Abstract：
:RGS (regulators of G-protein signaling) proteins comprise a large family that modulates heterotrimeric G-protein signaling. This protein family has a common RGS domain and functions as GTPase-activating proteins for the alpha-subunits of heterotrimeric G-proteins located at the plasma membrane. RGS8 was identified as a neuron-specific RGS protein, which belongs to the B/R4 subfamily. We previously showed that RGS8 protein was translocated to the plasma membrane from the nucleus on coexpression of GTPase-deficient Galphao (GalphaoQL). Here, we first examined which subtypes of Galpha can induce the translocation of RGS8. When the Galphai family was expressed, the translocation of RGS8 did occur. To investigate the mechanism of this translocation, we generated a mutant RGS8 with reduced affinity to Galphao and an RGS-insensitive (RGS-i) mutant of GalphaoQL. Co-expression experiments with both mutants revealed that disruption of the Galpha-RGS8 interaction abolished the membrane-translocation of RGS8 despite the apparent membrane localization of RGS-i GalphaoQL. These results demonstrated that RGS8 is recruited to the plasma membrane where G-proteins are activated mainly by direct association with Galpha.
journal_name
J Neurochemjournal_title
Journal of neurochemistryauthors
Masuho I,Itoh M,Itoh H,Saitoh Odoi
10.1046/j.1471-4159.2003.02139.xkeywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
161-8issue
1eissn
0022-3042issn
1471-4159pii
2139journal_volume
88pub_type
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更新日期：1984-12-01 00:00:00
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doi：10.1046/j.1471-4159.1994.63051839.x
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