Association of yeast SIN1 with the tetratrico peptide repeats of CDC23.

abstract：:We provide here evidence that supports the occurrence of a biologically dormant form of selectin ligand carbohydrate, the sialyl 6-sulfo Lewis X containing modified sialic acid, in human leukocytes. The modification of sialic acid involves first de-N-acetylation of sialic acid moiety through ubiquitous de-N-acetylatio...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mitsuoka C,Ohmori K,Kimura N,Kanamori A,Komba S,Ishida H,Kiso M,Kannagi R

更新日期：1999-02-16 00:00:00

abstract：:Somatic-cell hybrids have been shown to maintain the correct epigenetic chromatin states to study developmental globin gene expression as well as gene expression on the active and inactive X chromosomes. This suggests the potential use of somatic-cell hybrids containing either a maternal or a paternal human chromosome...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gabriel JM,Higgins MJ,Gebuhr TC,Shows TB,Saitoh S,Nicholls RD

更新日期：1998-12-08 00:00:00

abstract：:Transcription, splicing, and translation are potentially coordinately regulatable in a temporospatial-dependent manner, although supporting experimental evidence for this notion is scarce. Yeast two-hybrid screening of a mammary gland cDNA library with human p21-activated kinase 1 (Pak1) as bait identified polyC-RNA-b...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Meng Q,Rayala SK,Gururaj AE,Talukder AH,O'Malley BW,Kumar R

更新日期：2007-04-03 00:00:00

abstract：:As countries pursue sustainable development across sectors as diverse as health, agriculture, and infrastructure, sectoral policies interact, generating synergies that alter their effectiveness. Identifying those synergies ex ante facilitates the harmonization of policies and provides an important lever to achieve the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pedercini M,Arquitt S,Collste D,Herren H

更新日期：2019-11-12 00:00:00

abstract：:Striking taste disturbances are reported in cancer patients treated with Hedgehog (HH)-pathway inhibitor drugs, including sonidegib (LDE225), which block the HH pathway effector Smoothened (SMO). We tested the potential for molecular, cellular, and functional recovery in mice from the severe disruption of taste-organ ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kumari A,Ermilov AN,Grachtchouk M,Dlugosz AA,Allen BL,Bradley RM,Mistretta CM

更新日期：2017-11-28 00:00:00

abstract：:The virulence of many pathogens depends upon their ability to cope with immune-generated nitric oxide (NO·). In Escherichia coli, the major NO· detoxification systems are Hmp, an NO· dioxygenase (NOD), and NorV, an NO· reductase (NOR). It is well established that Hmp is the dominant system under aerobic conditions, wh...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robinson JL,Brynildsen MP

更新日期：2016-03-22 00:00:00

abstract：:The Cu,Zn superoxide dismutase (SOD) was examined earlier and found to behave in a very unclock-like manner despite (accepted point mutation, or PAM) corrections for multiple replacements per site. Depending upon the time span involved, rates could differ 5-fold. We have sought to determine whether the data might be c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fitch WM,Ayala FJ

更新日期：1994-07-19 00:00:00

abstract：:In this paper I review the ways in which the glassy state is obtained both in nature and in materials science and highlight a "new twist"--the recent recognition of polymorphism within the glassy state. The formation of glass by continuous cooling (viscous slowdown) is then examined, the strong/fragile liquids classif...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Angell CA

更新日期：1995-07-18 00:00:00

abstract：:Nonenzymatic manganese was first shown to provide protection against superoxide toxicity in vivo in 1981, but the chemical mechanism responsible for this protection subsequently became controversial due to conflicting reports concerning the ability of Mn to catalyze superoxide disproportionation in vitro. In a recent ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Barnese K,Gralla EB,Valentine JS,Cabelli DE

更新日期：2012-05-01 00:00:00

abstract：:The high resolution 1H and 31P NMR spectra of the (dG-dC)8 duplex have been recorded in low- and high-salt solutions in order to evaluate the structural aspects of the salt-induced transition of oligo(dG-dC) in solution [Pohl, F. M. & Jovin, T. M. (1972) J. Mol. Biol. 67, 375-396]. The NMR data require that the (dG-d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Patel DJ,Canuel LL,Pohl FM

更新日期：1979-06-01 00:00:00

abstract：:Tikal has long been viewed as one of the leading polities of the ancient Maya realm, yet how the city was able to maintain its substantial population in the midst of a tropical forest environment has been a topic of unresolved debate among researchers for decades. We present ecological, paleoethnobotanical, hydraulic,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lentz DL,Dunning NP,Scarborough VL,Magee KS,Thompson KM,Weaver E,Carr C,Terry RE,Islebe G,Tankersley KB,Grazioso Sierra L,Jones JG,Buttles P,Valdez F,Ramos Hernandez CE

更新日期：2014-12-30 00:00:00

abstract：:To clone the gene for the human Na+/H+ antiporter, we first constructed a stable mouse LTK- cell line (LAP1) lacking Na+/H+ antiport activity. Second, we devised a selective technique based on acid killing that specifically sorts out cells expressing low levels of Na+/H+ antiport activity from a population of antiport...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Franchi A,Perucca-Lostanlen D,Pouyssegur J

更新日期：1986-12-01 00:00:00

abstract：:The ATP-dependent enzyme dethiobiotin synthetase from Escherichia coli catalyses the formation of dethiobiotin from CO2 and 7, 8-diaminopelargonic acid. The reaction is initiated by the formation of a carbamate and proceeds through a phosphorylated intermediate, a mixed carbamic phosphoric anhydride. Here, we report t...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Käck H,Gibson KJ,Lindqvist Y,Schneider G

更新日期：1998-05-12 00:00:00

abstract：:Major histocompatibility complex (MHC) class II molecules displayed clustered patterns at the surfaces of T (HUT-102B2) and B (JY) lymphoma cells characterized by interreceptor distances in the micrometer range as detected by scanning force microscopy of immunogold-labeled antigens. Electron microscopy revealed that a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Jenei A,Varga S,Bene L,Mátyus L,Bodnár A,Bacsó Z,Pieri C,Gáspár R Jr,Farkas T,Damjanovich S

更新日期：1997-07-08 00:00:00

abstract：:Healthy ocean ecosystems are needed to sustain people and livelihoods and to achieve the United Nations Sustainable Development Goals. Using the ocean sustainably requires overcoming many formidable challenges: overfishing, climate change, ocean acidification, and pollution. Despite gloomy forecasts, there is reason f...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lubchenco J,Cerny-Chipman EB,Reimer JN,Levin SA

更新日期：2016-12-20 00:00:00

abstract：:Sympathetic ganglia and some peripheral tissues of adult guinea pig and cat were analyzed by the indirect immunofluorescence technique with antisera to catecholamine-synthesizing enzymes and some peptides. In the guinea pig, noradrenergic neurons could be subdivided into three populations containing respectively (i) s...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lundberg JM,Hökfelt T,Anggård A,Terenius L,Elde R,Markey K,Goldstein M,Kimmel J

更新日期：1982-02-01 00:00:00

abstract：:Bone morphogenetic protein (BMP) signaling plays a major role in dorsoventral patterning in vertebrates and in Drosophila. Remarkably, in Tribolium, a beetle with an ancestral type of insect development, early BMP/dpp exhibits differential expression along the anteroposterior axis. However, the BMP/Dpp inhibitor Sog/c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： van der Zee M,Stockhammer O,von Levetzow C,Nunes da Fonseca R,Roth S

更新日期：2006-10-31 00:00:00

abstract：:Members of the caspase family of cysteine proteases play central roles in coordinating the stereotypical events that occur during apoptosis. Because the major executioner caspases, caspase-3 and caspase-7, exhibit almost indistinguishable activity toward certain synthetic peptide substrates, this has led to the widesp...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Walsh JG,Cullen SP,Sheridan C,Lüthi AU,Gerner C,Martin SJ

更新日期：2008-09-02 00:00:00

abstract：:Apicomplexan parasites replicate within a protective organelle, called the parasitophorous vacuole (PV). The Toxoplasma gondii PV is filled with a network of tubulated membranes, which are thought to facilitate trafficking of effectors and nutrients. Despite being critical to parasite virulence, there is scant mechani...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beraki T,Hu X,Broncel M,Young JC,O'Shaughnessy WJ,Borek D,Treeck M,Reese ML

更新日期：2019-03-26 00:00:00

abstract：:Recent advances indicate that, in various chronic inflammatory disorders, the activation of the immune system is triggered locally rather than in lymphoid organs. In this study, we have evaluated whether the humoral alloimmune response involved in chronic rejection is elicited within the graft. We used the rat aortic ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Thaunat O,Field AC,Dai J,Louedec L,Patey N,Bloch MF,Mandet C,Belair MF,Bruneval P,Meilhac O,Bellon B,Joly E,Michel JB,Nicoletti A

更新日期：2005-10-11 00:00:00

abstract：:On illuminating chloroplasts with "short-wavelength" monochromatic light that supports oxygen evolution, spectral evidence was obtained for a new photoreactive chloroplast component, provisionally designated C550, which shows a reversible decrease of absorbance with a maximum at 550 mmu. The light-induced absorbance c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Knaff DB,Arnon DI

更新日期：1969-07-01 00:00:00

abstract：:We tested the possibility that insulin-like growth factor I (IGF-I) acts as a neuromodulator in the adult cerebellar cortex since previous observations indicated that IGF-I is located in the olivo-cerebellar system encompassing the inferior olive and Purkinje cells. We found that conjoint administration of IGF-I and g...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Castro-Alamancos MA,Torres-Aleman I

更新日期：1993-08-01 00:00:00

abstract：:Climate models show that particles formed by nucleation can affect cloud cover and, therefore, the earth's radiation budget. Measurements worldwide show that nucleation rates in the atmospheric boundary layer are positively correlated with concentrations of sulfuric acid vapor. However, current nucleation theories do ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen M,Titcombe M,Jiang J,Jen C,Kuang C,Fischer ML,Eisele FL,Siepmann JI,Hanson DR,Zhao J,McMurry PH

更新日期：2012-11-13 00:00:00

abstract：:The absence of tools for mapping the forces that drive morphogenetic movements in embryos has impeded our understanding of animal development. Here we describe a unique approach, video force microscopy (VFM), that allows detailed, dynamic force maps to be produced from time-lapse images. The forces at work in an embry...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brodland GW,Conte V,Cranston PG,Veldhuis J,Narasimhan S,Hutson MS,Jacinto A,Ulrich F,Baum B,Miodownik M

更新日期：2010-12-21 00:00:00

abstract：:Accurate calculations of force generated and of ATP flux, in steady isotonic contractions, are made on a simple, two-state muscle model of the sliding filament type. The objective is to illustrate the proper formulation and use of a complete and self-consistent molecular model of muscle. Otherwise, the model is not me...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen YD,Hill TL

更新日期：1974-05-01 00:00:00

abstract：:We propose a model for DNA polymerase fidelity in which free energy differences, delta delta G, between matched and mismatched nucleotides are magnified at the enzyme's active site. Both hydrogen bonding and stacking components of the interaction energy are amplified, with the most profound effect being on the magnitu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Petruska J,Sowers LC,Goodman MF

更新日期：1986-03-01 00:00:00

abstract：:We have studied the effect of the 3' terminal CCA sequence in precursors of tRNAs on catalysis by the RNase P RNA or the holoenzyme from the cyanobacterium Synechocystis sp. PCC 6803 in a completely homologous system. We have found that the absence of the 3' terminal CCA is not detrimental to activity, which is in sha...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Pascual A,Vioque A

更新日期：1999-06-08 00:00:00

abstract：:Chemical labeling of proteins inside of living cells can enable studies of the location, movement, and function of proteins in vivo. Here we demonstrate an approach for chemical labeling of proteins that uses the high-affinity interaction between an FKBP12 mutant (F36V) and a synthetic, engineered ligand (SLF'). A flu...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Marks KM,Braun PD,Nolan GP

更新日期：2004-07-06 00:00:00

abstract：:The unique specificity of monoclonal antibodies (Ab) was combined with the cell-killing function of the cytolytic alternative pathway of complement. The functionally C3b-like glycoprotein of cobra venom was linked to a murine monoclonal Ab directed to a human melanoma-associated antigen by a disulfide bond, by using a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vogel CW,Müller-Eberhard HJ

更新日期：1981-12-01 00:00:00

abstract：:Fluid mixing in active suspensions of microorganisms is important to ecological phenomena and presents a fascinating stochastic process. We investigate the mixing produced by swimming unicellular algal cells (Chlamydomonas) in quasi-two-dimensional liquid films by simultaneously tracking the motion of the cells and th...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kurtuldu H,Guasto JS,Johnson KA,Gollub JP

更新日期：2011-06-28 00:00:00