss of membrane integrity, and reduced compound action potential conduction. Additionally, 500 mumol/L hydralazine significantly attenuated compression-mediated membrane disruptions at 2 and 3 h following injury. This was consistent with our findings that acrolein-lys adducts were increased following compression injury ex vivo, an effect that was prevented by hydralazine treatment. These findings provide further evidence for the role of acrolein in spinal cord injury, and suggest that acrolein-scavenging drugs such as hydralazine may represent a novel therapy to effectively reduce oxidative stress in disorders such as spinal cord injury and neurodegenerative diseases, where oxidative stress is known to play a role.


J Neurochem


Hamann K,Nehrt G,Ouyang H,Duerstock B,Shi R




Has Abstract


2008-02-01 00:00:00














  • Estrogen-related receptor gamma regulates dopaminergic neuronal phenotype by activating GSK3β/NFAT signaling in SH-SY5Y cells.

    abstract::The orphan nuclear receptor estrogen-related receptor gamma (ERRγ) is highly expressed in the nervous system during embryogenesis and in adult brains, but its physiological role in neuronal development remains unknown. In this study, we evaluated the relevance of ERRγ in regulating dopaminergic (DAergic) phenotype and...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lim J,Choi HS,Choi HJ

    更新日期:2015-05-01 00:00:00

  • Cell entry strategy of clostridial neurotoxins.

    abstract::Tetanus neurotoxin and botulinum neurotoxins are the causative agents of tetanus and botulism. They block the release of neurotransmitters from synaptic vesicles in susceptible animals and man and act in nanogram quantities because of their ability to specifically attack motoneurons. They developed an ingenious strate...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Binz T,Rummel A

    更新日期:2009-06-01 00:00:00

  • Localisation of the MRC OX-2 glycoprotein on the surfaces of neurones.

    abstract::The MRC OX-2 monoclonal antibody recognises membrane glycoproteins of Mr 41,000 in rat brain and 47,000 on thymocytes. It also reacts with follicular dendritic cells in lymphoid organs, endothelium, smooth muscle, and B-lymphocytes. Indirect immunoperoxidase staining of cryostat sections showed that OX-2 antigen was p...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Webb M,Barclay AN

    更新日期:1984-10-01 00:00:00

  • Inhibition of tyrosine hydroxylase activity by serotonin in explants of newborn rat locus ceruleus.

    abstract::The long-term regulation of tyrosine hydroxylase (TH) by serotonin has been studied with cultures of newborn rat locus ceruleus explants. The presence of serotonin in the culture medium for a 24-h period was followed by an inhibition of TH activity in the explants. This effect lasted several days, with a maximal effec...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Devau G,Multon MF,Pujol JF,Buda M

    更新日期:1987-09-01 00:00:00

  • Characterization of quinolinic acid phosphoribosyltransferase in human blood and observations in Huntington's disease.

    abstract::Quinolinic acid (QUIN), an excitotoxic compound present in the mammalian CNS and periphery, has been hypothetically linked to human neurodegenerative disorders such as Huntington's disease and epilepsy. Quinolinic acid phosphoribosyltransferase (QPRT), the catabolic enzyme of QUIN, is found in the CNS and peripheral o...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Foster AC,Schwarcz R

    更新日期:1985-07-01 00:00:00

  • Glucocorticoid-mediated increases in glycerol phosphate dehydrogenase activity is inhibited by the phorbol ester tumor promoters.

    abstract::An oligodendroglial specific property, glucocorticoid regulation of glycerol-3-phosphate dehydrogenase (GPDH) levels, was inhibited in C6 rat glioma cells when 4 beta-phorbol 12-myristate 13-acetate (PMA) was added to the cultures. PMA inhibited GPDH induction in both logarithmic- and stationary-phase cells. These eve...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Bressler JP,Weingarten D,Kornblith PL

    更新日期:1985-10-01 00:00:00

  • Mitochondrial complex I deficiency in Parkinson's disease.

    abstract::The structure and function of mitochondrial respiratory-chain enzyme proteins were studied postmortem in the substantia nigra of nine patients with Parkinson's disease and nine matched controls. Total protein and mitochondrial mass were similar in the two groups. NADH-ubiquinone reductase (Complex I) and NADH cytochro...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Schapira AH,Cooper JM,Dexter D,Clark JB,Jenner P,Marsden CD

    更新日期:1990-03-01 00:00:00

  • Dkk-3 is elevated in CSF and plasma of Alzheimer's disease patients.

    abstract::Biomarkers in CSF can offer improved diagnostic accuracy for Alzheimer's disease (AD). The present study investigated whether the glycoprotein and putative tumor suppressor Dickkopf homolog 3 (Dkk-3) is secreted into CSF and evaluated its applicability as a diagnostic marker for AD. Using our highly specific immunoenz...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Zenzmaier C,Marksteiner J,Kiefer A,Berger P,Humpel C

    更新日期:2009-07-01 00:00:00

  • Leucine-rich repeat kinase 2 phosphorylation on synapsin I regulates glutamate release at pre-synaptic sites.

    abstract::Leucine-rich repeat kinase 2 (LRRK2) is a large multidomain scaffolding protein with kinase and GTPase activities involved in synaptic vesicle (SV) dynamics. While its role in Parkinson's disease has been largely investigated, little is known about LRRK2 physiological role and until now few proteins have been describe...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Marte A,Russo I,Rebosio C,Valente P,Belluzzi E,Pischedda F,Montani C,Lavarello C,Petretto A,Fedele E,Baldelli P,Benfenati F,Piccoli G,Greggio E,Onofri F

    更新日期:2019-08-01 00:00:00

  • Gemfibrozil, food and drug administration-approved lipid-lowering drug, increases longevity in mouse model of late infantile neuronal ceroid lipofuscinosis.

    abstract::Late Infantile Neuronal Ceroid Lipofuscinosis (LINCL) is a rare neurodegenerative disease caused by mutations in the Cln2 gene that leads to deficiency or loss of function of the tripeptidyl peptidase 1 (TPP1) enzyme. TPP1 deficiency is known to cause the accumulation of autofluoroscent lipid-protein pigments in brain...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ghosh A,Rangasamy SB,Modi KK,Pahan K

    更新日期:2017-05-01 00:00:00

  • Ammonia increases paracellular permeability of rat brain endothelial cells by a mechanism encompassing oxidative/nitrosative stress and activation of matrix metalloproteinases.

    abstract::Ammonia is responsible for cerebral edema associated with acute liver failure, but the role of the vasogenic mechanism has been a matter of dispute. Here, we tested the hypothesis that ammonia induces changes in blood-brain barrier (BBB) permeability by a mechanism coupled to oxidative/nitrosative stress (ONS) evoked ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Skowrońska M,Zielińska M,Wójcik-Stanaszek L,Ruszkiewicz J,Milatovic D,Aschner M,Albrecht J

    更新日期:2012-04-01 00:00:00

  • Glucose transport and utilization are altered in the brain of rats deficient in n-3 polyunsaturated fatty acids.

    abstract::Long-chain polyunsaturated (n-3) fatty acids have been reported to influence the efficiency of membrane receptors, transporters and enzymes. Because the brain is particularly rich in docosahexaenoic acid (DHA, 22:6 n-3), the present study addresses the question of whether the 22:6 n-3 fatty acid deficiency induces dis...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ximenes da Silva A,Lavialle F,Gendrot G,Guesnet P,Alessandri JM,Lavialle M

    更新日期:2002-06-01 00:00:00

  • Increased expression of cholesterol 24S-hydroxylase results in disruption of glial glutamate transporter EAAT2 association with lipid rafts: a potential role in Alzheimer's disease.

    abstract::The glial glutamate transporter EAAT2 (excitatory amino acid transporter 2) is the major mediator of glutamate clearance that terminates glutamate-mediated neurotransmission. Loss of EAAT2 and associated glutamate uptake function has been reported in the brains of patients with Alzheimer's disease (AD). We previously ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Tian G,Kong Q,Lai L,Ray-Chaudhury A,Lin CL

    更新日期:2010-05-01 00:00:00

  • Muscarinic and nicotinic cholinergic binding sites in the terminal abdominal ganglion of the cricket (Acheta domesticus).

    abstract::Cricket (Acheta domesticus) terminal abdominal ganglia (TG) contain high concentrations (approximately 2 pmol/mg protein) of muscarinic and nicotinic cholinergic binding sites, based on the capacity of TG to bind specifically the labelled ligands L-[3H]quinuclidinyl benzilate ([3H]QNB) and [125I]alpha-bungarotoxin ([1...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Meyer MR,Reddy GR

    更新日期:1985-10-01 00:00:00

  • Irreversible inhibition of calcium uptake in synaptosomes by quinacrine mustard: relationship to labeling sites of quinacrine mustard.

    abstract::The sites of interaction of quinacrine with synaptic membranes were labeled with quinacrine mustard. Quinacrine mustard had an inhibitory effect on depolarization-induced calcium uptake by synaptosomes similar to that of quinacrine. The inhibition of depolarization-induced calcium uptake by quinacrine was reduced by 7...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ohta A,Baba A,Iwata H

    更新日期:1985-08-01 00:00:00

  • Proteasomal inhibition leads to formation of ubiquitin/alpha-synuclein-immunoreactive inclusions in PC12 cells.

    abstract::Proteasomal dysfunction has been recently implicated in the pathogenesis of several neurodegenerative diseases, including Parkinson's disease and diffuse Lewy body disease. We have developed an in vitro model of proteasomal dysfunction by applying pharmacological inhibitors of the proteasome, lactacystin or ZIE[O-tBu]...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Rideout HJ,Larsen KE,Sulzer D,Stefanis L

    更新日期:2001-08-01 00:00:00

  • Astrocyte-neurone communication following oxygen-glucose deprivation.

    abstract::We looked at the possible interactions between astrocytes and neurones during reperfusion using an in vitro model of ischaemia-reperfusion injury, as a controlled environment that lends itself easily to manipulation of the numerous variables involved in such an insult. We constructed a chamber in which O2 can be lower...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Griffin S,Clark JB,Canevari L

    更新日期:2005-11-01 00:00:00

  • Gene-environment interactions in sporadic Parkinson's disease.

    abstract::Much has been learned in recent years about the genetics of familial Parkinson's disease. However, far less is known about those malfunctioning genes which contribute to the emergence and/or progression of the vast majority of cases, the 'sporadic Parkinson's disease', which is the focus of our current review. Drastic...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章,评审


    authors: Benmoyal-Segal L,Soreq H

    更新日期:2006-06-01 00:00:00

  • Activation of glutamate dehydrogenase by leucine and its nonmetabolizable analogue in rat brain synaptosomes.

    abstract::Leucine and beta-(+/-)-2-aminobicyclo[2.2.1]heptane-2-carboxylic acid (BCH) stimulated, in a dose-dependent manner, reductive amination of 2-oxoglutarate in rat brain synaptosomes treated with Triton X-100. The concentration dependence curves were sigmoid, with 10-15-fold stimulations at 15 mM leucine (or BCH); oxidat...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Erecińska M,Nelson D

    更新日期:1990-04-01 00:00:00

  • Intragenic sequences affect the expression of the gene encoding glial fibrillary acidic protein.

    abstract::We show that the expression of the gene encoding glial fibrillary acidic protein (GFAP) gene is affected by at least three cis-acting elements. A positive regulatory element that is located between nucleotides -1,631 and -1,479 can confer cell type-specific expression on a heterologous gene. A second regulatory elemen...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Sarkar S,Cowan NJ

    更新日期:1991-08-01 00:00:00

  • Effect of cocaine on the histaminergic neuron system in the rat brain.

    abstract::To examine the effect of cocaine on the brain histamine neuron system, histamine levels and histamine N-methyltransferase activity in the rat brain were measured after the administration of cocaine. Moreover, we examined the effect of L-histidine on cocaine-induced wheel-running behavior. The administration of cocaine...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ito C,Onodera K,Sakurai E,Sato M,Watanabe T

    更新日期:1997-08-01 00:00:00

  • Protein tyrosine phosphatase σ regulates the synapse number of zebrafish olfactory sensory neurons.

    abstract::The formation and refinement of synaptic connections are key steps of neural development to establish elaborate brain networks. To investigate the functional role of protein tyrosine phosphatase (PTP) σ, we employed an olfactory sensory neuron (OSN)-specific gene manipulation system in combination with in vivo imaging...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Chen X,Yoshida T,Sagara H,Mikami Y,Mishina M

    更新日期:2011-11-01 00:00:00

  • Intracellular pH, lactate, and energy metabolism in neonatal brain during partial ischemia measured in vivo by 31P and 1H nuclear magnetic resonance spectroscopy.

    abstract::Sequential 31P and 1H nuclear magnetic resonance spectra were measured for neonatal piglets (n = 7) to determine the relationship between brain intracellular pH (pHi), lactate, and phosphorylated energy metabolites during partial ischemia. Simultaneous determinations of arterial and cerebral venous blood gases, pH, O2...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Corbett RJ,Laptook AR,Nunnally RL,Hassan A,Jackson J

    更新日期:1988-11-01 00:00:00

  • Specificity of muscarinic acetylcholine receptor regulation by receptor activity.

    abstract::Regulation of muscarinic acetylcholine receptor concentration by receptor activity in neuron-like NG108-15 hybrid cells is a highly specific process. Receptor levels, monitored by binding of [3H]quinuclidinyl benzilate ([3H]QNB), decreased 50--75% following 24-h incubation of cells with muscarinic agonists, but none o...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Siman RG,Klein WL

    更新日期:1981-11-01 00:00:00

  • Influences of dopaminergic lesion on epidermal growth factor-ErbB signals in Parkinson's disease and its model: neurotrophic implication in nigrostriatal neurons.

    abstract::Epidermal growth factor (EGF) is a member of a structurally related family containing heparin-binding EGF-like growth factor (HB-EGF) and transforming growth factor alpha (TGFalpha) that exerts neurotrophic activity on midbrain dopaminergic neurons. To examine neurotrophic abnormality in Parkinson's disease (PD), we m...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Iwakura Y,Piao YS,Mizuno M,Takei N,Kakita A,Takahashi H,Nawa H

    更新日期:2005-05-01 00:00:00

  • Nicotine self-administration differentially modulates glutamate and GABA transmission in hypothalamic paraventricular nucleus to enhance the hypothalamic-pituitary-adrenal response to stress.

    abstract::The mechanisms by which chronic nicotine self-administration augments hypothalamo-pituitary-adrenal (HPA) responses to stress are only partially understood. Nicotine self-administration alters neuropeptide expression in corticotropin-releasing factor (CRF) neurons within paraventricular nucleus (PVN) and increases PVN...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Yu G,Chen H,Wu X,Matta SG,Sharp BM

    更新日期:2010-05-01 00:00:00

  • Specific binding of glycosylated beta-galactosidase to bovine brain synaptic membrane.

    abstract::The binding of a series of glycosylated beta-galactosidases to a fraction rich in synaptic membrane of bovine brain was examined. beta-galactosidase modified with p-aminophenyl beta-D-galactopyranoside (beta-D-Gal beta-gal) was found the most effective in binding to synaptic membrane, followed by that modified with be...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Naoi M,Nagatsu T

    更新日期:1986-02-01 00:00:00

  • Effects of ATP and taurine on calcium uptake by membrane preparations of the rat retina.

    abstract::The effects of ATP and taurine on the kinetics of calcium uptake in rat retinal membrane preparations were determined. ATP increased calcium uptake at low calcium ion concentrations. Addition of ATP plus taurine further increased calcium uptake. Cooperative relationships were observed for calcium uptake in the absence...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Lombardini JB

    更新日期:1983-02-01 00:00:00

  • Ca(2+) -independent vesicular catecholamine release in PC12 cells by nanomolar concentrations of Pb(2+).

    abstract::Effects of Pb(2+) on vesicular catecholamine release in intact and ionomycin-permeabilized PC12 cells were investigated using carbon fibre microelectrode amperometry. Changes in intracellular Pb(2+) and Ca(2+) were measured from indo-1 fluorescence by confocal laser scanning microscopy. Depolarization of intact cells ...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Westerink RH,Vijverberg HP

    更新日期:2002-03-01 00:00:00

  • Reduction of glial glutamate transporters in the parietal cortex and hippocampus of the EL mouse.

    abstract::There is extensive experimental evidence indicating a crucial role for glutamate in epileptogenesis and epileptic activity. The glial glutamate transporters GLT1 and GLAST are proposed to account for the majority of extracellular glutamate re-uptake. In the present study, polyclonal antibodies specific to GLT1 and GLA...

    journal_title:Journal of neurochemistry

    pub_type: 杂志文章


    authors: Ingram EM,Wiseman JW,Tessler S,Emson PC

    更新日期:2001-11-01 00:00:00