Abstract:
:We designed experiments to assess the effect of chlorine injury on the virulence of waterborne enteropathogens. Higher chlorine doses (0.9 to 1.5 mg/liter) were necessary to produce injured Yersinia enterocolitica, Salmonella typhimurium, and Shigella spp. than to produce injured enterotoxigenic Escherichia coli or coliform bacteria (0.25 to 0.5 mg/liter) in the test system used; 50% lethal dose experiments in which mice were used showed that injured Y. enterocolitica cells were 20 times less virulent than uninjured control cells (3,300 and 160 CFU, respectively). This decrease in virulence was not related to reduced attachment to Henle 407 intestinal epithelial cells, but could be related to a loss of HeLa cell invasiveness. In contrast, injured S. typhimurium and enterotoxigenic E. coli cells lost their ability to attach to Henle cells. These data show that some enteropathogens and coliform bacteria differ in their sensitivities to chlorine injury and that the virulence determinants affected by chlorine may vary from one pathogen to another.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
LeChevallier MW,Singh A,Schiemann DA,McFeters GAdoi
10.1128/AEM.50.2.412-419.1985subject
Has Abstractpub_date
1985-08-01 00:00:00pages
412-9issue
2eissn
0099-2240issn
1098-5336journal_volume
50pub_type
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更新日期:1992-03-01 00:00:00