Fructose 1,6-bisphosphatase: the role of lysosomal enzymes in the modification of catalytic and structural properties.

abstract：:As the Conus shell grows by spiraling of the outer lip around the axis, profound internal shell dissolution thins the walls of the protected penultimate whorl from several millimeters to <50mum. Shell material is added to the inside of the spire and the anterior part of the columella. The resulting shell has a uniform...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kohn AJ,Myers ER,Meenakshi VR

更新日期：1979-07-01 00:00:00

abstract：:Antimicrobial peptides constitute an important component of the mammalian innate immune response. Several types of antimicrobial peptides, including the beta-defensins, are produced at epithelial surfaces in response to infectious threats. Here we show that a class of small molecules, including l-isoleucine and severa...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fehlbaum P,Rao M,Zasloff M,Anderson GM

更新日期：2000-11-07 00:00:00

abstract：:Transcriptional activator proteins in bacteria often operate by interaction with the C-terminal domain of the alpha-subunit of RNA polymerase (RNAP). Here we report the discovery of an "anti-alpha" factor Spx in Bacillus subtilis that blocks transcriptional activation by binding to the alpha-C-terminal domain, thereby...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Nakano S,Nakano MM,Zhang Y,Leelakriangsak M,Zuber P

更新日期：2003-04-01 00:00:00

abstract：:The time-dependent Schrödinger equation is a cornerstone of quantum physics and governs all phenomena of the microscopic world. However, despite its importance, its origin is still not widely appreciated and properly understood. We obtain the Schrödinger equation from a mathematical identity by a slight generalization...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Schleich WP,Greenberger DM,Kobe DH,Scully MO

更新日期：2013-04-02 00:00:00

abstract：:In December of 2015, the international community pledged to limit global warming to below 2 °C above preindustrial (PI) to prevent dangerous climate change. However, to what extent, and for whom, is danger avoided if this ambitious target is realized? We address these questions by scrutinizing heat stress, because the...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Matthews TK,Wilby RL,Murphy C

更新日期：2017-04-11 00:00:00

abstract：:Systemic inflammation occurring around the course of tumor progression and treatment are often correlated with adverse oncological outcomes. As such, it is suspected that neutrophils, the first line of defense against infection, may play important roles in linking inflammation and metastatic seeding. To decipher the d...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Chen MB,Hajal C,Benjamin DC,Yu C,Azizgolshani H,Hynes RO,Kamm RD

更新日期：2018-07-03 00:00:00

abstract：:Cell-based therapies, using multipotent mesenchymal stem cells (MSCs) for organ regeneration, are being pursued for cardiac disease, orthopedic injuries and biomaterial fabrication. The molecular pathways that regulate MSC-mediated regeneration or enhance their therapeutic efficacy are, however, poorly understood. We ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Alfaro MP,Pagni M,Vincent A,Atkinson J,Hill MF,Cates J,Davidson JM,Rottman J,Lee E,Young PP

更新日期：2008-11-25 00:00:00

abstract：:To investigate the role of an anchoring pocket in allele-specific peptide presentation by a major histocompatibility complex class I molecule, we "transplanted" a B pocket from HLA-A*0201 into HLA-B*2705 by site-directed mutagenesis. The resulting protein, designated B27.A2B, binds a different set of endogenous peptid...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Colbert RA,Rowland-Jones SL,McMichael AJ,Frelinger JA

更新日期：1993-07-15 00:00:00

abstract：:As chips further shrink toward smaller scales, fabrication processes based on the self-assembly of individual particles into patterns or structures are often sought. One of the most popular techniques for two-dimensional assembly (self-assembled monolayers) is based on capillary forces acting on particles placed at a ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Aubry N,Singh P,Janjua M,Nudurupati S

更新日期：2008-03-11 00:00:00

abstract：:When an algorithm in dimension one is extended to dimension d, in nearly every case its computational cost is taken to the power d. This fundamental difficulty is the single greatest impediment to solving many important problems and has been dubbed the curse of dimensionality. For numerical analysis in dimension d, we...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Beylkin G,Mohlenkamp MJ

更新日期：2002-08-06 00:00:00

abstract：:Melanomas occur mainly in sunlight-exposed skin. Xeroderma pigmentosum (XP) patients have 1,000-fold higher incidence of melanoma, suggesting that sunlight-induced "bulky" photoproducts are responsible for melanomagenesis. Sunlight induces a high level of reactive oxygen species in melanocytes (MCs); oxidative DNA dam...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Wang HT,Choi B,Tang MS

更新日期：2010-07-06 00:00:00

abstract：:Temporal patterning of biological variables, in the form of oscillations and rhythms on many time scales, is ubiquitous. Altering the temporal pattern of an input variable greatly affects the output of many biological processes. We develop here a conceptual framework for a quantitative understanding of such pattern de...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brezina V,Orekhova IV,Weiss KR

更新日期：1997-09-16 00:00:00

abstract：:We have carried out a study of large malignant skin tumors (squamous cell carcinomas) and other lesions in hairless mice (groups of 38-45) intermittently exposed to ultraviolet light over a period of 15 weeks, beginning when the mice were about 10 weeks old. The several groups were given a standard diet with 0%, 0.3%,...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Dunham WB,Zuckerkandl E,Reynolds R,Willoughby R,Marcuson R,Barth R,Pauling L

更新日期：1982-12-01 00:00:00

abstract：:During differentiation of 3T3-L1 preadipocytes into adipocytes, transcription of the C/EBP alpha (CCA-AT/enhancer binding protein alpha) gene is activated. The promoter of the C/EBP alpha gene contains a bipartite cis element with binding sites for C/EBP alpha undifferentiated protein (CUP) and an Sp1-like GT box bind...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Vasseur-Cognet M,Lane MD

更新日期：1993-08-01 00:00:00

abstract：:The post-translational proteolytic conversion of somatostatin-14 precursors was studied to characterize the enzyme system responsible for the production of the tetradecapeptide either from its 15-kDa precursor protein or from its COOH-terminal fragment, somatostatin-28. A synthetic undecapeptide Pro-Arg-Glu-Arg-Lys-Al...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Gluschankof P,Morel A,Gomez S,Nicolas P,Fahy C,Cohen P

更新日期：1984-11-01 00:00:00

abstract：:The integrity of cell membranes is maintained by a balance between the amount of cholesterol and the amounts of unsaturated and saturated fatty acids in phospholipids. This balance is maintained by membrane-bound transcription factors called sterol regulatory element-binding proteins (SREBPs) that activate genes encod...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Brown MS,Goldstein JL

更新日期：1999-09-28 00:00:00

abstract：:Most current gene transfer methods function satisfactorily in specialized systems involving established cell lines but are often not applicable with nonadherent, primary hematopoietic cells, which are notoriously difficult to transfect. To approach this problem, we have investigated an alternative method of gene trans...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Zenke M,Steinlein P,Wagner E,Cotten M,Beug H,Birnstiel ML

更新日期：1990-05-01 00:00:00

abstract：:Oxygen-derived free radicals have been implicated in the pathogenesis of vasogenic edema and infarction caused by ischemia and reperfusion injury. In earlier studies, exogenously supplied liposome-entrapped CuZn superoxide dismutase (CuZn-SOD) ameliorated ischemic brain edema and infarction in rats following focal cer...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kinouchi H,Epstein CJ,Mizui T,Carlson E,Chen SF,Chan PH

更新日期：1991-12-15 00:00:00

abstract：:Cooperation via production of common goods is found in diverse life forms ranging from viruses to social animals. However, natural selection predicts a "tragedy of the commons": Cheaters, benefiting from without producing costly common goods, are more fit than cooperators and should destroy cooperation. In an attempt ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Waite AJ,Shou W

更新日期：2012-11-20 00:00:00

abstract：:The interleukin 2 (IL-2) receptor (IL-2R) is a multisubunit receptor that includes three major IL-2 binding subunits, the IL-2R alpha, beta, and gamma chains. We have detected and analyzed cooperative interactions between the IL-2R alpha and beta chains (IL-2R alpha and IL-2R beta, respectively) in COS cells transfect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Roessler E,Grant A,Ju G,Tsudo M,Sugamura K,Waldmann TA

更新日期：1994-04-12 00:00:00

abstract：:The reversible binding of the haptens 2,4-dinitrophenyllysine (DNP-lysine) and 2,4,6-trinitrophenyllysine (TNP-lysine) to either the nitrophenyl-binding myeloma protein MOPC-315 or to pooled mouse anti-DNP or anti-TNP antibodies produces large and characteristic extrinsic Cotton effects (in the circular dichroic spect...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Glaser M,Singer SJ

更新日期：1971-10-01 00:00:00

abstract：:The present work shows that arachidonic acid and some other long chain polyunsaturated fatty acids such as docosahexaenoic acid, which is abundant in fish oil, produce a direct open channel block of the major voltage-dependent K+ channel (Kv1.5) cloned in cardiac cells. The inhibitory action of these selected fatty ac...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Honoré E,Barhanin J,Attali B,Lesage F,Lazdunski M

更新日期：1994-03-01 00:00:00

abstract：:Our long-term goal is the in vivo expression of intrinsically colored proteins without the need for further posttranslational modification or chemical functionalization by externally added reagents. Biocompatible (Aza)Indoles (Inds)/(Aza)Tryptophans (Trp) as optical probes represent almost ideal isosteric substitutes ...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Lepthien S,Hoesl MG,Merkel L,Budisa N

更新日期：2008-10-21 00:00:00

abstract：:We report here the crystal structure of the RuvB motor protein from Thermus thermophilus HB8, which drives branch migration of the Holliday junction during homologous recombination. RuvB has a crescent-like architecture consisting of three consecutive domains, the first two of which are involved in ATP binding and hyd...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Yamada K,Kunishima N,Mayanagi K,Ohnishi T,Nishino T,Iwasaki H,Shinagawa H,Morikawa K

更新日期：2001-02-13 00:00:00

abstract：:Rheumatoid synovial cell monolayers, with [1-14C]arachidonic acid ([1-14C]AA) incorporated into cell lipids, are stimulated by a factor (RSF) produced by explant cultures of rheumatoid synovial tissue to produce up to 50-fold increases in [1-14C]prostaglandin E2 and [1-14C]prostaglandin I2. In contrast, levels of free...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Robinson DR,Bastian D,Hamer PJ,Pichiarallo DM,Stephenson ML

更新日期：1981-08-01 00:00:00

abstract：:Viperin is an IFN-inducible radical S-adenosylmethionine (SAM) enzyme that inhibits viral replication. We determined crystal structures of an anaerobically prepared fragment of mouse viperin (residues 45-362) complexed with S-adenosylhomocysteine (SAH) or 5'-deoxyadenosine (5'-dAdo) and l-methionine (l-Met). Viperin c...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Fenwick MK,Li Y,Cresswell P,Modis Y,Ealick SE

更新日期：2017-06-27 00:00:00

abstract：:Growth inhibition assays indicated that the IC50 values for methotrexate (MTX) and 5-fluorodeoxyuridine (FdUrd) in HS-18, a liposarcoma cell line lacking retinoblastoma protein (pRB), and SaOS-2, an osteosarcoma cell line with a truncated and nonfunctional pRB, were 10- to 12-fold and 4- to 11-fold higher, respectivel...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Li W,Fan J,Hochhauser D,Banerjee D,Zielinski Z,Almasan A,Yin Y,Kelly R,Wahl GM,Bertino JR

更新日期：1995-10-24 00:00:00

abstract：:Unconventional myosin 15 is a molecular motor expressed in inner ear hair cells that transports protein cargos within developing mechanosensory stereocilia. Mutations of myosin 15 cause profound hearing loss in humans and mice; however, the properties of this motor and its regulation within the stereocilia organelle a...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Bird JE,Takagi Y,Billington N,Strub MP,Sellers JR,Friedman TB

更新日期：2014-08-26 00:00:00

abstract：:Linkage disequilibrium between polymorphisms in a natural population may result from various evolutionary forces, including random genetic drift due to sampling of gametes during reproduction, restricted migration between subpopulations in a subdivided population, or epistatic selection. In this report, we present evi...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Kirby DA,Muse SV,Stephan W

更新日期：1995-09-26 00:00:00

abstract：:Metabolic, infectious, and tumor cell-intrinsic noxae can all evoke the endoplasmic reticulum (ER) stress response in tumor cells, which is critical for tumor cell growth and cancer progression. Evidence exists that the ER stress response can drive a proinflammatory program in tumor cells and macrophages but, to our k...

journal_title：Proceedings of the National Academy of Sciences of the United States of America

authors： Mahadevan NR,Rodvold J,Sepulveda H,Rossi S,Drew AF,Zanetti M

更新日期：2011-04-19 00:00:00