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Urotensin II lowers cytoplasmic free calcium concentration in goby enterocytes: measurements using quin2.

Abstract：

:A technique is described for the measurement of cytoplasmic free calcium concentration ([Ca2+]c) in a suspension of goby intestinal epithelial cells using the fluorescent probe quin2. For 18 cell suspensions, [Ca2+]c was determined to be 142 +/- 14 nM. The caudal neurosecretory peptide urotensin II at 10(-7) to 10(-6) M concentration reduced [Ca2+]c by 20-50%. Together with previous findings linking the calcium-calmodulin system with the stimulation of Na+ and Cl- absorption in this tissue, it appears that alterations in cytoplasmic Ca2+ may mediate urotensin II-induced stimulation of ion transport.

journal_name

Gen Comp Endocrinol

journal_title

General and comparative endocrinology

authors

Loretz CA,Assad JA

doi

10.1016/0016-6480(86)90068-7

subject

Has Abstract

pub_date

1986-12-01 00:00:00

pages

355-61

issue

3

eissn

0016-6480

issn

1095-6840

journal_volume

64

pub_type

杂志文章

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