Endonuclease activity associated with purified PM2 bacteriophages.

abstract：:Cyclotides are plant defense peptides that have been extensively investigated for pharmaceutical and agricultural applications, but key details of their posttranslational biosynthesis have remained elusive. Asparaginyl endopeptidases are crucial in the final stage of the head-to-tail cyclization reaction, but the enzy...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Rehm FBH,Jackson MA,De Geyter E,Yap K,Gilding EK,Durek T,Craik DJ

更新日期：2019-04-16 00:00:00

abstract：:The vertical occipital fasciculus (VOF) is the only major fiber bundle connecting dorsolateral and ventrolateral visual cortex. Only a handful of studies have examined the anatomy of the VOF or its role in cognition in the living human brain. Here, we trace the contentious history of the VOF, beginning with its origin...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yeatman JD,Weiner KS,Pestilli F,Rokem A,Mezer A,Wandell BA

更新日期：2014-12-02 00:00:00

abstract：:The formation of a fracture network is a key process for many geophysical and industrial practices from energy resource recovery to induced seismic management. We focus on the initial stage of a fracture network formation using experiments on the symmetric coalescence of two equal coplanar, fluid-driven, penny-shaped ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Keeffe NJ,Zheng Z,Huppert HE,Linden PF

更新日期：2018-10-09 00:00:00

abstract：:Murine erythroleukemia cells (MELC) are transformed cells that can be induced to differentiate by a variety of agents, such as hexamethylenebisacetamide (HMBA) and dimethyl sulfoxide. Dexamethasone suppresses HMBA-mediated MELC differentiation, but MELC retain a memory for their exposure to HMBA since, on transfer fro...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Murate T,Kaneda T,Rifkind RA,Marks PA

更新日期：1984-06-01 00:00:00

abstract：:The yeast SIN1 protein is a nuclear protein that together with other proteins behaves as a transcriptional repressor of a family of genes. In addition, sin1 mutants are defective in proper mitotic chromosome segregation. In an effort to understand the basis for these phenotypes, we employed the yeast two-hybrid system...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Shpungin S,Liberzon A,Bangio H,Yona E,Katcoff DJ

更新日期：1996-08-06 00:00:00

abstract：:Cosuppression results in the degradation of RNA from host genes and homologous transgenes after transcription in the nucleus. By using grafting experiments, we have shown previously that a systemic signal mediates the propagation of cosuppression of Nia host genes and 35S-Nia2 transgenes from silenced 35S-Nia2 transge...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Palauqui JC,Vaucheret H

更新日期：1998-08-04 00:00:00

abstract：:Cystic fibrosis (CF) is an autosomal recessive disorder, the most common lethal genetic disease in Caucasians. Respiratory disease is the major cause of morbidity and mortality. Indeed, 95% of CF patients die of respiratory failure. Pseudomonas aeruginosa, an opportunistic pathogen, chronically infects the lungs of ov...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li JD,Feng W,Gallup M,Kim JH,Gum J,Kim Y,Basbaum C

更新日期：1998-05-12 00:00:00

abstract：:Myosin II is a molecular motor that converts chemical to mechanical energy and enables muscle operations. After a power stroke, a recovery transition completes the cycle and returns the molecular motor to its prestroke state. Atomically detailed simulations in the framework of the Milestoning theory are used to calcul...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Elber R,West A

更新日期：2010-03-16 00:00:00

abstract：:A DNA fragment homologous to U6 small nuclear RNA was isolated from a human genomic library and sequenced. The immediate 5'-flanking region of the U6 DNA clone had significant homology with a potential mouse U6 gene, including a "TATA box" at a position 26-29 nucleotides upstream from the transcription start site. Alt...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kunkel GR,Maser RL,Calvet JP,Pederson T

更新日期：1986-11-01 00:00:00

abstract：:Peritoneal macrophages were obtained from DBA/2 mice that were untreated or after the injection of bacillus Calmette-Guerin (BCG), thioglycollate broth, proteose-peptone broth, or gamma-irradiated P-815 tumor cells. These macrophages were "activated" to become cytotoxic for a fibroblast cell line (L 929) by the additi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blumenthal EJ,Roberts WK,Vasil A,Talmage DW

更新日期：1983-04-01 00:00:00

abstract：:Ventricular fibrillation is the leading cause of sudden cardiac death. In fibrillation, fragmented electrical waves meander erratically through the heart muscle, creating disordered and ineffective contraction. Theoretical and computer studies, as well as recent experimental evidence, have suggested that fibrillation ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garfinkel A,Kim YH,Voroshilovsky O,Qu Z,Kil JR,Lee MH,Karagueuzian HS,Weiss JN,Chen PS

更新日期：2000-05-23 00:00:00

abstract：:We are designing simple peptide ion channels as model systems for the study of the physical principles controlling conduction through ion-channel proteins. Here we report on an uncharged peptide, Ac-(Leu-Ser-Ser-Leu-Leu-Ser-Leu)3-CONH2, designed to form an aggregate of parallel, amphiphilic, membrane-spanning alpha-he...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kienker PK,DeGrado WF,Lear JD

更新日期：1994-05-24 00:00:00

abstract：:Kinetics of growth and decay of triplet states of molecules at known orientation in a magnetic field have been measured. Amplitudes of the zero field eigenstates in each of the eigenstates at finite field were varied by choice of orientation. No interference between the amplitudes of the zero field states was found in...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Felix CC,Weissman SI

更新日期：1975-11-01 00:00:00

abstract：:The ability to modulate the collective properties of correlated electron systems at their interfaces and surfaces underpins the burgeoning field of "designer" quantum materials. Here, we show how an electronic reconstruction driven by surface polarity mediates a Stoner-like magnetic instability to itinerant ferromagne...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mazzola F,Sunko V,Khim S,Rosner H,Kushwaha P,Clark OJ,Bawden L,Marković I,Kim TK,Hoesch M,Mackenzie AP,King PDC

更新日期：2018-12-18 00:00:00

abstract：:Injection of antisense oligonucleotides was used to investigate the function of c-mos in murine oocytes. Oocytes injected with antisense c-mos oligonucleotides completed the first meiotic division but failed to initiate meiosis II. Instead, loss of c-mos function led to chromosome decondensation, reformation of a nucl...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： O'Keefe SJ,Wolfes H,Kiessling AA,Cooper GM

更新日期：1989-09-01 00:00:00

abstract：:Seed dormancy is a trait of considerable adaptive significance because it maximizes seedling survival by preventing premature germination under unfavorable conditions. Understanding how seeds break dormancy and initiate growth is also of great agricultural and biotechnological interest. Abscisic acid (ABA) plays prima...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lopez-Molina L,Mongrand S,Chua NH

更新日期：2001-04-10 00:00:00

abstract：:The mechanism of contrast enhancement of tumors using magnetic resonance imaging was investigated in MCF7 human breast cancer implanted in nude mice. Dynamic contrast-enhanced images recorded at high spatial resolution were analyzed by an image analysis method based on a physiological model, which included the blood c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Furman-Haran E,Margalit R,Grobgeld D,Degani H

更新日期：1996-06-25 00:00:00

abstract：:The photosynthetic apparatus converts light into chemical energy by a series of reactions that give rise to a coupled flow of electrons and protons that generate reducing power and ATP, respectively. A key intermediate in these reactions is plastoquinone (PQ), the most abundant electron and proton (hydrogen) carrier i...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： McCauley SW,Melis A,Tang GM,Arnon DI

更新日期：1987-12-01 00:00:00

abstract：:Hybrid dysgenesis in Drosophila is a syndrome of gonadal atrophy, sterility, and male recombination, and it occurs in the progeny of crosses between males that harbor certain transposable elements (TEs) and females that lack them. Known examples of hybrid dysgenesis in Drosophila melanogaster result from mobilization ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Blumenstiel JP,Hartl DL

更新日期：2005-11-01 00:00:00

abstract：:The global gene expression profiles for 67 human lung tumors representing 56 patients were examined by using 24,000-element cDNA microarrays. Subdivision of the tumors based on gene expression patterns faithfully recapitulated morphological classification of the tumors into squamous, large cell, small cell, and adenoc...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Garber ME,Troyanskaya OG,Schluens K,Petersen S,Thaesler Z,Pacyna-Gengelbach M,van de Rijn M,Rosen GD,Perou CM,Whyte RI,Altman RB,Brown PO,Botstein D,Petersen I

更新日期：2001-11-20 00:00:00

abstract：:The diversity of the T cell receptor (TCR) repertoire is limited, because of the processes of positive and negative T cell selection. To obtain T cells with specificities beyond the immune system's capacity, we have developed a strategy for retroviral TCR display. In this approach, a library of T cell variants is gene...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kessels HW,van Den Boom MD,Spits H,Hooijberg E,Schumacher TN

更新日期：2000-12-19 00:00:00

abstract：:The glycine receptor (GlyR) is a pentameric ligand-gated ion channel (pLGIC) mediating inhibitory transmission in the nervous system. Its transmembrane domain (TMD) is the target of allosteric modulators such as general anesthetics and ethanol and is a major locus for hyperekplexic congenital mutations altering the al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Moraga-Cid G,Sauguet L,Huon C,Malherbe L,Girard-Blanc C,Petres S,Murail S,Taly A,Baaden M,Delarue M,Corringer PJ

更新日期：2015-03-03 00:00:00

abstract：:K+ channels, which have been linked to regulation of electrogenic solute transport as well as Ca2+ influx, represent a locus in hepatocytes for the concerted actions of hormones that employ Ca2+ and cAMP as intracellular messengers. Despite considerable study, the single-channel basis for synergistic effects of Ca2+ a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lidofsky SD

更新日期：1995-07-18 00:00:00

abstract：:F1-ATPase, the catalytic domain of ATP synthase, synthesizes most of the ATP in living organisms. Running in reverse powered by ATP hydrolysis, this hexameric ring-shaped molecular motor formed by three αβ-dimers creates torque on its central γ-subunit. This reverse operation enables detailed explorations of the mecha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Okazaki K,Hummer G

更新日期：2013-10-08 00:00:00

abstract：:For study of the basis of an X-linked form of gout in man, several clonal lines deficient in hypoxanthine-guanine phosphoribosyltransferase (EC 2.4.2.8) were selected from the human lymphoblast line WI-L2 by spontaneous and mutagen-induced resistance to 10 muM 8-azaguanine. Three groups could be defined: (1) clones wi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lever JE,Nuki G,Seegmiller JE

更新日期：1974-07-01 00:00:00

abstract：:It is unknown whether cross-regulatory interactions between homeotic genes, which have been shown to play an important role in the maintenance of their expression domains during Drosophila development, are also important during mammalian development. We have analyzed here the expression of Hox genes in Hoxa-1 (Hox-1.6...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dollé P,Lufkin T,Krumlauf R,Mark M,Duboule D,Chambon P

更新日期：1993-08-15 00:00:00

abstract：:The fluorescence decay functions of individual, specifically labeled tRNAPhe molecules exhibit nonexponential character as a result of conformational dynamics occurring during the measurement on a single molecule. tRNAPhe conformational states that interchange much more slowly are evidenced by the distribution of life...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jia Y,Sytnik A,Li L,Vladimirov S,Cooperman BS,Hochstrasser RM

更新日期：1997-07-22 00:00:00

abstract：:Refinement of neural circuits in the mammalian cerebral cortex shapes brain function during development and in the adult. However, the signaling mechanisms underlying the synapse-specific shrinkage and loss of spiny synapses when neural circuits are remodeled remain poorly defined. Here, we show that low-frequency glu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Oh WC,Hill TC,Zito K

更新日期：2013-01-22 00:00:00

abstract：:To coordinate movements with events in a dynamic environment the brain has to anticipate when those events occur. A classic example is the estimation of time to contact (TTC), that is, when an object reaches a target. It is thought that TTC is estimated from kinematic variables. For example, a tennis player might use ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chang CJ,Jazayeri M

更新日期：2018-03-20 00:00:00

abstract：:The basic biochemical mechanisms underlying many heritable human polycystic diseases are unknown despite evidence that most cases are caused by mutations in members of several protein families, the most prominent being the polycystin gene family, whose products are found on the primary cilia, or due to mutations in po...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Condac E,Silasi-Mansat R,Kosanke S,Schoeb T,Towner R,Lupu F,Cummings RD,Hinsdale ME

更新日期：2007-05-29 00:00:00